Fungal endophytes colonize an important niche within the plants through secretion of secondary metabolites. The metabolites and extracellular enzymes produced by endophytic fungi regulate the growth of the host plant and contribute in defence mechanisms.The medicinal plants Caralluma acutangula and Boswellia sacra were used for the isolation of endophytic fungi. The endophytic fungi were identified as Penicillium citrinum, Paecilomyces variotii, Aspergillus nidulans, Fusarium oxysporum, Epicucum nigram, Penicillium purpurogenum, Penicillium spinulosum, Aspergillus caespitosus, Phoma and Alternaria sp. and were assessed for their potential to produce anti-cancerous metabolites by performing MTT assay and extracellular enzymes such as cellulases, phosphatases and glucosidases in growth media. P. variotii, P. citrinum and F. oxysporum showed significantly higher amount of phosphatases and glucosidases as compared to other strains. Additionally, P. variotii and F. oxysporum showed significantly higher potential of indole acetic acid production (tryptophan-dependent and independent pathways). ACC (1-Aminocyclopropane-1-carboxylate) deaminase results showed that P.citrinum, P. purpurogenum and P. Variotii had shown prominent ACC deaminase activity (300 nmol α- ketobutyrate mg-1h-1). Fluorescence-based MUB (4-methyl umbelliferone) standards were used to analyze the presence of extracellular enzymes glucosidase, phosphatase and cellulase. The bioactive secondary metabolites from endophytic P. citrinum also revealed some prominent results by performing MTT assay on breast cancer cell line (MCF-7). The current study concludes that these fungi are producing bioactive constituents that could provide unique niche of ecological adaptation by symbiosis and greatly contributing to the healthy life of their host plant. However, some of the endophytic fungi offer a great potential to produce anti-cancerous metabolites and extracellular enzymes.
شکرانہ ایس کتاب دی ترتیب تے تیاری وچ میرے نال جیہڑے جیہڑے مہرباناں نیں تعاون کیتا اے اوہناں وچ ممتاز قانون دان میاں سعید احمد ایڈووکیٹ ضلع کچہری اوکاڑہ، میاں وحیدالدین عرضی نویس ضلع کچہری اوکاڑہ، میاں مسعود الحسن گنج قادری ، حاجی منیر احمد الحمد آئل ملز آف قبولہ شریف ، پروفیسر محمد حسین لنگاہ ڈگری کالج بہاول نگر،سید انیس الرحمن گیلانی تے سب توں ودھ کے اعجاز احمد کمپیوٹر والیاں دا وی بے حد تعاون شامل حال رہیا تے بہاول نگر دی معروف شخصیت میاں علی احمد سنگلہ صاحب جنہاں دا رقم نال مالی تعاون مثالی رہیا۔ میں اوہناں بھراواں دا بڑا شکر گزار ہاں پئی انہاں دی مدد تے معاونت دے نال اے کتاب عملی طور تے چھپ کے ساڈیاں ہتھاں وچ موجود اے۔ اللہ پاک اوہناں دوستاں دے علم ، عمل تے عمر وچ خیرو برکت عطا فرماوے۔(آمین) اقبال قادری
Class Action Research (CAR) was conducted to analyze the increase of interest, creativity and interest, creativity, and learning outcomes of students in the Mattayeom 4 class Phatanakansuksa Foundation School Thailand by implementing the Scientific approach method. During the observation and review with the English teacher Mattayeom 4, the researcher was able to describe the profile of student learning outcomes in grade 1 as a class with great potential but not well-honed. The low interest of students in this learning process can result in a learning process that is not optimal so that the results obtained are not optimal. The results of the implementation of classroom action research in the Mattayeom 4/2 Phatanakansuksa Foundation School's Real Work Lecture program which shows an increase in learning outcomes through a direct learning process applied by teachers/researchers.
Taxonomy is an effective means of organizing, managing and accessing large amounts of data. Data these days is however, changing at a rapid pace. Taxonomy represents theme inherent in data. Taxonomy needs to be evolved to reflect changes occurring in data, otherwise, it maynot represent the theme of the underlying data accurately. Existing taxonomy generation techniques pay less attention to the changing nature of data. Evolution of taxonomy for changing data can be handled either non-incrementally or incrementally. Non-incremental taxonomy evolution process reruns the whole taxonomy generation process from scratch and replaces the existing taxonomy with a new one. Majority of the existing taxonomy generation techniques are handling the evolution of taxonomy non-incrementally. Incremental taxonomy evolution, on the other hand, tries to accommodate changes occurring in data on the existing taxonomy without rerunning the whole taxonomy generation process from scratch. The generation from scratch can make the nonincremental taxonomy evolution a time inefficient and computationally expensive choice as compared to incremental evolution. However, a limited number of existing techniques have focused on the incremental evolution of taxonomy.This work proposes a novel Taxonomy Incremental Evolution (TIE) technique that can evolve an existing taxonomy by incrementally updating it whenever new documents are added in data. The TIE technique relies on a clustering-based taxonomy generation technique for the generation of initial taxonomy and then evolves the existing taxonomy afterward whenever changes in underlying data occur. However, it does not depend on any specific clustering technique.When new documents arrive, the TIE technique first identifies the closest cluster for each of the new documents to get adjusted in. It then checks the impact on cluster quality for the possible adjustment of new documents. In case the cluster quality does not deteriorate, new documents get simply merged in the cluster. However, in the case of quality deterioration, the impact of new documents on the cluster quality is identified by manipulating range of closeness of documents with the cluster. Based on the range of closeness of new documents, restructuring of the existing clusters is performed to adjust new documents, ultimately resulting in an evolved taxonomy. The TIE technique was compared with different non-incremental and incremental taxonomy evolution techniques based on time and quality parameters. Since the focus of this work is on unstructured textual data, so a text dataset of scholarly articles from the computing domain was selected for evaluation. The time-based evaluation clearly shows that the TIE technique takes comparatively less time to achieve evolution of taxonomy. The quality-based evaluation compares the lexical and hierarchical quality of the evolved taxonomy with the reference taxonomy. It was found that the lexical quality of TIE is overall better in comparison to both the non-incremental and incremental counterparts. However, hierarchical quality of the taxonomy evolved using TIE is lower especially in comparison to non-incremental taxonomy evolution techniques. The significance of the obtained results was also analyzed statistically using the t-test. The outcome of the t-test also supports the observations related to time and quality-based evaluation of TIE. Moreover, time and quality metrics were combined in a single metric of quality-time ratio to get an overall idea of the performance. It was found that the rate of improvement in taxonomy quality per unit time is the most in case of TIE as compared to other techniques. However, the qualitytime ratio also shows performance deterioration of TIE with the increasing size of the dataset. This aspect was then further investigated through sensitivity analysis. The result of the sensitivity analysis shows that the TIE technique is performing better when the arrival of new data is in small chunk. Thus, the scalability aspect of the TIE technique can be improved in future.