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Anti-Cancerous Metabolites and Extracellular Enzyme Production by Endophytic Penicillium and Paecilomyces Strains

Thesis Info

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Author

Sajid Ali

Program

PhD

Institute

University of Peshawar

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9770/1/Final%20corrected%20Thesis%2024517.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725506371

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Fungal endophytes colonize an important niche within the plants through secretion of secondary metabolites. The metabolites and extracellular enzymes produced by endophytic fungi regulate the growth of the host plant and contribute in defence mechanisms.The medicinal plants Caralluma acutangula and Boswellia sacra were used for the isolation of endophytic fungi. The endophytic fungi were identified as Penicillium citrinum, Paecilomyces variotii, Aspergillus nidulans, Fusarium oxysporum, Epicucum nigram, Penicillium purpurogenum, Penicillium spinulosum, Aspergillus caespitosus, Phoma and Alternaria sp. and were assessed for their potential to produce anti-cancerous metabolites by performing MTT assay and extracellular enzymes such as cellulases, phosphatases and glucosidases in growth media. P. variotii, P. citrinum and F. oxysporum showed significantly higher amount of phosphatases and glucosidases as compared to other strains. Additionally, P. variotii and F. oxysporum showed significantly higher potential of indole acetic acid production (tryptophan-dependent and independent pathways). ACC (1-Aminocyclopropane-1-carboxylate) deaminase results showed that P.citrinum, P. purpurogenum and P. Variotii had shown prominent ACC deaminase activity (300 nmol α- ketobutyrate mg-1h-1). Fluorescence-based MUB (4-methyl umbelliferone) standards were used to analyze the presence of extracellular enzymes glucosidase, phosphatase and cellulase. The bioactive secondary metabolites from endophytic P. citrinum also revealed some prominent results by performing MTT assay on breast cancer cell line (MCF-7). The current study concludes that these fungi are producing bioactive constituents that could provide unique niche of ecological adaptation by symbiosis and greatly contributing to the healthy life of their host plant. However, some of the endophytic fungi offer a great potential to produce anti-cancerous metabolites and extracellular enzymes.
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ایٹمی پروگرام کا خالق

ایٹمی پروگرام کا اصل خالق کون

چئیر مین بھٹو شہید کی پھانسی کی اصل وجہ کیا تھی اور ضیاء الحق کو ایٹمی پروگرام کا کریڈٹ ؟

 

کبھی یہ سوچنا تم نے کیا کیا لوگو

یہ تم نے کس کو سرِ دار کھو دیا لوگو

یہ نظم اٹک سے تعلق رکھنے والے سابق سینیٹر شاعر دانشور احمد وحید اختر مرحوم کی ہے ۔

تو ہراساں نہ ہوا ظلم کے ایوانوں سے

تو نے جنگ لڑی وقت کے فرعونوں سے

سے قائد عوام اے قائدِ عوام

 

فلسفہ احکام میراث

This research article explores the rationale behind Islamic injunctions regarding inheritance. Unlike other Islamic injunctions, which are briefly enunciated in the Quran but elaborated in Sunnah, inheritance has been detailed in considerable length in the Quranic text itself. This coupled with numerous Prophetic traditions underpins the unique importance Islam accords to the question of inheritance. However, despite its exceptional importance, the subject of Islamic law of inheritance remains mostly a neglected one, even among the students of Islamic seminaries and Ulema. Resultantly, Islam’s brilliant system of inheritance is often not implemented by the adherents of Islam, much to the miseries and hardships of the legal heirs, especially the children and women. Thus these marginalized segments of society are deprived of their rights today just as they were treated before the advent of Islam. This research brings home the fact that the divinely ordained Islamic injunctions of inheritance are based on sound rationale and justification in the best interest of humanity, and that the believers must adhere to these injunctions that are based on three key principles: proximity in relationship, need, and distribution of wealth. The paper explains in great length the types of relatives and legal heirs, the principles of distribution among them, the justification for such shares, and the limits imposed by Quran and Sunnah with regard to the right of the deceased, the heirs, relatives and the state. It also discusses some of the contentious issues in contemporary debate on Islam: an orphan grandson’s title to inheritance, and the philosophy behind 2: 1 inheritance distribution formula between son and daughter. In doing so, the author has not only relied on the main sources of Islamic jurisprudence viz. Quran and Sunnah, in addition to classical and modern Islamic scholarship but also sound argumentation and logical exposition.

Dynamics of Low-Frequency Electromagnetic Waves and Nonlinear Solitary Structures in the Earth’S Ionosphere

The thesis addresses the dynamics of low-frequency planetary electromagnetic (EM) waves and nonlinear solitary structures (vortices and zonal flows) in the Earth’s weakly ionized ionosphere of the E- and F-layers. The propagation of EM inertio-gravity waves in the partially ionized ionospheric E- and F-layers is considered in the shallow water approximation. Accounting of the field-aligned current is the main novelty of the investigation. Existence of two new eigen-frequencies for fast and slow EM waves is revealed in the ionospheric E-layer. It is shown that in F-layer slowly damping new type of inertial-fast magnetosonic waves can propagate. Slowly damping low-frequency oscillations connected with the field-aligned conductivity are found. Broad spectrum of oscillations is investigated. Existence of the new type of coupled EM ULF waves in the Earth’s ionospheric E-layer is revealed. It is shown that in the weakly ionized E-layer along with the prevalent effect of Hall conductivity the latitudinal inhomogeneity of both the Coriolis parameter and the geomagnetic field becomes essential for the ULF waves under the consideration. Action of these effects leads to the coupled propagation of EM Rossby and Khantadze modes on the one hand and coupled propagation of Rossby, Alfvén and Khantadze modes on the other hand. As a result the new type of dispersive Alfvén waves is revealed. Linear propagation properties of such coupled waves are given in detail. Possibility of existence of the other new coupled Internal-gravity and Alfvén EM planetary waves in the weakly ionized ionospheric E-layer is shown. Under such coupling new type of Alfvén waves is revealed. Simplified set of nonlinear equations describing the dynamics of mentioned coupled EM modes in the conductive weakly ionized E-layer is obtained: 1) spatially 2D equations in case of coupled Rossby-Khantadze and coupled Internal-gravity-Alfvén, and 2) spatially 3D equations in case of coupled Rossby-Alfvén-Khantadze modes, respectively. The possibility of self-organization of Rossby-Khantadze modes turbulent state into the nonlinear solitary vortical structures of dipole type in the non-dissipative ionosphere is shown analytically. Conditions for such self-organization are given. It is revealed that nonlinear large-scale vortices may generate the stronger pulses of the geomagnetic field (of the order of (10 4 - 10 5 nT ) ) than the corresponding linear waves. Nonlinear instability of short wavelength turbulence of mentioned coupled Rossby- Khantadze, Internal-Gravity-Alfvén, and Rossby-Alfvén-Khantadze EM planetary modes with respect to the excitation of low-frequency and large-scale perturbations of sheared zonal flow and magnetic field is revealed. The nonlinear mechanism of the instability is driven by the advection of vorticity and is based on the parametric excitation of zonal flow by four finite-amplitude coupled modes leading to the inverse energy cascade toward the longer wavelength. The corresponding driving forces along with the Reynolds stresses are stipulated by Maxwell’s stresses also. The growth rates of the corresponding instability and the conditions for driving them are determined. The possibility of generation of the intense mean magnetic field (of the order of (10 2 - 10 3 ) nT ) is shown. Obtained results undoubtedly will be useful for the interpretation of different observations on vortical motions, sheared zonal flows and magnetic field disturbances in laboratory and astrophysical plasmas.