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Home > Anticarcinogenic Activity of Terpeniod Containing Plants Against Induced Hepatocellular Carcinoma Hcc

Anticarcinogenic Activity of Terpeniod Containing Plants Against Induced Hepatocellular Carcinoma Hcc

Thesis Info

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Author

Malik, Shazia Kanwal

Program

PhD

Institute

Lahore College for Women University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10294/1/Shazia%20Kanwal%20Malik_Botany_2018_LCWU_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725506465

Similar


Hepatocellular Carcinoma is 6th most predominantly existing cancer and second largest contributor to human mortality all over the world. This study is focused on identification of ―Potent Anticancer terpenes‖ from selected experimental plants that may be successfully practicized in cancer chemotherapeutic mainstream. Twenty five plants were taken under consideration for present piece of work. Ethanolic extracts of different parts of these plants were prepared. Standard analytical methods including UV-VIS Spectroscopy, FTIR and GCMS effectively confirmed presence of a large number of secondary metabolites i.e. Flavonoids, Phenols, Terpenoids, Quinine, Saponins and Alkaloids in these extracts. Quenching potential of ethanolic extracts against DPPH free radical fabricated possible antioxidant dynamics of selected experimental plants. During present work we focused on the role of identified plant ―Terpenoids‖ involved in Hepatocurative as well as Hepatoprotective activities. The ethanolic extracts and these identified effective anticancer ―terpenes‖ were used for further experimentation on Hepatocellular Carcinoma in O. cuniculus. In vitro cytotoxic effects of ethanolic extracts of experimental plants and identified ―terpenes‖ (Hexadecanoic acid, methyl ester, Methyl decanoate, n- Hexadecanoic acid ) were assessed by MTT (3-[4, 5-dimethylthiazole-2-yl]-2, 5-diphenyl-tetrazolium bromide) assay against Mouse Fibroblasts (3T3) cell lines along with their IC50 calculation. Hepatocellular Carcinoma was induced by CCl4 administration in O. cuniculus. The safe doses of CCl4, ethanolic extracts and identified ―terpenes‖ were also optimized during this piece of work. Hepatocurative and Hepatoprotective potentials of plants extracts and their identified ―terpenes‖ along with standard drugs (in medical use for treatment of Hepatocellular Carcinoma) were effectively analysed by monitoring physiological changes (pH of saliva, temperature, blood glucose, body weight) in induced Hepatic Carcinomic O. cuniculus. Furthermore, Hematological investigations (Hb, RBC, TLC, MCV, MCH, MCHC and Platelets), changes in Liver Biochemical Enzymes (Alkaline phosphatase, Aspartate amino Transferase and Alanine aminotransferase) and Hepatic Carcinoma Marker (Alpha Fetoprotein) efficiently marked Therapeutic as well as Prophylactic outcomes followed by Histo-Pathological studies of Carcinomic and healthy livers following Hematoxylin and Eosin Staining. xxiii The research work was aimed to critically examine potential role of naturally occurring ―Terpenoids‖ from diverse botanical origins for chemoprevention as well as chemotherapy of liver tumors. Both in vitro and in vivo effects were noted and recorded. We successfully identified the anticancer ―terpenes‖ giving better and non-toxic results against Hepatocellular Carcinoma in comparison to synthetic anti-cancerous drugs in market. In addition to this, we also recorded similar anticancerous response of ethanolic extracts of selected experimental plants against induced Hepatocellular Carcinoma in O. cuniculus. The results of the present piece of work are promising and may be very helpful for finding green, cost effective and eco-friendly chemopreventive and chemotherapeutic measures to deal successfully with liver carcinomas.
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سر ولیم کروکس

سر ولیم کروکس

            ماہ گذشتہ میں علمی دنیا کے لیے سب سے اہم حادثہ یہ ہوا کہ کیمسٹری کے استاد اعظم سر ولیم کروکس نے وفات پائی، موصوف کا شمار اس وقت دنیا کے ممتاز ترین علمائے سائنس میں تھا، اور ممالک برطانیہ میں تو یقینا ان سے بڑے درجہ کا کوئی شخص اس وقت نہ تھا، کیمسٹری میں ہیلیم کا عنصر انہیں نے دریافت کیا اس کے علاوہ ان کے متعدد اکتشافات تھے، جدید اہل سائنس کے گروہ میں شاید وہ پہلے شخص تھے جو عالم ’’روحانیات‘‘ کے وجود کے قائل ہوئے۔ (’’مولوی عبدالماجد‘‘،جون ۱۹۱۹ء)

مکالمہ بین المذاہب شرائط، مقاصد جديد

Now the whole world is the global village. The science has brought revolution in every field of life. Man has made a lot of things for his comforts, discomforts, reliefs and destruction. Now the time has come for us to take evolutionary steps to save us from giant’s destruction, terrorism, extremism, intolerance and unrest which are the machinations of complete annihilation over the earth, which needs cosmopolitanism on our part For this purpose, dialogue can play a vital role. People belongs to different civilizations, cultures, societies, nations and religions can sit around the table beings humans and can solve religious, political, geo political and economical problems by enhancing mutuality and common understanding. Dialogical aim should be to avoid clash of civilizations and peace for everyone. Terrorism and extremism should be stopped and we should promulgate coexistence and peace for whole human community. Purpose should be to serve the humanity. We should promote love, peace, respect for the other religions; serve humanity create equality and equity among human beings. This should be safety of present and coming generation. We should pay our role as super creature and thinking creature; super. The barriers which come across while promulgating cosmopolitanism and expatriation is seemed to be is the double standard of the west, that promotes colonialism, terrorism, extremism and economical support of missionaries and these things are the real problems for those human beings who take serious trenchant and serious efforts to achieve dialogical goals cannot achieve it. The role western media plays; is not supportive to this nation. Western world especially American should use her hegemony to make world worth living and worth seeing to the coming generation.

Development of Relationships Among Vehicular and Driver’S Characteristics With Traffic Accidents

The city of Lahore is the provincial headquarter of the Punjab province. Lahore has different types of traffic as compared to other cities of Pakistan. The old areas have mixed traffic and the outskirt areas have fast moving traffic. The main factors for traffic accidents are roads, vehicles, drivers, external factors and enforcement by traffic police. The riding quality of roads of Lahore is normal. The external factors such as weather condition in Lahore are not so severe as to cause accidents. The condition of vehicles especially public transport fleet, plying on the roads of Lahore, is alarming as periodic maintenance of vehicles is not frequently carried out. The literacy rate of the country is also an issue. Most of the drivers, especially drivers of public transport vehicles, are not educated. They have problems in understanding the traffic rules, signs and signals. In such a situation their behaviour while driving is unsafe and they come across traffic accidents associated with human losses, human sufferings and economic losses. Considering these facts into account it was planned to study the drivers and vehicles characteristics with special reference to their role towards traffic accidents. The basic objective of this research was to develop Accident Prediction Models (APMs) relating number of accidents occurred per year and to know the causes of accidents of different vehicle types based on the vehicular and driver’s characteristics. By knowing the causes of accidents, another purpose was to suggest some measures to lessen the number of accidents. In this regard, questionnaire/survey forms comprising about 107 vehicular and driver’s characteristics were prepared after going through the world wide relevant literature. Seven types of vehicles were selected for the study and for each vehicle type survey was conducted on different locations of Lahore to collect representative information regarding vehicular and driver’s characteristics including number of traffic accidents experienced. The survey locations were chosen on the basis of most probable presence of different vehicle types within Lahore. The types of vehicles considered for this research study include Motorcycle Rickshaw (Qingqi), Motorcycle, Passenger Car, Wagon, Bus, Auto Rickshaw and Pickup. Representative and justified sample sizes were selected using stratified sampling technique on the basis of number of registered vehicles for each type. Statistical package for social sciences (SPSS) was used for analysis of the characteristics. Frequency, correlation and cross-tabulation analyses were conducted for each vehicle. On the basis of these analyses, the most significant driver’s and vehicular characteristics contributing traffic accidents were identified for each vehicle type. With these most significant characteristics, APMs using the multiple linear regression analysis relating the number of accidents per year were developed for all seven types of vehicles. The APMs developed can be used to assess the number of accidents per year for seven types of vehicles on the basis of their vehicular and driver’s characteristics for each vehicle type. On the basis of developed APMs, it is concluded that the main contributing factors towards accidents are vehicular and driver’s characteristics. So it is recommended that by properly addressing the vehicular and driver’s characteristics, incidents of traffic accidents can be reduced.