رئیس احمد جعفری
پچھلے دنوں پاکستان میں ہمارے ایک اورفاضل اورعزیز دوست رئیس احمد جعفری کابھی انتقال ہوگیا۔مرحوم نے تعلیم ندوہ اورجامعہ دونوں میں پائی تھی لیکن تکمیل کسی ایک جگہ بھی نہیں کی۔تحریر کاشوق اور سلیقہ شروع سے تھا اورآدمی تھے بڑے ذہین اور طباع! اس لیے اپنے مطالعہ اورمحنت کے بل بوتہ پر اردوزبان کے نامور ادیب اورمصنف بن گئے۔ ان کاقلم ہی ان کے لیے ذریعہ معاش تھا، اس لیے اگرچہ کوئی ڈھنگ کاعلمی اورتحقیقی کام نہیں کرسکے لیکن افسانہ وناول سے لے کرمذہب اورتاریخ وفلسفہ تک ہر موضوع پر چھوٹی بڑی کتابیں اس کثرت سے لکھی ہیں کہ اس وصف خاص میں ان کاکوئی معاصر ہمسری کادعویٰ نہیں کرسکتا۔ان کاقلم بڑاشگفتہ اوررواں دواں تھا ان کے ساتھ میراذاتی تعلق یہ تھا کہ مرحوم کے نانا سید امتیاز احمد صاحب (ریاض خیرآبادی مرحوم کے حقیقی بھائی) آگرہ میں کوتوال شہرتھے۔ان میں اور میرے والدصاحب قبلہ میں نہایت گہرے برادرانہ تعلقات تھے ۔کوتوال صاحب کے متعلقین ہمارے ہاں آتے اور ہفتوں قیام کرتے تھے ۔ اسی طرح ہم لوگ ان کے ہاں جاتے اور ہفتہ دو ہفتہ ٹھہرتے تھے۔رئیس احمد جعفری کوتوال صاحب (جن کومیں خالو کہتا تھا)کی پہلی بیوی کی لڑکی کے بطن سے تھے۔ان تعلقات کے باعث مرحوم جب تک دہلی میں رہے برابر آتے جاتے رہے ۔جب کبھی ملتے تھے بڑی محبت سے پیش آتے تھے۔ آخری مرتبہ دہلی آئے اور میں ان دنوں ہندوستان سے باہر تھاتولاہور جاتے ہوئے دفتر برہان میں میرے نام ایک خط چھوڑ گئے جس میں لکھا تھا بڑی حسرتوں اور تمناؤں کے ساتھ آیاتھا کہ تم سے ملاقات ہوگی مگر مایوس جارہاہوں۔اب میں بیمار رہنے لگاہوں ، نہ جانے کتنی اور باقی ہے۔البتہ یہ تمنا ضرور ہے کہ مرنے سے پہلے ایک مرتبہ تمہیں اور دیکھ لوں۔‘‘ صدحیف تمنا پوری نہیں ہوئی،اور وہ خدا کو پیارے...
Research on gender roles in relation to government bureaucratic structures is still minimal. This study aims to find answers to the main problem, namely how the role of women in the local government bureaucracy seen from human resource development aspect in Central Sulawesi Province. This research is a qualitative descriptive study which aims to create a picture of the gender presentation in the bureaucratic staffing and the role of women. The subjects of this study were women who hold echelons. The data collection technique used in this study was literature review. For the validity of the data, the cross check technique was used. The data analysis technique used is the inductive analysis technique. The results showed that the role of women in the local government bureaucracy in Central Sulawesi was still not fully implemented. The echelon holder ratio is still dominated by men. However, it is possible that this ratio will change over time.
Xylanases degrade the hemicellulosic component of plant biomass and find potential applications in poultry, paper, textile and biofuel industries. In this study, a novel, family GH10 enzyme, Xyn10B.CB3B2 from Acidothermus cellulolyticus 11B was characterized. This enzyme was found to be a trifunctional enzyme having endo xylanase, arabinofuranosidase and acetyl xylan esterase activities. Native xylanase, Xyn10B.CB3B2 had carbohydrate binding modules (CBM), CBM3 and CBM2 in tandem at the C-terminus. CBMs are protein domains that bind carbohydrate ligands and are found in carbohydrate active enzymes. Truncation of CBM2 was done to create Xyn10B.CB3 while CBM3 was fused to N-terminus of catalytic domain to form Xyn10B.B3C. Fusion of CBM2 at the C- and N-termini of the catalytic domain resulted in Xyn10B.CB2 and Xyn10B.B2C, respectively. In addition, only the catalytic domain (Xyn10B.C) was also characterized in this study. All of the enzyme variants were successfully expressed in soluble fraction of Escherichia coli cells and purified through binding with regenerated amorphous cellulose except Xyn10B.C that was obtained as inclusion bodies and purified by refolding. Activities of Xyn10B.CB3B2, Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2, Xyn10B.B2C and Xyn10B.C on beechwood xylan were 118,305, 68,325, 65,825, 49,261, 44,518 and 40,368 U/μmol, respectively. Activities of Xyn10B.CB3B2, Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2, Xyn10B.B2C and Xyn10B.C towards p nitorphenylarabinofuranoside were 9,042, 4,532, 4,026, 5,672, 5,137 and 4,340 U/μmol, respectively. Activities of Xyn10B.CB3B2, Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2, Xyn10B.B2C and Xyn10B.C towards p-nitrophenylacetate were 15,545, 10,485, 8,856, 7,820, 7,571 and 7,342 U/μmol, respectively. All of the enzyme variants had optimum temperature 70 °C and optimum pH 6.0, under the vii assay conditions used. However, Xyn10B.C had optimum temperature and pH of 60 °C and 5.0-6.0, respectively. Binding assays revealed that all of the variants bound to insoluble oat spelt xylan and Avicel expect Xyn10B.C that did not bind to Avicel. Incubation of all enzyme variants with Mn2+ had negative impact on the activity of enzymes while other metal ions had no effect on the activity. Xyn10B.CB3B2 was stable up to 70 °C while Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2 and Xyn10B.B2C were stable up to 60 °C. Xyn10B.C was stable only up to 50 °C as thermal unfolding was observed beyond these temperatures during CD spectroscopy analysis. All of the enzyme variants were highly active producing xylobiose and xylose as end products, as well as debranching the substrates by removing arabinose and acetyl side chains as observed by HPLC analysis of the lysates and arabinose/acetate assays. This study successfully elucidated the characteristcs of a novel trifunctional xylanase, Xyn10B. Due to its specific characteristics, Xyn10B.CB3B2 and its variants seem to be of importance for industrial applications. In another study, XynI from Caldicellulosiruptor saccharolyticus DSM 8903 was expressed in E. coli as 35.8 kDa protein in soluble form, but the expression level was rather low. MFOLD analysis of the sequence between the ribosomal binding site and the 5¢-end codons of the gene showed that the start codon AUG was trapped in the mRNA secondary structure. Cloning the gene using pET28a(+) increased expression to a level of 35% as compared to about 4% when expressed using pET22b(+). pET28a(+), having His-tag before the start codon, would prevent strong secondary structure formation thus allowing higher expression level. Activity of XynI was found to be 10, 5 and 6 U/mg on beechwood, birchwood and oat spelt xylan, respectively. Further studies are required to elucidate the reasons behind low activity through molecular modelling and docking analyses.