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Assessment of Genetic Diversity of Indigenous Rice Germplasm from Northern Pakistan

Thesis Info

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Author

Bibi, Ayesha

Program

PhD

Institute

Hazara University

City

Mansehra

Province

KPK

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8839/1/Ayesha%20binding1.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725541240

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Rice (Oryza sativa L.) is one of oldest domesticated specie and a major staple food of around 3 billion people in the world. An investigation to find out the genetic diversity among 113 cold tolerant rice accessions acquired from plant genetic resource institute (PGRI) origionally obtained from Northern Areas of Pakistan along with three check varieties (Super Basmati, IR6, and JP5) was conducted. Morpho-physiological data was recorded for 19 qualitative and 19 quantitative traits at different growth stages during the summer season 2014 at two locations. The data was analyzed by cluster analysis and principal component analyses (PCA). Results revealed significant level of variability for all the morphological traits. Maximum variation was observed in grain yield plant-1, (433.92 g) and plant height (311.28 cm). The accessions were grouped into seven clusters based on Euclidean dissimilarity index. PCA based on 19 quantitative traits showed that first five PCs accounted for 67.86% of the total variance, PCI explained 28.41%, PCII contributed 13.38% and PCIII accounted for 11.69% of total variation. Polymorphic genetic markers were also used to identify species relationship and to asses the degree of genetic diversity in the accessions. On the basis of absence and presence of 18 visible polypeptides, an electrophoretic profile was established for construction of dendrogram through UPGMA method. A significant amount of variation was revealed in Glutelin and Prolamin subunits, while low variation was observed in Albumin and Globulin subunits. It can be inferred from the present investigation that lower genetic diversity was xiv present in rice accessions based on total seed storage protein profile SDS-PAGE alone was found to be insufficient for reliable differentiation. High yielding 96 accessions were selected through morphological and biochemical characterization for the determination of allelic variability through microsatellite analysis using 36 SSR markers. An average of 3.5 alleles per locus were observed. Polymorphism Information Content (PIC) value with an average of 0.638 per locus was observed. From the results it is concluded that SSR markers were effective in detecting polymorphism in the accessions studied and other crops with high genetic similarity. Bacterial leaf blight is one of the most serious diseases of rice in world. For molecular characterization of these accessions against bacterial blight and fragrance, tightly linked STS markers were used to amplify the target fragment. Accessions were also analyzed for presence or absence of bacterial blight resistant genes (Xa4, xa5, xa13, Xa21 and fgr) through PCR amplification using STS primers. Out of 96 accessions 42 accessions were found to have Xa4 resistant gene, while 50 accessions were found to have xa13 resistant gene. Fifty two accessions were found to have xa5 resistant gene which is most prevalent gene in Northern areas germplasm, Xa21 resistance gene and fgr gene were found to be absent in the germplasm studied.
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سلطان کھاروی دی گیت نگاری

سلطان کھاروی دی گیت نگاری

’’گیت ہندی زبان دالفظ اے جیدے معنی نیں راگ، سنگیت، بھجن ، سرور وغیرہ۔‘‘(۱)

ایس لئی گیت تے سنگیت دا آپو وچ گوہڑا سمندھ اے جتھے گیت اے اوتھے سنگیت اے تے جتھے سنگیت ہووے گا اوتھے گیت وی ہووے گا۔ گیت دراصل من دیاں ڈوھنگایاں چ جنم لین والے جذبیاں تے احساساں دے آپ مہارے پر گھٹاوے داناں اے ۔ جیہدے وچ سادگی، سلاست تے روانی مکمل درجے تک موجود ہوندی اے۔ ایسے پاروں آکھیا جاندا اے کہ گیت کوتا دا اک اجہیا روپ اے جو دلاں دیاں اتھا ڈوھنگایاں وچ پیدا ہوندی اے، تے دلاں دیاں اتھاہ ڈوھنگایاں تیک اثر کردی اے ۔ گیت کیول جذبیاں دے اظہار دا ناں ہی نہیں بلکہ ایہدے سرنانویاں وچ بڑی وسعت تے ون سونتا پائی جاندی اے۔نظم وانگوں گیت نوں وی خاص موضوع تیک محدود نہیں رکھیا گیاسگوں نظم جذبہ تے احساس دیاں نکی توں نکی کیفیتاں گیت دا موضوع بن سکدیاں نیں۔ ایس لئی عشق تے محبت ، کوشش تے محنت ، قومیت ، حریت رزم تے بزم ، کرم تے ظلم ، ہجر تے وصال ، جذبہ تے خیال، حسن تے جمال، عشق بے مثال ،ہراوہ شے جیڑی انسانی احساس دا حصہ اے گیت دا موضوع بن سکدی اے۔گیت بارے ڈاکٹر وزیر آغا لکھدے نیں:

’’گیت کا امتیازی وصف یہ ہے کہ ماں ، زمین یا معاشرے کے باطن میں پیدا ہونے والی کروٹ کا علم بردار ہے۔ اسی لیے گیت میں زمین سے وابستگی بہت توانا ہے۔ مثلاًگیت کی آواز میں دھرتی کی بہت سی دوسرے آوازیں شامل ہوجاتی ہیں۔ جسے کوئل کی کوک،مینا کا ترنم، بھنورے کی گھن گھن وغیرہ۔(۲)

سلطان کھاوری پنجابی زبان دے اچ کوٹی دے کوی نیں...

من خصائص النصانية القرآنية: النسخ

Since the “text” has received attention of all modern and post modern scholars and linguists, it has been changed. This “text” has different segments, some of them are applicable to Holy Qur’ān on the one hand, on the other hand there are some un-applicable too in connection of two textual boundaries of the “text”; bounded and un-bounded. From here many scholars declare that modern defined textual theory is totally un-applicable to Holy Qur’ān. Therefore, many Qur’ānic commentators and scholars refused “Intertextuality” with its all aspects. Anyhow some other scholars allow “Intertextuality” to be applied to Holy Qur’ān because of many Qur’ānic scholars have been interpreting this sacred text using the structural methods upon which contextual and intertextual phenomena and all the relevant conceptual components of this are based, this research intends elaboration of such dimensions of Qur’ānic “Intertextulaity” and “Textuality”, in particular, the Qur’ānic textulaity; “al-Nasḵ”; abolition, deletion and abrogation.

Influence of Packaging Materials and Storage Conditions on the Quality Attributes of Potato Solanum Tuberosum L. Tubers

Agro ecological diversity and favorable environment have enabled Pakistan to harvest bulk of potato crop however facing problems of poor post harvest management practices and unavailability of superior raw material for the potato processing industry. A comprehensive study was planned to identify best packaging material and appropriate storage conditions for the premium potato variety. The present study has been divided in to three different phase to address specific objectives. The first phase of study encompasses physico-chemical, functional and processing attributes in prominent potato varieties. The selected variety was subjected to different post harvest storage conditions along with their processing parameters analysis in second phase of the study. The last phase of study evaluated the storage stability of premium variety under best results identified in the second phase. In the first phase of study, physical attributes (tuber size, geometric mean diameter, sphericity, surface area, firmness, specific gravity, total soluble solids, pH, sprouting %, tuber color) Chemical attributes (dry matter, starch, protein, fat, sugar, fibre, ash and predominant minerals) functional attributes (ascorbic acids, glycoalkaloids, total phenolic contents, radical scavenging activity) and processing performance (chip moisture contents, fat absorption, color and sensorial attributes) were evaluated in ten commercial varieties i.e. Agria, Atlantic, Cardinal, Chipsona, Courage, Desi, Desiree, Hermes, Lady Rosetta, and Satellite. In general Lady Rosetta followed by Hermes was the most appreciable variety regarding their physical attributes. Lady Rosetta followed by Atlantic attained maximum dry matter and starch contents. Least sugar contents were recorded in Agria and maximum fat and protein contents were quantified in Desiree. In general; functional attributes were found maximum in Desi followed by Desiree. A promising correlation was estimated between most of these parameters with distinctive correlation (R=0.903) identified between total phenolic contents and radical scavenging activity. Post processing parameters like moisture contents, fat absorption, and sensory evaluation in Lady Rosetta showed its preference over all other varieties followed by Hermes. In the second phase variety” Lady Rosetta” was evaluated under different storage conditions 1 2 (packaging, light, temperature, and anti sprouting agents) on the basis of transition in their quality attributes (reported in 1st phase) and enzymatic (polyphenol oxidase, peroxidase) activities. Potatoes were stored under different packaging materials (jute, nylon, polypropylene, cotton, low density polyethylene, medium density polyethylene, high density polyethylene) at ambient temperature (25±2oC). Results revealed that polypropylene packaging and low density polyethylene packaging were found best and maintained tubers quality attributes up to 63 days, while other packaging materials were also found effective as compare to control. Thirty days storage of tubers under different illuminations (blue, fluorescent, green, mercury, red, dark) at ambient temperature (25 ±2oC) was carried out. Potato tubers kept under dark presented minimum loss of quality parameters however green and mercury lights posed best storage performance over all other illuminations during one month. Tubers were found highly susceptible to fluorescent light with poor processing attributes were recorded in red and blue light exposures. Results under comparative temperature regimes (5, 15 and 25oC) showed maximum storage stability up to 126 days under 5oC, however associated with low enzyme activity, elevated sugars contents in tubers and poor processing performances in fried chips. Exposing tubers to different anti sprouting agents (hot water treatment, spearmint oil, clove oil, CIPC) showed that CIPC and Clove oils applications were found significant in preventing tuber sprouting at the end of 80 days storage. In general, both retained superior tuber characters with remarkable processing characters during the storage period. Tuber dormancy was ensured under both treatments till the end at ambient temperature (25 ± 2oC) storage. In the last phase integrated post harvest management of potato variety “Lady Rosetta” on the basis of best results identified in second phase ensured tuber dormancy and prolonged storage life up to 180 days with appreciable retention of tuber quality attributes and superior processing performance as compare to 100% sprouting observed in control on the 80th day of storage. Coating of potato chips with 20% aloe vera gel presented best results with reduced fat uptake along with appreciable sensorial scores.