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Association Mapping for Economically Important Traits in Brassica Juncea

Thesis Info

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Author

Ishaq Ibrahim, Muhammad

Program

PhD

Institute

Hazara University

City

Mansehra

Province

KPK

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Genetics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11106/1/Muhammad_Ishaq_Ibrahim_Genetics_HSR_2019_HU_1.08.2019.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725550953

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The study was launched to estimate the genetic diversity and association mapping amongst Brassica juncea genotypes through agro-morphological traits. To estimate the genetic diversity data was recorded on 15 qualitative and 17 quantitative traits of 331 genotypes for two successive years. The recorded data was analysed through cluster and PCA. Valuable genetic variability was found for no. of days to physiological maturity, days to flowering and plant height. Pod shattering percentage have medium level of variation. Among the studied traits, highest and significant correlation (.97**) was recorded for days. to. flowering. initiation. and 50% days to flowering, flower initiation days and flowering completion days and days to half flowering and flowering completion days followed by correlation (0.89**) between flower initiation days and no. of days to physiological maturity. The genotypes studied were divided into 4 main groups based on cluster analysis. It was found that 6 out of 17 principal components with an eigenvalue of ≥ 1 accounted for 68.58% difference of the overall diversity found among the studied genotypes. The contribution of first 3 PCs in overall diversity was 30.63%, 10.30%, 8.92% respectively. For getting promising results, the elite genotypes could be recommended for future breeding programme. The association study exhibits identification of markers associated with economically important traits. A total of 131 B. juncea L. genotypes were used in the GWAS. Illumina® 90K Brassica Infinium Assay (~90,000 SNPs) were run on selected genotypes. Multiple SNPs distributed on most of the chromosomes were identified. A total of 366 significant loci (-log10 P-value ≤0.001)were identified for 17 economically important traits of Brassica juncea L. genotypes including, days to half flowering(DF50%),flowering completion days(DFC),no. of days to physiological maturity (DM), Pod shattering percentage (PS), Leaf length (LL), Leaf width (LW), Plant height (PH), No. of primary branches per plant (PB/P), Length of main raceme(MRL), Pods on main raceme(P/MR), Stem thickness (ST), Pod length (PL), Pod width (PW),no. of grains per pod (G/P),grain yield( GY/P), thousand seed weight ( TGW) and Blackleg in total of 77,969 SNPs with minor allele frequency (MAF) ≥ 0.05. 6, 6, 4, 8, 9, 8, 1, 1, 4, 6, 4, 4, 1, 10, 6 and 3 SNPs were identified on multiple chromosome locations for DF50, DFC, DM, LL, LW, PH, PB/B, PL, PW, Pod Shattering, ST, P/MR, S/P,GY/P,TGW and Blackleg, respectively. The discovered major SNPs could help breeders to select high performing Brassica juncea L. genotypes through marker assisted selection (MAS) as well as to develop elite lines for future breeding program.
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پروفیسر عبدالمعید خاں

پروفیسر عبدالمعید خاں
افسوس ہے گزشتہ ماہ کی ۲۵/تاریخ کوپروفیسر عبدالمعید خاں کابھی انتقال ہوگیا۔ مرحوم برصغیر کے اساتذہ ٔ عربی واسلامیات میں ایک نمایاں مقام رکھتے تھے۔ انھوں نے پہلے قاہرہ میں کئی برس مقیم رہ کر وہاں سے ڈی۔لٹ کی ڈگری لی اور پھر کیمبرج سے پی۔ایچ ڈی کیا۔اس کے بعد جامعہ عثمانیہ سے وابستہ ہوئے تو ایسے کہ ساری زندگی یہیں بیت دی۔ابھی دوتین برس ہوئے وہ صدر شعبۂ عربی کے عہدہ سے سبکدوش ہوئے تھے۔ ۳۵برس سے اسلامیات کے مشہور اوربلند پایہ سہ ماہی رسالہ’’اسلامک کلچر‘‘کے ایڈیٹر اوربارہ برس سے دائرۃ المعارف کے ناظم تھے۔ اس درمیان میں امریکہ اور یورپ کے جامعات میں وزیٹنگ پروفیسر کی حیثیت سے بھی کام کرتے رہے۔ بیرونی ممالک کے بعض سفروں میں راقم الحروف اوروہ دونوں ہم سفر تھے۔اخلاق وعادات کے اعتبار سے بہت سادہ اورمنکسر المزاج تھے۔ اگرچہ انھوں نے بہت کم لکھاہے اوراس کی وجہ یہ ہے کہ وہ شروع سے ہی خرابی صحت کاشکار تھے اوراسی سبب سے ڈاکٹروں کی ہدایت کے مطابق عمر بھرمجرد رہے، لیکن جوکچھ لکھاہے بہت سخت لکھاہے۔ عمر۶۶برس کے لگ بھگ ہوگی۔ عقیدہ اورعمل کے لحاظ سے پکے اورسچے مسلمان تھے۔ اﷲ تعالیٰ ان کو مغفرت کی نعمت سے سرفراز فرمائے۔
[اکتوبر۱۹۷۳ء]

 

Ijaz Hussain, Political and Legal Dimensions of Indus Water Treaty (Book Reviews)

Of the many issues that the partition of India in 1947 did not address in time, and were left to worsen the relations between India and Pakistan, the issue of riparian rights was only next in importance to the issue of Kashmir. In many respects the two issues have direct bearing on each other as well, as some important rivers emanate from Indian-held Kashmir. As the time passes more and more historians are coming to the conclusion that the massacre at the time of partition, the uprooting of more than 12 million people from their native homes, horrendous violence against women, burning and looting, etc, all owe mainly to the colonial administration that failed to ensure peaceful transition to two independent countries. No less than a person than a former Prime Minister of Great Britain, Winston Churchill, described the attitude of the then British government as the ‘biggest escape of human history’. In its urge to get rid of Indian responsibility, the government of Prime Minister Clement Atlee, showed extraordinary haste in relinquishing its responsibilities without ascertaining that the partition of a country of a subcontinental size would involve a huge amount of work. It required taking into consideration all administrative and security aspects so that the two countries could move along their independent journey as good neighbours. Unfortunately, the failure of the British government, both in London and, through its viceroy, in Delhi, left a number of issues unsettled, which the two independent countries have been trying to resolve for the last seven decades. Moreover, with the passage of time, new factors emerged which further complicated the original issues.

Association Between Female Sexual Dysfunction and Fertility Among Women of Reproductive Age at the Aga Khan University Hospital, Nairobi.

Introduction: Sexual function plays an essential role in the bio-psychosocial wellbeing and quality of life of women and disturbances in sexual functioning often result in significant stress. Sexual dysfunction in women is a highly prevalent condition affecting up to 43% of women. Subfertility being a common problem affecting up to 20% of the population, causes significant psychosocial distress which might adversely affect sexual functioning and vice versa. However, despite the high prevalence of both conditions, little has been studied on the effects of subfertility on sexual functioning especially in sub-Saharan Africa. Objectives: This study primarily compared the prevalence of female sexual dysfunction in patients on assessment for subfertility and those seeking fertility control services at the Aga Khan University Hospital, Nairobi. We secondarily sought to determine the factors associated with female sexual dysfunction in the sub-fertile patients and those seeking fertility control services. Methods: This was an analytical cross sectional study. Eligible women of reproductive age (15-49 years), attending the gynaecological clinics with complaints of subfertility and those seeking fertility control services were requested to fill a general demographic tool containing personal data and the Female Sexual Function Index (FSFI) questionnaire after informed consent. Prevalence of sexual dysfunction was calculated as a percentage of patients not achieving an overall FSFI score of 26.55. Univariate and multivariate analysis were done to compare clinical variables to delineate the potential association. Results: The prevalence of female sexual dysfunction was 31.2% in the subfertile group and 22.6% in fertility control group. The difference was not statistically significant (p=0.187). The mean domain and overall female sexual function scores were lower in the subfertile group than the fertility control group though this was not statistically significant. The most prevalent sexual domain dysfunctions in both the subfertility and fertility control groups were desire and arousal while the least in both groups was satisfaction dysfunction. Subfertility type was not associated with sexual dysfunction. Higher education attainment was protective of female sexual dysfunction in the subfertile group while use of hormonal contraception was associated with greater sexual impairment in the fertility control group. Higher maternal age and alcohol use appeared to be protective against sexual dysfunction in the combined study population. Conclusion: The present study demonstrated no association between the fertility status and the prevalence female sexual dysfunction. Subfertility type was not associated with sexual dysfunction. Education level and hormonal contraception use were associated with female sexual dysfunction in