Type 2 diabetes mellitus (T2DM) is an increasingly common metabolic disorder with a substantial inherited component. The inheritance pattern is complex and polymorphisms of several genes might influence genetic susceptibility of the disease that is characterized by islet dysfunction and insulin resistance. Although various characteristics of diabetes mellitus in local population have been investigated, progress in defining genetic factors is meager. As the genetic architecture of T2DM may vary between diverse ethnic populations, it is critical that such variants are examined in Pakistani population. The present project was aimed to investigate association of vitamin D receptor(VDR) gene polymorphisms with T2DM in Pakistan. Methodolgy included documentation of demographic charateristics and comparative analysis of biochemical parameters (glucose, HbA1c, vitamin D, lipid profile, liver function tests and renal function tests)in diabetic and normal participants. Genomic DNA was used for genotyping of four restriction fragment length polymorphism (RFLP) sites; BsmI, ApaI, TaqI and FokI by polymerase chain reaction (PCR) amplifications and restriction endonuclease digestion of the products. The digested PCR products were separated on agarose gel electrophoresis. Among all the demographic parameters, systolic and diastolic blood pressure and BMI (body mass index) were significantly higher (p<0.001) in diabetic group as compared to the control group. Hyperglycemia, renal and lipid profiles were significantly inversely associated (p<0.01) to vitamin D levels in T2DM subjects.Differences of FokI, BsmI and TaqI genotypes of VDR gene were significant between T2DM and normal groups (p<0.01).While ApaI showed non-significant association to the T2DM in local population. No significant association was found between biochemical parameters and all four restriction sites (ApaI, BsmI, FokI and TaqI) (p>0.01). In addition, VDR gene polymorphisms were related non-significantly (p>0.05) to the diabetic complications in the present study. To conclude, VDR gene polymorphisms (BsmI, FokI and TaqI) may contribute to the onset and progression of T2DM in local Pakistani population but association between VDR genetic polymorphisms to various diabetic complications is still not clear and warrants additional functional genomics studies to verify the genetic susceptibility of VDR gene to T2DM onset and progress.
مراتب اخترنے ساٹھ اور ستر کی دہائی میں اپنے منفرد ڈِکشن کی بنا پر شہرت حاصل کی۔ انھیں مجیدامجد کی صحبت سے فیض یاب ہونے کا موقع بھی مِلا۔ مجیدامجد سے اُن کی ملاقاتیں بھی اُن کے جدید شعری رجحان کا سبب بنیں۔ مجیدامجد مراتب اختر کے بارے میں کہتے ہیں:
یہ ایک شاعر ہیں جو غزل کہتے ہیں اور غزل ان کے عقیدئہ حیات کا ایک جزو ہے۔ یہ عقیدہ ان کی رُوح کے لیے شرط ایمان ہے، کوئی عجب شیفتگی ہے جو انھیں اس صنف کے ساتھ ہے۔ ایک عمر سے وہ غزل کے مصنوعی انداز کو نکھارنے میں مصروف ہیں۔ یہاں ان اشعار کے اندر ایک بالکل نیا چہرہ مفاہیم ہے۔ لذتِ بیان کی ایک انوکھی سرشاری ہے۔ بظاہر ایک سہمی ہوئی آواز ہے لیکن دراصل یہ اپنی ہی توانائی سے شرمائی ہوئی آواز ہے۔ نئے اِمکانات اظہار ہیں، نیا جلوئہ حروف ہے۔ ایسا معلوم ہوتا ہے گویا اظہار کے پردے میں شاعر اپنے آپ ہی سے مخاطب ہے۔ خود ہی اپنی آواز، خود ہی اس کا سننے والا اور خود ہی اس سے کیفیت گیر ہے۔ ان اشعار پر مکاشفوں کا گمان ہوتا ہے۔ اپنے تاثر پر اپنا اعتقاد، اپنے اعتقاد پر اپنا ایمان، اپنے اسی اطمینان کا وقار، ان کے ہر شعر سے جھلکتا ہے۔ جابجا ایک ضبط ہے جس کی اپنی تمکنت ہے۔ ایک شکستگی ہے جس کا اپنا جلال ہے۔ ایک کرب مہجوری ہے، جس میں گراوٹ نہیں متانت ہے۔ ایسا احساس ہوتا ہے جیسے محبوب کے ساتھ بات کرتے وقت شاعر کے لہجے میں محبوب کا انداز رضامندی اس میں شامِل ہو گیا ہے۔۔۔ جہاں خارجی اشیاء کا بیان ہے، وہاں یوں لگتا ہے جیسے یہ اشیاء اپنا ٹھوس وجود...
Islam is a religion of humanity; it takes much care of people and worries a lot about both the spiritual and the materialistic aspect of life. That’s way it gives a clear and solid code of life which don’t leave blank any single aspect of human life. Spiritual training and education is a major part of Islamic teachings, Islamic training goes side by side the Islamic education as both tied strongly to each other’s and there is no option to disconnect the Islamic education from the Islamic training and practice. Islam stresses on both the education and the training aspects at a time and don’t believe in dualism at all in Muslim society about Islam and its teachings, one who does not practices what he learns in training is much worse than one who don’t practices due to his ignorance. This verse of S┴ra Jum’ ā describes the importance of Islamic Training in a very clear way as this verse declares the Islamic training as a basic reason of sending the Prophet PBUH to the humanity.
The importance of linguistic variation has been discussed in a variety of theoretical, methodological and practical studies (e.g. Biber, 1988, 1994, 2002, 2009, and 2013). These studies have contributed to the description of any language variety defined by its situational characteristics. Pakistani English (PE) is an emerging independent, non-native variety of English. However, currently there is a lack of a comprehensive description of its characteristics in the literature. The present research addresses this problem by investigating the linguistic variation of Pakistani English newspaper editorials (PNE.). It aims to identify if the language used in PNE is a distinguished linguistic sub-register of Pakistani Newspaper English. In order to address this question, the technique of multidimensional (MD) analysis is utilized, which provides detailed grammatical information about the corpus and helps in interpreting and comparing the corpus to the text genres and typologies that have already been studied, labeled and commonly acknowledged in the English language. A diverse dataset, consisting of 1500 editorials from five leading newspapers, with three subcategories (personal editorials, organizational editorials and letters to the editor) has been compiled which provides a variety and range of topics covered by different authors of the Pakistani community. The resulting corpus is tagged for 147 linguistic features and factor analysis has been conducted, to identify major linguistic patterns of co-occurrence. Total nine textual dimensions are utilized to define some of the linguistic and functional characteristics of PNE corpus. The dimensions collectively provide information content, narrative features, situation-oriented references, extent of argumentative language, abstract style, evidencebased discourse, specialized information and interactive discourse. The results of the old (previously identified dimensions; 88 MD analyses) and new MD (Full MD analysis to determine new dimensions) shows marked linguistic variation among different categories of the PNE corpus. Based on these results, it is proposed that linguistic variation occurs in the editorial writing of Pakistani English on both national and international levels.