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Azo Dyes Removal by Bacteria Isolated from Industrial Wastewater

Thesis Info

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Author

Mehvish Ajaz

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Microbiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12399/1/Mehvish%20ajaz%20microbiology%202019%20uop%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725563569

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In the present study, Alishewanella sp. CBL-2 was characterized on the basis of biochemical and 16S rRNA sequencing. The bacterium was found to decolorize 83% Sumifex Tourqi blue within a period of 6 days at 37C and pH 7. The predominant form of the enzyme was found to be extracellular (78%) as compared to intracellular (16%). The breakdown of azo bond was confirmed through thin layer chromatography (TLC) analysis as new bands and peaks were observed in chromatograms of extracted metabolites of decolorized samples. Growth of beneficial microbial fauna in the presence of bacterial decolorized wastewater indicates that this wastewater is safe for the survival of microorganisms. High bioremediation potential makes Alishewanella sp. CBL-2 an impending foundation for green chemistry to remove azo dyes from industrial wastewater.In the present study, Alishewanella sp. CBL-2 was characterized on the basis of biochemical and 16S rRNA sequencing. The bacterium was found to decolorize 83% Sumifex Tourqi blue within a period of 6 days at 37C and pH 7. The predominant form of the enzyme was found to be extracellular (78%) as compared to intracellular (16%). The breakdown of azo bond was confirmed through thin layer chromatography (TLC) analysis as new bands and peaks were observed in chromatograms of extracted metabolites of decolorized samples. Growth of beneficial microbial fauna in the presence of bacterial decolorized wastewater indicates that this wastewater is safe for the survival of microorganisms. High bioremediation potential makes Alishewanella sp. CBL-2 an impending foundation for green chemistry to remove azo dyes from industrial wastewater.
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