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Home > Bacterial Diversity in the Root Nodules and Rhizosphere of Chickpea Cicer Arietinum L.

Bacterial Diversity in the Root Nodules and Rhizosphere of Chickpea Cicer Arietinum L.

Thesis Info

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Author

Zaheer, Ahmad

Program

PhD

Institute

Pakistan Institute of Engineering and Applied Sciences

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7917/1/A%20Zaheer%20Thesis%2020%20july2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725566128

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The main objective of the present study was to study bacterial diversity in the root nodules and rhizosphere of chickpea varieties growing in different regions of Pakistan by cultivation on growth media as well as by using culture-independent DNA-based techniques. A total of 60 isolates, including symbiotic (10 isolates) as well as free-living bacteria (50 isolates), were purified from “Desi-type” and “Kabuli-type” chickpea varieties collected from 5 different localities. In pure culture, maximum IAA production was recorded in Kocuria sp. RTL99 (37.77 μg/mL) and maximum phosphate solubilization was recorded in Serratia sp. 5D (119.94 μg/mL). Among the bacterial inocula tested in pot and field experiments, co-inoculation of Mesorhizobium sp. NTY7 and Ensifer sp. NFY8 was found to be the most effective treatment at all localities and on both varieties of chickpea. To investigate bacterial diversity by culture-independent DNA-based technique, DNA extracted from root nodules and rhizospheric soil was used for pyrosequencing of 16S rRNA and nifH genes. 16S rRNA sequences originating from the nodules revealed occurrence of 10 bacterial phyla. At genus level, 16S rRNA sequences of 111 genera (70.78 % of the total sequences) of culturable bacteria were retrieved from nodule DNA along with 29.22 % sequences of “uncultured” bacteria. In the nodules, a significant fraction i.e., 52.77 % of 16S rRNA sequences and 88.83 % of the nifH sequences among the total sequences retrieved from all sites belonged to genus Mesorhizobium. The 16S rRNA sequences originating from the rhizospheric soil revealed enormous diversity of 22 bacterial phyla. At genus level, 16S rRNA sequences of 313 genera (29.72 % of the total sequences) of culturable bacteria were retrieved from rhizospheric soil DNA along with 70.28 % sequences of “uncultured” bacteria. Mesorhizobial 16S rRNA and nifH sequences retrieved from rhizospheric soil comprised 0.265 % and 16.68 % of the total recovered sequences, respectively. In the present study, sequences related to well-known plant growth promoting rhizobacteria Serratia spp. were frequently detected, which lead to targeted isolation of two Serratia strains from the nodules. Both the isolates showed growth improvement of chickpea when used as inoculants for chickpea grown at different localities.
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شیخ انس یاسین

شیخ انس یٰسین
دوسرا حادثہ سعودی عرب کے سابق ہندوستانی سفیر شیخ انس یٰسین کی وفات کا ہے، ابھی وہ جوان تھے، لیکن تدبر و معاملہ فہمی میں تجربہ کار بوڑھوں سے کم نہ تھے، وہ مختلف اوقات میں مختلف ملکوں کی سفارت پر رہے، آج کل ٹرکی میں سفیر تھے، وہیں کار کے حادثہ میں وفات پائی، ان میں اپنے مذہب و ملت کا بڑا درد تھا، ہندوستان کے اسلامی اداروں سے ان کو خاص دلچسپی تھی، اپنی سفارت کے زمانہ میں متعدد اداروں کو دیکھا اور ان کی مدد بھی کی، دارالمصنفین کے بھی محسن تھے، یہاں آنے کا وعدہ بھی کیا تھا، مگر ایسے موقع پیش آتے رہے کہ آنا نہ ہوسکا، دو سال ہوئے دارالعلوم ندوۃ العلماء کے مدرسہ ثانویہ کی عمارت کا سنگ بنیاد رکھنے کے لئے لکھنو آئے تھے تو ان سے ملاقات ہوئی تھی، اﷲ تعالیٰ ان کی مغفرت فرمائے۔ (شاہ معین الدین ندوی،اگست ۱۹۷۴ء)

Accounting Guidelines Implementation: A Study on Economic Development of Islamic Boarding Schools

The development of Islamic boarding schools is one of the targets for sharia economic development which is based on the understanding of the halal value chain where the development of Islamic economics and finance summarizes various initiative programs including the development of Islamic boarding schools. The purpose of this study is to describe how the implementation of the Islamic Boarding School Accounting Guidelines at Al Huda Islamic Boarding School which is more focused on the readiness of HR at Al Huda Islamic Boarding School in applying the Islamic Boarding School Accounting Guidelines. The Al Huda Islamic boarding school was chosen as the research locus because the Al Huda Islamic Boarding School was appointed by Bank Indonesia Gorontalo Representative Office as a pilot project for the Pesantren (Islamic Boarding School) which applies the Islamic Boarding School Accounting Guidelines. The stages of the research method began with observations and results of interviews with informants from the board of the Al-Huda Islamic Boarding School. The results showed that there was a desire to apply these guidelines in the Al-Huda Islamic boarding school. Even though treasurers, secretaries and operators do not have a basic knowledge of the Economics of Accounting, the existing human resources are ready to apply the Pesantren accounting guidelines, of course, adjusting to the needs of the Al-Huda Islamic boarding school. The author sees that there is no urgency in implementing Islamic boarding school accounting guidelines at the Al-Huda Islamic boarding school because the reporting entity is the foundation that houses the Al-Huda Islamic boarding school, the Al Huda Islamic Education and Da'wah Foundation, Gorontalo. The foundation is fully responsible for the donors or the community.

Effect of Sewage Slude and Compost Application on Soil Health and Plant Growth

Application of sewage sludge and solid waste compost to crop land is considered as the best option for their safe disposal. However, presence of heavy metals at high levels in such amendments warrants their characterization in order to assess their impacts on soil and plant health. This is particularly relevant for developing countries like Pakistan, where limited information on quality and/or composition of such amendments is available. Therefore, studies in laboratory, greenhouse and filed conditions were planned. The six sewage sludge samples collected from various sources were, (1) Water And Sanitation Agency waste water treatment plant Faisalabad (WSS), (2) I-9 sector wastewater treatment plant Islamabad (ISS), (3) Kasur sewage sludge (KSS), (4) Coca-Cola sewage sludge (CSS), (5) National University of Science and Technology sewage sludge (NTS), (6) Nestle company sewage sludge (NSS) and seven compost samples collected were (1) Green Force Fertilizer (GCC), (2) Super Bloom company (SBC), (3) Lahore compost company (LCC), (4) University of Agriculture, Faisalabad (UAC), (5) Uni-Grow Bio fertilizer (BFC), (6) N.K Agro chemicals (NAC) and (7) Estate care management compost, University of Agriculture, Faisalabad (ECC). The collected samples were analyzed for macronutrients, micronutrients heavy metal/oids and some physicochemical properties. The sewage sludge and compost samples showed neutral to slightly alkaline pH. The sewage sludge samples showed higher salt content as compared to composts with the highest value of 12.06 dS m-1 in WSS. The compost samples contained more concentration of macro and micronutrients compared to sewage sludge samples with the highest value of SBC and UAC. The concentrations of micronutrients varied a lot, SBC and LCC showed the highest levels of boron (1.15 mg kg-1) and copper (5.08 mg kg-1). On an average, the sewage sludge samples contained higher contents of heavy metals as compared to composts except LCC which had more contents of cadmium (Cd) and lead (Pb). The highest concentration of arsenic (As), chromium (Cr) and Pb were found in CSS, KSS and ISS, respectively. On the basis of characterization study, another trial was undertaken in glass settings using pots without leaching provision. In this study, impact of selected composts and sewage sludges on growth and yield performance of rice and subsequent wheat crop and levels of various heavy metals in soil solution and plant tissues were evaluated. Four types each of compost and sewage sludge were selected from first study and soil applied at 0.5% (w/w) and 1.0% (w/w). Control pots for treatment comparison received recommended dose of fertilizer only (RDF, no sewage sludge/compost). All these treatments were tested in two types of soils which differed in their texture. The plant physiological attributes like stomatal conductance, transpiration rate, photosynthetic rate and chlorophyll contents were negatively affected by the concentration of heavy metals in plant parts (rice and wheat). Among composts, the LCC increased the concentration of heavy metals (Cd and Pb) in rice and wheat plant tissues grown on both soils at harvest. The Cd concentration in rice grains with LCC, ISS, KSS and CSS applied at 1.0% was above the Indian safe limit (1.5 mg kg-1) in both types of soils. The concentration of dissolved organic carbon (DOC) in soil solution decreased with time during both rice and wheat crops and it showed a linear relationship with heavy metals in plant tissues in both type of soils. Composts performed better in terms of growth and yield and metal accumulation in grains of both crops remained lower than sewage sludges except LCC in both soils which showed higher levels of Cd and Pb in plant parts compared to control. Among soils, the plants grown on clay loam soil (Soil-I) showed higher yield and less heavy metal accumulation as compared to plants grown on sandy clay loam (Soil-II). Irrespective of amendment type, amendment addition at 0.5 % produced higher grain yield and lower metals accumulation in both the crops and both type of soils. Similarly, irrespective of amendment type, their application at 1.0% improved grain yield of both the crops only in Soil I, whereas the grain yield of both the crops declined at this level with LCC, WSS, ISS, KSS in Soil-II. A field experiment was conducted to evaluate the best application level among selected sewage sludges and composts samples by growing rice and wheat as test crops. Maximum straw and grain yields of both the crops were recorded with SBC at 0.5% in both type of soils. The lowest rice and wheat grain yield in Soil-I recorded with LCC and control treatment, respectively. In case of Soil-II, the lowest rice and wheat grain yields were recorded with WSS at 0.25% and 0.5% level, respectively. Overall, the plant grown in Soil-I showed higher grain and straw yields at each level of treatments as compared to Soil-II. However, the application rate of 0.5% is not recommended to avoid the possible risk of metals accumulation in soil and plants. At this rate, however, yield is to low but the possible risks of metal uptake and accumulation in the soil-plant system is less. The SBC at both the levels (0.25% and 0.50%) of application remained safer for rice and wheat plants as well as for soil health.