Home > Bioactive Principals from Teucrium Royleanum Wall. Ex Benth. and Perovskia Atriplicifolia Benth. - Antimicrobial, Allelopathy and Antioxidant Assays
Bioactive Principals from Teucrium Royleanum Wall. Ex Benth. and Perovskia Atriplicifolia Benth. - Antimicrobial, Allelopathy and Antioxidant Assays
This dissertation describes the essential oil analysis, antioxidant, antimicrobial and allelopathy of two plants belonging to the genus Teucrium and Perovskia namely, Teucrium royleanum Wall. ex Benth., and Perovskia atriplicifolia Benth. belonging to the family Lamiaceae. The Teucrium royleanum was also subjected to the isolation and characterization of the secondary metabolites. Investigation of T. royleanum resulted in isolation of three (3) new compounds, the skeletons of two belonging to terpenes, named as royleanumin 1, and royleanumioside 2, while the third was named as royleanumoate 3. The compounds 1 and 2 were subjected to allelopathy assays against lettuce seedlings (weed). Compound 1 exhibited remarkable phytotoxic activities and could prove as a lead compound for the development of environmentally friendly weedicides. The known compounds isolated from this plant included, 5-hydroxy-4,7-dimethoxy flavone 4, 5,7-dihydroxy-3,4-dimethoxyflavone 5,4′, 6-dihydroxy 5,7-dimethoxyflavone 6,3,4-dihydroxymethylbenzoate 7, oleanolic acid 8, β-sitosterol 9 and β-sitosterol glucoside 10. The literature survey reveals that flavonoids are good allelochemicals and thus the characterized 5-hydroxy-4,7-dimethoxyflavone 4, 5,7-dihydroxy-3,4-dimethoxyflavone 5, 4′,6-dihydroxy 5,7-dimethoxyflavone 6 were subjected to the allelopathy assays. All compounds exhibited strong allelopathy activities to the test lettuce seedlings. Consequently, these compounds can also be used as lead structures for studies on the development of new synthetic herbicides. iiAnalysis of the essential oil obtained from Teucrium royleanum Wall. ex Benth. by means of GC-MS resulted in qualitative and quantitative identification of forty six known compounds comprising of sesquiterpene hydrocarbons (42.2 %), oxygenated sesquiterpenes (21 %), monoterpene hydrocarbons (17.3 %), oxygenated monoterpenes (7.7 %). The antioxidant capacity of the essential oil was evaluated by means of 1, 1-Diphenyl-2-Picryl-Hydrazyl (DPPH) and superoxide anion methods. The same essential oil was also subjected to allelopathy assays and showed promising results. Teucrium royleanum was tested by four variant assays in a separate set of experiments using lettuce seeds, showing excellent inhibition of the growth of lettuce seedlings. The plants leaf leachates were assayed by Sandwich and Homogenated Sandwich methods while Dish Pack Method was used for the evaluation of essential oils. The above stated methods confirmed the presence of allelochemicals in T. royleanum and were further evaluated in bioassay guided way by total activity approach. The crude methanolic extract fractionated with n-hexane, chloroform, ethylacetate, butanol and water fractions were assayed by the Total Activity Method. The essential oils, n-hexane and chloroform fractions played a significant role in our findings. The results from this study suggested that the secondary metabolites from Teucrium royleanum can be potential candidates for the phytotoxicity (Allelopathy). Analysis of the essential oil obtained from Perovskia atriplicifolia Benth. by means of GC-MS resulted in the qualitative and quantitative identification of eighteen compounds. The major constituents were camphor (28.91%), limonene (16.72%), ά-globulol (10.21%), trans-caryophyllene (9.30%), and ά-humulene (9.25%). Antimicrobial activity of the oil was evaluated using Agar Diffusion Method and Agar Dilution Method. The antimicrobial test results showed that the oil possesses significant antimicrobial activity iiiagainst ten bacteria. The bacterial strains tested were Bacillus cereus, Bacillus cerus subsp. mycoides, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecium, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Klebsiella pneumoniae (clinical isolate), and Yersinia enterocolitica respectively and five fungal strains namely Candida albicans, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Aspergillus parasiticus, and Geotricum candidum (wild type). The over all results of our studies on the essential oil of P. atriplacifolia suggests it to be a good source of antimutagenic and antimicrobial agents and thus can prove as a good candidate for utilization in the cosmetics, food and pharmaceutical industries.
مولانا عبدالسلام خاں رام پوری اخباروں سے یہ افسوس ناک خبر ملی کہ ۱۳؍ اپریل کو مولانا عبدالسلام خاں رام پوری نے اس دنیائے فانی کو الوداع کہہ دیا، اِناﷲ وَاِنا اِلَیہ رَاجِعُون۔ ان کے ساتھ ہی دارالسرور رام پور کی وہ امتیازی شناخت بھی رخصت ہوگئی جس کی وجہ سے رام پور کو بخارائے ہندی کہا جاتا تھا، رام پور کی ریاست کی علم پروری اور سخن نوازی کی داستانیں ہماری علمی و ادبی تاریخ کا بڑا دل کش حصہ ہیں لیکن فلسفہ و کلام و منطق جیسے علوم معقولات میں اس ریاست کی روایت کی بات ہی کچھ اور ہے، اٹھارہویں صدی کے اواخر میں نواب فیض اﷲ خاں کے فیض سے جب وہاں مدرسہ عالیہ قائم ہوا اور اس کے پہلے صدر مدرس کی حیثیت سے مولانا عبدالعلی بحرالعلوم فرنگی محلی کا تقرر ہوا تو جیسے معقولات کی بہار آگئی، مولانا فضل حق خیرآبادی اور مولانا عبدالحق خیرآبادی جیسے ائمہ فلسفہ اسی فصل گل کی یادگار ہوئے، مولانا عبدالسلام خاں نے جب رام پور کی اس فضا میں ۱۹۱۷ء میں پہلی سانس لی تو گو پہلا سا رنگ نہیں تھا لیکن رونق اب بھی باقی تھی، ان کے ہم عصر ساتھیوں میں مولانا وجیہ الدین خاں، مولانا ابوالوفاء شاہ جہاں پوری، مولوی عبدالوہاب خاں، مولانا امتیاز علی عرشی جیسے اصحاب فضل و کمال کے نام ملتے ہیں، ان کے اساتذہ میں ایک نام جیراج پور اعظم گڑھ کے مولوی عبدالودود ندوی کا بھی ہے، مولانا عبدالسلام خاں کی غیر معمولی لیاقت ہی تھی کہ ان کو کم عمری میں اس مدرسہ عالیہ کا متولی یعنی پرنسپل بنایا گیا اور یہ ان کی صلاحیت تھی کہ وہ ۱۹۷۵ء تک یعنی قریب تیس سال تک اس عہدے پر فائز رہے لیکن ان کی اصل شہرت ان کے قلم کی رہین منت ہے جس نے...
هدفت هذه الدراسة إلى التعرّف على علاقة التفكير الإستراتيجي بالأداء الوظيفي في وزارة التربية والتعليم الفلسطينية ومديريّاتها، إضافة إلى التعرّف إلى واقع ممارسة أبعاد التفكير الإستراتيجي في الوزارة، ومعرّفة مستوى الأداء الوظيفي في الوزارة، كما تمثلت مشكلة الدراسة في ندرة الدراسات والبحوث التطبيقية التي تتناول التفكير الإستراتيجي ومستوى الاهتمام به وعلاقته بالمتغيرات الأخرى، خاصة في القطاع العام في فلسطين، ولتحقيق أهداف الدراسة استخدم المنهج الوصفي التحليلي، واستخدمت الإستبانة أداة لجمع البيانات اللازمة من مجتمع الدراسة وعدده 550 موظفًا، وقد اعتمدت العينة العشوائية الطبقية النسبية ، وقد توصلت الدراسة إلى مجموعة من النتائج أهمّها أنّ درجة تطبيق موظفي الإدارة العليا في وزارة التربية والتعليم للتفكير الإستراتيجي متوسطة، حيث بلغت نسبة تطبيق موظفي الإدارة العليا للتفكير الإستراتيجي (61.40%)، وأنّ مستوى الأداء الوظيفي لموظفي الإدارة العليا جاء بدرجة مرتفعة وبنسبة تقييم بلغت (70.8%) ، كما وتبين وجود علاقة طردية ذات دلالة احصائية بين تطبيق التفكير الإستراتيجي ومستوى الأداء الوظيفي في وزارة التربية والتعليم الفلسطينية. وفي ضوء النتائج التي توصلت إليها الدراسة، قُدمت مجموعة من التوصيات أبرزها على وزارة التربية والتعليم الفلسطينية تبني ثقافة التفكير الاستراتيجي ونشرها بين العاملين في كافة المستويات ومنحنهم صلاحيات تمكنهم من ممارسة التفكير الاستراتيجي. كما وعليها تعزيز العاملين ذوي الأداء الوظيفي المرتفع وتحفيزهم باستمرار ورصد احتياجاتهم وتدريبهم والذي ينعكس بشكل ايجابي في تحقيق أهداف وزارة التربية والتعليم الفلسطينية. الكلمات المفتاحية: التفكير الاستراتيجي ، الاداء الوظيفي ، التعليم في فلسطين
Indo-Pak subcontinent is the world‘s largest cultivator of Brassica. It constitutes 33 % of the world‘s land, cultivated under rapeseed and mustard crops. Weeds ecology of Brassica crops i.e., Brassica juncea (L.) Czern. (Mustard), B. rapa L. and B. napus L. (Rapeseed) were explored for elaborating the weed species profile in districts of Abbottabad, Haripur and Mansehra. Weeds are the part of dynamic ecosystem growing in cultivated crop or ordinary environment. Its flora has not been evaluated quite often from its ecological point of view and phytosociological classification because of its deteriorating impacts on economic crops. Therefore, its flora in Brassica crop of the aforementioned districts was quantitatively analyzed under the impact of various environmental variables. Quadrate quantitative ecological techniques were used for sampling of weeds vegetation. Phytosociological attribute and physiognomic characteristics of each weed species were recorded at each quadrate.Physicochemical properties of soil were measured at each station and compared with quantitative and synthetic characteristics of weed communities. A total of 50 weed species (44 dicot & 6 monocot) were recorded from Brassica crops belonging to 20 different plant families. The topmost abundant weed of the Brassica crop was Euphorbia helioscopia, Galium aparine and Coronopsus didymus while, Lapsana communis, Scandix pecten veneris and Parthenim hysterophorus were the rare weed species with minimum importance values. Euphorbiaceae was the topmost dominant family followed by Poaceae and Brassicaceae in the studied area. All the collected weeds belong from therophytes life form category. Species belonging to Constancy Classes IV-V were Euphorbia helioscopia, Coronopus didymus and Fumaria officinalis appearing close to 50 % of the stands in Mustard and Rapeseed fields. While Anagallis arvensis, Avena sativa and Galium aparine present in less than 50% of the stands (Class: III - IV). Species revealed to Class I, were present either in Mustard or Rapeseed fields. It appears that the synthetic characters followthe pattern of Quantitative Characers upto certain extent. Result of soil characteristics revealed that the soil of Abbottabad and Mansehra districts were mostly silty clay loam different from Haripur and Havelian regions along with silt loam soil condition. Furthermore, soil of Abbottabad and Haripur has maximum amount of N-NO3, pH as compared to Mansehra. Potassium (K) and Phosphorus (P) concentration were higher in soil of Mansehra and Haripur rather than Abbottabad Havelian regions.Regarding, Zinc (Zn) and Manganese (Mn) concentration which is higher in Haripur and Mansehra respectively. Electrical conductivity (EC) was higher in all three regions except Haripur.While, increased amount of nitrate concentration in soil is due to the abundantly presence of nitrogen fixation weeds in the fields. The aforementioned finding i.e., weeds of Brassica crops, their general profile and distribution in different farming systems of the area will provide a baseline for devising weed management strategies in the studied area. It will also open a new venue of research for Brassica crops improvement through the scientific ways of weed eradication