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Home > Biochemical and Immunochemical Characteristics of Wheat Gluten

Biochemical and Immunochemical Characteristics of Wheat Gluten

Thesis Info

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Author

Nadeem, Muhammad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1336

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725584099

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The current research work was undertaken to characterize different Pakistani wheat varieties for physicochemical, rheological, technological and immunochemical characteristics. The modification of wheat gluten through methionine binding to develop bread for celiac disease patients was also the main aim of this study. The results revealed that wheat varieties differ significantly for the characteristics studied. Significant effect of crop year was observed on grain width, moisture content, falling number value and dough rheological parameters. The antibody response against glutenin and HMW-GS was positively correlated to the total score of breads, chapattis and naans. The electrophoretic patterns of molecular weight of glutenin subunits of different wheat varieties showed the presence of glutenin subunits in the range of 28.23 to 110.89 kDa and 28.29 to 113.51 kDa, while total gliadin electrophoregrams ranged from 31.03 to 89.61 kDa and 32.91 to 92.22 kDa among different wheat varieties during the crop years 2010-11 and 2011-12, respectively. The wheat variety AARI-11 outperformed other varieties in most of the physico-chemical, rheological and technological characteristics. Therefore, it was selected for gluten modification. The immunoreactivity to the modified gluten proteins was decreased as the reaction proceeds and minimum immunoreactivity (26±0.37%) was observed near to 60 minutes of reaction time with 5% methionine added to the system. The results showed that the serum of each patient had positive IgA index to gliadins from unmodified gluten, but just sera of two patients had positive IgA index to gliadins from modified gluten and when these proteins were digested the sera of no patient’s serum had positive IgA reactivity. The gluten content in breads prepared with modified gluten was observed to be 79 ppm, which is within the limits set by Codex Alimentarius Commission. The study concludes that the methionine binding to gluten induced a remarkable reduction of celiac IgA immunoreactivity to gliadin. ELISA is a reliable method and can be used as single tool for the wheat quality assessment therefore it should be developed for all wheat varieties. Prospectus of modified gluten for different wheat based foodstuffs like the assayed bread in this study needs to be investigated. A research project should also be conducted to investigate the antibody response of modified gluten.
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آئو وطن آباد کریں

آئو وطن آباد کر یں
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اسا تذہ کرام اور میرے ہم مکتب ساتھیو!
آج مجھے جس موضوع پر اظہار خیال کرنا ہے وہ ہے:’’آؤوطن آباد کر یں‘‘
صدرِذی وقار!
وطن کی محبت ایمان سے ہے، وطن سے والہانہ عقیدت ایمان کا حصہ ہے، وطن ہے تو ہم ہیں ، وطن ہے تو ہمارا وجود قائم ہے، وطن کی خوشبو سے ہمارے دماغ معطر رہتے ہیں،وطن کے صحرودر یا ہمارا سرمایہ ہیں، وطن کے شجر وحجر ہمارا اثاثہ ہیں۔
محترم صدر!
اگر یہ الفاظ ہم دِل کی اتھاہ گہرائیوں سے کہتے ہیں، تو ہم قابلِ فخر ہیں، ہماری حیات کی ساعتیں قابلِ صد مبارکباد ہیں، ہمارے وطن کے بارے میں تصورات یقینا صائب ہیں، ہماری محبت واقعی وطن کے لیے حقیقی ہے، ہمارا خیال اپنی سرزمین کے لیے واقعی طلسماتی اور کرشماتی ہے۔
صدرِمحترم!
وطن سے محبت اور وطن کی آبادکاری دماغ کے سوچنے کانام ہیں، وطن کی تعمیر صرف زبان کے اظہار کا نام نہیں ،وطن کے گلشن کی تز ئین صرف جسم کی حرکات کا نام نہیں ، وطن سے محبت اور پیار صرف قول و قرار کانام نہیں۔
معزز سامعین!
وطن سے محبت کرنی ہے تو وطن کے افراد سے محبت کرنا ہوگی ، وطن کے در و دیوار سے محبت کرنا ہوگی ، وطن کے نقصان کو اپنا نقصان سمجھنا ہوگا ، وطن کے مفاد کو اپنے مفادات پر ترجیح دینا ہوگی ، وطن کی تعمیر میں لاثانی اور مثالی کردار ادا کرنا ہو گا کیوں کہ وطن ہی ہماری آن ہے، وطن سے ہماری شان ہے وطن ہے تو ہم ہیں وطن نہیں ہے تو ہم بھی نہیں ہیں کیونکہ یہی وطن ہی تو ہماری شناخت ہے۔
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