Wheat genetic improvement for various economic traits is always a challenging objective for the breeders. It is very crucial to meet the wheat quality and quantity demands considering the continuously increase of population, constrains of land, water and global environmental change. Especially, in the most fastrising markets of China and South-Asia where improvement in kernel quality is becoming much more important than ever, mainly because of fast increase of income and food diversity. The conventional wheat quality traits also require to be evolved by the influx of new processing technologies with much more concern on health aspects. The incorporation of different disciplines like proteomics, functional genomics, genetic transformation, bioinformatics, breeding and utilization of novel genetic resources, is swiftly endorsing our understanding for biochemical and genetic basis of quality encoding wheat attributes. So present project was designed to investigate the existing gene pool in current cultivars of Pakistan and land races and also explore the genetic diversity in Ae. taushcii derived synthetics. The results of phenological characterization showed that land races and synthetics had great potential for yield components as compared to Pak cultivars. In case of land races, LLR-29 and LLR-30 were good candidate genotypes for yield improvement while regarding synthetics SH-1002, SH-23, SH-378, SH-12, SH-829 and SH-905 showed good results for yield characters. For the quality parameters LLR-29, LLR-32, SH-23, SH-423, SH-1002, SH-378, SH-400 and SH-956 performed good during the experiment as compared to existing cultivars of Pakistan. It was also observed that LLR-29, SH-1002, SH-378 and SH-23 had great potential for yield and quality improvements as these genotypes performed their best for both characters. HMW-GS composition and individual allele frequencies showed that in total, 15 x-type and 9 y-type subunits were observed, of which Glu-A1 had 3 x-type ,Glu-B1 had 6 x-type and 5-type subunits and Glu-D1 had 6x-type and 4 y-type subunits. The genetic variation assessed through Nei’s index was highest for Glu-Dt1 locus (0.83) followed by Glu-B1 (0.77) while lowest diversity was observed at Glu-A1 locus which was 0.64. Out of33 different combinations,highest combinations observed was null, 6+8, 2.1+10.5 followed by null, 6+8, 1.5+10 and2*, 6=8, 2+12 observed in6 (11.76 %), 5 (9.80 %) and 5 (9.80 %) in synthetic wheats. Maximum HMW-GS diversity was found in synthetic hexaploid wheats. For LMW-GS, six alleles were found at Glu-A3 locus and nine alleles were found at Glu-B3 locus. Frequently observed allele was Glu-A3c found in 41 (50.61 %) genotypes followed by Glu-A3d and Glu-A3b found in 19 (23.45 %) and 12 (14.81 %) genotypes, respectively while Glu-A3e was only found in 2 (2.46 %) genotypes and was least frequent. At Glu-B3 locus, maximum frequency was observed at Glu-B3h locus that was in 15 (18.51 %) genotypes which was followed by Glu-B3i found in 14 (17.28 %) accessions. While, minimum frequent allele was Glu-B3d, that was observed in only three genotypes (3.70 %). In genetic diversity study by SNPs genotyping, the 90 K SNPs chip had given 35,906 biallelic SNPs, out of which 26,905 SNPs were polymorphic. An average call rate of 97 percent was obtained across all SNPs. Two genotypes SH-551 and SH-843 showed maximum heterozygosity for AB alleles with the percentage of 21 percent and 18 percent, respectively, while their call rate was 91 percent and 95 percent, respectively. A call rate of 98-100 percent with 2-0 percent heterozygosity was observed in the rest of genotypes.
مولانا محمد مبارک علی مولانا محمد مبارک علی صاحب جن کی عمرچھیاسی برس کے قریب ہوگی حضرت شیخ الہند کے شاگردِ خاص اورآپ سے بیعت بھی تھے اورحضرت کی اسارتِ مالٹا کے بعد حضرت الاستاذ مولانا مفتی عزیز الرحمن صاحب ؒ سے تجدیدِ بیعت کرلی تھی اور حضرت مفتی صاحب کے خدام خاص میں شامل ہوکر حضرت موصوف ہی سے طریقہ نقشبندی میں اجازت بیعت و ارشاد حاصل کی۔ایک عرصہ تک ٹونک میں صدر مفتی رہے اورریشمی خطوط کی تحریک سے بھی تعلق خاص رہا۔ غالباً ۱۹۱۹ء میں مدراس (پرناہٹ) تشریف لے گئے تھے اورپھر بریلی کے قدیم مدرسہ مصباح العلوم میں صدارت درس کی خدمت انجام دی۔اب کم وبیش اڑتیس سال سے دارالعلوم دیوبند میں نائب مہتم کے عہدہ پرفائز تھے۔اہتمام و انتظام میں دسترس کے علاوہ کبھی کبھی حدیث کی کسی کتاب کادرس بھی دیتے تھے۔ تقویٰ وطہارت اوراخلاق وشمائل کے لحاظ سے سلف صالحین کانمونہ اورمثال تھے۔ صدحیف!دنیا اب ایسے حضرات سے خالی ہوتی جارہی ہے۔تغمد ھمااﷲ بمغفرتہ ورحمتہ ۔ [ستمبر۱۹۶۸ء]
This study worked on studying the language in modern Jordanian poetry, through application to the poetry of the Jordanian poet Ibrahim Al-Ajlouni through his poetic works, subjected to intertextuality in both its religious and literary parts, as well as addressing repetition in its three types, the sentence, the word, and the letter, then the study ended with the conclusion, which included the most important results that the study reached It has through studying the language of the poet.
Germin-like proteins are glycosylated proteins that are highly diverse in plant kingdom. Most of the germin-like proteins show superoxide dismutase activity. Superoxide dismutase converts superoxide anion into molecular oxygen and hydrogen peroxide. Identification of catalytic residues is an important step for understanding the mechanism of enzyme catalyzed reactions. Previously OsRGLP1 gene and cDNA were isolated from the roots of Oryza sativa and, under CaMV35S promoter, cDNA has been heterologously expressed in tobacco. In transgenic tobacco plants, OsRGLP1 seems to show SOD activity. Present study was aimed to determine the active site of OsRGLP1. Three dimensional structure and residues involved in metal ion and substrate binding were predicted using bioinformatics tools. Three histidines and one glutamate were predicted to play an important role in metal ion binding and Asparagine to Alanine mutation was predicted to be responsible for different substrate specificity of germin and germin-like proteins. These residues were then mutated with other selected residues by site directed mutagenesis to confirm these predictions. In present study out of many designed mutants, four loss of function mutants were further studied by performing transient transformation of tobacco plants. These transgenic plants were used as source of native and mutant proteins for SOD activity assay to find the effect of mutations on SOD activity. Results of SOD assay showed approximately complete loss of activity in all mutant proteins validating the importance of these residues in SOD activity of OsRGLP1 protein. As these residues are involved in metal ion binding so present study also provide some insight into mechanism of action of OsRGLP1 indicating OsRGLP1 indicating the role of metal ion in its activity.