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Home > Biochemical and Radio Labeling Studies of Venom Naja Naja Karachiensis With its Neutralization by Medicinal Plants of Pakistan

Biochemical and Radio Labeling Studies of Venom Naja Naja Karachiensis With its Neutralization by Medicinal Plants of Pakistan

Thesis Info

Access Option

External Link

Author

Hassan Bin Asad, Muhammad Hassham

Program

PhD

Institute

COMSATS University Islamabad

City

Abbottabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7518/1/Muhammad_Hassham_Hassan_Bin_Asad_Pharmacy_2016_HSR_CIIT_20.09.2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725587818

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Biochemical and Radio Labeling Studies of Venom Naja naja karachiensis with its Neutralization by Medicinal Plants of Pakistan Background Snake bite envenomation is one of the vivid examples of neglected occupational hazards that accounts for tens of thousands of deaths all over the world. One of such instance is Naja naja karachisis bite, a nightmare for the inhibitants of Southern Punjab (Paksitan), often endup with countless deaths and sequela. To address this problem present study was designed to highlight scientific grounds for Naja naja karachisis envenomation and to rationalize folklore claimed Pakistani medicinal plants as a first aid treatment before proper hospitalization. Methods Proteomic characterization of Naja naja karchiensis venom was carried out with electrophoresis (SDS-PAGE) and HPLC (SEC & RP-HPLC) coupled LC-MS/MS whereas inorganic constituents were quantified with ICP-OES technique. Bio distribution and kinetic profile of venom was monitored with short lived radiotracer (99mTc) via direct radio isotopic binding technique. Lethal biological effects of crude venom were examined in terms of its LD50, hemolytic and anticoagulant behavior while toxic biochemical parameters (in vivo), towards liver (AST & ALT), heart (CK-MB & LDH) and kidneys (urea & creatinine) damage were investigated by following the recommendations of DGKC and IFCC methods. Venom was analyzed for different enzymatic activities (PLA2, ALPase, 5ʹ-ND, hyaluronidase and protease) by adopting conventional biochemical assays (in vitro). Twenty eight medicinal plants of Pakistan were extracted with methanol by simple maceration process and thereafter used to reverse deleterious actions of cobra venom. RP-HPLC coupled bioassay guided fractionation technique was used to characterize bioactive constituent/metabolite(s), responsible for anti-PLA2 activity in Bauhinia variegata L extract.
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نانی، رضاء الرحمن

آغوش رحمت میں
[ادارہ برہان کے کارکن رضا الرحمن کی نانی کاانتقال]
انتہائی افسوس ہے کہ رضاالرحمن صاحب کارکن مکتبہ برہان کی نانی صاحبہ ۵/ مارچ۱۹۸۴ء صبح دس بجے اس دار فانی سے رخصت کرگئیں۔مرحومہ نہایت متدین، صوم وصلوٰۃ کی پابند اوربہترین معلّہ تھیں۔ خواتین میں شرعی امور کی ترویج کے سلسلے میں مرحومہ معروف تھیں۔ان کی رحلت سے خواتین کے لیے اسلامی مسائل کے لیے ایک خلا پیداہوگیاہے۔
اﷲ تعالیٰ مرحومہ کی مغفرت فرماکر ان کوجنت الفردوس میں اعلیٰ مقام عطافرمائے اورہم پسماندگان کوصبر جمیل کی توفیق بخشے۔(آمین) قارئین سے مرحومہ کے لیے مغفرت کی دعا کے ساتھ نماز جنازہ غائبانہ کی درخواست ہے۔
[مارچ۱۹۸۴ء]

الأبعاد النفسية والتربوية في نظر سيدنا عمر بن الخطاب تجاه القدس

When looking at the history of every human civilization, one may find some people or places that go down in history, and their biography does not disappear until God inherits the land and those on it. One of these places is the city of Jerusalem, a city where no two differ on its importance. A city that is home for sanctuaries, history, and monuments and every inch speaks of its greatness. Especially in the history of Islam, which linked it wonderfully to the Qibla of Muslims (the Sacred Mosque) after it was the first Qibla in the history of Islam. This high standing link was perpetuated by the Holy Qur’an in Surat Al-Isra and in many other places. This research has shown its importance to Muslims, the reason Muslims love it and their dedication to defending every single grain of its sand, and how did the esteemed Companions view it? This research spoke about some of the aspects that are related to the personality of our master Omar bin Al-Khattab, may God be pleased with him, (which are the psychological and educational aspects) while looking at the importance of Jerusalem to him, may God be pleased with him, which existed since pre-Islamic time and how it extended to the time of Islam and has emerged during his caliphate, may God be pleased with him.

Role of Migrants in Making of Modern Faisalabad 1947-1960

Lyallpur is 3rd largest city of Pakistan and 2nd major industrial city contributing major share in revenue of Pakistan. The establishment of Lyallpur is regarded to be associated with canal colonies project of the British to serve their manifold interests including population rationalization, increase in raw material through transformation of barren land into agricultural productive land, extra revenue generation, and reinforcement of strategic supply lines. However, the materialization of canal colony project was accomplished by the migrants of North Punjab who were knack at agricultural savvy second to none. The effective blend of role of government in form of providing conducive to agriculture environment and the migrants’ skill and hard work gave multiplier effect to agricultural growth of Chenab Colony enough to turn it into grainery of Asia. The study has discussed locality of Lyallpur district under paradigm of role of migrants in defining and restructuring social and economic dynamics of the host locality. The locality serves to be interesting site of study under this paradigm as it observed two large scale migrations who took place under different circumstances and for different objective. Hence it provided potent case study to verify significance of migrants in redefining and restructuring social and economic dynamics of Lyallpur. It has further looked into status of Lyallpur in partition of Punjab 1947 and stakes of the Sikh community (migrants from Northern Punjab) in the host locality. It has investigated journey of Lyallpur from ‘Jangli land’ to miracle of Punjab and later on, its transformation to agricultural hub of Punjab earning the status of ‘Manchester of Pakistan’. The study has argued that journey of Lyallpur from barren lands to grainery of Asia was outcome of the planned migration from North Punjab comprising Agriculturally adroit brain drain. It comprised a qualitatively significant and acclaimed portion of Sikh Jats. In wake of Partition of Punjab that was though practiced owing to repetitive demand of Sikh community; they resisted to secure their religious, social, and economic interests in Canal colonies especially Lyallpur. However, they ultimately failed to secure it owing to some logical gaps and practical complexities in their narrative. It resulted into large scale outmigration of non-Muslim community of Lyallpur to East Punjab that was replaced by influx of the migrants from East Punjab. However, the study has argued that though the second migration was abrupt, unplanned, and violent; yet it accompanied with accidental brain drain that comprised industrial and industrious migrants. These migrants by the dint of their hard work and skills not only carried forward agricultural legacy of the district; but they redefined social and economic dynamics of Lyallpur by transforming its economy from primary sector to secondary sector economy. The migrants invested their capital, skills, and time to earn the status of Manchester of Pakistan for Lyallpur.