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Home > Biochemical Profiling and Synergistic Potential of Medicinal Plants As Hepatoprotective

Biochemical Profiling and Synergistic Potential of Medicinal Plants As Hepatoprotective

Thesis Info

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Author

Abid Ali

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/6912/1/Abid_Ali_Biochemistry_UAF_2015.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725594000

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The objective of this study was to find out any alternative natural product to address the hepatic problems. Though many medicinal plants have been reported for hepatoprotective potential but still there is a need to explore further their medicinal effect on scientific basis. Hepatoprotective potential of selected medicinal plants (Silybum marianum, Artemisia absinthium and Rheum emodi) in different combinations against paracetamol induced liver damage was studied. The phytochemical constituents of selected medicinal plants in combinations were tested by measuring total phenolic content (276.26 to 356.57 GAE) and total flavonoid content (13.01 to 25.63 CE). The antioxidant activity of selected medicinal plants in combination was evaluated through DPPH (76.81 to 92.33 % inhibition) and reducing power in term of absorbance (0.964 to 1.652). The antioxidant effect of medicinal plants at cellular level was further explored using H2O2 induced DNA damage on pUC19 plasmid DNA. It was found that the protective effect of plants extracts was concentration dependent. The safety of medicinal plants was evaluated through various toxicological assays including hemolytic, brine shrimp lethality and mutagenicity. The cytotoxicity findings showed that the plants are safe and may be used as medicine. Biochemical profiling of selected medicinal plants was performed through liquid chromatography mass spectrometry (LCMS). The hepatotoxin, paracetamol significantly increased the levels of SGPT, SGOT and ALP whereas the levels of protein and albumin were decreased. The combination containing silymarin extracted from S. marianum, aqueous mixture of both A. absinthium and R. emodi were given to experimental animals with different doses. The hepatoprotective effect was evaluated by studying various biochemical parameters (SGPT, SGOT, ALP, Protein and Albumin). The administration of medicinal plants in various combinations altered the biochemical markers and exhibited significant hepatoprotective activity. The experimental animals were slaughtered at the end of study. The levels of antioxidant enzymes, superoxide dismutase, glutathione peroxidase and catalase were decreased in positive control group (paracetamol treated). The levels of antioxidants enzymes in medicinal plants treated groups remained in normal limit as in the control group. Histopathological examination of liver tissues of control and treated animals showed significant difference indicating that the combinations of selected medicinal plants successfully restored the liver functions. Although majority of the combinations restored the liver functions but the most suitable combination was the least quantity i.e. 100 mg of each plant. It is concluded that the medicinal plants in combination used in this study is an effective and promising formulation against paracetamol induced liver damage.
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اعجاز صدیقی

اعجاز صدیقی مرحوم
( سید شہاب الدین دسنوی)
مولانا سیماب اکبر آبادی کے فرزند اور رسالہ ’’شاعر‘‘ کے مدیر، اعجاز صدیقی پر ۹؍ فروری ۱۹۷۸؁ء کو دل کا دورہ پڑا اور وہ اسی روز اپنے مالک حقیقی سے جاملے۔
ایک عرصہ سے مختلف امراض کی وجہ سے اعجاز صاحب کی صحت خراب ہوچکی تھی، کئی بار اسپتال میں داخل کئے گئے، اچھے اچھے ڈاکٹروں نے بڑی توجہ اور شفقت سے علاج کیا، مگر بقول شاعر:
الٹی ہوگئیں سب تدبیریں کچھ نہ دوا نے کام کیا
دیکھا ، اس بیماری دل نے آخر کام تمام کیا
اعجاز صدیقی فروری ۱۹۵۱؁ء میں اپنے وطن آگرہ سے بمبئی آئے اور یہیں انھوں نے مستقل سکونت اختیار کرلی، ان کا رسالہ ’’شاعر‘‘ (ماہنامہ) جو پہلے آگرے سے نکلتا تھا، اسی سال سے بمبئی سے شائع ہونے لگا، وہ اس کے معیار کو بلند رکھنے میں انتھک، کوشش کرتے تھے، اردو سے پر خلوص محبت اور اپنے قارئین کو صاف ستھرا ادب پیش کرنے کی کوشش، ان کی زندگی کے دو ایسے نمایاں پہلو تھے کہ جن کی وجہ سے اردو کے اچھے اور اہم لکھنے والوں اور شعراء کا انھیں غیر معمولی تعاون حاصل ہوتا رہا، جس کے سہارے وہ ’’شاعر‘‘ کے بڑے ضخیم خصوصی نمبر نکال سکے، ان میں کرشن چند نمبر، ناولٹ نمبر، افسانہ اور ڈرامہ نمبر اور آخری میں ہم عصر اردو ادب نمبر ہماری زبان و ادب میں قابل قدر اضافہ ہیں، حقیقت یہ ہے کہ مسلسل علالت گرتی ہوئی صحت اور محدود مسائل کے ساتھ ایسے ضخیم اور اچھے نمبر شائع کرنا، بڑی جرأت کا کام تھا، بلاشبہ اعجاز صاحب غیر معمولی قوت ارادی کے حامل تھے۔
اعجاز صدیقی، ذاتی طور پر مشرقی تہذیب اور قدروں کے علمبردار اور رکھ رکھاؤ کے آدمی تھے، انھوں نے لوگوں کے ساتھ اپنے تعلقات اور دوستانہ رسم سالہا سال...

COMPARISON OF SCIATIC NERVE GLIDING AND LOWER EXTREMITY DYNAMIC STRETCH ON HAMSTRING FLEXIBILITY IN ATHLETES

Background: Hamstring muscles are targeted among football players. Sciatic nerve gliding improve hamstring flexibility among football players. Objective: To compare the effects of sciatic nerve gliding and lower extremity stretching on hamstring flexibility among football players. Methodology: In this randomized clinical trial 20 male athletes were selected which divided in two groups. One group was given sciatic nerve gliding while other lower extremity stretches. Athletes with age of 18-30 years, male foot ballers who regularly exercise 2 to 3 times a week for 30 minutes for 6 weeks were included. Goniometer was used to collect data by performing straight leg raise and Active knee extension test. Results: The results showed that the mean age and SD was 21.30±1.809. Comparison of treatment within group was checked by Wilcoxon Rank test showed significant results (P was less than 0.05 )and between group comparisons was checked by Mann Whitney test and found sciatic nerve gliding gave more improvement in Athletes performance (  p value was less than 0.05 for straight leg raising and active knee extension test. Conclusion: It was concluded that sciatic nerve gliding gave more effective results than lower stretching in increasing hamstring flexibility among football players.

Effect of Tillage and Farm Manure on Soil Properties, Crop Growth and Carbon Sequestration under Wheat-Rice System

Pakistan is predominantly an arid country but it has one of the best and extensive irrigation systems in the world. Wheat (Triticum aestivum L.) and rice (Oryza sativa L.) are the major grain crops in the country and wheat-rice cropping pattern is followed on substantial area of the country. Soil health directly controls the growth and development of crops and so for wheat and rice. The key of sustainable agricultural production is the optimum use of on-farm resources to feed ever increasing population without harming the available resources. For seed bed preparation the use of different tillage methods is most likely and is in practice for many years. Depending upon soil type and agro- ecological conditions of a particular area, appropriate tillage methods should be selected. Soil quality is affected by tillage and farm manure application, similarly, crop growth and yield is also controlled to various degrees. In order to assess the effect of farm manure, compost and inorganic fertilizer on the growth and yield of wheat and rice crops, pot studies were conducted. The promising level of farm manures from these studies and from previous literature were chosen for field studies. The use of different tillage systems is common practice of the regional farmers. The effects of tillage and farm manure on crop growth and yield, soil physical properties and C-sequestration under wheat-rice cropping rotation were investigated at Faisalabad. Three tillage methods (minimum, conventional and deep tillage) and three farm manure at 0 (control), 15 and 30 Mg ha–1 was used to evaluate the effect on soil physical properties and growth. The wheat and rice crops were sown in pots and field up to maturity. Different agronomic, yield parameters and chemical composition were recorded at the harvest of each crop. Soil samples (0-100 cm) were collected before and after every crop from each treatment plot and were analyzed for NPK concentrations. Soil bulk density and hydraulic conductivity was improved by the use of farm manure. Data were analyzed statistically following standard statistical procedures analysis of variance and multivariate analysis of variance and bi plots were constructed. The root length density (RLD) of wheat in upper 10 cm soil layer was 16% more in case of minimum tillage (MT) with farm manure at 30 Mg ha–1 (FM30). The total RLD of rice the same soil layer was increased by 12% and 17% in case of deep tillage with FM at 15 Mg ha–1. The rice yield was increased by 24% under minimum tillage with FM30 during first year and 21% at 15 Mg ha–1 FM under same tillage. The increase in wheat yield was 11% under MT with farm manure at 15 Mg ha–1 (FM15) during second year. The conventional tillage (CT) with FM30 increased the carbon sequestration among all the tillage systems. The information will be used to further evaluate the amount, direction and change in carbon turnover in all the cropping systems of the country to alleviate the resource poor farmers of the country and in the long run to assess the value of different indicators in relation to soil quality and crop productivity. The treatment combinations CT × FM0 (first year) and CT × FM15 (second year) were more economical incase of wheat. CT × FM15 also proved its worth incase of rice for both years.