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Home > Biochemical Studies on Berberis Lyceum Royal and Analysis of its Extracts for Bioactivity

Biochemical Studies on Berberis Lyceum Royal and Analysis of its Extracts for Bioactivity

Thesis Info

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Author

Asif Ahmed

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2009

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2811/1/302S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725596118

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Medicinal plants are major source of drugs used for the treatment of various health disorders. Berberis lyceum Royal, an indigenous plant of the North-East of Pakistan was selected to explore its medicinal value during this study. This plant has many therapeutic values and is being used against many diseases / infections by local population since centuries. B.lyceum remedies provided against swollen and sore eyes, broken bones, wounds, gonorrhea, curative piles, unhealthy ulcers, acute conjunctive, and in chronic ophthalmia. Therefore, thorough investigation was conducted for proximate analysis, fatty acid contents, metal ion analysis, isolation and purification of alkaloids. Bioactivity of crude extract for antimicrobial, antidiabetic and wound healing have been investigated in this study. Biochemical analysis of root samples of B. lyceum Royal showed the variation among different parameters, which include protein contents (4.4 – 6.24 %), crude fiber (14.96 – 16.40 %) and crude ash (3.79 – 6.99 %) on dry weight basis. No variation regarding crude fats (0.5 %) was found in any samples analyzed. The oil contents were determined by Soxhlet method and results revealed that the principal saturated and unsaturated fatty acid components of B. lyceum Royal root were Palmitic (16:0), Oleic (18:1) and Linoleic (18:2) acids. Palmitic acid (11.73 – 32.04 %), stearic acid (1.09 – 2.66 %), oleic acid (12.01 – 39.67 %), Linoleic acid (42.59 – 47.43 %) and linolenic acid (1.70 – 5.71) were found when oil was analyzed by gas chromatography-mass spectrometry. In all cases polyunsaturated fatty acids (PUFAs) were greater than monounsaturated fatty acids (MUFAs). The micro and macro elements of different samples were analyzed by atomic absorption spectrometry and flame photometer. The results showed that the higher mineral ion contents under investigation were found in Mansehra sample i.e. 599.12 μg /g, whereas Abbotabad had the lowest content, 242.63 μg/g. The total mineral ion contents was in the sequence of Mansehra> Kotlisattian> Bagh> Abbotabad. Calcium (Ca2+) was the highest, ranging from 456 to 187.33 μg/g and copper (Cu2+) was the lowest, ranging from 0.37 to 0.013 μg/g. Two alkaloids, berberine and palmatine were analyzed and quantified by TLC and HPLC and proton and carbon signals were detected in 1H and 13C -NMR spectra. The analysis of the NMR spectra of berberine and Palmatine revealed that the proton H-13 resonating as a singlet (H-13 of 1: δ 8.72; H-13 of 2: δ 8.81) could be used for quantification. The 1H NMR method used in this study was found to be simple, rapid and specific for the analysis of protoberberine alkaloids and required reference compound, apart from the internal standard, and an overall profile of the preparation was obtained directly. Using this method the content of protoberberine alkaloids can be determined in Berberis lyceum and other plant extracts in a shorter time than conventional method of HPLC. Bioactivity of crude extract and Berberine of B. lyceum Royal was evaluated for antimicrobial, antidiabetic and wound healing. For antimicrobial bioassay, root extracts of B. lyceum prepared in three different solvents, methanol, ethanol and aqueous and tested against different bacteria, fungi and yeast strains. Antimicrobial activities were assessed by using Disc diffusion method and Micro dilution assays. It was observed that all root extracts of Berberis lyceum were highly effective against different bacteria and fungi. The methanolic and ethanolic extracts have inhibited growth of microorganisms more effectively as compared to aqueous extract. The results obtained in present study indicates that root of B. lyceum contained some phytochemicals having antimicrobial activity and could be used for pharmaceutical industries for the development of new drugs required for human and animal health. The wound healing activities of the aqueous and methanol extracts of the root of B. lyceum were assessed using incision, excision and dead wound space models of wound repair in rats. After application of both extracts it was observed that the area of epithelialization increased, followed by an increase in wound contraction, skin breaking strength, tissue granulation, dry weight and hydroxyproline content. Histopathological studies of the granulation tissue also indicated that there was an increase in collagen formation in those rats treated with the methanol extract, compared with the control group animals. The methanol extract was more effective than the aqueous extract, but both showed significant results as compared to the control. The antidiabetic activity of the ethanol root extract of Berberis lyceum was compared with pure berberine in normal and alloxan-diabetic rats using similar doses of each. The purpose of the study was to investigate the effects of berberine and a whole extract of B. lyceum on blood glucose and other parameters associated with diabetes, to compare the effects of the crude extract with those of pure berberine and thus validate its use as a therapeutic agent, and finally to identify any contribution of the other components of the extract to these effects. Oral administration of 50 mg/kg of Berberis extract and berberine to normal and experimental diabetic rats produced a significant (p < 0.05) reduction in blood glucose levels from days 3 –7 days of treatment. Significant effects were also observed on the glucose tolerance, glycosylated haemoglobin, serum lipid profiles and body weight of experimental animals. Berberis extract and berberine demonstrated similar effects on all parameters measured, and although the extract was comparable in efficacy to berberine, it did not produce any effects additional to those shown by pure berberine. The results support the use of the extract in traditional medicine, and demonstrate that apart from being a highly cost- effective means of treating with berberine, as compared to root extract which is cheaper, easily available to rural community and also show no or very less adverse effects as compared to pure compound (berberine).
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قاضی محمد جلیل عباسی

قاضی محمد جلیل عباسی
افسوس ہے گزشتہ مہینے میں دو دنوں کے وقفے سے ملک و ملت اور علم و دین کے دو خادم ہم سے جدا ہوئے، جناب قاضی محمد جلیل عباسی نے طویل علالت کے بعد ۷؍ نومبر کو لکھنؤ میں داعی اجل کو لبیک کہا۔ وہ مشہور قومی و ملی کارکن، اردو تحریک کے ممتاز رہنما اور دینی تعلیمی کونسل کے بانی قاضی محمد عدیل عباسی مرحوم کے چھوٹے بھائی تھے۔ دونوں بھائیوں نے اپنے وطن بستی (سدھارت نگر) کی ترقی و خوش حالی کے لئے گوناگوں مفید کام کئے، قاضی جلیل عباسی بھی اپنے بڑے بھائی کی طرح فرقہ ورانہ سیاست سے دور اور کانگریس سے وابستہ رہے، ان کی زندگی قومی خدمت کے لئے وقف تھی۔ ایک زمانے میں ریاستی وزیر اور پھر پارلیمنٹ کے رکن منتخب ہوئے۔ شرافت، ہم دردی، بے لوث خدمت کے ساتھ ان کا تعلق دین و مذہب سے بھی ہمیشہ رہا، اﷲ تعالیٰ قوم و ملت کے اس خادم کی مغفرت فرمائے، آمین۔ (ضیاء الدین اصلاحی، دسمبر ۱۹۹۶ء)

 

''المواھب اللدنیۃ با لمنح المحمدیۃﷺ'' کا تعارفی و تجزیاتی مطالعہ An Analytical study of the

Although Imam Qusṭalānī, in compiling his book "Al-Mawāhib Al-Laduniyat Bil Minḥ Al-Muhammadiyah", has followed the footsteps of Qazi Ayaz’s book Al- Shifā. But many chapters and information are unique to them and he has expanded this book with his additions and made it a treasure trove of information. This book is a beautiful fusion of the traditions of Muhaddithin and Ahl-e-Siyyar. Because he was not only a muhaddith but also a biographer. Were his greatest service in the learning of Hadith is "Irshad Al-Sārī Sharh Saḥiḥ Bukhārī", there his most significant service in Sira is this book. He has used the traditions of both the narrators and the Biographers in compiling it. In this book, the locks of meanings are opened with the keys of Fatḥ Al-Bārī Li Ibn-e-Hajar ‘Asqalānī. In other words, he has benefited a lot from Fatḥ Al-Bārī Sharḥ Bukhari and has gained a lot of confidence. A large number of scholars have used this book, including Shiblī Nu‘mānī, who has made extensive use of it in his Sirat un-Nabiﷺ . Due to its popularity, many of its rates have been written. The most detailed of these is the Zurqānī rate of Qusṭalānī. The great scholar like Imam Zarqani has commented in eleven volumes of this book, which is proof of its authenticity, noteworthy, and worth reading and treasure. Sheikh Nūruddin Ṭrabulsī gave Sharh and great people like Safiuddin Qasashi, Burhanuddin Ibrahim Maimoni, Shamsuddin Muhammad Shobri Misri and Nooruddin Ali Qari embellished this book with their footnotes. On the one hand, it teaches love and respect for the Holy Prophet ﷺ and on the other hand, it mentions the rights of the Holy Prophet ﷺ and the rewards for their payment. The writing style of the book is simple and smooth as well as eloquent and eloquent. The temptation is not so long that the length will be too long for the reader, nor is it so short that access to the concepts and demands will not be possible due to the brevity. The book has been read by the people and it has been adopted by biographers as an authentic and reliable source. Because of its importance and usefulness, an introductory and analytical study of this book will be presented in this article.

Pakistans Cotton and Textiles Exports to the European Union: Competition, Trade Barriers, Future Prospects and Outlook

The study, which primarily aimed at analyzing the European Union’s imports from Pakistan, finds that there exists a strong correlation between the EU’s total textiles and clothing imports and her imports from Pakistan. Same is true as far as the various categories of 26, 65 and 84 in textiles and clothing imports are concerned. Pakistan’s performance seems satisfactory especially in case of 26-category textiles, while the imports of 65-category and 84-category textiles need improvement. It is good for Pakistan that her rates of growth in 65 and 84 categories of textiles imports by the EU are relatively higher than the EU’s own total imports. Pakistan needs to maintain not only its present growth rates but she should also make efforts to get more shares, especially in 65 and 84 category textiles in EU market, as these two category belong to value added textiles. The study identifies 45, 217 and 94 the EU’s preferred 5-digit textile and clothing products, and reveals that eight Pakistani 5-digit 26-category products (with SITC codes 26339, 26310, 26902, 26490, 26721, 26901, 26652 and 26711) have attained positions in the EU’s first 20 preferred products list, while remaining 12 the EU’s preferred products have fallen into Pakistan’s relatively less preferred products list. In case of 217 5-digit 65-category products, only 14 Pakistani products (65843, 65841, 65842, 65221, 65847, 65234, 65133, 65243, 65851, 65232, 65242, 65184, 65892, and 65859) have got positions in the EU’s first 50 preferred products list. In case of the EU’s preferred 5-digit 84-category textiles products, only three Pakistani products (84140, 84629 and 84371) have attained positions in the EU’s first 20 preferred products list. These facts warrant attention of Pakistan’s textiles and clothing manufacturer, exporters and policy makers for improving quality for meeting the EU’s standards. The study finds that the EU has imposed different levels of tariffs based on the primary commodities, semi-processed and processed products, and these rates are enhanced along with the levels of processing of the products, and Pakistan is adversely suffering because of the stated the EU’s policy. In addition, the EU has put various countries in three different schemes of Generalised System of Preferences (GSP) namely, standard GSP arrangements, GSP + and EBA. Pakistan imports to the EU are presently facing general GSP arrangements, and is not benefiting from the other two relatively more beneficial schemes, presently available to a number of Pakistan’s competitors in textiles. Pakistan understands that her imports to the EU are subject to a number of non-tariff barriers (NTBs) including various standard procedures set under WTOs agreements in the disciplines of government procurement, services, investment, trade facilitation, trade defence, standards, intellectual property rights, competition policy, and rules of origin. From Pakistan’s point of view, if the EU goes for having FTAs with some of Pakistani viicompetitors, then Pakistan would face serious adverse effects due especially to the WTO related standard procedures. Based on findings of the study and conclusions drawn, this study forwards certain recommendations. Study finds Pakistan’s performance especially in cases of 26 and 65-category textiles satisfactory, and recommends that the imports of 84-category textiles from Pakistan to the EU should also be given priority, being the value added products. In case of the individual major categories (26, 65 & 84), the following 5-digit products, being the EU’s most preferred products, should be given priority, in particular. (a) The eight Pakistani 5-digit 26-category products (SITC codes: 26339, 26310, 26902, 26490, 26721, 26901, 26652 and 26711) have attained positions in EU’s first 20 preferred products list, while remaining 12 the EU’s preferred products (Table 4.30a) have fallen into Pakistan’s relatively less preferred products list; Pakistan should improve the quality of these products to attain better position in the EU’s 5-digit 26-category market. (b) In case of 217 5-digit 65-category products, only 14 Pakistani products (65843, 65841, 65842, 65221, 65847, 65234, 65133, 65243, 65851, 65232, 65242, 65184, 65892, and 65859) have got positions in the EU’s first 50 preferred products list; the remaining 36 the EU’s first category preferred products (Table 4.31a) should now be having attention of Pakistani stakeholders. (c) In case of the EU’s preferred 5-digit 84-category textiles products, only three Pakistani products (84140, 84629 and 84371) have attained positions in the EU’s first 20 preferred products list; the remaining 17 products (Table 4.32a) should be given attention for a greater value-added import of the EU from Pakistan. (d) Policy makers of the EU and Pakistan should take note of the fact that the EU policy of tariff escalation is discouraging value-addition textile trade from Pakistan. They should also take care of the concern that the EU has put Pakistan at disadvantage, comparing to its competitors, by putting Pakistan in the Generalised System of Preferences (standard GSP arrangements), and some of her serious competitors in more advantageous GSP + and EBA schemes. The EU’s officials and their Pakistani counterparts should also settle the problem of the existence of various non-tariff barriers (NTBs), and especially the affairs relating to the adoption of various standard procedures set under WTOs agreements in the disciplines of government procurement, trade in services, investment, trade facilitation, trade defence, products and processes quality standards, intellectual property rights, competition policy, and rules of origin. The EU should particularly be careful while going to have FTAs with some of Pakistan’s competitors as Pakistan would face serious adverse effects due especially to the non- or partial-adoption of the stated WTO related standard procedures. Pakistani stakeholders, especially the government, should take appropriate steps for an early and extensive adoption of WTO agreements and related standards and standard procedures. (e) Pakistani stakeholders (manufacturers, exporters and policy makers) would have to adopt the required and up-to-date quality standards (relating to the product, process and packing) while keeping costs under control. Government of Pakistan in particular should take arrangements for having inflation in control and making availability of inputs and resources ensured.