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Home > Biomineralization of Black Shale Using Indeginous Microbes

Biomineralization of Black Shale Using Indeginous Microbes

Thesis Info

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Author

Fozia Anjum

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1610

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725635385

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The main concern of the present research work was to find a more feasible and economical method to extract metal ions from low grade discarded ores like black shale by Aspergillus niger and Penicillium notatum. Major elements present on the exposed surface of black shale identified by EDX study were iron, silicon, aluminum, copper and oxygen. Black shale ore mainly contained aluminosilicate minerals such as illite, kaolinite and muscovite and sulphide minerals were pyrite, pyrhotite, Ni-sulphide, sphelrite and chalcopyrite. Oxide minerals like quartz, aluminum oxide, wollastonite and dolomite were also detected by XRD. Raw sample mainly composed of (mgL -1 ) Al 8799.00, Fe 3761.00, Cu 39.07, Co 18.26, Zn 31.88 and Ni 8.25. Different agricultural wastes in the presence of sulphuric acid as substrates were evaluated in organic acid production in shaking flasks with and without ultrasonic treatment. Comparatively, A. niger exhibited a good potential in generating varieties of organic acids effective for metal ions solubilization in case of samples treated ultrasonically. Maximum recovery of metals was achieved in samples treated ultrasonically in both cases of microbes. In case of A. niger, maximum solubilization of aluminum (93.12 %), iron (90.12 %) and zinc (89.41 %) was achieved in the optimized medium of molasses containing 5 % level of ore treated ultrasonically whereas copper (94.03 %) was extracted in sample containing optimized mango peel at 5 % level of ore density. Cobalt recovery (93.32 %) was found in medium having 8 % level of seed cake treated ultrasonically whereas in case of P. notatum, maximum recovery of aluminum (69.23 %) was achieved in optimized seed cake media at 3 % level of ore density. Iron (72.35 %) was recovered in media having 8 % level of mango peel. Copper (68.88 %) and Cobalt (86.42 %) recovery was detected in optimized molasses at 5 and 4% ore density respectively whereas zinc (78.70 %) was extracted maximum in medium having optimized mango peel at 5 % level of ore density. Overall, A. niger showed better potential to extract metals from ore compared to P. notatum. Ultrasonic wave treatment not only increases the growth of microbes and organic acid production but metal ions dissolution from the ore matrices indicated that this low grade discarded ore may be the potential source for metals in future panorama.
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حقیقت کا اقرار

حقیقت کا اقرار
استفہام کا ایک بلیغ موقع استعمال وہ بھی ہے جب مخاطب سے کسی ایسی بات کا اقرار کرانا ہو جس کی نوعیت ہو تو ایک بدیہی حقیقت کی لیکن مخاطب اس کو تسلیم کرنے کے باوجود عملاً اس سے منحرف ہو۔ جیسے آیت ذیل :
" هَلْ أَتَى عَلَى الْإِنْسَانِ حِينٌ مِنَ الدَّهْرِ لَمْ يَكُنْ شَيْئًا مَذْكُورًا" “[ ]
"انسان پر کبھی ایسا وقت آیاہے کہ نہیں جب وہ کوئی قابل چیز نہیں تھا" کی تفسیر میں مولانا امین اصلاحی کانقطہ نظر ہے یہ محض ایک سادہ خبریہ جملہ نہیں ہے بلکہ اس کے اندر بہت سے معانی مضمر ہیں۔ مثلاً:
۱۔ ایک عظیم حق کی یاددہانی ہے جو اس پر عائد ہوتا ہے اور جس سے اس کے لیے انکار کی گنجائش نہیں ہے لیکن اس کا رویہ اس کے منافی ہے۔
۲۔اس میں ملامت ‘ غصہ ‘ رنج اور اظہار حسرت کے بھی گوناگوں پہلو ہیں۔
۳۔اس میں نہایت مبنی برحقیقت گلہ و شکوہ بھی ہے اور نہایت موثر اپیل بھی۔
۴۔ استفہامیہ اسلوب نے اس حسن فکر کو بیدار کرنا چاہا ہے کہ انسان کی نظروں سے خدا اوجھل ہے تو اس کا اپنا وجود تو اوجھل نہیں ہے ‘ وہ خود اپنے اندر خدا کی قدرت و حکمت اور اس کے عدل و رحمت کی نشانیاں دیکھ سکتا ہے۔[ ]
" قُلْ لِمَنْ مَا فِي السَّمَاوَاتِ وَالْأَرْضِ قُلْ لِلَّهِ" “[ ]
"ان سے پوچھوکہ آسمانوں اور زمین میں جو کچھ ہے وہ کس کی ملکیت ہے(پھر اگر وہ جواب نہ دیں توخود ہی )کہہ دو کہ اللہ ہی کی ملکیت ہے"۔
مولانا امین احسن اصلاحی کی تحقیق کےمطابق قرآن مجید میں جہاں جہاں سوال کر کے مخاطب کے جواب کا انتظار کیے بغیر خود اس کا جواب دیا ہے۔ غور کرنے سے معلوم ہوتا ہے کہ...

MANAGEMENT OF AUTISM SPECTRUM DISORDER (ASD) and COLLABORATIVE TEAMS

Autism Spectrum disorder (ASD) is a condition related to the development of the brain that can lead to persistent challenges with socialization, restricted interests, and repeated behaviour. Diagnosis for ASD is used as a basis of DSM-5 (Diagnostic and Statistical Manual of Mental Disorder) produced by American Psychiatric Association. ASD is observed in children during early years of life and significantly affects an individual’s wellbeing. Autism has remained a mystery for many specialists and researchers  for years. Although the cause of autism is still unknown, a number of strategies have been developed that have helped so many people around the world to live independently. So far there are no authentic facts for ASD prevalence in Pakistan however the statistics is roughly calculated from South Asia that ASD might be as common in Pakistan as somewhere else around the world. In a country like Pakistan where Autism is still a new phenomenon especially in rural areas, we have very restricted qualified human resources to provide a reliable rehabilitation service for a child with ASD and their family. Apart from that due to inadequate awareness exposure, knowledge, and incompetency in diagnosis, these children go undiagnosed and are mishandled.

Estimation of Carbon Stocks in Subtropical Managed and Unmanaged Forests of Pakistan

Increasing atmospheric carbon dioxide (CO 2 ) concentrations over the last 150 years and the increasingly dramatic effects of climate change on ecosystems and humankind have reinvigorated the need to understand the terrestrial global carbon cycle. Forest ecosystems are of particular importance because they contain up to 82% of the terrestrial plant biomass, which is inter-linked with atmospheric CO 2 levels through the carbon cycle. This importance has been recognized within global agreements like the Kyoto Protocol, which is designed to address climate change. In order to meet the requirements of this and similar agreements, we need reliable estimates of carbon stocks and fluxes. These have so far been lacking for many of the forest ecosystems of Pakistan. I quantified the mean forest carbon stocks (t ha -1 ) for two major forest types in Pakistan and investigated the uncertainty of these estimates at three levels of sampling intensity. Overall, 261 plots of 1 ha each were established in selected four sites, two in sub-tropical pine forests (Ghoragali, 1729 ha and Lehterar, 1254 ha) and two sub- tropical, broad-leaved, evergreen forests (Sohawa, 4048 ha and Kherimurat, 3360 ha) between 2005-08, representing a sampling intensity of 2.5 percent of the total forest area. In mature (~100 years old) pine forest stand at Ghoragali and Lehterar sites, a mean basal area of 30.38 and 26.11 m 2 ha -1 represented mean volume of 243 and 197xv m 3 ha -1 respectively. The average biomass (t ha -1 ) in both sites was 237 and 186 t ha -1 respectively which is equal to 128 and 100 t C ha -1 including soil C. However, on average basis both the forests have 114.5+ 2.26 t ha -1 of carbon stock which comprises of 92 percent in tree biomass and only 8 % in the top soils. In mixed broad-leaved evergreen forests a mean basal area (m 2 ha -1 ) at Kherimurat and Sohawa was 3.06 and 2.65 with stem volume of 12.86 and 11.40 m 3 ha -1 . The average upper and understorey biomass (t ha -1 ) in both sites was 50.93 and 40.43 t ha -1 respectively which is equal to 31.18 and 24.36 t C ha -1 including soil C stocks. This study provides a protocol and valuable baseline data for monitoring biomass and carbon stocks in Pakistan‟s managed and unmanaged sub-tropical forests. Reducing the sampling intensity from the standard 2.5 percent of total forest area to 1.0, 0.5 and 0.25 percent of total forest area did not increase the coefficient of variation or uncertainty associated with the mean estimated forest C in these forests. However, at a sampling intensity of 0.1 percent there was a marked increase in estimate uncertainty. One of the important recommendations of the study is that 1.0 percent sampling intensity is adequate for future studies in sub tropical pine and broadleaved evergreen forests.