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Biosorptive Potential and Binding Sites of Heavy Metals on the Cell Wall of Selected Filamentous Algae

Thesis Info

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Author

Yaqub, Atif

Program

PhD

Institute

Government College University Lahore

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2008

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1292

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725640822

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Indiscriminate release of heavy metal pollutants into the environment from point and non-point industrial sources has posed a major threat to all kinds of organisms inhabiting aquatic and terrestrial habitats. The application of biosorption i.e., removal of heavy metal ions by the use of biomass, has emerged as a promising technique in the past few years. Utilization of green filamentous algae in this technology still remains largely unexplored. In the present study, the biosorption capacities of biomass of filamentous green algae, Spirogyra cummunis, Cladophora delmatica, and Spirogyra spp. were evaluated for toxic heavy metals, such as Cadmium, Cd (II) and hexavalent Chromium, Cr (VI). The biosorptive binding sites were studied with the help of Scanning Electron Microscopy (SEM) and Fourier Transform Infra-red Spectrometer (FTIR). Results revealed that the rate and extent of uptake were influenced by pH, contact time, and biosorbent concentration. The optimum pH value for uptake of Cd (II) was found to be 5.0 and that of Cr (VI) 4.0 by all the studied biosorbents. The equilibrium sorption data for Cd (II) at pH 5.0 and that of Cd (II) at pH 4.0 were described by various adsorption isotherms, such as Langmuir, Freundlich, and Temkin models. Langmuir isotherm was found to be the best suited for the interpretation of acquired data, showing monolayer adsorption and Freundlich theorem, the worst. Values of Cd (II) sorption capacity, (qmax) for the studied species were found to be 1.44, 11.9 and 14.42 and those of Cr (VI) were 498, 411 and 312, respectively. Kinetic and thermodynamic parameters were also studied. The results showed that pseudo-second order kinetics was suitable for the interpretation of data and thermodynamically biosorption was found to be feasible and spontaneous under the given conditions, in case of all the biosorption investigations undertaken in the present study. SEM and FTIR revealed biosorptive binding sites and possible electronegative functional groups, such as carboxyl, hydroxyl, carbonyl, etc., on the surface of biosorbents which could favor the binding of cations, such as Cd (II) and Cr (VI) ions.
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مولانا ابوسلمہ شفیع احمد

آہ! مولانا ابوسلمہؒ
(پروفیسر مسعود حسن)
۲۲؍ دسمبر کی دوپہر کو کلکتہ کے مسلمان ایک بڑے المناک سانحہ سے دوچار ہوئے، یہ سانحہ حضرت مولانا ابوسلمہ شفیع احمد صاحبؒ کی رحلت ہے، جو ۵-۴ ماہ کی طویل اور صبر آزما علالت کے بعد ۷۳ سال کی عمر میں اپنے مالک حقیقی سے جاملے۔ اناﷲ وانا الیہ راجعون، دوسرے دن صبح ۳۰:۱۲ بجے ان کے ہزاروں عقیدت مندوں اور جان نثاروں نے ان کے جسدخاکی کو پارک سرکس سے متصل ایک قبرستان میں جس کا نام ہم ’گورغریباں‘ ہے، یہ کہہ کر سپردخاک کیا کہ:
اے تیرہ خاک خاطرِ مہماں نگاہ دار
کیں نورچشم ماست کہ دربر گرفتہ ای
نور اﷲ مرقدہٗ و برد اﷲ مضجعہ
مولانا مرحوم کے نام اور مقام سے مجھے بہت پہلے سے واقفیت تھی، البتہ باقاعدہ نیاز اور تقریب ۱۹۵۹؁ء میں حاصل ہوا، جب مجھے مدرسۂ عالیہ کلکتہ کی پرنسپلی تفویض ہوئی اور میں مدرسہ پہنچا، مولانا مدرسہ کے ممتاز ترین اساتذہ میں تھے، تفسیر اور حدیث ان کے خاص موضوع تھے اور ان دونوں فنون کی بیشتر متداول کتابوں پر ان کی گہری نظر تھی، وہ مدرسہ کی اونچی جماعتوں میں درس دیتے تھے اور بڑی عزت و احترام کی نظر سے دیکھے جاتے تھے۔ میں مدرسہ میں کم و بیش ۵ سال رہا۔ اس پوری مدت میں میرے ان کے تعلقات مخلصانہ رہے اور جب میں وہاں سے رخصت ہوا تو اس کے بعد بھی ان کا اخلاص برابر جاری رہا۔ وہ اکثر میرے غریب خانہ پر تشریف لاتے، گھنٹوں بیٹھتے تھے۔ راقم الحروف بھی ان کی خدمت میں حاضر ہوتا تھا، ان ملاقاتوں میں اکثر علمی گفتگو ہوا کرتی تھی، کبھی کبھی حالات حاضرہ پر بھی تبصرہ ہوا کرتے تھے، مولانا ان مجلسوں میں علم و حکمت کے موتی بکھیر دیتے تھے۔
مولانا کو لکھنے پڑھنے اور تصنیف کا شوق...

EFFECTS OF MILD, MODERATE AND INTENSE PHYSICAL ACTIVITY ON KNEE JOINT PROPRIOCEPTION IN HEALTHY YOUNG ADULTS

Aim: To evaluate the immediate effects of various activity levels on knee joint position sense. Methodology: Sixty males aged between 19 and 24 years, without any complains of discomfort or pain in the knee joint were selected from Bakhtawar Amin Hospital, Multan using convenience sampling, between April 2nd, 2019 to July 28th, 2019. Group A participants walked on the treadmill at a speed of 4 km/h for 5 min (n = 20). Group B participants ran on a treadmill at 6.4 km per hour for five minutes (n = 20). Group C participants sprinted on a treadmill at 8.5 km per hour for five minutes (n = 20). Results: Mean age of the subjects was 21.18±1.77. In the moderate physical activity group, the difference in the means in passive reproduction of the knee flexion angle of 20o observed was 1.40±2.39, (p-value 0.013), and similarly in intense physical activity was 1.75±3.58 (p-value 0.020). For the knee flexion angle of 40o, the difference in the means for moderate was 1.35±1.84 (p=0.002), and for the intense activity was 2.35±2.27 (p=0.012). However, in the group with mild physical activity, the difference in the means was statistically insignificant. Conclusion: The study indicated that physical activities of moderate level and intense level decrease the joint position sense appreciation at the knee joint in less active healthy individuals.

Bioactive Potential of Fungus Induced Silybum Marianum Seedlings and Isolation of Antimicrobial Genes

Living organisms are equipped with sophisticated mechanisms to minimize the hazards and threats offered by the nature and maximize their survival potential against strain imposed by environmental forces. Growing concern about the frequent use of analogous antibiotics leading to resistant pathogens and their harmful impacts on crop yield worldwide has fuelled an explosion in the field of plant defense response by coupling biotechnology to the medicinal plants. Increasing knowledge of the mechanisms underlying plant defense response has also encouraged the idea that sensitizing a plant towards microbial elicitors increases plant resistance to the potential pathogens. Considering the same thought of knowledge, this study was entailed to examine the bioactive potential of Silybum marianum under control and fungal stress. Peptide based multidimensional studied of the plant with reference to antimicrobial, antioxidant, antitumor, cytotoxic and mutagenic potential were undertaken. A time course study of the extracts prepared under fungal stress strongly revealed the increased accumulation of proteins/peptides upto 8 hours post inoculation with a drop in the expression level at subsequent hours. Encouraging results regarding antibacterial potential towards gram negative species and highly significant antifungal potential were also observed. In addition to antimicrobial potential, plant also showed beneficial traits as antitumor and nonmutagenic against Agrobacterium tumefaciens and human cell lines respectively. As far as antioxidant and cytotoxic potential is concerned, all the extracts had excellent antioxidant capacity whereas among all, 8 h control and its treated time points showed slight cytotoxicity. Rewarding bioactive potential of Silybum marianum eventually led to the identification of peptides involved in cellular metabolism, plant growth and development, signal transduction and exclusively to the peptides involved in defense response. Antimicrobial gene isolation with subsequent cloning and heterologous expression has proved substantial pharmaceutical importance of the plant which may open novel avenues for biotechnological applications in agriculture.