Helicoverpa armigera (Hubner) is a notorious pest of various fields and horticultural crops worldwide. Insecticides application ultimately responsible for resistance development in H. armigera, unsafe for the environment and natural enemies of insect pests. To withstand the pesticides resistance, biological control successfully helps in reducing pest population. Bracon hebetor is a cosmopolitan species that have the ability to parasitize a wide range of lepidopteran and few coleopteran hosts. As H. armigera fed on wide host range so, quality and quantity of food nutrients provided at the time of Bracon hebetor larval development is a crucial step. The need of the current research project is to focus on which best suited semi-synthetic artificial diet of H. armigera; B. hebetor developmental parameters are good and further, how it can effectively be used under the scenario of ecological conditions after getting reared on efficient host for the control of H. armigera. So we can utilize B. hebetor as an efficient biological control agent for H. armigera management. Study objectives were to screen out the best suited artificial diet of H. armigera for the rearing of B. hebetor. Investigation of B. hebetor life history traits on most efficient hosts from the screened host species for the effective rearing of B. hebetor. Determination of the effect of temperature, relative humidity regimes and host larval densities on the life history traits of B. hebetor parasitizing H. armigera. B. hebetor parasitization, reproduction, development, fitness related traits and life table parameters were observed on different pyralids, noctuids, gelechiids and curculionids under laboratory conditions (25±2°C and 70±5%) in order to find out the most suitable host for the mass rearing and inundative release purposes to control H. armigera. Results indicated that among four semi-synthetic diets (gram, rice, wheat and corn) of H. armigera, gram flour mediated diet was responsible for good biological and developmental parameters of B. hebetor under laboratory conditions. It can be successfully used for the rearing of H. armigera as a host for good parasitism and development of B. hebetor. Though, H. armigera itself was not an excellent host for the continuous rearing of parasitoid therefore, seven different hosts (six from Lepidoptera and one Coleoptera) were utilized to screen out the suitable one. Results showed that the pyralids hosts (E. kuehniella, C. cephalonica and G. mellonella) gave the best results for daily fecundity, parasitoid survival to adulthood, development time and female biased sex ratio followed by H. armigera and the worst reproduction occurred on H. postica. Among the most efficient screened pyralid hosts, B. hebetor biological parameters and fitness related traits were excellent on E. kuehniella as compared to C. cephalonica. So production of good fitness traits (wing area, tibia size and dry mass), excellent biological parameters (good immature numbers and adults emergence), short egg-adult development time and changes in THCs provide a good means for the immature development of B. hebetor within the parasitized E. kuehniella for several days. Temperature relative humidity regimes and H. armigera larval densities significantly affect the biological and developmental parameters of B. hebetor. Excellent life history parameters of B. hebetor parasitizing gram diet fed H. armigera were observed at 25°C and 60% R.H. combination. Among various larval densities of H. armigera good biological parameters of B. hebetor like production of immature numbers, short egg-adult development time, survivorship and female progeny production was significantly good on five larval density of H. armigera offered for parasitization. Therefore, it is recommended that gram mediated diet is best suited for the rearing of H. armigera in order to find out the best suited temperature, humidity combination and host larval density in the field release planning and maintenance of B. hebetor population for long term field crops management to control H. armigera. On the other hand B. hebetor quality progeny production on E. kuehniella make this insect of great potential to serve as valuable material for laboratory rearing and inundative field release to control H. armigera larval populations.
بعد ازاں اپیل کنندہ کے ذریعے عدالت میں ایک تحریری درخواست بھی پیش کی گئی تھی جس میں اس معاملے کے کچھ پہلوئوں پر ذاتی طور پر عدالت سے خطاب کرنے کے موقع کی درخواست کی گئی ۔ اس درخواست کی اجازت دی گئی اور اسی مناسبت سے اپیل کنندہ 1978 ء میں 18 سے 21 دسمبر تک ذاتی طور پر چار دن عدالت میں پیش ہوا ۔ اپیل کنندہ ذوالفقار علی بھٹو نے تقریباً بارہ گھنٹوں پر پھیلے ہوئے وسیع خطاب میں اپنے اوپر لگائے گئے الزامات سے انکار کیا جو کہ بے مقصد، مستغیث اعلیٰ احمد رضا قصوری نے قتل کے گواہ ہونے سے متعلق لگائے تھے۔ اس وقت کے فیڈرل سکیورٹی فورس کے ڈائریکٹر جنرل اور گواہ مسعود محمود جنھوں نے یہ بھی دعویٰ کیا کہ شواہد میں سازش کے جرم کے کسی ضروری اجزا کی موجودگی کا انکشاف نہیں کیا گیا۔ یعنی متفقہ طور پر شریک سازشیوں خصوصاً مسعود محمود کی طرف سے جس نے اپیل کنندہ کی جانب سے سختی سے استدعا کی تھی اپیل کنندہ نے اس پر تبصرہ کیا کہ گواہوں ،احمد رضا قصوری اور مسعود محمود کے ثبوت میں موروثی تضادات موجود ہیں اور عرض کیا کہ وہ ملک میں مارشل لاء کی مجبوری کے تحت کام کر رہے ہیں۔ انھوں نے کہا کہ اگر استغاثہ یہ چاہتا تھا کہ عدالت ملک میں بطور صدر اور وزیر اعظم اپنے عہدے کے دوران پاکستان میں موجود مبینہ معاشرتی حالت کا عدالتی نوٹس لے تو پھر اس طرح کا نوٹس بھی اس حقیقت پر لیا جانا چاہیے جو مارشل لاء کے تسلسل کے دوران مقدمے کی سماعت میں ثبوت اہم گواہ دے رہے تھے۔ انھوں نے سختی سے استدلال کیا کہ ان کے خلاف سارا مقدمہ جھوٹا اور من...
This study aims to analyze and examine the influence of employee commitment and cultural organizational factors towards employees of the Job Performance, Regional Secretariat (SetDa) Riau Province both simultaneously (overall test) and partial (individual test). The research method used is verification, while the population in this study is the Regional Secretariat (SetDa) Riau Province based on increasing totaling 402 people. By using the method of proportional stratified random sampling in the sample amount to obtain 40 respondents. While data collection technology is used by the field study and library that includes observation, interviews and question-naires, and to determine the relationship and the influence of an independent variable to the model variables to use Multiple Linear Regression Analysis. The results showed that the calculated results obtained statistically Multiple Correlation Coefficient (R) was 0.8950 with p <0.05 and the coefficient value of Determination (R2) 80.11%. This means that approximately 80.11% of employee commitment and organizational culture factors have a significant influence on employee job performance. Partial test showed that the partial coefficient of determination values contained in the organizational culture variables for (r2) = 54.58% with p = 0.00000 and tresult = 7.515> ttable = 1.678 and ttable variables for employee commitment (r2) = 78, 74% with p = 0.00000 and tresult = 13.196> ttable = 1.678. These results indicate that organizational cultural factors and employee commitment have a significant relationship influence on employee job performance of the Riau Province Regional Secretariat (SetDa).
This study deals with the exploration of new sources of natural antioxidants as well as developing effective antioxidants. It is a collaborative project between two universities, i.e. University of Sindh, Jamshoro, Pakistan and St. Cloud State University, U.S.A.; concerning with (i) investigation of antioxidant potential of some herbs and spices in Pakistan (ii) exploring antioxidant activity of flavonoids and their metal complexes after the complexation. Among the food scientists, natural origin is focus of powerful research for exploitation of newer sources of antioxidants. In extension of the investigations, in the first phase of the study, a number of botanical materials have been selected for evaluation of their antioxidant probability. Antioxidant potential of different spices and herbs such as Ajwain, Cinnamon, Coriander, Cumin, Fennel, Fenugreek and Kalonji was investigated. The extracts of herbs and spices were prepared by electrical shaking and soxhlet extraction methods. A number of solvents ranging from polar to non- polar were used as extractant. Total phenolic content and antioxidant potentional by 2, 2-diphenyl-1- picryhydrazyl radical (DPPH·) were used as the parameters to study the effectiveness of extraction medium, i.e. solvent. Among the extracts of herbs and spices, ethanolic extract was found to be richer in phenolics and antioxidant activity. Moreover, total phenolic content, total flavonoid content, reducing power, chelating activity and DPPH· scavenging activity parameters were used for investigation of their antioxidant potential. From the results it has been deduced that most of the herbal spice materials exhibited appreciable antioxidant power as; compared to previously exploited sources of natural antioxidants. The Kalonji was found to be the richest source of antioxidants among the herbs and spices analyzed. The order of spices in antioxidant potential is Kalonji> Fenugreek> Cinnamon> Ajwain> Coriander> Cumin> Fennel. The flavonoids compose one of the most ubiquitous groups of all plant phenolics and they are an important class of defense antioxidants. The organoleptic properties and human health scientific studies have revealed their importance in food for maintaining human health. Flavonoids reduce the risk of stroke and heart disease, protect against relieve hay fever, age- related vision disorders, asthma symptoms, sinusitis, alleviate inflammatory skin conditions, reduce inflammation in joints and muscles, common to rheumatoid arthritis, minimize menopausal hot flushes, shrink hemorrhoids, reduce varicose veins and battle viral infections. Transition metal ions participate in the initiation of free radical processes. The antioxidant action of flavonoids has been considered to be via two possible modes of action, metal chelation and radical scavenging. Radical scavenging arises by donation of hydrogen atom from the free hydroxyls on the flavonoids nucleus and other possible antioxidant mechanism for flavonoids is metal chelation through phenolic OH groups, preventing metal- mediated generation of free radicals and, accordingly, may shelter the potential biological target from oxidative stress. However, in the second phase of study, among the most common dietary and biologically active flavonoids, Quercetin was selected. In order to study the chelation mechanisms of flavonoids with metal ions that permit a better understanding of their complexation and antioxidant properties; it has been decided to synthesize their metal (Mn Co 2+ , Ni 2+ , Cu 2+ 2+ , and Zn2+ ) complexes. This phase of study also consist on the characterization and investigation of antioxidant activity and the microbial activity of flavonoid-metal complexes. The thermal analyses as TGA and DSC. UV–Vis, IR, 1H-NMR spectroscopic technique were also used to asses relevant interactions of flavonoids and metal ions, the chelation sites are dependence upon the complex structure on the metal/ligand ratio, the capacity of flavonoids in binding metal ions, and etc. The outcome largely depends on the experimental condition and the type of assay employed. The Job''s method (continual variation method) was used to validate the stoichiometric composition of the chelate for the synthesis of metal flavonoid complexes. The complex formation reactions of M(II) Resulted in the formation of 2:1 metal:ligand complexes quercetin respectively. 70+ % yield have been found in the method employed for their synthesis. UV–vis spectroscopy remained the main tool for structural analysis of flavonoids. Both of the complexes formation and subsequent electron transfer reactions of M(II) with these ligands were examined by using UV–vis spectrophotometry. The 1H-NMR results indicate that the quercetin coordinate with two M(II) ion by replacing two of its protons. The coordination sites and the binding property of quercetin have also been evaluated by IR spectroscopy, which shows the shift of bands due to the formation of complex. The antioxidant and antimicrobial potentional of the complexes were studied and compared with the activity of free quercetin. The quercetin-M(II) complexes are found more potent free radical scavenger than the parent quercetin ligands and play a prominent role in protecting from oxidative stress and microbial organisms. This study demonstrates antioxidant potential of herbs and spices, and shows that the antioxidant activity of a flavonoid may be enhanced through chelation with metals.