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Carbohydrate Based Prebiotic Effects on Quality Attributes of Yoghurt and its Probiotic Endurance

Thesis Info

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Author

Hussain, Majid

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2180/1/2746S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725663511

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The research work was done to evaluate suitability of guar gum (CGG, PGG, AHGG, BHGG and EHGG) utilization as prebiotic on probiotic (BB) behavior in yogurt. CGG was purified (ethanol, 95:5v/v) and hydrolyzed using HCl, [Ba(OH)2] and mannanase enzyme. SEM characterization exhibited rough surface morphology in CGG, PGG and BHGG, powdery and fluffy appearance in AHGG and well defined porous structure in EHGG. The consequences of XRD displayed amorphous structure and less crystallinity in CGG, PGG and AHGG while EHGG slightly increased in crystallinity but lower than BHGG. FTIR analysis revealed no major transformation of functional groups after hydrolysis of guar gum. TGA evaluation of hydrolyzed guar gums indicated more heat stability than the crude ones. Rheological studies revealed shear thinning non-Newtonian behavior at high shear rate and Newtonian flow at low shear rate [high viscosity CGG (1.34 Pa.s); low viscosity AHGG (0.15 Pa.s), EHGG (0.22 Pa.s)] while, in oscillatory inferences (viscous modulus, Gʺ; elastic modulus, Gʹ), guar gums exhibited typical characteristics of weak viscoelastic gel. The small intestine model (SIM) evaluation exposed non-significant difference in physiological behavior of guar gums in reducing the glucose level. Haemolysis study showed non-toxic effect (haemolysis, 1.9±0.03% to 7.24±0.02%) of guar gums to human RBC’s. Guar gum treatments were evaluated for their prebiotic and probiotic endurance on yogurt quality attributes. Periodic physico-chemical, sensory characteristics and bacterial viability of the functional yogurt (Lactobacillus bulgaricus, Streptococcus thermophilus and Bifidobacterium bifidum + 0.1%, 0.5%, 1.0% of AHGG and EHGG) at 4-6°C revealed significant syneresis reduction, increase in WHC and viscosity exhibiting higher sensory acceptability of prebiotic and probiotic endurance. Cryo-scanning electron microscopy proved well embedded continuous casein network imparted by AHGG and EHGG granules. In the whole, acidity and syneresis increased but pH, viscosity, WHC, bacterial viability and consumer’s acceptability decreased with passage of time. The presence of guar gum as prebiotic also caused a significant effect on organic acids contents in yogurt due to metabolic activities of probiotic. The data obtained was analyzed using two-factor factorial design under CRD in Statistix 8.1 software. Key words: Guar gum hydrolysis, Characterization, Prebiotic, Probiotic, Functional Yogurt, Health benefits
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مولانا امین الدین شجاع الدین

امین الدین شجاع الدین
خبر آئی کہ مولانا امین الدین شجاع الدین بھی اپنے خالق و مالک حقیقی سے جاملے، یقین نہ آنے کے چند لمحوں کے بعد پھر اسی یقین کا اقرار کرنا پڑا کہ ہم سب اﷲ ہی کے ہیں تو واپس اسی کے جوار رحمت میں جانا ہی ہے۔
وہ ابھی ایسے نہ تھے کہ نام کے ساتھ مرحوم لکھا جائے، خدا جانے کتنی صلاحیتیں تھیں جو اب بھی ظہور کی منتظر تھیں، ان کا نام اچانک تعمیر حیات اور بانگ حرا کے صفحات پر دلکش، پر اثر اور البیلی تحریروں کے ساتھ سامنے آیا، ان کے اداریے نظر شوق کو متوجہ کرتے ، مقبولیت تھی کہ ان کے اداریوں کا ایک مجموعہ نقوش فکرو عمل کے نام سے مرتب ہوا، بھیونڈی کی زمین سے ندوہ کے آسمان تک کا سفر، تیز رفتار بھی رہا اور روشن بھی، کیا خبر تھی کہ یہ خوش درخشیدگی، شعلہ مستعجل کی مبتدا تھی، آخری ملاقات کب ہوئی یاد نہیں، لیکن ان کا تبسم اور محبت کی آنچ سے گداز ہاتھوں کا گرم جوش مصافحہ ضرور یاد ہے، وفیاتی مضامین کا مجموعہ’’ملنے کے نہیں نایاب ہیں ہمــ‘‘ عنایت کیا، ابھی معارف میں اس کے ذکر کی فرصت بھی نہیں ملی کہ وہ خود اس کتاب کا عنوان بن گئے، حضرت مولانا سید ابوالحسن علی ندویؒ سے سالک رام تک خدا جانے دل کی دنیا میں آ باد کیسے کیسے مکینوں کا نوحہ کہنے والے نگری نگری پھیرا لگاکر وہ اپنے سفر کی منزل پہنچ گئے، پس کارواں سخنوری کے ایسے نقوش قائم کرتے ہوئے جن کی چمک میں خون جگر کی آفرینش ہے، یہ نقوش تابند ہ رہیں گے اور کبھی کبھی دبے الفاظ میں یہ بھی کہہ جائیں گے کہ
کیا تیرا بگڑتا جو نہ مرتا کوئی دن اور
ندوہ کے ساتھ دارالمصنفین کے...

A Comparison of Bahaullah’s Religious Unity With Akbar’s Eclectic Din-E-Elāhi

Mirza Husayn Ali Nuri (1817-1892) was one of the early followers of the Bab, and later took the title of Bahaullah’s mission was about to bring unity of all the mankind. He invited the world’s religion followers to peaceful coexistence with amity and harmony. He claimed that he was unique, in giving the idea of ‘ Most Great Peace’ through ‘Religious unity’ and a ‘Global civilization’ as a chosen ‘Manifestation of God’. He claimed to be a messenger from God referring to the fulfillment of the eschatological expectations of Islam, Christianity, and other major religions. He wrote many religious works, most notably the Kitab i Aqdas, the Kitab i Iqan and Hidden Words. In the History of Sub-continent, Great Mughal emperor Jallal ud Din Mohammad Akbar (1542-1605) is also known for the great task of ‘Religious unity’. Disillusioned with orthodox Islam and perhaps hoping to bring about religious unity within his empire, Akbar promulgated Din i Ilahi, a syncretic creed derived from Islam, Hinduism, Zoroastrianism, and Christianity. Majority of muslims condemned him to deform the real shape of true Islam. Akbar was deeply interested in religious and philosophical matters. In 1575, he built a hall called the Ibadat Khana ("House of Worship") at Fatehpur Sikri, to which he invited theologians, mystics and selected courtiers renowned for their intellectual achievements and discussed matters of spirituality with them. The policy of sulh-e-kul, which formed the essence of D┘n-e-Elāhi, was adopted by Akbar not merely for religious purposes, but as a part of general imperial administrative policy. With the passage of time D┘n-e-Elāhi lost its attraction and became a dead religion. It is interesting to make a comparison between the two.

Production Potential and Economics of Maize Intercropped With Legumes As Influenced by Weed Control

Field trials were carried out at the New Developmental Farm of The University of Agriculture Peshawar Pakistan during the years 2012 and 2013 on intercropping of mungbean, sesbania and cowpea with maize crop. These trials were laid out in a Randomized Complete Block (RCB) design, with split-plot arrangement keeping three replications. In these experiments there were two main plots (herbicide vs no herbicide). The subplots comprised of 10 treatments including sole maize with 6 rows each 80 cm apart, sole mungbean with 15 rows each 30 cm apart, sole cowpea with 15 rows each 30 cm apart, sole sesbania with 15 rows each 30 cm apart, 5-rows-mungbean : 6-rows-maize, 5-rows-cowpea : 6-rows-maize, 5-rows-sesbania : 6-rows-maize, 10-rows-mungbean : 6-rows-maize, 10-rows-cowpea : 6-rows-maize and 10-rows-sesbania : 6-rows-maize. Maize was cultivated as the main crop and sesbania was intercropped as green manure, cowpea as fodder and mungbean as the grain purpose crops. The plots where herbicides were applied had significantly lower weed density, fresh and dry weed biomass during 2012 and 2013. The average weed density, fresh and dry weed biomass were lower in 2012 than in 2013. The weed density fresh and dry weed biomass were higher in sole maize plots among the intercropping treatments. The maize plant height was higher in plots of no herbicide use during both the years of study. It was lowest in sole maize plots in the two years study and highest in the plots of 10 rows mungbean intercropped with six row maize. The other maize parameters like number of grains ear-1, thousand grain weight, grain yield, biological yield, harvest index and maize grains protein content were higher in plots where herbicides were applied during both 2012 and 2013. Similarly, among the intercropping treatments, the number of grains ear-1, thousand grain weight, grain yield, biological yield, harvest index and maize grains protein content were highest in sole maize plots. Among the legume intercrops the performance of maize was best with cowpea followed by sesbania and mungbean. For mungbean crop, the number of seeds pod-1, thousand grain weight, grain yield, biological yield and harvest index were higher in herbicide applied plots in both of the years. The number of seeds pod-1, thousand grain weight, grain yield, biological yield and harvest index of mungbean were highest in the sole mungbean plots among the intercropping treatments in 2012 as well as in 2013. The grain yield, biological yield and harvest index of mungbean were lowest in the plots of 5 rows mungbean intercropped with 6 rows of maize in both the years’ trials. For the cowpea crop, the plant height and fresh weed biomass were significantly higher in plots of herbicide application during 2012 and 2013. Among the intercropping treatments, the plant height and fresh biomass of cowpea were highest in sole cowpea plots. For sesbania crop, the plant height, fresh and dry biomasses were significantly higher during 2012 and 2013 in plots where herbicides were applied. The plant heights, fresh and dry biomass of sesbania were highest in sole sesbania plots among the intercropping treatments in both of the trials conducted in 2012 and 2013. The total soil nitrogen and total organic matter were significantly higher during either years of study in herbicidal plots. Similarly, for the effect of intercropping the total soil nitrogen and organic matter were highest in sole mungbean plots during both years. The highest LER of 1.49 during 2012 was calculated for 10 rows sesbania intercropped with 6 rows maize. The LER trend in 2013 was similar to that in 2012. The cost benefit ratio ranged between 1.67 - 3.90 in the average yields of the two years in which the lowest CBR was computed for sole mungbean and the highest for 5 row mungbean intercropped with 6 rows maize. The aggressivity for all the intercropping treatments ranged between 0.1492 to 1.1545 with the minimum aggressivity in 5 rows mungbean intercropped with 6 rows maize and maximum in 10 row cowpea intercropped with 6 rows maize. The partial aggressivity for maize was higher than the legume intercrops in all the intercropping combinations. The competitive ratios for the intercropping treatments were in the range of 2.07-4.53 with the lowest competitive ratio for 5 rows sesbania intercropped with 6 rows maize and highest for 10 row cowpea intercropped with 6 rows maize. The partial competitive ratios for maize were also higher than the partial competitive ratio values for the intercrops in all the intercropping treatments. In conclusion, the use of herbicide in maize crop in integration with intercropping of legumes such as mungbean, cowpea or sesbania in alternate rows is a mean of achieving good weed control, improving soil fertility, attaining higher LERs, and often providing greater income to farmers.