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Characterization and Evaluation of Anti-Campylobacter Potential of Indigenous Lactobacilli in Poultry

Thesis Info

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Author

Khan, Madiha

Program

PhD

Institute

University of Veterinary and Animal Sciences

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Microbiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11844/1/MADIHA%20KHAN%20microbiology%202019%20uvas%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725675320

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IN VITRO CHARACTERIZATION OF PROBIOTIC PROPERTIES AND ANTICAMPYLOBACTER ACTIVITY OF LACTOBACILLI ISOLATED FROM POULTRY The aim of the present study was to isolate, characterize and develop indigenous probiotic lactobacilli targeting mitigation of Campylobacter jejuni. Lactobacilli (n=150) were isolated from poultry (62), fermented foods (78) and human faeces (10).All isolates were preliminarily screened for their anticampylobacter activity by well diffusion assay. Sixteen pre-selected isolates had activity against Campylobacter jejuni (n=4). Cell free supernatants (CFSs) of eight isolates (PL22, PL53, PL88, PL120, PL135, PL141, PL145 and PL149) retained activity after pH adjustment (6.5), while CFS of seven isolates (PL22, PL53, PL120, PL135, PL141, PL145 and PL149) retained activity after boiling at 80°C for 10 minutes. Selected isolates were further characterized for their tolerance to low pH and bile salts, antibiotic susceptibility, auto-aggregation and co-aggregation properties. All selected isolates had significant tolerance to low pH (>50% survival at pH 3), bile salts (0.3%), autoaggregation and coaggregation properties. Selected isolates were safe and had no acquired resistance to penicillin, tetracycline, ampicillin and erythromycin. PL53, PL120 and PL149 showed significantly higher reduction (> 3 log reduction) of Campylobacter jejuni ATCC 33291 in broth culture. It is concluded that Lactobacillus gallinarum PL 53, Lactobacillus casei PL 120 and Lactobacillus gallinarum PL 149 had probiotic potential and anti-campylobacter properties.Keywords: probiotics, Lactobacillus, Campylobacter, pH tolerance, antimicrobial activity EFFECT OF NEWLY CHARACTERIZED PROBIOTIC LACTOBACILLI ON WEIGHT GAIN, IMMUNOMODULATION AND GUT MICROBIOTA OF CAMPYLOBACTER JEJUNI CHALLENGED BROILER CHICKEN The aim of the present study was to determine the competitive exclusion of Campylobacter jejuni inpoultry gut by three potential probiotic lactobacilli strains. A total of 135 birds of one day age were randomly divided into nine groups. The groups of prevention model received respective strains from day 1-35 and treatment model received lactobacilli from day 15-35 (after challenging with C. jejuni). These groups were given lactobacilli (~108 CFUs) while challenged with C. jejuni on day 14 by oral gavage (106 CFUs/bird). There were three control groups including A, B and I. Negative control (A) did not receive any treatment, C. jejuni was given to group B and group I was given Enrofloxacin formulation. Cloacal swabs were collected from birds of each group before and after challenge while the ceca were collected from birds after slaughtering (on day 35) for enumeration of aerobic bacteria, coliform, C. jejuni, lactobacilli and Bifidobacterium on selective agar plates. Effect of lactobacilli on weight gain and New Castle Disease vaccine (NDV) titer were also evaluated. Probiotic strain Lactobacillus gallinarum PL 53 considerably decreased log10 values of aerobic plate count (3.19±0.66), coliform count (2.83±0.22) and C. jejuni (3.98±0.77) in poultry. The probiotics also enhanced Lactobacillus and Bifidobacterium spp. counts (~2-3 log increase). Treatment group C had maximum weight gain (1994±188.32g) and geometric mean titer (274.4) on day 28. The results of in-vivo experiments concluded that probiotic administration may be effective for targeted mitigation of C. jejuni in broiler birds.
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کس زباں سے ہم بتائیں ہم کو کیا اُنؐ سے ملا


کس زباں سے ہم بتائیں ہم کو کیا اُنؐ سے ملا
منزلِ عرفانِ حق کا راستا اُنؐ سے ملا

نوریوں پہ نورِ صبحِ کُن فکاںؐ کی بارشیں
پیکرِ خاکی کو حسنِ دلربا اُنؐ سے ملا

لمسِ نعلینِ نبیؐ سے جن کو تابانی ملی
کہکشائوں کا حسیں تر سلسلہ اُن سے ملا

کس قدر خوش بخت ہے حسانؓ بن ثابت کی ذات
نعت کہنے ، پڑھنے ، سننے کا صلہ اُنؐ سے ملا

وادیِ طائف میں صبر و استقامت دیکھ کر
عزم و ہمت کا سبق ہم کو جدا اُنؐ سے ملا

اُنؐ کو خالق نے بنایا ، قاسمِؐ انعامِ کُل
جو ملا ، جب بھی ملا ، جتنا ملا ، اُنؐ سے ملا

جب بھی دی عرفانؔ نے دہلیزِ اقدس پر صدا
صدقۂ آلِ نبیؐ اُس کو سدا اُنؐ سے ملا

Admissibility of a Video As Evidence in a Court: its Presentation and Probative Value

To general public, all videos are perceived to be true, but they may not have probative value in the Court of law. The undertaken article analyzes the admissibility and probative value of a video presented as evidence before a court in the Criminal Justice System of Pakistan (CJSP). It analyzes the relevant law and diagnoses the problems with the video evidence through the lens of the judgments of Superior Courts. The court of law objectively ascertains that a video presented as evidentiary means bears significant relevance to the fact in question. It must be admissible under the law, and it must be proved to be genuine. To fill up the gap between a “Video” and a “Video Evidence”, there is a process, which is known as video authentication. It determines that the video contents are genuine, authentic, credible, unaltered, untampered and unfabricated. The study discusses various modes of video authentication. Precedents set by superior courts of Pakistan show that convictions have been made once the courts are satisfied with the credibility of video evidence. In the court of law, video evidence is normally presented after the completion of prosecution evidence. The video is played in court and is watched by the presence. But the researcher establishes that such process does not have legal justification. The article suggests that it would be legal and proper for the prosecution to produce the video evidence through the witness, during his evidence, who is either victim, witness, recorded and/or copied the video directly from original source such as C.C.T.V system and that witness would be subjected to cross examination.

Transfusion Transmitted Viral Infections in High Risk Groups of Khyber Pakhtunkhwa, Pakistan

Introduction Blood transfusion is a specialized modality of patient management to sustain health and life, but carries a definite risk of acquisition of transfusion transmitted virus (TTV) infections particularly in resource limited settings. TTV infections are of great concern because of their asymptomatic nature, protracted viremia, highly variable course and of fatal, chronic and life threating disorders. The prevalence of TTVs among the blood recipients reflects the disease burden among the blood donors and general population. Material and method The study population was categorized in two groups on the basis of the history of blood transfusion: individuals with no history of blood transfusion were categorized as control group and individuals with history of blood transfusion were categorized as high risk group. High risk group was further divided into four sub groups; thalassemia, hemophilia, hemodialysis, and surgical patients. Sera of all individuals were investigated for the presence of HBsAg, anti-HCV and anti-HIV by using immune-chromatigraphic tests, followed by molecular analysis by PCR for the detection of viral DNA and RNA and genotypes. Result The study population consists of 4,607 individuals, 2,032 individuals comprised control group and 2,575 individuals comprised high risk group. Overall, 21.12% (973) individuals showed the evidence of TTV, among them 3.6% (166) was positive for HBsAg, 17.39% (801) was positive for anti-HCV and 0.13% (6) was positive to HBV&HCV while no individual was positive to HIV. 75 individuals were positive to HBV DNA, 18 individuals (27.69%) were classified into genotype B, 11 (16.92%) genotype C, 35 (53.84%) genotype D and 1 (1.54%) genotype F, 10 xi (15.38%) untypeable. 366 individuals were positive to HCV RNA, HCV genotypes 1 was detected in 71 (20.9%) individuals, genotype 2 in 17 (5.01%), genotype 3 in 182 (53.69%), genotype 4 in 16 (4.72%), genotype 5 and genotype 6 in 16 (4.72%), mixed genotype in 25 (7.37%) and untypeable in 32 (0.69%). In control group, 77 (3.7%) individuals was confirmed positive for TTV. Total 0.59% (12) individuals were positive for HBsAg, of which 58.33% (7) were male and 41.7% (5) were female, while HBV DNA was positive in 91.66% (11) individuals. Anti-HCV was positive in 3.2% (65) individuals, of which 58.46% (38) were male and 41.54% (27) were female, while HCV RNA was positive in 69.23% (45) individuals. In high risk group, 973 (4.8%) individuals was confirmed positive for TTV. 3.6% (166) individuals were positive for HBsAg, 64.93% males and 35.06% females, while HBV DNA was positive in 1.6% (75) individuals. Anti-HCV was positive in 17.39% (801) individuals, 18.83% were males and 12.73% were female, HCV RNA was positive in 7.94% (366) individuals. Dual infection was detected in 0.13% (6) individuals. HBsAg were detected in 11.86% (58) thalassemia patients, 11.54% (42) hemophilia patients, 2.75% (22) in patients undergoing hemodialysis and 3.47% (32) of patients with surgery. Anti-HCV were detected in 29.24% (143) thalassemia patients, 43.96% (160) hemophilia patients, 41.93% (335) of patients undergoing hemodialysis and 10.62% (98) of patients with surgery. Co-infection with HBV and HCV was encountered in 0.63% (5) of patients undergoing hemodialysis and 0.11% (1) of patients with surgery. Statistical significance was observed for TTVs between thalassemia patients and hemophilia patients (χ2=24.31, p<0.00001), thalassemia patients and xii surgical patients (χ2= 69.174, p<0.00001), surgical patients and HD, (χ2= 127.594, p<0.00001), surgical patients and hemophilia patients (χ2= 96.835, p<0.0001) and HD and hemophilia patients (χ2= 6.158, p=0.013082). However, the difference between thalassemia patients and HD (χ2= 2.173, p=0.14045) was not statistically significant. Conclusion The present study critically evaluated prevalence of three major transfusion-associated infections, namely infections by HIV, HCV, and HBV. The present investigation showed i. A substantial percentage of the individuals harbored TTV infections (21.21%). ii. The prevalence of TTV was high (34.8%) in high risk group in comparison to the control group (3.7%) and thus ranked at high risk for TTVs infection. TTV Infection prevalence was 9.41 folds higher in high risk group than in control group. It showed that TTVs continue to endanger safe blood supply in a country. iii. The frequency of HCV was high (17.39%) in both high risk group and control group in comparison to HBV positivity (3.6%), the situation is alarmingly and is a matter of concern and effort. iv. The prevalence of untypeable genotypes of HBV and HCV was alarming v. TTV positivity rate increase with increase in the age in high risk group as their transfusion requirements increased with age, thus they were more prone to these infections. vi. The prevalence of mixed genotypes of HCV was matter of concern. vii. This burden of TTV showed that prevention of spread of TTV should be the main goal at the current time. xiii viii. The over flaws in blood transfusion services can be overcome by development of a fair and organized system for safe blood screening, monitoring the implementation strategies for recruitment and retention of safe donors. Recommendation The occurrence of TTV infections should still be monitored carefully to reduce the rate of infections to ensure safer and more reliable blood for transfusion. Education regarding awareness about TTVs, screening strategies, sensitization and vaccination must be carried out to ensure that people are well enlightened and protected from viral diseases. All individuals particularly patients receiving blood transfusion should complete vaccination for Hepatitis B before starting transfusion. Strict and concrete efforts are required to cut down the infection rate through proper screening of blood and blood products, strict emphasis on receiving the vaccine, uniform strict criteria for donor selection.