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Home > Chemical Synthesis, Characterization and Biomedical Applications of Metallic Nanoparticles

Chemical Synthesis, Characterization and Biomedical Applications of Metallic Nanoparticles

Thesis Info

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Author

Zafar, Nosheen

Program

PhD

Institute

University of Engineering and Technology

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Physics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9556/1/Nosheen_Zafar_Physics_HSR_2016_UET_lahore_22.06.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725714468

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Metallic nanoparticles have potential applications in the field of biomedical and nanobiotechnology due to their enhanced antimicrobial characteristics. In present research an optimum experimentation for the fabrication of metallic (Ag, Au, Cu and Ni) nanoparticles via chemical reduction method was investigated. For all metallic nanoparticles same reducing agent Sodium Borohydride and Trisodium citrate was used with two stabilizers Sodium Dodecyl Sulfate (SDS) and Polyvinylpyrrolidone (PVP) except Ni nanoparticles where hydrazine was utilized. All particles were characterized by AFM, XRD, FTIR and UV-VIS spectroscopy. Fabricated nanoparticles Ag, Au, Cu and Ni NPs has size ranges 1-100 nm, 2-40 nm, 50-180 nm and 7-180 nm respectively. Sharp and prominent peaks of all metallic nanoparticles were investigated via optical characterization. Structural exploration of all metallic NPs were revealed poly crystalline structure with face centered cubic (FCC) having prominent [111] index, insuring their performance as an efficient antibacterial toll. FTIR evaluation of all metallic NPs verified their pure metallic nature. Antibacterial behavior of all particles was evaluated by turbidity (liquid media) and disk diffusion (solid media) method. It was deduced that Silver NPs (30-40 nm) exhibited Maximum Inhibition calculations (MIC) ~ 2.8, 4.37, 13.5 and 2.81 μg/ml for E. coli, S. aureus, B. subtilis and Salmonella. Similarly Silver NPs (20-30 nm) had MIC calculations of ~ 2.10, 2.36 and 2.68 μg/ml for E. coli, S. aureus and Salmonella. High dose of Silver NPs (30-40 nm) exhibited maximum zone diameter ~ 21 and 13 mm for E. coli and S. aureus while Silver NPs (20-30 nm) demonstrated zone diameter of ~ 23 and 15 mm for E. coli and S. aureus respectively. Gold NPs (7-34 nm) exhibited MIC value ~ 2.93, 3.92, 3.15 and 7.56 μg/ml for E. coli, S. aureus, K. pneumonia and B. subtilis respectively. Similarly MIC values deducted from Gold NPs (20-40 nm) were 2.96, 3.98, 3.3 and 8.61 μg/ml for E. coli, S. aureus, K. pneumonia and B. subtilis respectively. High dose of Gold NPs (7–34 nm) exhibited maximum zone diameter ~ 25 and 35mm for S. aureus and E. coli while Gold NPs (20- 40 nm) high dose demonstrated the zone ~ 22 and 31mm for S. aureus and E. coli respectively. Copper NPs with MCE filtration (10 - 150 nm) had MIC values ~ 6.07 and 8.33 μM for E. coli and S. aureus respectively. Similarly Cu NPs without MCE filtration (5-250 nm) had MIC values ~ 9.32 and 11.76 μM for E. coli and S. aureus respectively. Again high dose of Copper NPs (10-150 nm) demonstrated the zone diameter ~ 32 and 22 mm for E. coli and S. aureus respectively. Nickel nanoparticles (7-65 nm) revealed MIC calculation ~ 34.3 and 45 μM against E. coli v and S. aureus respectively and the zone calculation for S. aureus and E. coli was ~ 19 and 21 mm respectively with 30 μM dose. 5FU drug was loaded on Gold NPs (20-65 nm) successfully and zone of inhibition for E. coli and S. aureus was calculated. It was revealed that 15 μg/ml dose of 5FU coated Au NPs is ~ 40 mm for S. aureus, 31 mm for Salmonella and 44 mm for E. coli which was much enhanced as compared to pure Au NPs and pure 5FU drug. Gold NPs first time used in parthenogenesis activation process of mouse oocytes as a catalyst. 250 nmol Gold NPs dose with constant SrCl2 concentration (10 mM) (for 3 hour exposure) is proved to be suitable for oocytes activation of mouse.
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A series of field experiments were conducted to evaluate the integrated application of organic and inorganic nutrient source, cropping systems and different tillage methods under irrigated and non-irrigated conditions and study their effects on soil physiochemical properties, soil fertility and crop productivity. The experiments were carried out at Agricultural Research Station Serai Naurang, Bannu, Pakistan. Tillage methods included deep (DT) and conventional shallow tillage (CT) while cropping system consisted of wheat-maize-wheat, wheat-mungbean-wheat under irrigated condition and wheat-mungbean-wheat, wheat-fallow-wheat under non-irrigated condition. The fertilizers treatments were farmer’s practice T1 (50 % NP), balance inorganic fertilizers T2 (100 % NPK recommended dose i.e. 120, 90 and 60 kg ha-1), integrated inorganic and organic fertilizers T3 (50 % N + 100 % PK + 10 tons FYM ha-1) and T4 (25 % N + 100 % PK + 20 tons FYM ha-1). The experimental design was RCB with split-split plot arrangement having three replications. Tillage was allotted to main plots, cropping system to subplots and fertilizer treatments to sub-subplots. Deep tillage treatment resulted higher soil available water holding capacity, mineral N and lower soil bulk density than CT under both the conditions. Higher wheat straw, grain and total NPK uptakes was observed in DT than CT. The straw and grain yield of wheat significantly increased with deeply ploughed plots under both irrigated and non-irrigated conditions. However, soil organic matter (OM), AB-DTPA extractable P and K, pH, microbial biomass and population was not affected by any tillage methods. Mungbean-wheat cropping system had performed better than maize-wheat or fallow-wheat. Greater soil OM, mineral N, N uptake, microbial biomass and microbial population was recorded in mungbean-wheat rotation than fallow-wheat under non-irrigated condition, whereas higher wheat straw and grain yield was recorded in mungbean-wheat compared to maize-wheat rotation under irrigated condition. Similarly, incorporation of FYM either 10 tons with 50 % N + 100 % PK (T3) or 20 tons with 25 % N + 100 % PK (T4) had increased soil available water holding capacity, organic matter, mineral N, AB-DTPA extractable P and K, microbial biomass and population but decreased soil bulk density under both conditions. Greater straw N, P and K contents were measured in plots having 50 % N + 100 % PK + 10 tons FYM ha-1 and 25 % N + 100 % PK + 20 tons FYM ha-1 compared to the plots receiving sole mineral fertilizers, whereas grain N was increased in plots receiving balance inorganic fertilizers under both the conditions. Integrated application of 50 % N + 100 % PK with 10 tons FYM ha-1 had improved straw, grain and total N, P and K uptakes compared to other treatments under each conditions. Fertilization of 50 % N + 100 % PK with 10 tons FYM ha-1 had further improved wheat grain yield with 56 % increase under irrigated condition and 49 % increase over farmer’s practice under non-irrigated condition followed by the plots receiving sole recommended 100 % NPK. The summer crops of maize and mungbean responded significantly to combine application of organic and inorganic fertilizers. Addition of 50 % N + 100 % PK + 10 tons FYM ha-1 had increased maize grain and stover yield compared to the other treatments under irrigated condition. Plots incorporated with 50 % N + 100 % PK + 10 tons FYM ha-1 or 25 % N + 100 % PK + 20 tons FYM ha-1 produced comparable yield of mungbean, but significantly higher from plots having sole inorganic fertilizers under both the conditions. Integrated use of 50 % N + 100 % PK + 10 tons FYM ha-1 with DT under wheat-mungbean-wheat cropping system had improved crop productivity and soil fertility beside soil health and environmental benefits and thus recommended for irrigated and non-irrigated wheat sowing in agro-climatic condition of Bannu, Pakistan.