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Home > Common Fusion Oncogenes in Pediatric Acute Lymphoblastic Leukemia and Their Role in Differential Diagnosis and Prognosis

Common Fusion Oncogenes in Pediatric Acute Lymphoblastic Leukemia and Their Role in Differential Diagnosis and Prognosis

Thesis Info

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External Link

Author

Tashfeen Khalid Awan

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2446/1/3039S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725747809

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Acute lymphoblastic leukemia (ALL) is a complex genetic disease involving many fusion oncogenes having prognostic significance. The frequency of various fusion oncogenes can vary in different ethnic groups, with important implications for prognosis, drug selection and treatment outcome. We studied fusion oncogenes in 101 pediatric ALL patients using RT-PCR and interphase FISH, and their associations with clinical features and treatment outcome. Five most common fusion genes i.e. BCR-ABL t (22; 9), ETV6-RUNX1 (t 12; 21), MLL-AF4 (t 4; 11) TCF3-PBX1 (t 1; 19), and SIL-TAL1 (del 1p32) were found in 88.1% (89/101) patients. Frequency of BCR-ABL was 44.5% (45/101). BCR-ABL positive patients had a significantly lower survival (43.7±4.24 weeks) and higher white cell count as compared to others, except patients with MLL-AF4. The highest relapse-free survival was documented with ETV6-RUNX1 (14.2 months) followed closely by those cases in which no gene was detected (13.100). RFS with BCR-ABL, MLL-AF4, SIL-TAL1 and TCF3-PBX1 was less than 10 months (8.0, 3.6, 5.5 and 8.1 months, respectively). This is the first study from Pakistan correlating molecular markers with disease biology and treatment outcome in pediatric ALL. It revealed the highest reported frequency of BCR-ABL fusion gene in pediatric ALL, associated with poor overall survival. Present data indicated an immediate need for incorporation of tyrosine kinase inhibitors in the treatment of BCR-ABL+ pediatric ALL in this population and the development of facilities for stem cell transplantation.
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سیالکوٹ میں اردو غزل اقبال سے صابر ظفر تک

سیالکوٹ میں اردوغزل (اقبال سے صابر ظفر تک)

علامہ اقبالؒ کی شاعری کا ایک بڑا حصہ غزلیہ اشعار پر مشتمل ہے۔بانگِ درا،بالِ جبریل  اور ضربِ کلیم شعری مجموعوں میں اقبالؒ کی کثیر تعداد میں غزلیں ملاحظہ کی جا سکتی ہیں۔ان میں کچھ مسلسل غزلیں بھی  ہیں جن میں ایک ہی طرح کے مضامین ملتے ہیں۔اقبالؒ داغ دہلوی کے شاگرد تھے ان کی ابتدائی شاعری پر داغ کے اثرات دیکھے جا سکتے ہیں۔"باقیاتِ اقبال "کے نام سے مختلف محققین نے اقبالؒ کا جو منسوخ کلام  مرتب  کیا ہے ان پر بھی داغ کا رنگ واضح نظر آتا ہے۔بانگ ِ درا کی کچھ غزلوں میں بھی داغ کا اندازِ بیان دیکھا جا سکتا ہے۔اگرچہ اقبالؒ کی غزل کے مضامین کلاسیکل اور روایتی شاعری سے منفرد ہیں لیکن کہیں کہیں کلاسیکل اور روایتی رنگ دیکھا جا سکتا ہے۔اس حوالے سے کلام ِ اقبال ملاحظہ ہو:
مانا کہ تیری دید کے قابل  نہیں ہوں میں
تو میرا شوق دیکھ میرا انتظار دیکھ
کھولی ہیں ذوقِ دید نے آنکھیں تری اگر
ہر رہگزر میں نقش کف پائے یار دیکھ1
نہ آتے ہمیں اس میں تکرار کیا تھی
مگر وعدہ کرتے ہوئے عار کیا تھی
تمہارے پیامی نے سب راز کھولا
خطا اس میں بندے کی سرکار کیا تھی
بھری بزم میں اپنے عاشق کو تاڑا
تری آنکھ مستی میں ہشیار کیا تھی
تامل تو تھا ان کو آنے میں قاصد
مگر یہ بتا طرزِ انکار کیا تھی2
موت کا نسخہ ابھی باقی ہے اے درد فراق
چارہ گر دیوانہ ہے ،میں لادوا کیونکر ہوا
میرے مٹنے کا تماشا دیکھنے کی چیز تھی
کیا بتاؤں ان کا میرا سامنا کیونکر ہوا3
انوکھی وضع ہے سارے زمانے سے نرالے ہیں
یہ عاشق کو ن سی بستی کے یا رب !رہنے والے ہیں
رلاتی ہے مجھے راتوں کو خاموشی ستاروں کی
نرالا عشق ہے میرا...

رویہ صارف (Consumer Behavior) اور اسلامی اخلاقیات

Consumer Behavior means behavior of consumers about buying goods and services in the market. Consumer behavior entails all activities associated with the purchase, use and disposal of goods and services, including the consumer emotional, mental and behavioral responses that precede or follow these activities. The following article discusses consumer behavior in the light of Islamic ethics, and explains different aspects of consumer behavior according to Islamic moral values and also given suggestions to consumers and marketers to adopt Islamic buying and selling behavior, which is beneficial not only for individual but also to human as a whole. The consumer behavior in the modern capitalist economy plays very important role, not only in consumption but also in the production and services.

Antimicrobial, Antioxidant and Protective Efficacy of Flower and Leaf Extracts of Calotropis Procera Against Free Radical Damage

Different soluble fractions viz., hexane, ethyl acetate, butanol and ethanol of Calotropis procera (Ait.) R. Br. were screened for their antimicrobial properties by using agar-well diffusion method against the human pathogens viz., Escherichia coli and Salmonella typhi (Gram negative), methcillin resistant Staphylococcus aureus and Micrococcus luteus (Gram positive), in vitro antioxidant properties were analyzed by means of DPPH free radical scavenging method, reducing ability assay and lipid peroxidation inhibition method. Furthermore, in vivo protective efficacy of C. procera extract against (NSAID) ibuprofen-induced nephrotoxicity in rat model was also determined by evaluating renal function markers, plasma measure of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) along with the determination of tissue lipid peroxidation markers, i.e. aldehyde products malonyldialdehyde (MDA) and 4- hydroxy-nonenal (4-HNE). Phytochemical analysis was also carried out for the detection of phenolic constituents, amino acids, protein, carbohydrates, reducing and non-reducing sugars in test plant. In the present findings the hexane fraction of C. procera flower and leaf have been proved very significant with maximum zones of inhibition i.e., flower (22mm) and leaf (23mm) against M. luteus. While, other tested fractions of C. procera flower and leaf showed significant antimicrobial activity against all pathogens. Whereas, in the present finding it was also determined that the flower ethanol extract showed the highest DPPH free radical scavenging activity (88.19% with 8 mg/ml) as compared to BHA which showed 85% scavenging activity as standard. Similarly, C. procera flower and leaf extracts were also analyzed for reducing capacity. The highest absorbance (i.e., 1.827 with 10mg/ml) was recorded in C. procera flower water extract as compared to standard which showed (0.238) absorbance. In vitro lipid peroxidation inhibition, another model was used to check the antioxidant capacity of C. procera. Flower water extract exhibited a concentration dependent increase in lipid peroxidation inhibition, the highest value is (89.58% with 10mg/ml) while the lipid peroxidation value in C. procera leaf water extract (i.e., 75.11% with 10mg/ml) and leaf ethyl acetate extract showed (75.11% with 8mg/ml). While, BHA (85%) and ascorbic acid (75.5%) showed lower values as compared to tested plant. However, body weight loss was successfully restored by the coadministration of Ibuprofen with C. procera hexane extract. While, increased level of renal function markers (urea, creatinine) was normalized by the administration of C. procera hexane with ibuprofen treatment. The imbalance in oxidative status was determined by evaluating decreased level of catalase, superoxide dismutase and glutathione along with increased levels of malonyldialdehyde and 4- hydroxynonenal, which was counteracted by the co-administration of C. procera hexane extract with ibuprofen which maintained cell sustainability and indicated nephro-protective activity of C. procera. Besides the above results C. procera leaf and flower aqueous extract were also used to check enzymatic activities of glucoamylase, α-amylase and urease enzymes. The flower extract is found proved to be a good enhancer of glucoamylase, α-amylase and urease activity as compared to leaf extract. A number of phytoconstituents were also detected. The presence of phytochemicals in C. procera may indicate a good correlation with that of antibacterial, antioxidant potential and protective role for in vivo model which also proved as a good enhancer of enzyme activities. Thus due to aforementioned activities, Calotropis procera may serve as a better and a protective therapeutic agent than any other synthetic drug.