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Home > Comparative Efficacy of Different Maize Zea Mays L . Genotypes for Acquistion and Utilization of Iron and Zinc under Saline Conditions

Comparative Efficacy of Different Maize Zea Mays L . Genotypes for Acquistion and Utilization of Iron and Zinc under Saline Conditions

Thesis Info

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External Link

Author

Batool, Munaza

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Soil Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/10475

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725755533

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This study was designed to examine the relationship of classroom learning environment and Teacher Interaction with secondary school students’ attitude towards the learning of English Language in context of Pakistan. Classroom environment is considered as one of the influential factor in enhancing learners’ achievement, attitude and learning process. The objectives of the study were to investigate secondary level students’ English language classroom learning environment, teachers’ interaction in English language classes and attitude towards learning of English language. Moreover, the relationship of classroom learning environment and teacher interaction with secondary level students’ attitude towards the learning of English on gender and locale was also established in the study. The study at hand was co relational and descriptive. For data collection survey method was used. Multistage stratified random sampling technique was used for sample selection of the study. Stratification was based on three geographic zones of Punjab: Northern, Central and Southern Punjab. One district from each zone was selected randomly. The sample of the study was 930 students from 03 districts of the Punjab. Three instruments were used, first for Classroom learning environment, second for discovering Teacher Interaction in English language classes and third for finding out students’ attitude towards learning of English language. Data for this study were analyzed by using statistical operations through SPSS. T-test was used to calculate the significant difference between means of different categories of respondents like rural & urban, male and female. To investigate the possible relationship among the variables, Pearson correlation was used. Results of the study showed that male and female respondents reflected significant differences on English language classroom learning environment, teacher interaction and attitude towards English. It further revealed that female respondents were more favorable on their classroom learning environment, get more teachers’ support and interaction than male in their English language classes and had more positive attitude towards the learning of English. Significant positive correlation was found among classroom learning environment, Teacher Interaction and attitude towards the learning of English. Findings suggest that positive attitude in a foreign language class can be developed by making learning environment more favorable in shape of better pupil-teacher relationships and teacher support, involvement, cooperation and equity. It may help in developing attitude towards Adoption of English language learning attitude and enjoyment of English lessons in the classroom.
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Biotechnological Approaches for Enhanced Production of Biosurfactantants by Bacillus Subtilis Snw3

Biological surface-active agents or “biosurfactants” are the compounds that can reduce the surface or interfacial tension between two same or different phases (liquid, gas and solid). The present study relates to the screening of biosurfactant-producing bacteria isolated from the Fimkassar oil field, Chakwal, Pakistan. The molecular screening for two important genes srfA and rhlB responsible for production of surfactin and rhamnolipid biosurfactants, respectively and biosurfactant production by using different growth substrates. In total, 38 out of 70 different bacterial isolates showing growth on crude-oil-containing media were screened for biosurfactant production. Evidently, 34.2% (n = 13) of the isolates were found to have the srfA gene, while 15.8% (n = 6) of the isolates contained the rhlB gene. 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Lowering the C:N ratio by providing nitrogen by addition of red bean and urea has a profound effect on biosurfactant production especially using RB+PS+U (6+0.5+0.4%) in the medium resulting in 1.2 g/L surfactin and 300 mg/L fengycin. Optimization studies of temperature, agitation speed, inoculum size and age of culture revealed maximum production of surfactin (1.37 g/L) and fengycin (700 mg/L) at 23 °C (room temperature), 120 rpm, 2 % inoculum of 36 hours old culture by using the combination RB+PS+U (6+0.5+0.4%). Heat treatment (autoclavation) was found to havea positive effect on extraction of amino acids and sugars that led to ahigher amount of surfactin and fengycin production as compared to the extract of red bean that was prepared directly. Red bean extract (prepared by autoclavation) produced 792 mg/L surfactin and 546 mg/L fengycin, while 329 mg/L surfactin and 197 mg/L fengycin was produced by red bean extract. Batch experiments were performed in a 13-L bioreactor. Maximum production of surfactin 1512 mg/L and 1236 mg/L fengycin (surfactin +fengycin) (named VITO Surf) was observed at the 7th day ofincubation by Bacillus subtilis SNW3 at 23 °C pH 6.8 and 120 rpm. Biosurfactant production was found to be improved by using mutant M-20 and M-40 (Mutagenesis was performed by UV treatment) with reduced incubation time. LC/MS showed very interesting results that M-20 produced 1000 mg/L surfactin and M-40 produced 824 mg/L surfactin after 26 hours of incubation and immediately the concentration of surfactin decreased while the parent strain could produce about 300-400 mg/L at the same time of incubation. Similarly both mutants produced only surfactin. Providing an increased amount of red bean powder (100 mg/L) in the culture medium of both mutants, M-20 and M-40, resulted in an increased amount (1507 mg/L) of surfactin. Fed batch fermentation was performed to check addition of red bean powder and urea during fermentation using the mutants. 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