Search or add a thesis

Advanced Search (Beta)
Home > Comparative Efficacy of Single and Multi-Strain Bacterial Inculation for Improving Growth and Yield of Wheat under Salinity Stress

Comparative Efficacy of Single and Multi-Strain Bacterial Inculation for Improving Growth and Yield of Wheat under Salinity Stress

Thesis Info

Access Option

External Link

Author

Khan, Muhammad Yahya

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Soil Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13578/1/Muhammad_Yahya_Khan_Soil_Science_2016_UAF_13.02.2017.doc

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725755856

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Soil salinity is one of the worst consequences of global climate change, which is declining the world agriculture production. Pakistan is more vulnerable to climate variability where increasing soil salinity and drought are alarming for agriculture produce. Researchers are devoting attention for the use of microbial strategies to improve plant tolerance against abiotic stresses under extreme environment because of their ecological benefits. Plant growth promoting rhizobacteria (PGPR) induce salinity tolerance in plants with their multiple mechanisms. Despite the fact that bacterial inoculation improves the plant growth but their field application is limited due to inconsistency of results and some environmental constraints. However, efficiency of bacterial inocula for plant growth promotion may be increased under field conditions by employing bacterial consortia instead of using single strain inoculation. Therefore, present study was conducted to evaluate comparative effectiveness of single, dual and triple strain inoculation of salt tolerant PGPR for improving growth and yield of wheat under salt affected conditions. Twenty five rhizobacteria having 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity were isolated from wheat rhizosphere and were tested for their salt tolerance as well as plant growth promotion potential under gnotobiotic conditions. Out of 25 rhizobacterial isolates, 7 isolates were statistically significant for improving growth of wheat under saline conditions. These 7 salt tolerant PGPR were tested for their compatibility of growth and synergism with each other. Out of 7, only 3 isolate found synergistic and showed abilities to coexist. On the basis of rrs (16S rRNA) gene sequencing, these 3 PGPR were identified as Bacillus cereus strain Y5, Bacillus sp. Y14 and Bacillus subtilis strain Y16. Selected PGPR strains showed variable ACC-deaminase activity, P-solubilization, exopolysaccharides production, biofilm formation and auxins biosynthesis, when used alone as well as in their dual and multi-strain combination for different plant growth promoting activities under normal and salt stress conditions. Growth pouch experiment under axenic conditions was conducted to evaluate selected PGPR for inducing salinity tolerance in wheat as single and multi-strain inoculation. In growth pouch assay, multi-strain inoculation increased the total dry weight of wheat up to 28, 54, 59 and 56% at 0, 50, 100 and 150 mM of NaCl salinity levels, respectively, as compared to respective un-inoculated control at same level of salinity. Furthermore, pot and field experiments were conducted in salt affected soils to confirm the consistency of results for plant growth promotion by selected PGPR alone as well as in their possible combinations. In pot experiment, maximum increases in number of tillers were observed with multi-stain inoculation which were up to 54 and 80% more as compared to respective un-inoculated control at 10 and 15 dS m-1 salinity levels, respectively. Multi-strain inoculation also increased the grain yield by 18, 20, 42 and 62 at 1.42 (original), 5, 10 and 15 dS m-1 salinity levels, respectively, as compared to respective un-inoculated control. Further, single and multi-strain inoculations showed positive effect on all growth and physiological attributes of wheat under salt affected field conditions. However, the response of single, dual and triple strain inoculation treatments was highly variable at selected four sites. Maximum increases in grain yield of wheat at field-I, field-II, field-III and field-IV were up to 17, 17, 23 and 26%, respectively, over un-inoculated control by multi-strain inoculation of Y5*Y14*Y16. It might be concluded from this study that multi-strain inoculation with PGPR containing ACC-deaminase could be very promising option for inducing salinity tolerance in wheat, most probably due to better root growth in salinity stress which might be attributed to elimination of root inhibiting factor like production of stress induced ethylene.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

مولاناابوالوفاء افغانی

مولانا ابو الوفاء افغانی
معارف کے قارئین مولانا ابوالوفاء افغانی کے نام سے اور کام سے بخوبی واقف ہیں ان کی پیدائش افغانستان میں ہوئی، لیکن تعلیمی مراحل ہندوستان میں طے ہوئے اور یہاں کے باکمال اساتذہ سے استفادہ کیا، زندگی کا بڑا حصہ حیدرآباد میں گزرا، عرصہ تک مدرسہ نظامیہ میں مدرس رہے، فقہ حنفی سے خاص مناسبت تھی اور امام ابوحنیفہ ان کے تلامذہ اور ائمہ احناف سے بے حد عقیدت تھی، لجنۃ احیاء المعارف العثمانیہ ان کی اس عقیدت کی گواہ ہے، انھوں نے قاضی ابو یوسف امام محمد اور شمس لائمہ سرخسی کی کتابیں ڈھونڈ کر جمع کیں، بڑی دیدہ ریزی کے ساتھ مختلف نسخوں کا مقابلہ کیا، جان کا ہی کے ساتھ ان کی تصحیح کی، حواشی لکھے، انڈکس بنائے اور حسن و خوبی کے ساتھ طباعت کا انتظام کیا، وہ پرانے مدرسوں کے پڑھے ہوئے تھے، لیکن نئے محققین بھی ان کا لوہا مانتے تھے، افسوس ہے کہ گزشتہ ماہ علم و تحقیق کی یہ شمع خاموش ہوگئی، اﷲ تعالیٰ ان کو اپنی رحمتوں سے سرفراز فرمائے اور ان کے بیش بہاکاموں کے جاری رکھنے کا انتظام فرمائے۔
(عبد السلام قدوائی ندوی، ستمبر ۱۹۷۵ء)

 

مزیل الأوهام فیما یتعلق بالاحکام عن المخنث والخنثی

The following research paper focuses on eunuch known in our society as khwaja sara, Hijra and khusra. It address the majors questions such as who they are? Can they be organized as men or women? In parts of our society they have their rights and duties. This paper deals with issues related to their imamat(leadership), testimony, punishment and reward, hajj (pilgremage), blood money, inheritance, funeral rituals, Azan, Wazu, bath etc.   

Influence of Different Protein Sources on Growth Performance and Carcass Characteristics of Kajli Male Lambs

Three independent studies using male Kajli lambs at post-weaning, growing and finishing stages were designed and undertaken to examine the effect of different protein sources with or without probiotics on growth performance and carcass characteristics. In the trial I, 32 male Kajli lambs at three months age were randomly divided into eight groups of four animals each in a 4×2 factorial arrangement. Four iso-caloric (70% TDN) and iso- nitrogenous (22% CP) diets were formulated with four different protein sources i.e. corn gluten meal 30% (CGM), canola meal (CM), cotton seed meal (CSM) and sunflower meal (SFM) containing either 0 or 50g of probiotics (Protexin®)/ ton of feed. In trial II and III, lambs were again randomized and given the same treatment as in trial I. However, crude protein (CP) content of diets was reduced from 22 to 18% in trial II and 18 to14% in trial III. In trial I, higher (P<0.05) dry matter (DM) and CP intake, DM, CP, neutral detergent fibre (NDF) and acid detergent fibre (ADF) digestibility, blood glucose, N-balance and total weight gain were observed in lambs fed CM diet than those fed CGM, CSM and SFM diets. However, blood urea nitrogen (BUN), creatinine and serum minerals remained unaltered (P>0.05) in lambs fed diets containing different protein sources. Neutral detergent fibre and ADF digestibility and blood glucose was higher (P<0.05) in lambs fed diets containing probiotics. Dry matter and CP intake and their digestibility, BUN, creatinine, serum minerals, N-balance and total weight gain remained unaltered (P>0.05). In trial II, higher (P<0.05) DM and CP intake, blood glucose, N-balance and total weight gain were noticed in lambs fed CM diet than those fed CGM, CSM and SFM diets. However, diets containing different protein source didn’t affect (P>0.05) the DM, CP, NDF and ADF digestibility, BUN, creatinine and serum minerals. Neutral detergent fibre and ADF digestibility and total weight gain was higher (P<0.05) in lambs fed diets containing probiotics. However, diets containing probiotics didn’t influence (P>0.05) DM and CP intake, DM and CP digestibility, BUN, blood glucose, creatinine, serum minerals and N-balance. In trial III, higher (P<0.05) DM and CP intake, N-balance and total weight gain were observed in lambs fed CM diet than those fed CGM, CSM and SFM diets. However, DM, CP, NDF and ADF digestibility, BUN, blood glucose, creatinine and serum minerals remained unaltered (P>0.05) in lambs fed diets containing different protein source. Acid detergent fibre digestibility and total weight gain was higher (P<0.05) in lambs fed diets containing probiotics however, DM and CP intake, DM, CP and NDF digestibility, BUN, blood glucose, creatinine, serum minerals and N- balance remained unaffected (P>0.05). Hot carcass weights of lambs differed significantly (P<0.05) in lambs fed diets containing different protein sources with or without probiotics. However, dressing percentage (DP) and skin, feet, heart, liver and lungs weight remained unaltered (P>0.05) across all the treatments. The higher DP was observed in lambs fed CM diets, whereas it was lower in lambs fed SFM diet. Leg, loin, shoulder, breast and neck weights of the half carcass of the lambs remained unaffected (P>0.05) across all the treatments. Crude protein content of carcass was different (P<0.05) in lambs fed diets containing different protein source with or without probiotics, while moisture, ether extract and ash contents remained unaltered (P>0.05) across all treatments. The ratio of lean, fat and bone in primal cuts of half carcass (neck, shoulder, breast, loin and leg) remained unchanged (P>0.05) across all the treatments, except for the proportion of lean in shoulder, bone in loin and fat in legs, which was affected (P<0.05) by the diets containing different protein sources. In short, the lambs fed CM diets performed better than those fed CGM, CSM and SFM diets in all the three trials. Diets containing probiotics improved weight gain in trial II and III but not in trial I.