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Home > Comparative Peptidomic Profiling of Cationic Peptides from Solanum Lycopersicum under Saline Stress

Comparative Peptidomic Profiling of Cationic Peptides from Solanum Lycopersicum under Saline Stress

Thesis Info

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External Link

Author

Kanwal, Nazia

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9655/1/Nazia_Kanwal_Biochemistry_2016_UAF_10.06.2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725759092

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Antibiotics are frequently and universally common drugs used by humans in advocated and un-prescribed forms. Misuse of commercially available antibiotics has caused emergence of antibiotic-resistant microorganisms in addition to drug toxicity and other health complications. The global crisis of multi-drug resistance necessitates the development of novel antibiotics. Small peptides play a fundamental role in recruiting and promoting agents of innate as well as adaptive immune system. These are relied as good candidates for a new generation of antimicrobial drugs to treat multidrug-resistant microorganisms. The cationic peptides are also known as natural antibiotics and are produced constitutively as well as under the influence of biotic and abiotic stresses. The present project was designed to profile positively charged antimicrobial cationic peptides. Selected glycophytes (Solanum lycopersicum, Silibum marianum and Mentha piperita) and halophyte (Thallungiela halophila) were subjected to salt stress of NaCl at different molar concentrations. The glycophytes were treated with 100 mM salt for 14 days (SLS100-14) and 200 mM of salt for 5 (SLS200-5) and 10 days (SLS200-10) and halophyte was treated with 300 mM NaCl for 3 days (THS300-3). Small proteins and peptides of S. lycopersicum and T. halophila of less than 10 kDa were fractionated by strong cation exchange chromatography. All the obtained fractions were tested for bioactive potential against E. coli and active fractions of tomato plants (control and 100 mM salt stressed). The results showed that tomato plants exhibited salt tolerance at 100 mM of NaCl. The peptides of control and salt treated (SLS100, SLS200-5 and SLS200-10) tomato plants were fractionated on strong cation exchange column and fractions of control and treated with 100 mM NaCl showed significant antibacterial activities against E. coli. The fractions of control and salt-treated (100 mM NaCl) plant samples were run on LC-MS and obtained spectrum peak list were analyzed manually for independent data acquisition (IDA) with a software (Analyst, AB Sciex). The software converted total ionic currents (TIC) of peptide fragments and generated charge to mass ratios for each peak that gave profiles of all the peptides. The LC-MS data files were imported into Progenesis for retention time alignment, peak picking and relative quantitation of unique features. The output from Progenesis were subjected to a python script which removed likely the same features. These features were run through a second python script for filtering to identify x features that were most likely contributed to the bioactivity with their highest abundance in the bioactive region and not elsewhere. Then statistical modeling was employed to further narrow down the list of candidates. Sequence of bioactive peptides were identified by using SwePep database and the obtained peptide sequences were subjected to BLAST search by using bioactive peptide database, “AMPer” for the identification of protein sequence similarities of subject with already reported bioactive plant peptides/ proteins. The statistical modeling listed 12 and 16 bioactive peptides from SLC and SLS100 tomato samples, respectively. The bioinformatics investigation of these bioactive peptides identified antimicrobial cationic peptides of different classes which suggested that these peptides were expressed in tomato plants. Furthermore, bioactive study of crude protein extracts of the tomato seeds and leaves suggested that tomato seeds are rich source of proteins and have significant anti-thrombotic and antioxidant potential. The crude protein extracts of tomato seeds and leaves were found not only DNA friendly and non-mutagenic but also exhibited considerable antibacterial and antifungal activities. Keeping in view the findings of current research project, the Solanum lycopersicum (tomato) plant can be used as potent candidate for cationic peptides with bioactive potential to be used in therapeutics.
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شرح خواندگی اور معاشی خوشحالی

شرح خواندگی اور معاشی خوشحالی
پڑھو گے، لکھو گے بنو گے نواب
جو کھیلو گے، کُودو گے، ہو گے خراب
خواندن مصدر ہے اور اس کے معنی ومفہوم پڑھنے کے بارے میں ہے۔ اور معاشی خوشحالی سے مراد یہ ہے کہ انسان معاشی لحاظ سے خوش حال ہو، ان کا اٹھنا بیٹھنا، کھانا پینا، سفروحضر ایک معیاری قسم کا ہو، ان کے رہن سہن، چال ڈھال میں ایک پر مسرّت اور خوشحال انسان کی جھلک نمایاں ہو۔ معاشی طور پر خوشحال انسان ہی اپنے بارے، اپنی اولادکے بارے میں، اپنے خویش و اقارب کے بارے میں، اپنے احباب کے بارے میں مثبت سوچ کا حامل ہوسکتا ہے، اور اس معاشی خوشحالی کے لیے ایک انسان کا رشتہ تعلیم سے استوار ہونا انتہائی ناگزیر ہے۔
تعلیم انسان کو ایک عظیم انسان بناتی ہے، ایک صاحب شعور فرد بناتی ہے ،تعلیم سے روشنی میسر آتی ہے، علم ایک ایسا نور اور روشنی ہے جس سے جہالت کے اندھیرے دور ہوتے ہیں، انسان کے دل و دماغ عرفان وآگہی کے نور سے منور ہوتے ہیں ،علم ہی کی بدولت انسان حق و باطل اور خیر وشر میں فرق کرنا سیکھتا ہے۔ علم ہی کی بدولت انسان کی خوابیدہ صلاحیتیں بیدار ہوتی ہیں اورعلم ہی کی وجہ سے انسان کے رہن سہن اور طرزِ زندگی میں تہذیب وشائستگی پیدا ہوتی ہے۔ اس میں تعصب اور تنگ نظری کی بجائے فراخ دلی اور رواداری ، خودغرضی کی بجائے ایثار، غرور ونخوت کی بجائے عجز و انکسار ، حرص اور لالچ کی بجائے صبر و قناعت ، حسد اور نفرت کی بجائے محبت اور اخوت جیسے اوصاف پیدا ہوتے ہیں۔
تعلیم ہی کے ذریعے مرصعّ انسان ہی معاشی خوشحالی کا ضامن ہوتا ہے، اگر کسان پڑھا لکھا ہوگا تو اس کی کھیتی بھی زیادہ ہوگی ، اس کی فصل میں اضافہ...

الشيخ حافظ محمد إدريس وأسلوبه في تفسيره كشاف القرآن

Pashtun Ulama have always been in the service of Islam. The origin of Pashtun people in Pakistan is the province of Khyber Pukhtunkhwa formerly known is North West Frontier Province (NWFP). Pashtun Ulama have served Islam on different fronts; some have done Jihad against the non-Muslims, some of them have offered their services in the field of Islamic education, ie, teaching in Madrassas, while some have served Islam via their writing commentary of Qur’ān, explanation of Ḥadīth and juristic problems. This article discusses the worthy contributions of one of the great commentators of Qur’ān Shaykh Ḥāfiẓ Muḥammad Idrīs, who belonged to Mardan in the Province of Khyber Pakhtunkhwa, He lived in the 19th century. The article encompasses a detailed biography of the writer and his worthy contribution in serving Qur’ān, Islam and Muslims focusing on his commentary on Qur’ān in Pashto language named as “Kashshāf Al Qur’ān”. The method used for the research is descriptive and qualitative. The review literature showed that such an esteemed and vigorous scientist of different sciences of Qur’ān and his valuable contributions are out of the reach of scholars and libraries that may lead to an irreparable loss of the Islamic legacy. The study came up with the outcomes of his efforts in the field exegesis of Qur’ān, and the way and pattern he followed in interpreting different meanings of the revealed literature. The study would help out Islamic scholars and will enrich the domain of the Qur’ān’s research with new thoughts and viewpoints

The Effect of Premedication With Intravenous Lornoxicam on Propofol Injection Pain at Induction of General Anaesthesia in Adults: A Randomised Placebo-Controlled Trial

Background: Propofol is widely used for the induction and maintenance of general anaesthesia and offers many key attractive pharmacological qualities that make it suitable for these indications. However, pain on injection is one of its major drawbacks and can be very distressing to patients. There is a paucity of studies that have looked at the effect of lornoxicam on propofol injection pain either as a sole intervention or in combination with any other method. Primary objective: To determine whether premedication with intravenous lornoxicam had any effect on the intensity of propofol injection pain at induction of general anaesthesia in adult patients. Secondary Objectives: (I)To determine whether premedication with lornoxicam had any effect on the incidence of propofol injection pain at induction of general anaesthesia in adult patients.(II)To document any adverse events (allergic reactions, nausea, vomiting, gastritis and/or gastrointestinal bleeding, dizziness, phlebitis) that resulted from the administration of intravenous lornoxicam for the purposes of this study. Study Design: A single centre, randomized, placebo-controlled trial. Methods: 121 adult patients of American Society of Anaesthesiologists (ASA) physical status 1 and 2 were recruited from the anaesthesia clinic, day care surgery unit and the wards and randomized into the intervention and control groups following informed consent. The intervention group received 8mg of lornoxicam intravenously thirty minutes prior to induction of general anaesthesia while the control group received normal saline (inactive placebo) intravenously. Both groups then received a propofol-lignocaine admixture at induction of general anaesthesia and the pain scores during propofol injection were recorded using the critical care pain observation tool (CPOT). Results: Patients baseline characteristics were similar across both the intervention and the control groups. The lornoxicam group had a better mean pain score (0.6 ± 0.8) compared to placebo (4.5 ± 1.2), a statistically significant difference of 3.9 (95% CI, 3.57 to 4.32), t (119) = 21.1, P < .001. vi Conclusion: Premedication with lornoxicam, when combined with the fairly standard practice of a propofol-lignocaine admixture, significantly reduces the intensity of propofol injection pain at induction of general anaesthesia in adult patients.