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Home > Computational Analysis of Metabolic Pathways and Protien-Protien Interactions Ppls of Clinically Significant Pathogens, and Identification of Inhibitors of Ppls

Computational Analysis of Metabolic Pathways and Protien-Protien Interactions Ppls of Clinically Significant Pathogens, and Identification of Inhibitors of Ppls

Thesis Info

Access Option

External Link

Author

Sufian, Muhammad

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Molecular Medicine

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12760/1/Thesis%20PhD%20Sufian.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725777076

Similar


Salmonella enterica is a Gram-negative facultative anaerobic bacteria. It belongs to the family of Enterobacteriaceae. Infections caused by Salmonella species are major threat to the human and animal health. After 1-3 days of ingestion of contaminated food, the patient develops diarrhea, fever, vomiting, and abdominal cramps. The situation is exacerbated if not treated promptly. Whole genome sequencing projects of clinically significant serovars of S. enterica have opened new perspectives of medical research. Using the genomic data, novel approaches are being employed throughout the world to find new protein targets for drug designing and screening. Targeting the essential metabolic pathways of the bacteria is the approach which we have focused in our study. The protein sequence and metabolic pathway data of the core proteome of S. enterica was analyzed in comparison to that of Homo sapiens. Various computational tools (BLASTp, CD-HIT, and Shell scripting) and datasets (NCBI, DEG, and KEGG) were extensively utilized to find non-homologous and essential enzymes of the pathogen. We discovered 73 enzymes belonging to metabolic pathways found only in the bacteria but not in H. sapiens, and proposed them as potential drug targets. Later, we selected an essential outer membrane protein complex (LptD/E) of S. enterica involved in lipopolysaccharide assembly, as a target in search of an inhibitor of the PPI complex. Druggable sites at the interface of PPI were identified by PocketQuery followed by virtual screening of the ZINC database of commercially available compounds using the ZINCPharmer tool. Energy minimization and scoring of short-listed compounds was performed using SMINA. A rational screening of >10,000 compounds resulted in 3 compounds depicting favorable polar interactions and optimal conformation for binding with the LptD protein. We propose that this interaction may lead to block the LptD interaction with LptE and lipid molecules and in result may block the LPS assembly.
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تحریکِ پاکستان میں مادرِ ملت کا کردار

تحریک پاکستان میں مادر ِملت کا کردار
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز سامعین اور میرے ہم مکتب شاہینو!
آج مجھے جس موضوع پر اظہار خیال کرناہے وہ ہے:’’مادرِ ملت محترمہ فاطمہ جناح ‘‘
جنابِ صدر!
مادرملت سے مراد فاطمہ جناح ہے۔ محترمہ فاطمہ جناح بانی ٔپاکستان قائد اعظم محمد علی جناح رحمۃ اللہ علیہ کی چھوٹی بہن تھیں۔ ان کی ساری زندگی بانی ٔپاکستان اور پاکستان کے لیے وقف تھی۔ محترمہ فاطمہ جناح قائد اعظم رحمۃ اللہ علیہ کی معتمد ساتھی اور تحریک پاکستان میں ان کی معاون اور رفیق کار ہیں۔ تحریک پاکستان کے ہر موڑ پر محترمہ کی خدمات ناقابل فراموش ہیں۔ انہوں نے اپنی پوری زندگی قومی خدمات کے لیے وقف کر دی تھی۔ تحریک پاکستان کے دوران خواتین کی مختلف تنظیموں کی راہنمائی کے علاوہ عام مسلمان خواتین کے مسائل میں گہری دلچسپی لیتی رہیں۔ آپ خواتین میں بے حد مقبول تھیں وہ ہمیشہ مسلمان خواتین کو تحریک پاکستان کے لیے عملی کام کرنے پر آمادہ کرتیں۔ انہیں ان کی اہمیت کااحساس دلاتے ہوئے قومی خدمت کے لیے تیار کرتیں۔ خواتین کے محاذ پر تحریک پاکستان کے تمام امور کی نگرانی محترمہ فاطمہ جناح کے ذمہ تھی۔
قیام پاکستان کے بعد بھی آپ کی قومی خدمات کا سلسلہ جاری رہا ۔تعلیم نسواں کا مسئلہ ہو یا مہاجرین کی آباد کاری کشمیری مہا جر ین کی دستگیری ہو ،بہبود اطفال اور حفظان صحت کے مسائل آپ کی خدمات ہر شعبے میں جلی حروف میں لکھے جانے کے قابل ہیں۔محترمہ فاطمہ جناح کی قومی خدمات کی بناپر انہیں قوم نے بجا طور پر مادر ملت کا لقب دیا۔ مادر ملت نے قومی مسائل میں اپنے بھائی کی طرح اپنی صحت اور پیرانہ سالی کی بھی پرواہ نہیں کی۔ 1964ء کی تحریک...

A Phenomenological Study of the Lived Experiences of Tagumpay National High School Teachers Involved in Online Learning Action Cell Session

A qualitative phenomenological approach was used in this study to describe the lived experiences of Tagumpay National High School (TNHS) teachers on Online Learning Action Cell (LAC) session. LAC is a school-based professional development for teachers implemented by the Philippine Department of Education (DepEd). Due to teacher’s lack of participation on classroom LAC, a fully-online mode option is explored by offering TNHS teachers Online LAC session using Facebook as a Learning Management System (LMS). To capture the lived experience of teachers, an in-depth interview with a purposive sample of one TNHS teacher is done in the process. The data gathered went through “Hycner’s Explicitation Process” (1999, in Groenewald, 2004) which includes bracketing, delineating, clustering, summarizing and extracting unique themes. Validity and Credibility were accomplished through an intercoder agreement between researchers, Facebook chat records, bracketing, and member checking. Results identified three themes in relation to teacher’s experience of Online LAC session including usefulness, barriers, and preference. Findings revealed the major role of TNHS teacher’s context on how Online LAC is utilized. Recommendations include administrator and expert teacher working with classroom teachers and the inclusion of teachers’ voices as input in the program design, implementation and evaluation stages of Online LAC to better address curriculum needs and facilitate the delivery of high-quality professional development for teachers’ professional growth.

Element of Hopelessness and Meaninglessness in Absurdist Drama

Element of Hopelessness and Meaninglessness in Absurdist Drama Absurdism is a very deep philosophy which focuses on a totally distorted and nihilistic vision of life. This movement got momentum after the devastations of the Second World War when a heavy loss of precious lives threw Europe into the pits of utter dejection and disillusionment. The writers, poets and the philosophers of that era who were the representatives of their society gave vent to their pent up feelings and emotions and wrote extensively about the anguished state of their nation. This dejection which was the outcome of the turmoil created by the Second World War is specially focused on in this thesis. The aim of this research is to highlight the element of the absurd in man’s life which is inevitable. This absurdity in life gives birth to hopelessness and disillusionment. Dejection is a very natural feeling and it leaves a drastic effect on the lives of the human beings. A dejected person totally negates life and develops a negative attitude towards the world. The Absurdist playwrights have made this meaninglessness and despair the core issue of their work. This vision changed the whole scenario of life for them and we find an air of gloominess and dejection prevailing everything in their plays. Keeping in view the approach of these dramatists the researcher wants to probe deeper into the philosophy of life and prove whether the absurd situations in life which create despair and anguish be avoided by thinking rationally and facing life in a real perspective or whether a nihilistic approach like that of the Absurdists’ is the ultimate outcome. It is a very vast and complex issue and for such an issue quantitative approach cannot be followed so the researcher has adopted the qualitative approach. The research is text- based and different libraries have been consulted for collecting the material. The electronic media also helped in making it an authentic piece of writing. As this research is text-based so the researcher has tried her best to make it more descriptive in the light of the material available. Different Absurdist playwrights have been studied for conducting the research but the two eminent dramatists Samuel Beckett and Harold Pinter have been specifically focused on for analyzing the theme of Hopelessness and Meaninglessness. They belong to ‘The Theatre of the Absurd’ and the streak of absurdity evident in their plays displays a total bleak picture of life. In this research work an attempt is being made to let the readers visualize life in a more realistic perspective. The researcher wants the readers to analyze that life has to be accepted with all its colors no matter how dull or bright they are. The research concludes with a very rational approach towards life that it is a precious gift from God and we can make it worth living by a possible return to religion and by having a firm faith.