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Construction of Biological Association Network for Connected Components Analysis Using Graph Theory

Thesis Info

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External Link

Author

Kanwal, Attiya

Program

PhD

Institute

Capital University of Science & Technology

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Bioinformatics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11805/1/Attiya%20Kanwal%20Biosci%202019%20cust%20isb%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725785601

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The concept of big data has been around for years. Big data analysis approaches can play an imperative part in health research. This can be a helpful asset for researchers because it can reveal veiled acquaintance from a massive sum of data. In order to get insights of metabolic pathways at molecular level there is a need to present a unit framework model that serves as the cutting edge technology of sys tem biology. In this thesis data mining techniques were used to extract data from online databases automatically by developing a tool (PubMed Info Extractor). On inputing queries, relevant information about the association and interactions among biological entities have been found in the retrieved collection. The ap proach has been applied on the case study of T2DM. Cell boundaries of the found gene components were also identified. Only those components were selected that show highly expressed genes in the pancreatic endocrine cells and skeletal muscle cell for determining the disrupted pathway after analyzing the normal functional pathway of T2DM. Further, bioinformatics tools were used for the topological analysis of obtained patterns. From a network generated by using the association rule mining technique showing associations, their nature of relationship, seven strongly connected components have been identified. These components represent P53, HNF1Alpha, HNF1Beta, INSR, INS, IL-6 and GnRH as the regulators or initiators of seven different biological regulatory pathways. This research is an evidence for the association of T2DM with the genes that are involved in different pathways of cancer cell metabolism, growth regulation, proliferation control etc along with insulin signaling pathway, mTOR pathway, MODY pathway, glycolysis, lipid homeostasis, Age-rage signaling pathway, MAPK pathway, p53 pathway. Self inhibition of ngn3 is also acknowledged in these components. In diabetic patients, pancreatic islets in case of fasting lessen PKA and mTOR activity and induce Sox2 and Ngn3 expression and insulin production. Self inhibition of Ngn3 can there fore affect the insulin production. This research gives a unit framework model of system biology which gives better understanding of intrinsic disease mechanism. This research regarding T2DM which will facilitate the researchers to compre hend the system of T2DM disease mechanism and how to cure it with respect to personalized drugs.
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الباب الثاني: الشاعرۃ نازك الملائکۃ

الباب الثاني: الشاعرۃ نازك الملائکۃ
سنتحدث في ھذا الباب عن رائدۃ الشعر العربي الحر نازك الملائکۃ منذ ولادتھا إلی وفاتھا وما قدمت من أعمال أدبیۃ وشعریۃ وعلمیۃ، وأسلوبھا وکیف کانت بدایۃ نظمھا وما ھي أھم مؤلفاتھا وأشھر دواودینھا، وهي شاعرة عربية من دولة العراق ، عراقية الأصل ، وأشعارها معروفة بالحزن وقد خترت هذه الشخصية العظيمة مقارنةً مع بروين شاكر باكستانية الأصل ، لأن بروين شاكر أيضاً معروفة بأشعارها الحزينة فهناك تشابه بينهما في أمور كثيرة . والآن سنتعرف على نازك الملائكة.

An Extended Model Predictive-Sliding Mode Control for Three-Level AC/DC Power Converters

Abstract- In the proposed approach, an Extended Model Predictive Sliding Mode Controller (EMPSMC) was designed to control three-level AC / DC power converters for better dynamic performance and better achievement. The traditional proportional integration (PI) controller is used in the model predictive PI controller (MPPIC) technique to generate active power reference. However, this technique results in a significant overshoot/undershirt and steady-state error. Instead of PI, sliding mode control (SMC) is used to address these shortcomings. The performance of EMPSMC and MPPIC is compared and analyzed without interruption. The results show that the introduction of SMC reduces the time lag of the system and reduces overshoot. The simulation results validate the performance of the designed model.

Modeling the Behaviour of Pakistani Married Women Towards Contraceptive Use

A number of logistic regression and log-linear models were fitted to determine the factors, affecting the contraceptive use and impact of women’s reproductive autonomy on contraceptive use in relation with husband-wife education attainments in Pakistan by using the data from 2000-01 Pakistan Reproductive Health and Family Planning Survey. In exploratory multivariable logistic regression models’ analysis of a large nationwide survey of women in Pakistan, there was found that most of socio-demographic, socio- economic and cultural variables were significantly associated with use of contraception. A number of logistic regression models were fitted by adjusting different, demographic, socioeconomic, cultural and women’s reproductive autonomy variables, to examine the effect of these variables on ever and currently use of contraceptive methods by the respondents. Predicted probabilities of ever and current contraceptive use through logistic regression models were also worked out by taking the most prominent independent variables from each model, separately. Number of living children, respondents’ education attainments, husbands’ desire for more children, Sex preference for the next child and spouses’ discussion about family planning were found more prominent predictor variables for both ever and currently use of contraception in this study. Women’s reproductive autonomy variables; decision to have no iiimore children, decision to delay the next child, and decision to use the family planning methods, were also found significantly associated with use of contraception. A number of log-linear models were fitted with the help of partial and marginal association tests for the variables, which emerged significantly associated in the logistic regression modeling process in case of ever and currently use of contraceptive, to analyze the factors affecting the contraceptive use in Pakistan. Standardized values of the models’ parameters estimates were computed to see the pair wise association within all levels of the variables. Women’s reproductive autonomy in relation with husband-wife education attainments and contraceptive use were examined through log-linear modeling process and predicted probabilities of contraceptive use. The results of this studies revealed that the prevalence of contraceptive use increases with the increase in the number of living children and education attainment levels of the respondent. From standardized values of models’ parameter estimates found a significant positive association between duration of education and prevalence of contraceptive use and number of living children only when that duration had exceeded six years. It was perceived from the results that Pakistani women who lived in urban area are more likely to have used contraception than those who lived in rural area, even though they were more educated. From the results of predicted probabilities of contraceptive use, it was found that spouses’ communication about family planning and women’s education emerged important predictors of contraceptive use. Contraceptive use was found consistently associated with husband-wife discussion about family planning and their education attainments through out logistic regression and log-linear modeling process. From the predicted probabilities of contraceptive use and standardized values of hierarchical log-linear models’ parameters estimates it is ivperceived that the husband’s desire for more children and his preference for children of a given sex, particularly sons, are obstacles to the application of contraceptive measures. Women’s reproductive autonomy appeared to be strongly associated with contraceptive use and spouses’ education attainments. Although lower fertility is commonly associated with women’s reproductive autonomy, the husband’s education influences his wife’s fertility intentions more than her own education does. Our studies were shown a strong relationship between women’s education and knowledge about family planning clinic and contraceptive use. The findings of this study reaffirm the overarching importance of women’s education, which influences both directly and indirectly the implementation of birth control measures. Improvement of women’s education can also help in reducing the cultural factor of sex preference for the next child, which is a strong barrier to contraceptive use. The data also suggest the need for giving great attention in future studies to the relationship between spouses, which can solve conflicting fertility desires. To further promote contraception, a greater awareness must be achieved among women with regard to birth control. Programmes should be set up to encourage women to practice family planning measures more widely in their early reproductive lives and not to seek so strongly a male child. Despite highlighting a number of determinants of contraceptive use in Pakistan, this study also demonstrates the importance of methodological issues in the contraceptive prevalence studies. It is suggested that the predicted probabilities must be computed in the prevalence of contraceptive studies, as these probabilities precisely provide the degree of assessment of the acceptability of contraception and log-linear model analysis technique also recommended for the future studies because it enhances confidence in the results and vprovides a specific measure of associations within all variables under study in multi-way contingency table for large scale data set.