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Cytochrome P450 Genetic Variants As Predictor of Personalized Therapy in Pakistani Population

Thesis Info

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Author

Riaz, Sana

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2020

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/14434/1/Sana%20Riaz_Biochem_2020_QAU_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725808931

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The CYP2C9 and CYP2C19 polymorphism are associated with pretreatment drug response prediction, metabolism and disposition. Pakistan consists of a population comprising of various ethnic groups residing in different regions of the country each claiming diverse ethnic origins. The identification of the CYP450 genotypic composition of these populations is therefore necessary to avoid adverse drug reactions in these individuals. The study objective was to investigate the CYP2C9*2, CYP2C9*3, CYP2C19*2, CYP2C19*17 frequency in nine Pakistani ethnic groups and to check their association with hypertension. DNA was extracted and analyzed by AS-PCR. Four phenotypes were observed and they were: extensive metabolizer (EM), poor metabolizer (PM), intermediate metabolizer (IM) and ultra-rapid metabolizer (UM). Regarding population, over all the percentage of predicted PM allele (CYP2C19*2) was 29.0% compared to (CYP2C19*17) UM allele 23.70%. Among the studied groups, Saraiki and Brahui showed highest percentage of PM allele (40%, 36%) whereas Parsi and Hazara had highest percentage of UM allele (37% and 30% respectively). In case of CYP2C9, CYP2C9*2 showed highest frequency in Saraiki (51%) and Burusho (45%), while in case of CYP2C9*3 Brahui (49%) and Hazara (47%) showed highest frequency. Overall, 75%, 25%; 64.2%, 35.8 % of CYP2C19*2 and 66.6%, 33.4%; 75.6%, 24.4% of CYP2C19*17 wild type and mutant allele frequency was observed in patients and controls (p<0.05). Similarly, 51%, 49%; 76%, 24% of CYP2C9*2 and 46.5%, 53.5%; 46%, 54% of CYP2C9*3 wild type and mutant allele frequency was observed in patients and controls (p<0.05). In conclusion the high prevalance of CYP2C9*2, CYP2C9*3, CYP2C19*2 and *17 in Pakistani population leads to the recommendation of a pre-treatment test to monitor drug response and dosage (personalized medicine) to avoid post-treatment adverse drug reactions.
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راجیو گاندھی

راجیو گاندھی
وسط مدتی انتخابات کے دوران میں مدراس کے ایک انتخابی جلسہ میں تقریر کے پروگرام سے کچھ دیر پہلے ہی ہندوستان کے سابق وزیراعظم اور انڈین نیشنل کانگریس کے صدر جناب راجیوگاندھی ایک بم دھماکے میں لُقمۂ اجل ہوگئے۔
اس وسط مدتی انتخاب کے بارے میں جہاندیدہ دانشورانِ ملک اور خودمقتول ...راجیوگاندھی یہ خدشہ ورائے ظاہر کررہے تھے کہ اس وسط مدتی انتخاب میں زبردست تشدّد کاامکان نظرآرہا ہے ۔یہ کس کومعلوم تھاکہ جس تشدّدکے امکان کااظہار کیاجارہاہے وہ ملک کی اس عظیم ہستی ہی کو اپنی منحوس لپیٹ میں لے لے گا۔مگرانہونی ہوکررہی اورملک اایک ایسے رہنما سے محروم ہوگیا جو دورِ حاضر کاہیرو تھا اور مستقبل کی روشن قندیل، اور جس سے ملک و کوبڑی بڑی امیدیں وابستہ تھیں۔
جناب راجیو گاندھی کے حادثۂ قتل میں کس پارٹی، کس گروہ ،کس ملک یا کس فرد کاہاتھ ہے اس کے بارے میں کچھ کہنا قبل از وقت ہے۔ابھی تک کسی نے اس کی ذمہ داری قبول نہیں کی ہے۔عام طور پرایل ۔ٹی۔ٹی دہشت پسندوں پرشک ظاہر کیا جارہا ہے۔اوراس کے لیے اخبارات کی اطلاع کے مطابق کچھ ٹھوس ثبوت بھی جائے واردات سے مِلے ہیں۔ جو عورت اپنے جسم پر بم باندھ و لپیٹ کر خودبھی ہلاک ہوئی ہے اور جناب راجیوگاندھی کے ساتھ اوردوسرے تیرہ افراد کوہلاک کرنے کاباعث بنی ہے اس کے متعلق بھی عام قیاس یہ ہی ہے کہ وہ ایل ٹی ٹی گروہ سے تعلق رکھتی ہے۔مگرابھی یہ سب قیاس آرائیاں ہی ہیں،یقین و قطعیت سے کچھ نہیں کہا جاسکتا ہے۔حکومتِ ہند نے تمام حادثۂ قتل کی تحقیقات کے لیے بروقت ایک کمیشن بنادیا ہے جوعرصہ تین ماہ میں اس سلسلے میں اپنی رپورٹ پیش کرے گا۔ اس سے پہلے جوبھی اس سلسلے میں اظہار ِ خیال کرے گا وہ صرف قیاسات ہی کے زمرے میں ہوگا۔اورحقیقت ویقین کاگمان کرنا...

Intoxicant Use in Undergraduate Medical Students of Islamabad, Pakistan

Intoxicant use and subsequent substance abuse is the leading health problem in younger age groups in contemporary times, and across the globe. It contributes to morbidity, mortality and economic loss. It also disturbs the social system of a community and creates a serious threat to global health. The aim of this study was to determine the use of intoxicants among medical college students of Islamabad and to identify the associated factors with intoxicant use. A cross-sectional research design was used to gather survey data from two private medical colleges. Data from a final sample of 310 respondents was analyzed using descriptive statistics. It was found that smoking was the most prevalent intoxicant used amongst the participants (21.9%). The other most common prevalent use of intoxicants included alcohol (6.1%), Naswar (powdered tobacco dip) (6.8%) and Heroin (3.9%). The male gender and need for mental relaxation were found to be statistically significant with cigarette smoking. Most of the students indicated that they did not feel guilty about using intoxicants. It is important that medical college administration develops policies to control intoxicant use among students, including and not limited to awareness campaigns, counseling and support for stress relief.

Stochastic Models for Population of Pakistan

TITLE: Stochastic Models for Population of Pakistan PAGES: 182 STUDENT: Muhammad Zakria SUPERVISOR: Professor Dr. Faqir Muhammad UNIVERSITY: Allama Iqbal Open University, Islamabad, Pakistan YEAR: 2005-2009 SUBJECT: Statistics DEGREE: Ph.D Population of Pakistan is projected by scientists, bureaus and countries using different methodologies. In this study, population projections, its age-sex distribution vision 2030 and inequality of the recorded and projected age-sex distribution is projected by different methods. Moreover, the reproductive cohort measure and fertility trends of the population during the last 20 years are measured. The said goals are achieved by using the population censuses data. First of all, the quality of all censuses data is checked and found to be very poor especially of 1972 census. Different popular smoothing techniques are used to smooth the census data and strong smoothed data is used for further analysis. A time series model i.e. ARIMA (1, 2, 0) W was found to be a parsimonious model and population is projected for the next 20 years. It would be approximately 230.68 million in 2027 along with 95% confidence limits 193.33 million and 275.25 million. The age sex distribution as well as iv the total population is also projected by using the Modified Markov chain method for 40 years ahead since 1981. The Projections by the Time series models and the Modified Markov chain method are more close to the projections of four internationally known bureaus i.e. (WPP 2008; People Facts and Figures & Total Population by Country 2009) and greater than (NIPS 2006; IDB 2008). Gini coefficients of the projected age sex distribution indicated the medium level of concentration during the next 20 years. Approximately 43.74%, 47.27% and 45.46% decrease in TFR has been seen in rural areas, urban areas and in Pakistan respectively during 1984-2005. Different polynomial models are studied and third degree polynomial model is recommended to fit on the age specific fertility rates of Pakistan and its rural urban regions.