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Cytogenetic and Molecular Analysis of Chronic Myeloid Leukemia at Hyderabad Sindh.

Thesis Info

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Author

Ujjan, Ikram Din

Program

PhD

Institute

Isra University

City

Hyderabad

Province

Sindh

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2042/1/16120s.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725809381

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BACKGROUND: Leukemia is defined as a neoplastic proliferation of hematopoietic tissue of white blood cell precursors in bone marrow CML accounts for 20% of all leukemias in adult patients. One of the characteristic findings in CML subjects is the presence of the Philadelphia (Ph) chromosome and Bcr-Abl oncogene. Philadelphia (Ph) is the shortened chromosome 22, formed by reciprocal translocation between the long arms of chromosomes 9 and 22, t (9; 22). OBJECTIVES OF STUDY: — To determine the frequency of Philadelphia chromosome in CML. — To evaluate the standard and variant translocation in CML. — To determine frequency of other chromosomal abnormalities in Philadelphia +ve CML. — To detect bcr-abl gene rearrangement and to determine the frequency of the b2a2 and b3a2 transcripts in Philadelphia + CML by PCR. SUBJECTS AND METHODS: The study was conducted at the Department of Pathology, Liaquat University of Medical and Health Sciences, Jamshoro and Isra University Hospital, Hyderabad during May-to-September 2014. Bone marrow and peripheral blood samples from a total of 145 diagnosed cases of CML were collected. Cytogenetic analyses were performed using karyotyping as per the international system for human cytogenetic nomenclature guidelines. All karyotpic images were analyzed using the Cytovision software. In order to identify BCR-ABL transcripts, RT-PCR was performed. Statistical analysis of the data was done using SPSS-version-21.0. Results: Of the 145 samples, a total of 133 (91.7%) were positive for the Ph (Ph+) while 12 (8.2%) were negative for the Ph (Ph-). Of the 133 Ph+ samples, standard chromosome was noted in 121 (90.9%), simple variant in 9 (6.7%) and complex variants in 3 (2.2%) of the samples. All the Ph+ samples (n=133) showed BCR-ABL positivity. Of the 12 Ph- samples, a total of 7 (58.3%) were BCR-ABL-positive and 5 (41.6%) were BCR-ABL-negative. Various types of molecular abnormalities were noted in in responders and in non responders as shown in table below. Out of 11 patients, who responded on Glivec, 8 patients showed b3a2 transcripts abnormality while 3 cases had b2a3 abnormality. Both non-responders showed b2a2 + e 19a2 transcripts. CONCLUSION: Philadelphia frequency of 90.9%. Out of 133 Ph +ve all were bcr-abl +ve while out of 12 Ph-ve 7 were bcr-abl +ve while 5 were –ve. Cytogenetic and molecular analysis has become mandatory in order to make a correct diagnosis and predict/monitor response to newer molecular targeted treatment modalities. KEY WORDS: Philadelphia chromosome, BCR-ABL, Chronic Myeloid Leukemia, cytogenetic analyses, Sindhi population.
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طبقاتی نظام میں ہوا کی تقسیم

طبقاتی نظام میں ہوا کی تقسیم

محمدجمیل اختر

درخت کم تھے، آبادی زیادہ اور ہوا اِس قدر آلودہ تھی کہ لوگ سانس لینے کی خاطر آکسیجن سلینڈر اپنے ساتھ رکھتے۔۔۔جگہ جگہ آکسیجن اسٹیشن بن گئے تھے جہاں لوگ لمبی قطاروں میں اپنی اپنی باری کا انتظار کرتے رہتے۔۔۔بڑی بڑی کمپنیاں دن رات اپنے اشتہارات تقسیم کرتی رہتیں کہ اگر اپنے پھیپھڑوں کو تندرست وتوانا رکھنا چاہتے ہیں تو اُن کی کمپنی کا آکسیجن سلینڈرحاصل کریں،اگرچہ فضا میں آکسیجن اب بھی موجود تھی لیکن اِن کمپنیوں نے جدید تحقیق سے یہ ثابت کر دیا تھا کہ اب بغیر آکسیجن ماسک کے سانس لینا زندگی کے لیے خطرہ ہے سو لوگ سانس لیتے ہوئے گھبرانے لگے۔

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