Search or add a thesis

Advanced Search (Beta)
Home > Detection and Sequence Determination of Begomoviruses Associated With Leaf Curl Disease of Capsicum Spp.

Detection and Sequence Determination of Begomoviruses Associated With Leaf Curl Disease of Capsicum Spp.

Thesis Info

Access Option

External Link

Author

Yasmin, Samina

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9064/1/Samina_Yasmin_Botany_HSR_2018_PMAS_06.04.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725832728

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The genus begomovirusbelongs to the family Geminiviridae that have emerged as serious constraints worldwide and infect variety of crops including vegetables, ornamental plants and weeds. To determine the diversity of begomoviruses on chillies,samples were collected on the basis of symptoms from Attock, Talagang, Chakwal, Islamabad and Hyderabad. Samples were tested by TAS-ELISA against begomoviruses by use of monoclonal antibodies of African cassava mosaic virus (ACMV), Indiancassava mosaic virus (ICMV) and Okra leaf curl virus (OLCV). The results indicated that all isolates showed range of high to moderate or low level of reaction. A panel of four monoclonal antibodies of ACMV, three of ICMV and three of OLCV were used. Epitope profile pattern slightly differed within each group, depending upon the location and concentration of the virus in the tested isolates. It was also confirmed that symptomatic chilli samples contained multiple infections of the begomoviruses in the studied area. Diverse patterns were found indicating great diversity among begomoviruses infecting chillies. The core region was amplified using two sets of degenerate primers. The sequencing data of nineteen samples indicates the presence of nine different species of begomoviruses, namely;Chilli leaf curl virus (ChLCV),Mesta yellow vein mosaic virus (MYVMV), Tomato enation leaf curl virus (ToELCV), Tomato leaf curl Karnataka virus (ToLCKV),Tomato leaf curl Gujarat virus (ToLCGV),Papaya leaf curl virus (PLCV),Tomato yellow leaf curl virus (ToYLCV),Chilli leaf curl Oman virus (ChLCOV),Pedilanthus leaf curl virus(PeLCV).More than one virus was isolated from the single sample indicating 1 12 thepresence of mixed populations of begomoviruses in chilli crop. Full length genome of four different begomovirus species was obtained, (248)Pedilanthus leaf curl virus (PeLCV), (249)Cotton leaf curl Burewala virus (CLCuBV), Pepper leaf curlvirus (PepLCV) and (251)Tomato leaf curl Gujarat virus (ToLCGV). No evidence for the presence of DNA-B was available, using abutting primers from the Intergenic Region (IR), when the same samples were tested. This indicates the monopartite nature of begomoviruses isolates, associated with betasatellites and alphasatellites. It becomes clear that CLCuBV and PepLCVwere associated with PLCVβ and CLCuBα. However PeLCV possesses PLCVβ but there was no association of alphasatellites. Furthermore, ToLCGV was associated EYVβ; however, alphasatellite was absent. Apart from understanding the nature of mix infection, this study has both epidemiological and pathological implications. Diseases caused by begomoviruses and associated DNA satellites have been expanding rapidly both in geographical distribution and host range. Given the presence of large number of begomoviruses throughout Asia and Africa, and ability of DNA-β to substitute for DNA-B, the probability may exist that new species/strains of begomoviruses emerge. Any effort towards developing resistance to disease, either by conventional or nonconventional means would be wise to take into account the possibility of more complex situation becoming important in chilies in the future.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

کتاب " قدرتی وسائل اور ان کا استعمال" (تعارف و تبصرہ)

کتاب " قدرتی وسائل اور ان کا استعمال" (تعارف و تبصرہ)

تبصرہ نگار:ڈاکٹر ساجد اقبال

(ایسوسی ایٹ پروفیسر۔ گورنمنٹ ڈگری کالج، بھلوال ،سرگودھا-پاکستان)

نام کتاب

قدرتی وسائل اور ان کا استعمال: اسلامی اور سائنسی علوم کے تناظر میں

مصنف

ڈاکٹرعبد المنان چیمہ

ناشر

ایشین ریسرچ انڈکس، اسلام آباد(2023)

آئی ایس بی این

9786277680060

بسم اللہ الرحمٰن الرحیم

 ڈاکٹر عبدالمنا ن چیمہ شہر ِ اقبال سیالکوٹ میں پیدا ہوئے ۔انتہائی محنت اور لگن سے اپنی ابتدائی تعلیم شہر اقبال سیالکوٹ میں مکمل کرنے کے بعد عملی زندگی کا سفر شروع کیا ۔بعدازاں اعلیٰ تعلیم کے شوق اور حصول کے لئے برادر عبدالمنان چیمہ نے یونیورسٹی آف سرگودھا کا انتخاب کیا۔2010ء میں ایم فل میں داخلہ کے لئےشاہینوں کے شہر سرگودھا کا رخ کیا۔ایم فل کے بعد 2014ء میں جامعہ سرگودھا میں پی ایچ ڈی میں داخلہ لیا۔ ڈاکٹر عبدالمنان چیمہ نے 2022ء میں "اسلام میں قدرتی وسائل و ذرائع کا تحفط اور استعمال کے اصول و آداب" کے عنوان پر میری نگرانی میں ایک انتہائی خوبصورت اور جاندار لکھ کر ڈاکٹر یٹ کی ڈگری حاصل کی ۔یہ مقالہ اپنے موضوع اورمواد کے حوالے سے انتہائی اہمیت اور انفرادیت کا حامل ہے۔انسانی زندگی کےلئے حیوانات ،نباتات اور معدنیات جیسوں وسائل کی مثلث کا پایا جانا انتہائی ناگزیر ہے۔ان کا استعمالات میں اسراف نہیں ہونا چاہئے ۔(وَّكُلُوْا وَاشْرَبُوْا وَلَا تُسْرِفُوْا-)،اللہ تعالیٰ نے تمام جانداروں کو پانی سے پیدا کیا۔( وَجَعَلْنَا مِنَ الْمَاءِ كُلَّ شَيْءٍ حَيٍّ۔القرآن)

یہ بات میرے لئے انتہائی مسرت اور خوشی کا باعث ہے کہ ڈاکٹر چیمہ نے اپنے مقالہ کو مزید بہتر بناتے ہوئے افادہ عام کے لئے کتابی شکل میں شائع...

Comparative Effect Of Tetrapack Juices And Fresh Fruit Juices On Blood Glucose Level Among Healthy Individuals Comparative Effect of Juices on Blood Glucose Level

Uses of tetra pack juices enhances the risk of diabetes and obesity. People were unaware of impact of fresh fruit juices on health. Objective: To compare the effect of tetra pack juices and fresh fruit juices on blood glucoselevels among healthy individuals Methods: 10 participants were selected using an internationalstandard Glycemic Index (GI) test protocol. After getting their fasting blood sample theywereinstructed to consume all the juice served in a period of 5 min. Further blood samples were takenat different intervals of time that is 0, 30, 60, 90, 120 and 150 minutes after consumption. Participants wereremained sedentaryduring each session. Blood was obtained by finger-prick and tested by the glucometer. Results: The glycemic index of Apple juice Nestle has the glycemic index of 95.87, Orangejuice Nestle has the GI value 93.78, Apple juice fresh has the GI value of 92.17 and Orangejuice fresh has the glycemic index value of 99.07, respectively Conclusions: Detailed study of glycemic index of tetrapack juices (Nestle Apple and Orangejuice) and fresh fruit juices (Apple and Orange juice) showed similar impact on the blood glucose level of healthy individual. In the study, both types of juices were found to be equally hyperglycemic (GI 70+) causing a fast rise in blood-sugarlevels, hence should not be given to diabetic patients

Expression of Micro-Rnas and Brcai and Brca2 in Breast Cancer Patients and Their Association With the Grade and Stage of Tumor

Breast cancer (BC) is the most frequently reported and diagnosed cancer worldwide and is one of the leading causes of mortality in women. Pakistan has the highest breast cancer incidence rate in Asia and majority of the patients present with advanced stage disease with poor survival. This situation accentuates towards the need of a safe and reliable method for early diagnosis of BC to improve the survival rate. MicroRNAs (miRNAs) are small non-coding RNAs. Abnormal expression of miRNA is a sign of various diseases, including cancer. They are remarkably stable in serum and other body fluids and can serve as reliable marker for early diagnosis of BC. The present study has included miRNA 497 and 195 which have not been studied in detail and correlated with clinico-pathological parameters in breast cancer till date. Hereditary breast cancer occurs in 5–10% of women, but those carrying BRCA1 and BRCA2 gene mutations have 40 to 84% life time high risk. Therefore, evaluation of BRCA genes mutations is helpful in predicting BC development on contralateral side and in siblings. The objectives of the present study were to assess the level of expression of miRNAs 497 and 195 in BC patients and to detect mutations in BRCA1 and BRCA 2 genes in BC patients. Additionally, to determine the association of grade and stage of BC with expression status of miRNAs. The samples and relative information of the patients were collected from various hospitals of Hyderabad and Karachi. A total of 209 females including 139 diagnosed BC cases and 70 controls matched for age were studied. Peripheral blood samples were used for extraction of total RNA. cDNA for miR-497 and miR-195 were prepared by using miR-497 antisense primer /Oligo(dT)18 Primer and miR-195 stem loop RT primers / gene specificantisense primer. GAPDH and U6 IT were used as control. The levels of expression of miRNA were recorded through amplification by qPCR. For detection of any mutation in BRCA 1 & 2 genes, DNA bands were identified by gel electrophoresis and visualized under Ultravioletilluminator. The ages of patients ranged from 25 to 65 years and majority of them belonged to 5th decade of life (71.22%). Only 15 % of the patients had history of BC in first degree relative. All of these patients (100%) presented with unilateral breast lump and out of these, 91.3% had breast lump larger than 5.0 cm. Axillary lymph node involvement was present in 64% of these patients. More than half of the patients (66.1%) were in grade II followed by 19.4% in grade I differentiation. The majority of BC patients (55.39 %) were in stage III. In total, out of 139 cases, 76.97% of BC patients and 8.57% of the controls showed downregulation of miRNA 497, while 72.66% of the BC patients and 15.71% of the controls showed downregulation of miRNA 195. Out of 89 patients who were axillary lymph node positive, 76.4% and 71.91% showed downregulation of miRNA 497 and 195 respectively. In contrast, out of the rest of 50 cases, who were axillary lymph nodes negative, 78% and 74%showed downregulation of miRNA 497 and 195 respectively. The results were highly significant at p < 0.001 in both groups. Results for correlation of both miRNAs with histological grade and clinical stage revealed that out of 27 cases of grade I, 92 cases of grade II and 20 cases of grade III BC, 55.55% (p=0.01), 85.86% (p=0.0001) and 65% (p=0.001) showed downregulation of miRNA 497 respectively, while 62.96% (p=0.001), 75% (p=0.0001) and 75% (p=0.001) revealed downregulation of miRNA 195 respectively. Almost similar results were found when correlation of miRNA 497 and 195 were done with clinical staging. It showed that, out of 21 cases of stage I, 39 cases of stage II, 77 cases of stage III and 2 cases of stage IV BC, 85.71%(p=0.01), 79.48%(p=0.001), 72.72%(p=0.001) and 100%(p=0.00001) of the cases showed downregulation of miRNA 497 respectively, whereas 80.95%(p=0.001), 64.1%(p=0.002), 74%(p=0.0001) and 100%(p=0.00001) of the cases showed downregulation of miRNA 195 in the above mentioned stages, respectively. The miRNAs 497 and 195 have been reported to be downregulated in BC. Few studies have been done regarding the level of expression of these miRNAs and their correlation with axillary lymph node involvement, grade and stage of BC. The results of the present study are highly significant and showed that levels of expression of both of these miRNAs are markedly downregulated in BC patients irrespective of lymph node involvement and grades and stages of tumor. All of these BC cases were also evaluated for selected mutations in BRCA 1 and BRCA 2 genes and the results showed the appearance of bands for wild-type alleles in all cases of BC. None of the case showed presence of bands for mutant alleles in any of the three selected founder mutations. In conclusion, the present study reports a significant association of miR-497- and miR-195 with histological grading, lymph node involvement and clinical staging. Both of these miRNAs are significantly down regulated in all grades and stages of BC, even in lymph node negative patients hence, they may be used asreliable, non-invasive biomarkers for the diagnosis and early detection of BC.