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Home > Determination and Reduction of Aflatoxins by Gamma Irradiation in Wheat, Maize and Rice

Determination and Reduction of Aflatoxins by Gamma Irradiation in Wheat, Maize and Rice

Thesis Info

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External Link

Author

Sabahat

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2009

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1606

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725838414

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Cereals grown world-wide are used as staple food. Due to high temperature, humidity and non-scientific storage prevail the growth of certain species belong to family Aspergillus. Mycotoxins are small (MW ~700), toxic chemical products formed as secondary metabolites by fungal species that readily settle on crops and infect them with toxins in the field or after harvest, during transport and storage. These compounds cause a potential threat to human and animal health through the intake of food products prepared from these commodities. Generally, crops that are stored for more than a few days become a potential target for mould growth and mycotoxin formation. Mycotoxins can occur both in temperate and tropical regions of the world, depending on the species of fungi. The extraction, clean up and analysis methodology for aflatoxins in cereals (maize, rice and wheat) were validated and found it efficient and good in respect of recovery, reproducibility, repeatability, resolution and cost-effective. The extraction solvent (acetonitrile and water) gave ≥ 85% recovery in spiked cereal samples using MycoSep-226 column. The sensitivity (LOD) of HPLC- FLD was higher as compared to HPLC-UV-Vis after derivatization of sample extract with trifluoroacetic acid (TFA). The collected cereals samples from Faisalabad Division, Pakistan exhibited high level of aflatoxins but after treatment with γ-irradiation, more than 95% reduction in AFB 1 and ≥ 97% in Total (∑AFB 1 , AFB 2 , AFG 1 and AFG 2 ) were observed at a dose of 6 kGy. Significant affect of radiation was found in wheat samples because substantial reduction (≥ 95%) was found in AFB 1 and total aflatoxins at high dose of radiation. The level of aflatoxin contamination was far behind that the recommended limits of EU and FDA, USA. From the results it is evident that γ- irradiation is a good technology to remove contaminants like aflatoxins from cereal commodities. The food quality of irradiated cereals was evaluated to study any chemical change in protein (amino acids), fatty acid composition and fungal biomass using different methods. The total biomass (CFU/g) showed linearity behaviour as increasing the dose level of gamma irradiation. In some samples of cereals, no microflora (fungi) was observed after 72 hrs. of incubation. The fatty acidcomposition revealed that linolenic acid and oleic acid showed decrease whereas no significant change was found in other fatty acids. The unsaturated and poly unsaturated fatty acids remained same after γ-irradiation exposure. The amino acids profile showed that leucine, lysine, tryptophane and valine increased while methionine, phenylalanine and threonine decreased in composition as increased the dose level of exposure of gamma irradiation. Overall no major difference was observed in any essential amino acids. The permanent use of contaminated cereals in the area may pose certain diseases like hepatitis, cancer of liver or effect of other major organs of the human body.
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فصل پنجم: آیاتِ استفہام میں فہمِ عقیدہ آخرت (تفسیر تدبرِ قرآن کی روشنی میں تخصیصی مطالعہ)

فصل پنجم: آیاتِ استفہام میں فہمِ عقیدہ آخرت (تفسیر تدبرِ قرآن کی روشنی میں تخصیصی مطالعہ)

حیات بعد الممات کا سوال ایک فلسفیانہ سوال نہیں ہے بلکہ اس کا ہمارے عملی زندگی سے بہت گہرا تعلق ہے اگر انسان کو جوابدہ کا احساس نہ ہو تو وہ اپنے اعمال پر کبھی بھی محاسبہ کرنا پسند نہیں کرے گا اس کے اخلاقی تصورات ہی بدل جائیں گے اس کا پورا اخلاقی نظام خود غرضی، بے حسی اور نفسانیت کی بنیاد پر تعمیر ہوگا اس کے برعکس اگر اعمال کی جوابدہی کا تصور ہوگا تو وہ اچھے یا برے انجام کے باعث نتیجے پر غور کرے گا۔جو لوگ قیامت کا انکار کرتے ہیں انکے بارے میں قرآن میں بیان کیا جا رہا ہے:

"وَقَالُوْٓا ءَ اِذَا كُنَّا عِظَامًا وَّرُفَاتًا ءَاِنَّا لَمَبْعُوْثُوْنَ خَلْقًا جَدِيْدًا"۔[[1]]

"اور انہوں نے کہا کہ جب ہم زمین میں گم ہو جائیں گے تو کیا ہم پھر نئے سرے سے پیدا ہونگے؟"

اس آیت میں منکرین قیامت کی روش بیان کی جا رہی ہے کہ وہ لوگ بطور تعجب کے کہتے ہیں کہ ہم مر جائیں گے ہڈیاں بوسیدہ ہو جائیں گی ہمارا وجود مٹی میں مل جائے گا تو دوبارہ کیسے پیدا ہو سکتے ہیں ؟ جبکہ یہ بات واضح ہے کہ جو خالق انکو بغیر کسی ماڈل کے پہلی بار تخلیق کرنے پر قادر ہے تو دوبارہ پیدا کیوں نہیں کر سکتا بلکہ اس کے لئے تو دوبارہ پیدا کرنا بہت ہی آسان بات ہے ۔

"اسلام نے وضاحت فرما دی ہے کہ کارخانہ ہستی کا حدوداربعہ صرف وہی عالم رنگ و بو نہیں ہے جو تمہارے چاروں طرف پھیلا ہوا ہے میدانوں ، کھلیانوں، دریاؤں ، پہاڑوں ،آبشاروں، بادلوں ، آسمان...

ظہرین کے اوقات میں اشتراکات: اسلام کے پانچ بڑے فقہی مکاتب فکر کے تناظر میں

In India scholar‘s had played incredible role for ‘Hadith’. The service of Indian Muslim scholars about methodology of Hadith is much more than other countries scholars in the last 50 years of thirteenth century. Their name should be written in golden words. There services about the methodology of Hadith are great one. Muslim scholars of sub-continent had taken keen interest in the research work of Hadith and its explanation. If we evaluate the theological history of India that ; Shah Waiullah ‘and his family worked day and night for expansion and publishing of’ Hadith  ‘ Thirteenth century of hire is called a century of revolution as concerned for Hadith. Because in that era proper theological institutions were set up and very strong institutions had spread knowledge and study of methodology of Hadith

Socio-Cultural Factors Affecting Anemia and its Effects on Mother, Child Health in the Rural Areas of District Faisalabad, Punjab, Pakistan

Anemia a common blood disorder occurs when the level of healthy red blood cells (RBCs) in the body becomes too low. This can lead to health complications because red blood cells contain hemoglobin, which pass through oxygen to the body’s tissues. Anemia causes a variety of complications, including fatigue and stress on bodily organs. Anemia status can be affected by nutritional deficiencies, infectious diseases, genetic disorders, reproductive complications, and poverty. Anemia affects approximately 2 billion people at worldwide but disproportionately affects women and children. Women of reproductive age (15-49) in Pakistan are 68 million and 50 percent of them are anemic. Children under 5 years are 18 % of population and 45-60 percent are anemic due to poor diet. This study has identified factors of anemia, and its effect on mother and child health. The main objective was to investigate mother and child anemia conditions within socio- cultural, demographic and nutritional context and effect of anemia on mother child health. Cross-Sectional study was conducted in rural areas of District Faisalabad, Punjab, Pakistan. Both quantitative and qualitative methods (Triangulation) were used to get meaningful detailed information. For capturing maximum variation on socio-cultural, demographic and nutritional aspects, the study was conducted in all four rural towns of the district Faisalabad. This research design enhances the scope of study regarding generalization of research findings. A random sample of 400 (25 women from each village) mothers aged 18-49 with at least one child (under five year) was taken to explore the research objectives through pre-designed interviewing schedule with open and closed ended questions. In qualitative study detailed information through 8 focus group discussion of 4-12 women were collected. A blood sample was obtained from mothers and their last child to determine hemoglobin levels. Univariate, bivariate and multivariate analysis demonstrate that education, family type, income, attitudes towards health facilities, violence, smoking, pre and postnatal care, blood loss during abortion, pica and calories intake were found to be associated with symptoms of anemia and level of hemoglobin of mother and child. The prevalence of anemia was found 33.3 (hemoglobin >9.0g/dl), 42.3 (hemoglobin 9.0-11.0 g/dl) and 11.1 (hemoglobin <11.1 g/dl) percent of the respondent had mild/normal, moderate and severe whereas 20.0 (hemoglobin >8.5g/dl), 41.8 (hemoglobin 8.6-10.0 g/dl) and 38.8 (hemoglobin <10.1g/dl) percent of children had mild, moderate, and severe anemia. With the increase of education of mother, family income symptoms of anemia decreased and level of hemoglobin increased both for the mother and child. Gender preferences, attitudes towards health facilities, smoking and violence were also found significant. Among these variables effect of violence on reproductive health caused a severe increase in symptoms of anemia and with decreasing level of hemoglobin. Age at marriage, pre- postnatal care was negatively associated with symptoms of anemia and positively associated with level of hemoglobin. Whereas blood loss and communicable variables were positively associated with symptoms of anemia and negatively associated with level of hemoglobin in both of mother and child. Consumption of calories intake per day and pica habits were marginally associated with both of the symptoms of anemia and level of hemoglobin. Education, pregnancy history, iron supplementation, information on nutritional knowledge, attitudes, and practice and dietary history regarding usual food IVintake per day were poorly appeared in study. Moreover, through focus group discussion, mostly women expressed that “they had no money, no proper food, no proper shelter, no permission for prenatal and postnatal care and husbands unquestioning loyalty create threatening environment and you (me researcher) are talking about health, think it yourself that you can find the answer, you can not find the answer”. In short it resulted in poor health condition of mothers and their children with moderate to sever anemia. It is suggested that provision of female education, income generating opportunities and utilization of health facilities and awareness about causes of consequences of anemia, knowledge and preventive measures of anemia and importance of balanced diet are appropriate measures to enhance health status of mothers and children. Awareness about suitable age at marriage, adequate adoption of pre-postnatal care, immediate attention towards heavy blood loss (abortion, menstruation), provision of iron supplements and adverse complications of pica need special attention to control over anemia status of mother and child. Government should provide proper attention to eradicate widespread prevalence of communicable diseases especially, TB, diarrhea and acute respiratory infection. Sensitization of gender issue need special attention that husband should be make aware about the health complications of different types of violence against females and children.