Safe occupational workplace and hygiene measures are important among industrial workers. Workplace related health problems are the most common of developing industry poor workers. Around the world approximately 300 million people suffer by asthma and respiration related problems. In Pakistan, there are many patients suffer by asthma and allergy complications. The highest numbers of cases have been reported in Karachi and Multan due to industrial activities and other factors. The Organic acid anhydrides (OAAs) are widely industrial chemicals used in textiles, plastics, dyes, paints, pesticides, adhesives & resin industries. The most commonly used OAAs in these industries are phthalic anhydride (PA) maleic anhydride (MA), succinic anhydride (SA), trimellitic anhydrides (TMA) and acetic anhydride (AA). These anhydrides react with proteins and form adducts (complex) contributing harmful effects of asthma and sensitization problems in the exposed workers. This study is carried out on the industrial workers of Kotri SITE Area Industrial zone located at the south west of Hyderabad, Sindh, Pakistan. Allergy is major disease with two of the most common forms with (type I allergy) symptoms such as conjunctivitis, asthma, rhinitis, and (type IV allergy) allergenic contact dermatitis found commonly among exposed workers. There is lack of occupational hygiene schemes in the SITE area, Kotri. The survey data designed questionnaire was filled from workers in the study area. The survey data has been designed with questionnaire between the different worker groups filled for workers. My study is to investigate the protein role clinically and metabolically among exposed workers from local industry workers compared with healthy controls by different analytical techniques. Spectrophotometric studies were performed on UV/Visible Lambda 35 Perkin Elmer (USA) with its computer software to confirm adducts (complex) formation of selected five OAAs (maleic anhydride(MA), phthalic anhydride(PA),succinic V anhydride(SA), acetic anhydride (AA), trimellitic anhydride (TMA) ) with two major proteins like serum albumin(SA) and hemoglobin (Hb). The SDS-PAGE Electrophoresis analysis for serum albumin and hemoglobin Proteins (dye industry & Paint industry workers) was done successfully. The clinical data and demographic properties were obtained with the help of questionnaire by local physician after taking consent. The hematological parameters (blood C.P) were performed upon industry workers as powder paints may contain excess amount of anhydrides responsible for irritation and sensitization. Biochemical analysis is an extremely important study helps in diagnosis of many diseases at earliest performed most in clinical chemistry. The blood samples analyzed for determination of LDH (Lactate Dehydrogenase), Glucose (random), Alkaline Phosphate (Alkaline Phosphatase), SGPT (Serum aspartate aminotransferase), SGOT (Serum Glutamic Oxaloacetic Transaminase), CPK (Creatine Phospho Kinase), Albumin (SA), Hemoglobin (Hb), Blood Urea, Creatinine, Total Protein, Total Cholestrol , Triglycerides, LDL Cholestrol, .HDL Cholestrol, Calcium (Ca),Chloride (Cl) and Potassium on flame photometer. The total immunoglobulin IgE was also investigated on Enzyme Linked Immunosorbant assay (ELISA) test to associate with Plasma protein role upon exposed workers and control samples. The evaluation of Blood serum samples in different groups of exposed workers was compared with controlgroups for variation. Almost 76% of workers were affected due to protein binding with hazardous anhydrides and indicated as community problem for improving the quality of life.