Spot blotch disease caused by Bipolaris sorokiniana Sacc. is a severe constraint with 30-70% estimated yield losses to wheat production in warmer regions of the world. The purpose of this study was to evaluate crude extracts of Eucalyptus camaldulensis Dehn and Eucalyptus torelliana F. Muell (Myrtaceae) for their fingicidal activity against Bipolaris sorokiniana and if they can be used as a spot blotch disease management strategy. In was observed that flowering buds are most potential part of both plant species compared to leaves and bark. The ethanol, methanol and aqueous crude extracts of E. camaldulensis flowering buds inhibited mycelial growth up to 97, 91 and 67% While; the E. torelliana extracts restricted mycelial growth of 75, 74, and 47% respectively. The E. camaldulensis flowering buds ethanol extract provided maximum inhibition zone of 29.10±0.92 with 10mg/well and minimum inhibitory concentration value of 25mg/ml. The minimum mean length of conidiophores observed (78μ) and mean width (3.63μ) for treatment of E. camaldulensis flowering buds ethanol extract at 10% concentration over control 141μ length and 7.33μ width. A range of variations in mean length of B. sorokiniana conidia observed from 49-74µ and mean width from 19-25µm having 1-7 septa and light brown colour. In control treatments mean length range from 75-77µ and width from 25-28µ. The extract treated hyphae were damaged, collapsed and thinner as compared to control treatment. In 10µl spore suspension the highest number of conidia (34±9.63) found in essential oil treatment at 0.5% concentration followed by at 1% concentration (30.±8.17) compared to distilled water treatment (53±10.34). The E. torelliana flowering buds methanol extract at 10% concentration provided the smallest fresh weight (0.026g) and dry weight (0.02g) of fungal biomass. The In-vivo effects of E. camaldulensis and E. torelliana flowering buds extracts against aggrasive isolate of spot blotch were observed on susceptible variety bhakkar 2001 of wheat. The pesticidal effect of flowering buds extracts with different spray schedules as well as different concentration was highly significant (P<0.05) for both of grain yield/spike and 1000 grain weight. The E. camaldulensis flowering bud extractsin ethanol produces highest number of grains/spike (50, 48 & 43) as compared to E. torilliana 41, 38 & 33 grains/spike underspray I, Spray II and Spray III schedule respectively at 10% concentation. In case of number of grains per spike, for each unit increase in disease severity, 0.395 units decrease observed in number of grains/spike.The R2 0.473 indicates that 47.3% variations in grains yield/spike are caused by disease severity. There is 0.269 unit’s decrease in 1000-grains weight (g) with 51.03 unit’s increase of disease severity. The relationship of disease severity was negatively correlated with yield contributing parameters. The R2 (0.574) means that 57.4% variation in 1000 grains weight is explained by predictor variable. Standard error value (3.171) represents the typical deviation between the actual 1000 grain weight and what the model predicted to be. In simple there are 3.171g typical fluctuations around the regression line E. camaldulensis flowering buds extracts were further investigated for the most active compounds. Partitioning of crude ethanol extract was done with hexanes, Toluene, Dichloromethane, ethyl acetate, n-butanol and water. Hexanes part was most active in mycelial growth assay with the maximum 86% inhibition. Two compounds, A and B were purified from hexane part through flash Column Chromatography (FCC) by silica gel, Prep-TLC and Prep-HPLC. The recovered compounds showed high antifungal activity against B. sorokiniana (spot blotch). The chemical analysis of active compounds by HPLC, MS, FT-IR and NMR revealed the presence of hydrocarbons. These compounds are >C=O carbonyl functional group containing terpenoids that form crystalline products. The results indicate that E. camaldulensis flowering buds extracts contain antifungal compounds and can be exploited as a spot blotch disease and other fungal pathogens management strategy if formulation is developed in future.