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Home > Development of Genetically Engineering Mustard Brassica Juncea for Low Viscosity Biodiesel Production

Development of Genetically Engineering Mustard Brassica Juncea for Low Viscosity Biodiesel Production

Thesis Info

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External Link

Author

Naeem, Ijaz

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9715/1/Ijaz_Naeem_Biotechnology_AUP_2015.doc

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725874814

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Indian mustard (Brassica juncea L.) is a member of the Brassicaceae family that is now becoming a global crop because of its drought tolerance, high oil content, short growing season and the ability to grow on low quality soils. The present study was aimed to reduce the viscosity of Brassica juncea oil by altering the fatty acid profile, via developing efficient and simple Agrobacterium mediated based tissue culture and floral dip methods of plant transfermation. For optimization of regeneration protocol, cotyledon explants of the Brassica juncea CV- Raya Anmol were cultured on MS medium supplemented with different concentrations of 6-Benzylaminopurine (BAP), (2, 4, 6, 8, and 10 µM) alone and in combination with 1µM of Gibberellic acid (GA3) and Naphthaleneacetic acid (NAA) separately. Maximum calli production (87%) was observed on MS medium containing 4 µM BAP only. The highest shoot induction efficiency (92%) was observed on medium supplemented with BAP (4 µM) and NAA (1 µM). For rooting, Indole-3-acetic acid (IAA) (6µM) and Kinetin (Kin) (1µM) was found as the best combination. A binary vector containing the EaDAcT (E.alatus diacylglycerol acetyltransferase) gene under the transcriptional control of a glycinin promoter and with a basta selection marker, was introduced into A. tumefaciens strain GV3101 via electroporation. An efficient tissue culture and In planta transformation was developed for B. juncea. In tissue culture transformation, cotyledon explants were used while for floral dip, plants at early stage of flowering with and without vacuum desiccator were used. The basta resistant putative transgenic plants were selected and further confirmed by PCR. The vacuum infiltration was found more efficient (0.85%) as compared to the normal floral dip method (0.15%).The developed transgenic B. juncea seeds showed altered TAG fatty acid composition with enhanced level of oleic acid (from 41% to 63%) and reduced eurcic acid level (from 13.7% to 2.53%), which is an ideal composition of fatty acids in oil to be used as biodisel. The developed protocols could be used to accumulate unusual acTAG in B. juncea seed, providing a direct way of biodiesel production from plant oil. Such results will be useful for the development of B. juncea as an alternative source of energy.
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ڈاکٹر شہزاد احمد کی نعتیہ شاعری

ڈاکٹرشہزاد احمد کی نعتیہ شاعری
نعت کی مختصر روایت
عربی میں نعت کے معنی ’’وصف‘‘ کے ہیں لیکن اُردو میں اس کا استعمال حضور اکرم صلی اللہ علیہ و آلہٖ وسلم کی ستائش وثنا کے لیے مخصوص ہے۔(۱)اصطلاح میں ہر وہ نثر پارہ یا منظوم کلام جو رسول کریم صلی اللہ علیہ و آلہٖ وسلم کی مدح میں ہو نعت کہلاتا ہے، نعت گوئی وصف محمود کا دوسرا نام ہے۔ اس بارے میں ڈاکٹر رفیع الدین اشفاق رقم طراز ہیں:
’’نعت کے معنی یوں تو وصف کے ہیں لیکن ہمارے ادب میں اس کا استعمال مجازاً حضرت رسول سیدالمرسلین صلی اللہ علیہ و آلہٖ وسلم کے وصف محمودو ثنا کے لیے ہوا ہے جس کا تعلق دینی احساس اور عقیدت مندی سے ہے۔‘‘(۲)
نعت کا آغاز اللہ رب العزت نے خود کیا اور انبیاء ورُسل کو نبی آخر الزماںصلی اللہ علیہ و آلہٖ وسلم کی آمد کی نوید سنائی۔ پھر قرآن مجید خود رسول کریم صلی اللہ علیہ و آلہٖ وسلم کے اوصاف حمیدہ اور سراپامبارک کے بیان سے مزین ہے۔ یہ سلسلہ قبل ازاسلام سے جاری ہے۔ تبع حمیری جو شاہ یمن تھا، اُس کے اشعار زبان زد عام ہیں ۔ راجا رشید محمود کے مطابق:
’’اولین نعت کی حقیقت یہ ہے کہ ہمارے آقا و مولاصلی اللہ علیہ و آلہٖ وسلم کے اولین نعت گو تبان اسعدبن کلی کرب تھے جنھیں تبع، شاہ یمن کہا جاتا ہے اور وہ حضورصلی اللہ علیہ و آلہٖ وسلم سے کم ازکم سات سو سال پہلے ہوئے ہیں۔‘‘(۳)
بعدازا سلام عرب میں پہلی نعت جناب ابوطالبؓ نے سردارانِ قریش کے سامنے کہی۔ یہ نعت قصیدے کی صنف میں تھی۔ اس کے بعد حضرت حسان بن ثابتؓ، عبداللہ بن رواحہؓ، کعب بن مالک انصاریؓ اور کعب بن زہیرؓ کاروان نعت کے وہ خوش نصیب شعرا ہیں جنھوں نے حیاتِ رسولؐ...

تأثير الفقر على حياة الشاعر الجاهلي: دراسة تحليلية

There is no doubt that poverty is one of the major phenomenon which destroys the entity of the human society and it is also one of the obstacles that prevents the ability and talent of humans to create and innovate in a suitable environment, it also causes the ignorance, mental and social tension, and it also desists human being to strengthen his family ties and make well settled position in the society, it affects adversely the life and mind of humans and forced him to commit crimes and violations in the society. We see that this phenomenon has affected the life of pre-Islamic poet and compelled him on raiding and robbery, so in this article we have tried to highlight the impact of poverty on the life of pre-Islamic poet.

Assessment of Health Status of Captive Pheasants Through Blood Profiling and Fecal Parasite Analysis in the Punjab Province

The present study was designed to evaluate the assessment of health status through blood profiling and fecal parasite analysis in three species of pheasants in captive facilities of Punjab.The total 75 blood and fecal samples were collected to determine hematological parameters and prevalence of parasites. Hematological parameters including Total Red Blood Cell Count, Hemoglobin, Packed cell volume, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration, Total White Blood Cell Count, Platelets, Granulocytes, Lymphocytes, Monocytes, and Neutrophils, and Plasma Chemical parametersUrea, ALT, AST, Creatinine, Iron, Total Protein and Albumin were calculated. The comparison of mean value of all the parameters among male and female in silver pheasants showed that HGB, MCV, MCH, MCHC, Urea, AST, Iron and Total protein was differ highly significantly (P<0.01) and RBCs and creatinine was significantly (P<0.05). In male and female ring necked pheasants RBCs, HGB, MCH, Neut, Urea and Iron was differ highly significantly (P<0.01) and HCT, MCHC, PLT, AST and creatinine was significantly (P<0.05). In male and female green pheasants MCHC and ALT was differ highly significantly (P<0.01) and GRAN, Neut and creatinine was significantly (P<0.05), and all the other parameters in male and female of three species of pheasants were differ non-significantly (P>0.05). The prevalence of endoparasite in all three species was ranged from 36%-56%. The highest prevalence recorded in ring-necked pheasants 56%, followed by silver pheasants 44% and green pheasants 36%. The overall prevalence of endoparasites was 45.3%. The prevalence of endoparasite among all the nine different facilities differ highly significant (P<0.01) and its range from 0-83.3%. The comparison between male and female parasites of all three species shows that females were (48.8%) more infected as compared to male (40%). The total five species of endoparasites in all pheasants were recorded i.e. Ascradia galli (28%), Capillaria annulate (38.2%), Heterakis gallinarum (47%), Strongyloides avium (17.6%) and Eimeria sp (20%). The present study provides base line value of hematology and plasma chemistry of apparently healthy pheasants in captivity and can be used as reference values to improve the management practices of these species. The high prevalence of parasites needs to be re-evaluated to assess efficacy and possible nematodicide resistance of these parasites.