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Development of Microbial Proteases for Industrial Applications

Thesis Info

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Author

Bashir, Fareeha

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13561/1/Fareeda_Bashir_Biochemistry_HSR_2018_UAF_31.07.2018.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725879545

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Enzymes are the biological macromolecules that speed up the conversion of substrates into products by lower the activation energy of the reacting species. Protein hydrolyzing enzymes (proteases) consist of the major assembly of enzymes being used in industry with a diversity of applications in the food, detergents, leather and meat industries. Due to increasing industrial demands, interest has been growing in microbial proteases for application in different commercial scale processes. Thus, there is need to explore new sources of alkaline proteases. The aim of this research was to identify potential protease producers. Out of six microbial strains Bacillus licheniformis and Aspergillus flavus were found to be the best producers of protease. To enhance the production of proteases by selected strains, different physical parameters, e.g., pH, temperature, inoculum size and fermentation time, were optimized by applying response surface methodology (RSM) under central composite design (CCD). Similary, nutritional parameters such as carbon and nitrogen source were also optimized and the influence of C:N ratio and metal ions on the yield of proteases, were also determined. The proteases obtained were purified by ammonium sulphate precipitation followed by dialysis, ion exchange and gel filtration chromatography methods. The purified proteases were immobilized by synthesizing cross-linked enzyme aggregates (CLEAs) and by optimizing the different parameters for CLEAs formation by RSM under CCD. SEM (scanning electron microscopy) of CLEAs were performed for the presence of protease in CLEAs. Free protease and CLEAs were compared by determining the effect of substrate (casein) concentration, pH and temperature. Free and CLEAs of proteases were used for industrial applications. Concerning industrial applications, alkaline proteases efficiently remove blood stain from fabric, remove the gelatin layer from used X-ray film, degrade animal bones protein, clot milk and dehair animal hides.
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سفر طائف

سفرِ طائف:
اللہ تعالیٰ نے حکم دیا کہ ’’ وَ اَ نِذِ ر عَشِیرَ تَکَ الا َ قّرَبِینَ اپنے قریبی رشتہ داروں کو اسلام کی دعوت دو‘‘ اور یہ بھی فرمایا وَتَوَ کلَ ّعَلیٰ العَزیزِ اِلرَّ حِیمِ ’’ بھروسہ کرو خدا قادر و رحیم پر اَلَّذِی یَرَ ک حِینَ تَقُومُ جو تم کو دیکھتا رہتا ہے‘ جب تم کھڑے ہوتے ہو وَتَقَلُّبِکَ فِی السَّجِدِینَ اور نمازیوں کے ساتھ تمھاری نشست و بر خاست کو وہ دیکھتا رہتا ہے‘‘ (الشعرائ۔۲۱۷۔۲۱۸)۔ جب مکہ کی فضا مزید بگڑتی ہے۔ظلم و ستم اور جبر و تشدد میں اضافہ ہوتا ہے تو آپؐ کارِ تبلیغ کو جاری رکھنے کے لیے طائف کو منتخب کرتے ہیں۔ طائف مکہ مکرمہ سے ساٹھ میل دور مشرق کی طرف واقع ہے۔ پہاڑی علاقہ مگر سر سبز و شاداب ہے۔قدرتی چشمے اور قسم قسم کے پھلوں اور میووں کے باغات ہیں۔ سرولیم میور یورپ کے متعدد سیاحوں کے اقوال طائف اور اس کے میووں، پھلوں ، خصوصاََ انگوروں اور گلاب کے پھولوں کی تعریف میں نقل کرتا ہے۔آپؐ مکہ سے طائف کا سفر پیدل کرتے ہیں۔ آپ ﷺکے ساتھ آپ کا آزاد کردہ غلام سیدنا زید بن حارثہؓ ہیں۔راستہ کٹھن ہے مگر راستے میں قبائل کو اسلام کی دعوت دیتے جاتے ہیں۔ قبیلہ بنو بکر کے ہاں جاتے ہیں لیکن وہ ٹھہرنے نہیں دیتے۔قبیلہ قطحان والے بھی بد سلوکی سے پیش آتے ہیں۔ بالآخر آپؐ طائف کا عزم کرتے ہیں۔دس روز قیام کیا۔بنو ثقیف کے سرداروں نے نا روا سلوک کیا۔آپؐ نے ایسے سڑے گلے اور روکھے پھیکے جواب ہی نہیں سنے بل کہ یہ سردارا ن اوباش اور آوارہ لڑکوں کو پیچھے لگا دیتے ہیں۔وہ تالیاں بجاتے،گالیاں دیتے،آوازے کستے حتیٰ کہ پتھروں کی بارش کردیتے ہیں۔ ان پتھروں سے آپ لہو لہان ہو گئے۔ آپﷺ کے نعلین مبارک خون سے بھر گئے۔آپؐ کا غلام پتھروں...

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Improving Writing Skill with Special Reference to Management Sciences at BBA Leve

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