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Development of Novel Radiopharmaceutical for Cancer Diagnosis and Sentinel Node Detection

Thesis Info

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Author

Dar, Ume Kalsoom

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2012

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1153

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725883446

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This study was undertaken to develop the novel radiopharmaceuticals for cancer diagnosis, sentinel lymph node detection (SLND), and brain imaging. Biomolecules have great importance in living systems. Significantly, developed pathway is available for transport and utilization of biomolecules and by taking the advantages of these naturally developed pathways. In this study, the biomolecules like triglycine, 1-azido-1-deoxy-β-D glucopyranoside and folate azide were utilized for various medicinal applications. Biomolecule conjugates were developed by using click chemistryand cyclopentadienyl chemistry (Cp) approach. The spectrophotometric biomolecule complexes techniques. conjugates were were By characterized using radiolabeled the and by using various [ 99m Tc(H 2 O) 3 (CO) 3 ] + evaluated as core, potential radiopharmaceuticals for diagnostic study. The biodistribution of [ 99m Tc(CO) 3 - click-1-azido-1-deoxy-β-D glucopyranoside] and [ 99m Tc(CO) 3 -folate azide] complexes present them as potential tumor scintigraphic agents. Second approach for cancer diagnosis was to radiolabel the anticancer drugs, e.g., 5-fluorouracil and methotrexate with technetium-99m ( 99m Tc) and evaluate them as potential tumor diagnostic agents. Normal biodistribution and tumoral uptake was studied in Swiss webster mice. The results suggest that they might be sucessful tumor diagnostic agents for future use. 99m Tc-5-flurouracil was also applied to the breast cancer patients after getting approval from local ethical committee of INMOL. The sucessful uptake of the drug in tumor tissues with low background ratio presents this drug as a sucessful cancer diagnostic agent. Sentinel Lymph Node Detection (SLND) is of great importance for early stage diagnosis of cancer. All sentinel lymph node imaging agents were studied for their uptake in various body organs, popliteal node, iliac node and popliteal extraction.In this study, we developed rhenium sulfide collidal nanoparticles and xviiiAbstract radiolabeled them with 99m Tc. The sucessful uptake of the drug in sentinel node within 15 min of post injection, presents it as a sucessful candidate for sentinel lymp node detection. Various dextran derivatives were radiolabeled with 99m Tc(CO) 3 and studied for sentinel lymph node detection. The drugs reached the first node within 10 min of post injection. DPZM-8 was the most successful candidate for sentinel lymph node detection among all dextran derivatives studied, so far. 99m Tc-labeled amine thiophene and 99m Tc(CO) 3 -Cp-triglyicne were evaluated as potential brain imaging agents. Amine thiophene is a schiff base which was directly labeled with 99m was radiolabeled with Tc while Cp-triglycine is a biomolecule conjugate which 99m Tc via isolink kit. Normal distribution study in rabbits suggests them as successful candidates for brain scintigraphy.
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الباب الثالث: الشاعرة المعروفة بروين شاکر

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ٹریڈمارک، کاپی رائٹ اور حقوق کی خرید و فروخت کا شرعی جائزہ

There are many things which would not be bought or sold in earlier ages but these days they are considered great wealth and precious capital and their transaction is in vogue and  they are titled as  rights. For instance, literary copyright and copyright of music and trade mark etc. Now the need of the hour is to understand the Islamic solution of the trade of these different kinds of rights. This article reviews the copyright issue in the light of islam.

Diversity of Soil Inhabiting Mites in Different Ecological Zones of Punjab, Pakistan.

Soil is a very complex ecosystem comprising of interaction of different arthropods including mites. Soil mites are a highly diverse group, having important role in agricultural productivity by increasing the soil fertility through the process of decomposition, mineralization and having good potential for biological control of different pests. They have potential to be used as indicators for soil quality and sustainable agriculture. Previously, no work has been done on the diversity of soil inhabiting mites from Pakistan. The project present in hand with the objectives to study diversity of soil inhabiting mites in different ecological zones of Punjab and to study the impact of different soil parameters and soil disturbance for crop production on the soil mite diversity. For this purpose, ten localities from four different ecological zones of Punjab were selected. Collection of soil samples was done on bimonthly interval for a whole year from these localities from two different types of soils i.e. undisturbed/uncultivated (soil type I) and disturbed/cultivated (soil type II) type of soil. Total 8851 specimens were collected with an average of 24.59 specimens/sample which is low as compared to other part of the world. The Cumulative and locality wise individual based rarefaction curves attained asymptotic level, except in case of soil type II of district Faisalabad and T.T Singh. The Shannon diversity (Hꞌ) value represents that the diversity of soil type I (Hꞌ= 2.71) was high as compared to soil type II (Hꞌ=2.51) throughout the study period. Irrigated plain was more diverse as compared to other zones in both types of soils i.e. soil type I (Hꞌ=2.81) and II (Hꞌ=2.65). In Mesostigmata, soil type I was more diverse (Hꞌ=1.67) as compared to soil type II (Hꞌ=1.26) while, in Oribatida, soil type I was reported more diverse (Hꞌ=1.77) as compared to soil type II (Hꞌ=1.47). In case of richness, soil type I has more rich (16.59) as compared to soil type II (12.01). Similarly, in soil type, I, mean abundance (22.70) was higher as compared to soil type II (11.56) while irrigated plain zone has more mean number of specimens (28.59) in soil type I as compared to soil type II (13.03). Oribatida was the most prominent group with 50% followed by Mesostigmata 38%, Prostigmata 8% and Astigmata 4% individuals. Ten families were reported from Mesostigmata, followed by Oribatida with 9 families, Prostigmata 5 and Astigmata with only one family. The cumulative rank abundance curves for soil type I and soil type II suggested that presence of soil mite families and numbers of individual varies from different localities and time in various months. Week correlation of N and pH were reported with soil mites. Other soil parameters had no impact on the abundance of soil mites. Maximum temperature, rainfall and humidity had weak significant correlation with mites’ abundance.