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Home > Development of Serodiagnostic Test for Tuberculosis by Using Mycobacterium Tuberculosis Specific Antigens

Development of Serodiagnostic Test for Tuberculosis by Using Mycobacterium Tuberculosis Specific Antigens

Thesis Info

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Author

Irum Nawaz Awan

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/950

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725888408

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The effective removal and control of tuberculosis (TB) disease can be achieved with early and accurate diagnosis. It accounts for a majority of deaths and loss of health status thus damaging the economy. The present diagnostics for TB are not very effective as their sensitivity and specificity are low. Therefore the tests with more diagnostic value need to be developed. Thus a study was planned to develop an indigenous technology by exploiting biotechnology tools, and a new emerging technique i.e. multiplex microbead immunoassay (MMIA). Six potential recombinant antigenic genes: ag85a, ag85b, ag85c, cfp-10, esat-6 and hspx of Mycobacterium tuberculosis (M. tuberculosis) were selected for this purpose and respective genes were transformed into expression strain Bl21DE3pLysS for overexpression of proteins. Expression of each antigen was optimized for various conditions like concentration of isopropyl beta-D-1-thiogalactopyranoside (IPTG), time and temperature. Expression of protein was then confirmed by Western blotting. After confirmation, proteins were overexpressed in bulk cultures and purified by using immobilized metal affinity chromatography (IMAC) by using Histidine-tag (His-tag). The purified proteins were quantified and used to coat on microbeads at different concentrations and were used for analysis of collected blood samples. The blood samples of TB patients and healthy controls were collected from Federal TB Centre, Rawalpindi, from the students of Pir Mehr Ali Shah - Arid Agriculture University Rawalpindi (PMAS-AAUR), Rawalpindi, Punjab, Pakistan and healthy controls from USA. The collected human blood samples were divided as tuberculin skin test negative (TST -ve) healthy controls, from Pakistan and USA (group 1), TST positive (group 2), reactivated TB patients xx(group 3) and time points of active TB patients who were diagnosed and were under treatment (group 4). The coated microbeads were then used to analyze the presence of antibodies against M. tuberculosis in the collected blood samples. The results of group 1, Pakistan and USA group (TST negative) showed in general the absence of antibodies against any of the six antigens used in the MMIA. In the group 2 (TST positive), low median fluorescence intensity (MFI) values were detected against all antigens. Further in group 3 (reactivated TB patients) highest MFI values were observed against all antigens whereas in group 4 (active TB patient time points) MFI values were higher than group 2 but lower than group 3. This shows MMIA is very specific in detection of TB. Therefore, based on this it may be concluded that these antigens can be used to develop MMIA. However, use of more antigens and standardization is required.
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ڈاکٹر بھگوان داس

ڈاکٹر بھگوان داس
اسی مہینہ ہندوستان کی ایک اور نامور شخصیت ڈاکٹر بھگوان داس نے انتقال کیا، وہ اپنے دور کے مشہور صاحب علم و قلم اور درویش صفت صوفی مشرب فلسفی تھے، فلسفہ اور تصوف پر ان کی بڑی گہری نظر تھی، اس پر انگریزی میں ان کی کئی تصانیف ہیں، اسلام سے پوری طرح واقف اور اسلامی تصوف سے خاص ذوق رکھتے تھے، عقیدۃً موحد اور اپنی تہذیب و معاشرت میں پرانی تہذیب و شرافت و وضعداری کا نمونہ تھے، اپنے مسلمان دوستوں کو جب خطوط لکھتے تھے تو اپنا نام عبدالقادر لکھتے تھے اور کہتے تھے کہ بھگوان داس اور عبدالقادر کے معنی ایک ہیں، بمبئی کے گورنر سری پرکاش صاحب کے والد تھے، ان کو شرافت و وضعداری اپنے والد ہی سے ترکہ میں ملی ہے،ڈاکٹر بھگوان داس کی موت سے ہندوستان کی ایک بڑی علمی و تہذیبی یادگار مٹ گئی۔ (شاہ معین الدین ندوی، اکتوبر ۱۹۵۸ء)

 

Pengaruh Konsep Diri dan Disiplin Belajar terhadap Motivasi Belajar Peserta Didik di Sekolah Menengah Pertama Negeri 4 Palu Pada Mata Pelajaran Pendidikan Agama Islam

The purpose of this study is to find out the significant effect of self-concept on learning motivation of VII grade students in SMP Negeri 4 Palu on Islamic Religious Education subjects, (2) knowing the effect of learning discipline on learning motivation of VII grade students at SMP Negeri 4 Palu in the eyes of Islamic Religious Education subjects and (3) determine the effect of self-concept and learning discipline on learning motivation of grade VII students in SMP Negeri 4 Palu on Islamic Religious Education subjects. This study uses a quantitative approach. The population in this study were 220 class VII students of SMP Negeri 4 Palu. The sample was determined using proportional random sampling technique using a formula developed by Isaac and Michael, so that a sample of 135 students was obtained. Primary data were obtained using a questionnaire. Data analysis techniques include descriptive analysis, and regression tests using simple regression and multiple regression. The results showed that (1) There was a significant influence between self-concept on learning motivation of VII grade students in SMP Negeri 4 Palu on Islamic Religious Education subjects, with a significance level of 5%, the magnitude of the effect of self-concept variables on students' learning motivation was 0, 615 means having a high influence (2) There is a significant influence between learning discipline on learning motivation of VII grade students in SMP Negeri 4 Palu on Islamic Religious Education subjects, with a significance level of 5%, the magnitude of the effect of the discipline of learning variables on motivation student learning is 0, 565, (3) There is a significant influence between self-concept and learning discipline on learning motivation of grade VII students in SMP Negeri 4 Palu on Islamic Religious Education subjects, with a significance level of 5%, the high influence of concept variables self and discipline of learning towards learning motivation of VII grade students of SMP Nege ri 4 Palu in Islamic Religious Education subjects is 0, 615. The contribution of self-concept variables and learning discipline to learning motivation variables is R2 x 100% or 0, 6152 x 100% = 37.9%, the remaining 62.1% is determined by other variables outside of this study include the level of interest, talent, attention, parenting parents and others.

Using Text Processing Techniques for Linking News Stories for Digital Preservation

The news generation in the digital environment is no longer a periodic process with a fixed single output like printed newspaper. The news are instantly generated and updated online in a continuous fashion. However, because of different reasons like the short lifespan of digital information and speed of generation of information, it has become vital to preserve digital news for the long-term. Digital preservation includes various actions to ensure that digital information remains accessible and usable, as long as they are considered important. Libraries and archives preserve newspapers by carefully digitizing collections as newspapers are a good source of knowing history. The lifespan of news stories published online vary from one newspaper to another, that is, from one day to a month or even more. Though a newspaper may be backed up and archived by the news publisher or national archives, in the future it will be difficult to access particular information published in various newspapers about the same news. The issues become more complicated if a story is to be tracked through a multi-lingual archive of many online newspapers, which require different access technologies. Based on prior studies, a ten step systematic approach is introduced for web preservation, which lead to create an effective web archive and followed to create the intended digital news stories archive using digital news stories extractor. Initially, the archive is enriched with three English newspapers, enhanced to ten online newspaii pers and then upgraded to dual language (Urdu and English) news articles archive, extracted from fifteen online news sources. The news stories archive preserve about 360 Urdu and 850 English news articles, periodically crawled every second day using digital news stories extractor tool. The main goal of the dissertation was to link digital news stories duration preservation using text processing techniques. To achieve the goal, the formulated text processing similarity measures are applied for linking two types of news articles, that is, to link English news (English-to-English) and dual languages (Urdu-to-English) news articles in the archive. To linking English news articles in the archive, the study proposed five contentbased similarity measures that find similarity based upon news content features and link news articles during preservation. The measures compute similarity value among news articles based on features like number of terms, named entities, named entities position, title terms, and position of terms in the titles, etc. The measures are evaluated on to same news articles sets, of different size and compared with human based judgment in order to evaluate the accuracy and assess the effectiveness, worth and significance of designed similarity measures for linking English news articles. The results showed that the proposed measures presented are feasible for linking English news articles in the news stories archive. The selection of measure depends upon the performance of that measure in a specific category, for example, a measure can perform better on a category “Opinion”. All the proposed measures are evaluated for six categories of news articles and the results are mutually compared with two known text based similarity measures to assess the effectiveness and appropriateness of proposed measure in the best fitted scenario. The pre-processing step in any web preservation project is of utmost importance because the intensions are to archive the targeted contents, especially, in a language which doesn’t have any sophisticated tools and techniques. To link dual languages news articles in the archive, Urdu news articles needs extensive pre-processing, which leads to create an Urdu bag of words and dictionary containing 50502 words and 78739 pairs of Urdu words with English meanings respectively. The study proposed five content-based similarity measures that find similarity based upon news content features and link Urdu-to-English news articles during preservation. The measures are applied to the same news articles sets, of different sizes and mutually compared. The results showed that three of the proposed measures presented appreciable results for linking dual-lingual news articles in the archive, which can be improved by improving the structure and contents of the Urdu dictionary. In summary, the performance of different measures has been evaluated individually for linking digital news articles in the digital news story archive to make sure the accessibility of these news articles in the future from this enormous collection.