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Home > Development of Therapeutic Foods With Stevia Rebaudiana Bertoni: Exploring its Use to Determine Anti-Diabetic and Hypolipidemic Potential

Development of Therapeutic Foods With Stevia Rebaudiana Bertoni: Exploring its Use to Determine Anti-Diabetic and Hypolipidemic Potential

Thesis Info

Access Option

External Link

Author

Uswa Ahmad

Program

PhD

Institute

Government College University

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Food Science & Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11428/1/uswa%20ahmad%20food%20nutrition%202018%20gcu%20faisalabad%20prr.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725892246

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Stevia rebaudiana Bertoni is considered as natural and non-toxic sucrose alternative that has ability to manage obesity and its associated diseases. Moreover, it is safe for human health and does not produce side effects. For the purpose, stevia leaves powder was characterized and stevioside (major glycoside in stevia due to which its taste is sweet) were extracted through different solvents (methanol, ethanol and water), purified and quantified by HPLC. Stevia extracts were also analyzed for physicochemical parameters.Regarding chemical composition, stevia leaves powder is rich source of both macronutrients and micronutrients. Ethanolic extract of stevia had high amount of antioxidants and stevioside as compared to methanolic and aqueous extracts of stevia. pH of ethanolic extract was less while total soluble sugars and total sugars were more than other extracts of stevia. In the product development phase, stevia leaves powder was utilized in the preparation of muffins and pure stevioside from aqueous stevia extract was incorporated in peach ready-to-serve beverages as sugar substitute. Both stevia muffins and stevioside from aqueous stevia extract containing RTS peach beverages were analyzed for phytochemical, physical and sensory analyses. The well-designed drinks were also subjected to all these analysis during two months storage period. Stevia improved the nutritional and antioxidant profile of both products. The physical and organoleptic evaluation parameters were affected significantly in muffins and beverages except pH and acidity. The antioxidant and physicochemical properties of stevioside containing RTS peach drinks were substantially affected and organoleptic evaluation parameters remained acceptable during storage. Animal studies were performed by varying the diets to investigate the anti-diabetic and hyperlipidemic potential of stevioside. In study I diabetes was induced in rats and then stevia aqueous extract was given to them and their anti-diabetic effect was compared with normal and diabetic control. In study II hypolipidemic potential of stevia aqueous extract was determined and compared with normal and hyperlipidemic controls. Stevia aqueous extract caused in momentous drop in body weights in both diabetic and hyperlipidemic rats. In the diabetic rats stevia significantly decreased the random, fasting blood glucose levels and HbA1c and elevated the insulin and glycogen contents. The total cholesterol, total triglycerides, low density lipoproteins, very low density lipoproteins levels and LDL/HDL ratio significantly decreased and high density lipoprotein level improved in hyperlipidemic rats taken stevia aquatic extract. ALT, AST and ALP significantly decreased and total weights ratios of body organs remained unaffected in both studies. Fromtheexistingexploration,itisassumedthat stevia aquatic extract is activein contradiction ofobesity,diabetes and hyperlipidemia.
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لسان فسوں

لسان فسوں
فلک کی جالیوں سے لگ کر رونے والی۔۔۔!
میری رگوں میں خون بن کر دوڑتی رہتی ہے
میں زمین پر۔۔۔اسی کے خال و خد بناتے ہوئے!
دستِ حنائی کے لمس۔۔۔!
دلفریب لہجے کے سحر میں ڈوبے ہوئے۔۔۔!
تلمیحاتِ خمسہ کی ساری روایتیں۔۔۔!
غیلان،عِلاف،کندی۔۔۔!
فرزند سینا اور فارابی کی آیتیں بھی جانتا ہوں
طلوع سحر کا رازی۔۔۔جب اک وعدہ شام ہونے لگتا ہے
پھر میں موسم زلیخا کا۔۔۔
اک خوب صورت بہانہ بننے لگتا ہوں
سرخ سبز پرندوں کا ورد زباں ہونے لگتا ہوں

جب بھی فسوں ساز شاموں کی بات ہوتی ہے
زیتون و صندل۔۔۔!
سفید کبوتروں جیسے صحیفے پڑھنے لگتی ہیں
شاخوں پر پھول پتے نازل ہونے لگتے ہیں
جنوں کی روشنی میں جناب عنترہ اور عبلہ سے بھی ملاقات ہوتی ہے
جب طاق لمحے۔۔۔مجھ سے گلے ملتے ہوئے گزرتے ہیں
پھر تخلیقات عشق کا سنہری زمانہ بننے لگتا ہوں
سرخ سبز پرندوں کا ورد زباں ہونے لگتا ہوں

لسان فسوں جب دشت میں!
گلابی صحیفوں سے آیتیں تلاوت کرنے لگتی ہے
نشاط تعبیر کی وادیوں میں خوشبو۔۔۔!
شاداب جزیرں میں چاندنی رقص کرنے لگتی ہے
دل دھڑکنے لگتے ہیں
بزرگ جوگیوں سے۔۔۔!
چنبیلی کے سائے میں مناظرہ کرنے لگتے ہیں
لذت زخم کی تفسیر کرتے ہوئے!
جب سیفو،عبلہ اور ماریہ قندیل روشن کرتی ہیں
پھر قلندرانہ رقص کرتے ہوئے۔۔۔میں پروانہ بننے لگتا ہوں
سوسن و نسترن کی قسم!
میں لذت خمار کا تعارف غائبانہ بننے لگتا ہوں
سرخ سبز پرندوں کا ورد زباں ہونے لگتا ہوں

Assessment of Blood Glucose and Calcium Concentrations Among healthy female volunteers Blood glucose & calcium in females

Abstract: Hypoglycemia and Hyperglycemia are associated with a number of complications including kidney damage, neurological damage, cardiovascular damage,  damage to the retina or damage to feet and legs,  lethargy, impaired mental functioning and irritability. The most abundant mineral in the body is calcium. Its abnormal levels also associated with multiple disorders. Objectives: The aim of this study was to assess the blood glucose and calcium levels in healthy female volunteersMethods: A cross sectional study was conducted at the Sir Ganga Ram Hospital, Lahore, over a period of 6 months, after obtaining the ethical approval from the The University of Lahore. A total number of 60 females were enrolled through consecutive sampling technique. Females of all age groups were included. All of the females had gone through routine history taking and lab investigations for the assessment of blood glucose an calcium concentrations. Results: In this study out 60 female participants, 33.3% were between ages of 15-30 years, 33.3% were between age of 31-50 years and remaining 33.3% were above 50 years old. All females of age group 15-30 years had normal glucose levels while single case of age group 15-30 years had abnormal calcium level. The results indicated that females of above age 50 years had low calcium and high glucose levels due to poor nutrition and less physical activity. Conclusions: The results of the current study showed that females with increasing age should intake food enriched in calcium and low glucose and adopt a physically active life style to manage normal blood glucose levels.

In - Vitro Modulation of Human Glioblastoma Cells U87 by N- 2 Hydroxy Pheny Acetamide

Glioblastoma multiforme (GBM) is the most common and malignant primary brain tumor. It accounts for more than 60% of all brain tumors in adults. Treatment options available for malignant gliomas include gross total resection, radiation therapy and chemotherapeutics, e.g., temozolomide, carmustin or carboplatin. In spite of the variety of modern therapies against GBM, it is still a deadly disease with extremely poor prognosis. Patients usually have a median survival of approximately 14-15 months. The development of new therapeutic strategies for the management of gliomas is therefore crucial. The present study is designed to analyze the therapeutic potentials of anti-inflammatory compound N-(2-hydroxyphenyl) acetamide (NA-2) and anti-hypertensive drug Verapamil (VP) in the treatment of GBM as well as their combine effect with standard drug Temozolomide (TMZ) on U87 human glioblastoma cells. MTT assay was used to determine the growth inhibitory effect of NA-2, VP and TMZ. It was observed that these three drugs inhibited growth of U87 in dose dependent manner. The IC50 doses of NA-2, VP and TMZ were found to be 1.7 mM, 0.45 mM and 0.134 mM respectively. To find out whetherapoptosis is involved in growth inhibition, cells were treated with IC50 doses of these drugs and the effect was observed on morphology, chromatin condensation and DNA fragmentation using phase contrast microscopy, DAPI staining and TUNEL assay respectively. In all three treatment groups cells showed apoptotic morphology, chromatin condensation and DNA fragmentation which are hallmarks of apoptosis. Apoptosis is a process of programmed cell death regulated by pro-apoptotic (Bax) and antiapoptotic genes (Bcl-2). Deregulation of these genes is found to be linked to gliomagenesis. Hence an increase in Bax/Bcl-2 ratio favors the process of apoptosis. Caspase-3 is also an important protein that acts downstream to Bax/Bcl-2 and involved in the execution of apoptosis. Keeping in mind the importance of these apoptotic markers we also studied the effect of NA-2, VP and TMZ on these markers to find out their possible mechanism of action. Cells were treated with IC50 doses of these drugs for 24 hrs and RT-PCR was performed to see the mRNA expression of these markers in vehicle control and the three treatment groups. It was observed that after 24 hrs of treatment both NA-2 and VP downregulated Bcl-2 expression while TMZ has not shown any significant effect on the expression of Bcl-2 as compared to vehicle control. Baxand caspase-3 expression was found to be significantly up-regulated in NA-2, TMZ and VP as compared to control. Beside these proapoptotic and anti-apoptotic proteins we also studied the effect of these drugs on the expression of two other cellular markers that are involved in growth and proliferation of glioma i.e. HIF-1α and VEGF. Both NA-2 and VP inhibited the expression of VEGF and HIF-1α which is a transcriptional regulator of VEGF. While TMZ has not shown any significant effect on these markers. Next, the effect of NA-2 and VP was studied on growth inhibition after combining them with TMZ in various different concentrations. Coefficient of drug interaction (CDI) was also calculated. It was found that combination of 0.33 mMNA-2 with 0.1 mM of TMZ and 0.025 mM of VP with 0.1 mM of TMZ produced synergistic effect on growth inhibition with CDI value < 1. Combination doses that produced synergistic growth inhibitory activity were used for further studies. TUNEL assay was used to detect apoptosis in combination treatment group (TMZ + NA-2 and VP + TMZ) and individual drug treatment groups i.e NA-2 (0.33 mM), TMZ (0.1 mM) and VP (0.025) using doses that produced synergistic effect. Results of TUNEL assay revealed that even low doses (mentioned above) of NA-2, TMZ and VP induced apoptosis and this apoptotic effect was more pronounced in combination treatment groups (TMZ + NA-2 and VP + TMZ) as compared to control and individual drug treatment groups. To determine the possible mechanism of action involved in synergism we studied the effect of NA-2, TMZ and VP individually and in combination (TMZ + NA-2 and VP + TMZ) on the same molecular markers that we studied at IC50doses of these drugs. Expression was analyzed at both mRNA and protein level using RT-PCR and Immunocytochemistry. NA-2, TMZ and VP (non significant mRNA) up-regulated the expression of Bax at both mRNA and protein level andthe expression was more pronounced in combination treatment group TMZ + NA-2 as compared to individual treatment of NA-2 and TMZ. In case of VP + TMZ no further increase in expression was observed as compared to TMZ only. In contrast to BAX, Bcl-2 was found to be down regulated after treatment with NA-2 and VP as compared to control while TMZ had no significant effect on the expression of Bcl-2. Moreover No further significant down-regulation of Bcl-2 was observed at protein level when NA-2 and VP alone treatment groups were compared with TMZ + NA-2 and VP + TMZ respectively. Increase in Bax expression by NA-2, TMZ and VP and down-regulation of Bcl-2 by NA-2 and VP only leads to dramatic increase in Bax/Bcl-2 ratio and shifted the equilibrium of cells towards apoptosis. Apoptosis was further confirmed by analyzing active Caspase-3 expression. NA-2, TMZ and VP treatment also increased active caspase-3 expression and the expression was highest in combination treatment groups where Bax/Bcl-2 ratio and apoptosis was also highest as compared to control and individual treatment of the drugs. Here we concluded that the synergistic growth inhibition that was observed in combination treatment group may in part be related to increase in apoptosis. The expression of HIF-1α and VEGF was alsoanalyzed in combination treatment and found similar results that were observed at IC50 doses. Both NA-2 and VP inhibited the expression of HIF-1α and VEGF while TMZ had no effect on the expression of both these marker. No further significant down-regulation was observed when NA-2 + TMZ and VP + TMZ were compared with NA-2 and VP alone treatment group respectively. In contrast to NA-2 and VP, TMZ did not have any significant effect on the expression of HIF-1α and VEGF, it is possible that increase in efficacy and growth inhibitory activity of TMZ in combination treatment group might also be related to the NA-2 and VP mediated down regulation of HIF- 1α and VEGF as both the markers have role in growth and proliferation also. Based on our observations, we conclude that NA-2, VP and TMZ can inhibit the growth of U87 glioblastoma cells by inducing apoptosis. NA-2 and VP may also inhibit proliferation by down-regulating HIF-1α and VEGF expression. Synergistic growth inhibitory activity of NA-2 and VP with TMZ may in part be related to their apoptotic and anti-proliferative activity. In short NA-2 and VP both have growthinhibitory activity alone which was further refined in combination with TMZ and they can be exploited for therapeutic purposes.