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Home > Development of Topical Hydrogel Membrances: Characterization and In-Vivo Evaluation

Development of Topical Hydrogel Membrances: Characterization and In-Vivo Evaluation

Thesis Info

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Author

Sarfaraz Ahmad

Program

PhD

Institute

The Islamia University of Bahawalpur

City

Bahawalpur

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Pharmaceutics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/14446/1/Sarfaraz%20Ahmad_Pharmaceutics_2019_IUB_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725892888

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Background and Objective In the pharmaceutical sciences, skin disorders and its topical treatment are gaining attention in advanced ways as compared to oral and traditional drug therapy. Topical targeted dug delivery with polymer gels as vehicle is to localized drug with enhanced therapeutic effect. As compared to controlled polymeric drug delivery, conventional oral and topical drug delivery has limitations of high dosage, more frequency of application and more irritation with non-compliance. Mupirocin (MP) is the better choice in skin wounds and in resistive bacterial infection when applied topically. The purpose of current study was to develop and optimize controlled release topical polymeric hydrogel membranes to deliver mupirocin locally with reduced dosage frequency. Different polymers, monomers with cross linkers in different proportions were used to formulate different types of membranes. To characterize and compare antimicrobial activity of mupirocin, In-vitro, ex-vivo and in-vivo evaluations were conducted for prolong wound and burn therapy with enhanced patient compliance. Methodology Modified free radical polymerization method was adopted for synthesis of different topical hydrogel membranes of mupirocin. Hydrogel membranes were characterized by in-vitro analysis and general characteristics, swelling studies to check the sensitivity with buffer solutions (pH 4.0, 5.5, 7.4), drug loading and in-vitro drug release studies. Structural, thermal and morphological analysis were conducted by fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning microscopy (DSC) and scanning electron microscopy (SEM). In-vitro permeation study, ex-vivo skin deposition study and skin irritation study were also conducted to measure and quantify the amount of drug permeated and deposited. Furthermore, quantification of mupirocin in skin samples of formulations were also performed after the revalidation of developed HPLC method. In-vivo study of selected formulation was conducted to measure bacterial count after application and compares the efficacy with marketed formulation. Results Results of FTIR, TGA, DSC, SEM, in-vitro release and permeation data confirmed the development of polymeric hydrogel membranes and formulation synthesized by low molecular weight chitosan polymer (LCS) was selected as optimized for in-vivo evaluation. Percent drug release of mupirocin for chitosan based membranes was 85.78% at pH 5.5 and 93.93% at pH 7.4. Permeation flux was 121.62μg/cm2.h-1 and deposited amount was up to 1224μg/1.5cm2 respectively. Developed and revalidated HPLC method was used for quantification of all formulations that deposited in rabbit skin. Irritation study data was confirmed that developed cross-linked polymeric hydrogel membranes were non-irritant to skin. In-vivo study of surgical wound model for hydrogel membrane with marketed formulation reveal that hydrogel and MF reduced the bacterial count after treatment to 4.27±6.3 and 4.31±7.9 log 10 CFU/wound and there was non-significant difference between hydrogel membrane applied once in a day and conventional MP ointment applied three times in a day (P>0.05). Conclusion It could be concluded that hydrogel membranes of low molecular weight chitosan could be classified as better topical membranes among the developed formulations that were highly effective to deliver model drug mupirocin for long term therapy of wound healing and other infectious skin disorders as they showed better in-vitro, ex-vivo and in-vivo results. Skin irritation study data also confirmed that developed formulations were safe and non-irritant to the skin.
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حدود کےنفاذ کی شرائط

حدود کےنفاذ کی شرائط
حدود کی تنفیذ کے لیے مندرجہ ذیل شرائط کا پورا ہونا ضروری ہے:
1۔ حدود کا اجراء ہر کسی کے لیے جائز نہیں بلکہ یہ حق صرف اسلامی حکومت کو حاصل ہے اور حکومت بھی اس وقت یہ سزا دے گی جب معاملہ کی پوری تحقیق ہو جائے اور ثبوت ، اقرار یا قرائن سے جرم ثابت ہو جائے اور کوئی شبہ باقی نہ رہے کیونکہ حد حق اللہ ہے اور شبہ سے حد ساقط ہوجاتی ہے۔ اسلامی قانون میں جائز نہیں کہ جرم ثابت ہو جانے کے بعد سزا میں کوتاہی کی جائے بلکہ ایسا کرنا جرم ہے۔ حد ودکا نفاذ اسلامی حکومت کے قیام سے ہو گااور امام کے ذمہ ہے ، جیسا کہ امام سرخسی ؒ نے لکھا ہے
"استيفاء الحد إلى الإمام"38 "حد کا استیفا ء امام کا کام ہے۔ "
حاکم وقت یا اس کا نمائندہ ہی حد کا نفاذ کر سکتا ہے ، جیسا کہ علامہ مرغینانی ؒ تحریر کرتے ہیں کہ
"حد قائم کرنے کے لیے ضروری ہے اس کو امیرالمومنین یا حاکم وقت یا حاکم کا نمائندہ قائم کرے۔ "39
2۔ آزاد ، عاقل ، بالغ اور مرضی سے فعل سر انجام دینے والے پر حد جاری ہوتی ہے۔ علامہ مرغینانی لکھتے ہیں جس پر حد لگائی جائے وہ "آزاد ، عاقل ، بالغ ہواور یہ فعل آزاد ی و مرضی سے ہوا ہو۔ "40
3۔ جس پر حد قائم کی جائے وہ سلیم البدن ہو۔ پاگل ، مجنون ، مریض، ناتواں، ضعیف اور نشہ کی حالت میں حد قائم نہ ہو گی۔ ہاں البتہ ان کمزوریوں کے دور ہونے پر حد قائم ہو گی۔ رسول اللہ ﷺ کے زمانے میں تندرست پر ہی حد جاری کی جاتی، سوائے رجم کے ۔ حضرت عبد الرحمان سے روایت ہے کہ نبی ﷺ نے حضرت علی...

The Issue of Human Cloning: A Review in Semitic Religions’ Context

Human cloning has emerged as a new and innovative technology in the reproductive and therapeutic science in the recent past. So far it has not been practiced over human beings but owing to its huge potential and possible scope, it has attracted the attention of not only the masses (particularly the infertile couples and LGBTQs etc) but the other stakeholders including the religious scholars from worlds’ prominent religions have given their views on this technology in order to guide their followers. This paper examines and reviews the religious points of view on human cloning. For this purpose, three Semitic religions in the world i-e Judaism, Christianity, and Islām have been examined. As far as Islam is concerned, this portion has been divided into two broad sections elaborating the Shī‘ah and Sunnī schools’ opinions. Being an innovative topic, the religious teachings do not address it directly hence the injunctions related to the reproduction are most relevant to it. Three Semitic religions have been examined from the perspective of admissibility or non-admissibility of human cloning, the rationale behind the verdict on human cloning and the possible solutions to the issues and problems faced by the followers in the case of acceptance or rejection of this biomedical technology. Most of the religions emphasize over adaptability of the natural mode of reproduction only, where male and female genders contribute to the reproductive cycle. The Semitic religions reject the reproductive cloning generally. The religious experts need to conduct more focused and updated research before coming to any conclusion about the permissibility or non-permissibility of this technique.

Induction of Resistance by Chemicals and Plant Extracts in Chickpea Against Ascochyta Rabiei

A series of experiments were conducted to determine the ability of different chemicals and plant extracts to induce resistance in chickpea plant against A. rabiei disease in the Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan. Firstly two hundred and seventy seven advanced lines/cultivars of chickpea obtained from various International and Indigenous Organizations were screened during the Rabi season 2005-06 for the source of resistance against chickpea blight disease by artificial inoculation of the germplasm with pycniosspore suspension of the pathogen. The screening revealed 02, 38, 39, 49 and 149 lines/cultivars to be highly resistant (immune), resistant, moderately resistant, susceptible and highly susceptible while in the next experiments 49 susceptible lines/cultivars were sown for the purpose of induction of resistance in high yielding lines/cultivars by comparing their yield potential under disease free and artificially inoculated The results revealed that the three chickpea cultivars C-44 with (575 kg h -1 ), Bittle-98 (543.75 kg h -1 ) and Pb-91 (533.50 kg h -1 ) gave highest yield under disease free as well as under artificially inoculated conditions with yield (144.6 kg h -1 ), (132.8 kg h -1 ) (111.2 kg h -1 ) of C-44, Bittle-98, and Pb-91 respectively. Upon induction of resistance by chemicals i.e. salicylic acid, Bion ® , KOH and plant extracts i.e. Azadirachta indica, Datura metel and Allium sativum revealed that significant disease reduction (79%) was provided by Bion ® in the cultivar C-44 at 1.2mM dose rate as compared to salicylic acid, however, the least was showed by KOH. Among the plant extracts the maximum disease reduction (43.5%) against the disease was observed by the application of Azadirachta indica leaf extract while Datura metel and Allium sativum did not prove effective in reducing the disease at the same time this resistance was remained persistent for 14 days. The analysis of mineral contents of induced un-inoculated and induced inoculated chickpea plants after seven and fourteen days revealed there were increased after the induction of resistance but this increase was more significant (P ≤ 0.05) upon inoculation with the pathogen after 14 th day time interval by the application of chemical but it was not significant in case of plant extract expect neem. Only Na content was decreased in Bion applied plant in the cultivar C-44 and Pb-98 further more Cu content was also decreased in salicylic acid, Bion and neem leaf extract treated plants in the cultivar C-44. The amino acids contents were also determined of both the induced un-inoculated and induced inoculated chickpea plants showed that the quantity of methionine, isolucine, tyrosine and phenylalanine content increased after induction and inoculation in three cultivars of chickpea and comparatively higher than induced un-inoculated plants. Increase in lysine content was more in C-44 upon inoculation in case of Bion and salicylic acid treatment but it decreased in Bittle-98. Arginine and aspartic acid contents also increased in all the cultivars expect in Bittle-98, however, aspartic acid content increased in case of garlic application. Variable response was also shown by threonine content by the application of chemicals and inoculation with pathogen in all the cultivars. Serine contents decreased in KOH, neem and datura extracts application in Bittle-98. Glutamic acid show variable response while proline decreased in C-44 and Bittle-98 after application of garlic extract. Glycine content decreased in Bittle-98 otherwise it increased in all the other cultivars. Garlic application showed decrease in alanine content in Pb-91 and Bittle-98.