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Development, Characterization and In-Vivo Evaluation of Emulgels Containing Avocado and Kiwi Fruit Extracts

Thesis Info

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Author

Rafique, Sadia

Program

PhD

Institute

The Islamia University of Bahawalpur

City

Bahawalpur

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Pharmaceutics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12716/1/Sadia%20Rafique_Pharmaceutics_2018_IUB_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725894742

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Nowadays, considerable attention has been given to those plants which have highest antioxidant value because they are used for the treatment of various dermal disorders such as acne, inflammation, hyper pigmentation, dry skin and for aging. They are also used as sunscreens, moisturizing and smoothing agents as synthetic agents have various adverse effects. The aim of the present study was to formulate and evaluate a thermodynamically stable carrier system which contains Persia americana and Actinidia deliciosa fruit extract and used as topical oil-in-water emulgels. Their characterization was done through in-vitro studies and was evaluated through in-vivo studies upon facial skin of healthy human female volunteers.Extracts of fruits i.e Persia americana and Actinidia deliciosa were prepared successively and the antioxidant activity of both fruits were determined by DPPH (2,2, diphenyl-1-picryl-hydrazyl) assay. The antioxidant activities of Persia americana and Actinidia deliciosa fruit extracts were 84% and 79% respectively. Total phenolic and flavonoid contents of both fruit extracts were determined by qualitatively and quantitatively. Suitable oil-in-water type of emulgels E1 containing 4% Persia americana fruit extract, E2 containing 5% Actinidia deliciosa fruit extract and E3 containing combination of 2% Persia americana and 2% Actinidia deliciosa fruit extracts and a base emulgel containing no fruit extract were prepared. All theses emulgel formulations were kept at four different stability storage conditions i.e at 8˚C, 25˚C, 40˚C and40˚C+ 75% RH for study duration of 3 months to observe the following physical parameters like colour, odour, thickness, pH, conductivity, centrifugation, rheological parameters and SEM (scanning electron microscopy). This study was performed according to the criteria of stability guidelines. In-vivo characterization of emulgel formulation was done by applying these formulations on facial skin of healthy human female volunteers for study duration of 3 months to evaluate the following parameters i.e skin melanin, erythema, moisture, sebum, elasticity, Surface evaluation of living skin (skin scaliness, smoothness, roughness and wrinkles) and R parameters of elasticity through cutometer. The study was single blinded and controlled with three groups of active formulation (E1, E2 and E3) and each group having 13 female volunteers. The statistical analysis was done through two- way ANOVA at 5% level of significance and also through paired sample t-test. Active formulations showed significant decrease in skin melanin contents, skin erythema contents, skin sebum contents while a significant increase in skin elasticity and skin moisture contents were observed with respect to base. Active emulgel formulation also showed a significant effect on SELS parameters in increasing the skin smoothness and a significant effect was also observed in decreasing the scaliness, roughness and wrinkleswith respect to base. Therefore it can be concluded from this study that active formulations (E1, E2 and E3) were found completely safe and effective to be used as anti-aging and skin whitening agent by improving the patient compliance.
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انکار بھی نہیں انھیں اقرار بھی نہیں

انکار بھی نہیں ، انھیں اقرار بھی نہیں
اتنا ہوا یہ دل کبھی لاچار بھی نہیں

دکھ درد ہم نے بانٹ لیے سارے شہر کے
آئی جو اپنی باری تو اک یار بھی نہیں

اس بخت کی یہ پستیاں بھی ہوں ملاحظہ
گل تو کجا ہے اپنے لیے خار بھی نہیں

اس طرح مفلسی کے ستائے ہوئے ہیں ہم
محرومیِ اناج ہے اور پیار بھی نہیں

جانے وہ کون لوگ تھے جن کو ملے ہیں یار
حاصل ازل سے ہم کو تو اغیار بھی نہیں

دولت خلوص کی ہے مرے پاس فہدؔ سب
اور وہ خلوص کا تو طلب گار بھی نہیں

أثر القرآن الكريم في الشعر الفلسطيني "محمود درويش" أنموذجاً

يشكل القرآن الكريم منبعا ثريا، يلجأ إليه الشعراء والأدباء في أعمالهم الأدبية المختلفة، لأنّه جزء ثابت في حياتهم، واستنادا على هذا وجد الشعراء ضالتهم في القرآن الكريم للتعبير عن خواطرهم مستلهمين منه معانيه، وقد ارتبط الشعر الفلسطيني خاصة بالقرآن الكريم باعتبار أن قضية احتلال فلسطين قضية دينية أكثر منها سياسية، إذا كيف تأثر الشعراء الفلسطينيين بما جاء من قصص وعبر في القرآن الكريم وكيف ترجموها في شعرهم لإشعال المقاومة والثورة؟ والهدف من هذه الدّراسة توضيح أنّ الشعر الفلسطيني ارتبط بالقرآن الكريم لاعتبار الشعراء الفلسطينيين قضية فلسطين قضية دينية ومن النتائج التي توصلنا إليها تأثر شعراء فلسطين بالقرآن الكريم في عدة جوانب منها استلهام المفردات القرآنية والرموز الدّينية والتّناص والإيقاع والموسيقى، وقد اعتمد الباحثان في الدّراسة على المنهج الوصفي التحليلي

Social History, Genetic Diversity and Origin of Date Palm in Baluchistan

PakistanstandsfifthamongleadingdateproducingnationsgloballywithprovinceofBaluchistan as the leading contributor. The long history of date palm cultivation in Baluchistan and its geo-political position in relation to Iran and Oman signifies its importance for diversity analysis. The present study was designed to examine the nature and level of genetic diversity in date palm collected from Baluchistan to explore its potential as a center of origin which is currently unclear. Fifty-two microsatellite markers were used to analyze the geneticrelationshipamonghundred(sixtyonefromBaluchistanandthirtyninefromPunjab) date palm cultivars. Different diversity indices were calculated for Baluchistan accessions and then compared them with Punjab genotypes. The results highlighted the presence of rich allelic diversity and high variation within the Baluchistan germplasm as compared to Punjab. The cluster analysis divided the entire population of hundred genotypes from both regions into seven groups with 50% admixed lines. The structure approach identified low levels of geographical structuring and suggested that mixed geographical distribution may be the result of gene flow between two regions. The variation detected through SSR markers highlighted that Baluchistan is rich in date palm diversity and it could be one of the regions claiming their position as centre of diversity especially in eastern gene pool. The estimated diversity could also be used for future breeding programs, association mapping, gene cloning and germplasm conservation. Punjab germplasm was also studied for morphologicalvariationforthirtytwoqualitativeandquantitativetraits. Theclusteringanalysis showed that the distribution of the cultivars into groups was independent of geographical origin. Angoor (Muzaffargarh) genotype showed good performance with regard to fruit width and petiole width. While Akhrot (Muzaffargarh) genotype had high statistic contributionof flesh taste. The resultssuggestedthat Muzaffargarhgermplasm was more diverse thanJhangandBahawalpur. Socialdataaboutcropdiversityandsocialimportancerevealed that growers used to cultivate different date palm cultivars on the basis of preferences and usage. Begum Jangi was the most preferred variety by all the respondents because of its high production potential and its longer storage time. The coefficients associated with the farming experience, income , education, age of trees and number of trees showed statistically significant and positive effect on the number of genotypes grown. While negative relation of education with number of genotypes was observed.