Search or add a thesis

Advanced Search (Beta)
Home > Diagnostic Potential of Indigenously Hyperproduced Urate Oxidase

Diagnostic Potential of Indigenously Hyperproduced Urate Oxidase

Thesis Info

Access Option

External Link

Author

Meraj, Munazzah

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2012

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/2165

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725900597

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The main purpose of this research work was to optimize the production of urate oxidase through mutagensis of Bacillus subtilis. The organism was subjected to ultra violet irradiation and chemical mutagenesis. Ethyle methane sulfonate treated B. subtilis (180 minutes) was proved to be the best for optimum production of urate oxidase by 3 log kill/survival curve. Fermentation medium was also optimized, it was found that substrate concentration (0.5%), fermentation period (36 h), pH (8.5), temperature (35 oC), yeast extract (0.3%) and sucrose (2%) enhanced the activity of the parent and mutant derived enzyme. The enzyme was purified by adopting different techniques i.e ammonium sulfate precipitation, ion exchange and gel filtration chromatography. It was observed that mutated enzyme exhibited 97.56 U/mg specific activity with 256.73 fold improvement. The purified urate oxidase was run on SDS-PAGE which determined a single band with molecular weight of 34 kDa. The purified BSM-2 possessed Km and V max value 0.067 M and 133.3 IU mg-1 min-1 respectively. The optimum pH and temperature for catalytic activity were 7.5 and 35oC respectively. The activation energy for formation of ES complex was 43.4 kJ/mol. Enthalpy (ΔH*), entropy of activation and Gibbs free energy demands for urate oxidase inactivation were 30.26 kJ/mol, -106.27 J mol-1 K-1, 62.99 kJ/mol respectively. Barium chloride, potassium cyanide and zinc sulfate decreased the activity of the enzyme. Whereas, sodium chloride (0.6M), potassium chloride (0.4M) and calcium chloride (0.4M) enhanced the enzymatic activity 123%, 117% and 119% respectively.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

نواب حاجی محمد اسحا ق خان بہادر

نواب حاجی محمد اسحاق خان بہادر

(مولانا عبدالسلام ندوی)

            نہایت افسوس ہے کہ علی گڑھ کالج کے آنریری سیکریٹری نواب حاجی محمد اسحاق خان بہادر نے پچھلے مہینے دل کی حرکت بند ہوجانے سے دفعتہ انتقال کیا، مرحوم مختلف خوبیوں کے بزرگ تھے اس لئے قوم میں مختلف حیثیتوں سے ان کا ماتم کیا جارہا ہے، ہم بحیثیت ایک علم دوست بزرگ کے ان کی موت پر آنسو بہاتے ہیں۔ (نومبر ۱۹۱۸ء)

 

قیام امن کے لئے حضرت عمر فاروق ؓکی خدمات

Peace has great importance both for the individual and the communal life. Wherever peace turned into unrest, the tendencies of social violence, mental sickness and insecurity start developing amongst the people. Peace and harmony were the hallmark of the reign of the 2nd caliph, Haḍrat ‘Umar. He gave the best governing mechanism to the people of Arab, when they were not fully aware of rules & regulation of government. Though the empire was wide spread, he exercised a great sort of command & control on it. He took the responsibility of providing his subjects their basic needs: Food, Shelter, Education, Peace and Justice. This was not only an ideal system of its time but became the role model for the modern welfare state. Peace and harmony is as important for a state as food & air are for life. Allāh has strongly emphasized in The Holy Qur’ān" on two things i. E., "Disharmony & hunger" which should be eliminated from a society. Haḍrat ‘Umer during his reign of 10 years presented Islām as a religion of peace & harmony, a religion, which respects humanity, peacefully resolves disagreements and curtails misuse of power. He himself possessed the qualities of peace & harmony to an utmost level, which were the traits of our Holy Prophet’s (ﷺ) personality. It is important to follow the Khilāphah of Haḍrat ‘Umar to bring peace & justice in the society.

Impact of Religious Affiliations on Rational Thinking of Secondary School Students in Pakistan

Individuals‟ thinking styles play central role in their decision making process. Epstein, Pacini, Denes-Raj, and Heier, (1996) and Pacini and Epstein (1999) described two major types of thinking patterns i.e. rational and experiential. Prior research investigated different factors that affect thinking styles of people. These factors range from inherited instincts to environmental pressures. The current study aimed to examine the impact of religious affiliations of secondary school students on their rational thinking. Profound review of related literature leaded the researcher to adapt age-universal I-E scale developed by Allport and Ross (1967) and revised by Kirkpatrick, Moberg, and Lynn (1988) and rational experiential inventory for adolescents (REI-A) developed by Pacini and Epstein (1999) and revised by Marks, Hine, Blore, and Phillips (2008). The age universal I-E scale measures three dimensions of religiousness i.e. intrinsic, extrinsic personal and extrinsic social religiosity of the individuals of all ages. The REI-A is to scale rational and experiential thinking of adolescents. The 3517 secondary school students having religious affiliations with Hinduism (1050), Christianity (1073) and Islam (1394) were included in the sample using non-random sampling techniques. It was ensured that only the students studying government prepared syllabus were selected in the samples. The adapted instruments were translated into Urdu and validated through the opinions of ten field experts. All five subscales demonstrated high reliability of the data of Pakistani samples. Collected data were entered in SPSS files. The negative statements were recoded and results were presented in graphs and tables. Results of skewness, Kurtosis, Kaiser-Meyer-Olkin (KMO) measure of sampling adequacy and Bartlett‟s test of sphericity supported the normality of sample distribution and homogeneity of data. Principal component analysis, rotated component matrix and structural equation models endorsed the structures of scales and subscales. Statistical analysis of the data was done using t test, ANOVA, correlation coefficients and regression analysis. Findings revealed that Muslim secondary school students were comparatively more intrinsically religious and rational in thinking than their Hindu and Christian fellows. In contrast the Christian students were comparatively more extrinsic personal and extrinsic social religious than their Hindu and Muslim secondary school fellows. Similarly, the Christian students were more experiential in thinking than their fellows. Major contribution of xvi the study was to trace out the relationship between religious orientations and thinking patterns of the students. It was concluded that rational thinking of Hindu, Christian and Muslim secondary school students (separately and collectively) was directly associated with their intrinsic and extrinsic personal religiousness and indirectly correlated with their extrinsic social religiosity. The three aspects of religious orientations moderately explained the variances in rational thinking of the Hindu and Muslim secondary school students whereas for the Christian students this share was significantly small. The study proposed further research in the field to investigate the factors (social, cultural, political, financial etc.) that played role in forming religious orientations of the students having different faith. The study also suggests further investigation of factors that transform students thinking styles with the passage of time.