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Disease Predictive Model for the Management of Bemisia Tabaci Genn. Population and Tomato Leaf Curl Virus Disease Incidence

Thesis Info

Access Option

External Link

Author

Zeshan, Muhammad Ahmad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7802/1/Muhammad_Ahmad_Zeeshan_Plantpthology_2015_UAF.pdf

Added

2021-02-17 19:49:13

Modified

2023-02-17 21:08:06

ARI ID

1676725910135

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106. Al-Quraysh/The (Tribe of) Quraysh

106. Al-Quraysh/The (Tribe of) Quraysh

I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

106:01
a. In gratitude for safeguarding the Tribe of Quraysh,

106:02
a. and for their security of annual trade journey of winter to Yemen and of summer to Palestine-Syria, on which their prosperity depended,

106:03
a. they should submit in awe and worship to Rabb - The Lord of this House of Ka’bah,

106:04
a. for HE is The One WHO provides them with food against impoverishment, and
b. gives them peace and security against fear/danger.

NILAI-NILAI PENDIDIKAN ISLAM DALAM TRADISI MERTI DESO DI DESA SUKU MENANTI REJANG LEBONG, BENGKULU

This study aimed at finding out Islamic education values embedded in the tradition of Merti Deso in Suku Menanti Village, Sindang Jati District, Rejang Lebong Regency, Bengkulu. This study used a descriptive qualitative approach. The data collection techniques used in this study encompassed observation and interviews conducted periodically. After the data were collected, the data were further analyzed by means of Miles, Haberman, and Saldana’s version of stages, namely data selection, data presentation, and drawing conclusion. This study concluded that the Islamic education values embedded in the tradition of Merti Deso were religious and spiritual values, muamalah/social values, and economic values.    

Transgenic Expression of Insecticidal Toxins under Different Promoters for the Control of Insect Pests.

Insect pests are the major reasons for low yield in agricultural crops. Crop losses due to insect pests can be as high as 25 %, depends upon the climatic conditions and other factors; several strategies have been adopted for the control of insect pests. Recently developed biotechnological applications, several transgenic plants have been developed for insect resistance. Bacillus thuringiensis is one of the most successful examples of insecticidal toxins being used for insect control (especially bollworms). Sucking pests are also major cause of reduced yield in agricultural crops. Sucking insects suck the cell sap from the phloem tissues of plants and also act as a vector for virus transmission. Recent trends in agriculture towards reducing pesticide use and bringing ecological sustainability have led to increased interest in spiders as potential biological control agents. Spider venoms are complex cocktails of toxins that have evolved specifically to kill insects. Spider toxin (Hvt) gene from the Australian funnel web spider which is a calcium channel antagonist has been expressed in tobacco plants to develop resistance against some major insect pests (Bollworms). In the present PhD research work, the main focus was to develop resistance against major insect pests; American bollworm (Heliothis armigera) and mealybug (Phenococcus solenopsis). Different gene constructs were prepared and transformed in tobacco plants (Nicotiana tabacum) through Agrobacterium mediated plant transformation. Spider toxin (Hvt) gene was cloned under phloem specific RSs1 and RolC promoters and lectin gene from onion Allium cepa L agglutinin (ACA) and kallar grass Leptochloa fusca (LfL) was cloned under 2X35S promoter. PCR, Southern hybridization and real time qPCR analysis showed successful transformation and expression of insecticidal toxin genes in N. tabacum. Insect bioassays of transgenic plants were carried out in the laboratory and glasshouse conditions. Results showed 93.75 and 100 % mortality of H. armigera larvae when fed on detached leaves from transgenic tobacco plants expressing spider toxin (Hvt) gene under RolC and RSs1 promoters within 72 hour respectively. H. armigera larvae released on the detached leaves of transgenic plants expressing lectin gene from kallar grass and onion under 2X35S promoter showed, as 75 and 81.25 % mortality within 72 hours respectively. No mortality of H. armigera larvae was observed on the detached leaves of non-transformed tobacco plants up to 72 hours. Transgenic plants expressing spider toxin under RSs1 and RolC promoters gave good resistance against P. solenopsis on detached leaves; 68.75 and 75 % and on live transgenic tobacco plants 70 and 62.5 %, up to 10 days respectively. Transgenic plants expressing lectin gene from onion and kallar grass under 2X35S promoter showed high level resistance against nymphs of P. solenopsis on detached leaves; 87.5 and 81.25 % and on live transgenic plants; 90 and 87.5 % mortality of P. solenopsis within 10 days respectively. No mortality of P. solenopsis was recorded on non-transformed (control) tobacco plants up to 10 days. This study described expression of Hvt gene under RSs1 and RolC promoters and lectin gene under 2X35S promoter. Both genes are helpful for controlling chewing insects like H. armigera larvae and sap-sucking insect like P. solenopsis. This study has a potential for developing insect resistant transgenic crops. These crops can reduce the use of harmful pesticides, fuel use, input cost and yield losses, while increase profitability and enhance biodiversity and save the environment.