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Home > Diversity, Recombination and Replication in Cotton Leaf Curl Geminiviruses

Diversity, Recombination and Replication in Cotton Leaf Curl Geminiviruses

Thesis Info

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Author

Saleem, Huma

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Plant Breeding & Genetics

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9630/1/Huma_Saleem_PBG_UAF_2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725919429

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Several viruses infect cotton plant across the globe. Due to climate change, which favors the insect’s growth and reproduction, the diseases transmitted by insect vectors are increasing at alarming rate. The viruses causing leaf curl disease are common in Africa and Asia. Cotton crop is infected by single stranded DNA viruses (Family, Geminiviridae) in Pakistan. It was originally limited to Pakistan, but now it has spread to India, China and Philippines. In the New World a virus causing leaf crumpling is known to infect cotton. The studies in this thesis were aimed at a clearer understanding of the virus species responsible for the CLCuD complex and possible reasons it became an epidemic. These single stranded DNA viruses were surveyed, isolated and amplified with a novel one step PCR protocol to identify the major three viruses, namely cotton leaf curl Multan virus, cotton leaf curl Kokhran virus and Burewala strain of Kokhran virus. Furthermore, PCR and RCA was used to quickly cloned and sequenced full-length viruses and satellites from cotton and alternate host tomato plants to estimate the extent of diversity of begomoviruses in Punjab. The sequence analysis revealed that viruses co-circulating in cotton and tomato plants but may only create diseases in one host. Furthermore, these viruses are harboring several distinct satellites. The mutation and recombination analysis revealed that cotton leaf curl geminiviruses (just like RNA viruses) have a high and specific pattern of recombination and mutation and favored a combination of viral molecules, which have the capacity to spread at higher rate compared to other strains of cotton leaf curl geminiviruses. In this manuscript, the diversity and spread of cotton leaf curl disease is discussed in depth to understand the viral evolution in Pakistan, uses for host plant resistance, and implicated for research and management strategies to prevent new epidemics worldwide.
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ماحول کی آلودگی

ماحول کی آلودگی
اللہ تعالیٰ قادرِ مطلق ہے، ہرشخص کے رزق کی ذمہ داری اس نے خود لے رکھی ہے، اس نے اس کے بیٹھنے،اُ ٹھنے، چلنے پھرنے کے کچھ قاعدوں اور کلیوں کا قرآن و حدیث کی شکل میں تعین کر دیا ہے، اور اسلام جو اُس وحدہٗ لاشریک کے نزدیک پسندیدہ دین ہے، گو انسان کے لیے مکمل ضابطہ قرار دیا ہے۔ جہاں دیگر مذاہب میں ماحول کو صاف ستھرا رکھنے پر زور دیا گیا ہے، وہاں اسلام میں بھی اس کی تاکید کی گئی ہے۔ جب آلود ہ، آلودگی، آلائش قسم کے الفاظ نظروں کے سامنے متحرک یاساکت صورت میں ہوتے ہیں تو بغیر کسی ظاہری تعفّن کے بغیر کسی نظر آنے والی عفونت کے دماغ کے جملہ گوشوں کومتعفن کر دیتے ہیں۔ جب یہ غیر مرئی تصورات ،خیالات ، دماغی آلودگی کا سبب بنتے ہیں، تو مادی شکل میں موجود آلودگی کا وجودکتنا قابل نفرت ہوگا۔
آلودگی کے تصور کو ہم نے محدود کر دیا ہے کوئی چیز اگر اپنی اصلی اورحقیقی شکل میں موجود نہ ہو تو وہ آلودگی کی آمیزش کا شکار ہے۔ اُس میں آلائش شامل ہے وہ نہ صرف منحوس وجود سے اپنے آپ کو نقصان پہنچاتی ہے بلکہ اس کی زد میں آنے والی گردو پیش کی اشیاء بھی متاثر ہوتی ہیں۔ اللہ تعالیٰ نے انسان کو اشرف المخلوقات بنایا اور اُس کو شرف، عظمت، بزرگی کے زیور سے آراستہ اور مزیّن فر مایا، اور اس کو اپنی صورت پر پیدا فرمایا۔ گلستان ہستی میں چلنے والی بادِنسیم ، در یائوں، سمندروں، بحروں اور بحیروں کی لہروں سے پیدا ہونے والی کھڑ کھڑاہٹ ، صحراؤں کی سنسناہٹ ، فضاؤں کی سرسراہٹ، سورج کی چمک، ستاروں کی دمک، چاند کی چاندنی ، تو حید کی مئے سے مست ہونے والے کی راگنی ، نرگس و گلاب کی پتیوں پر...

From the Chief Editor's Desk

Medical journals are a credible source of disseminating research and innovations, and Launching a medical journal is a challenging task. Many medical science journals are establishing a platform to publish quality research but still the task is tough and requires perseverance and hard work. Shalamar Medical and Dental College (SMDC) Lahore, strives to promote a culture of research. As part of this initiative, SMDC had launched ‘Medical Journal of Sakina Begum Institute’, but publication of the second volume was delayed due to the pandemic. Moreover, the name of the journal had to be changed due to some administrative issues. However, the committed editorial team was successful in bringing efforts for the latest issue to fruition.

An Investigation into the Influence of Different Welding Methods on the Microstructure, Mechanical Properties and Residual Stress Distribution in Ti-5Al-2. 5 Sn Alloy

The use of welding processes, especially for joining of aerospace alloys has gained a significant importance in the recent years. This is owing to the enhanced joint efficiency, increased sealing ability and reduced weight of the welded structures as compared to riveted structures. Moreover, the modern trend in aerospace industry has shifted towards the use of titanium alloys, due to their high strength to weight ratio and good corrosion resistance. This work is focused on the welding of the well-known α titanium alloy Ti-5Al-2.5Sn, which haslow cost alloying elements as compared to the mostly widely used Ti-6Al-4V alloy, has a good weldability and is also more suitable for high temperature aerospace applications. Tungsten inert gas (TIG), laser beam welding (LBW) and electron beam welding (EBW) are the mostly used welding methods for titanium alloys. As compared to TIG welding, LBW and EBW are always the preferred welding methods due to low heat input and deep penetration characteristics. However, TIG welding is mostly employed industrially due to significantly less capital cost and ease of automation due to reduced equipment size. A number of gaps were identified in the open literature related to the welding of Ti-5Al-2.5Sn alloy. Firstly, few studies are available in the public domain related specifically to the welding of Ti-5Al-2.5Sn alloy using TIG, LBW and EBW. Moreover, the reported work related to comparison of TIG, LBW and EBW of other titanium alloys is limited and there is a need of in-depth, comprehensive comparison of these welding processes in terms of microstructure, mechanical properties and residual stresses in the welded structures. The opportunities available for parametric analysis of LBW process in titanium alloys and optimization of the pulsed TIG welding process for titanium alloys especially Ti-5Al-2.5Sn alloy have not been explored to full potential. The present work aims mainly at improving the pulsed TIG (P-TIG) welding process for 1.6 mm thick Ti-5Al-2.5Sn alloy sheet so that resultant microstructure, mechanical properties and residual stresses are comparable to that of pulsed LBW (P-LBW) and EBW weldments. Microstructure, oxide contents and microhardness of fusion zone, HAZ width, weld zone strength, tensile residual stresses and plate deformations were measured to compare the performance of the weldments. P-LBW was found to be most suitable in terms of these performance attributes of TI-5Al-2.5Sn welds due to low heat input which led to a complete martensitic transformation in the FZ. The absence of shielding gas due to vacuum environment in EBW was beneficial in terms of increasing the joint quality (low oxide contents). However, an increased width of heat affected zone (HAZ) and partial α’ martensitic transformation in FZ of EBW was observed as compared to P-LBW. High heat input and much wide heat source in P-TIG led to coarse microstructure and partial martensitic transformation in FZ resulting in increase of FZ and HAZ width, plate deformations and tensile residual stresses and a reduction in FZ microhardness and weld zone strength. The optimization of P-TIG welding was performed using Box-Behnken design of experiments in which a mathematical was developed to establish the relation between the welding input factors (peak current, background current and welding speed) and output responses (FZ width, HAZ width, FZ grain size, ultimate tensile strength, notch tensile and impact strength, and elongation, longitudinal and transverse residual stresses). The dependence of output responses on the inputs of P-TIG welding and its physical significance in the context of microstructure was discussed in detail. Optimization was performed through different criteria and a multi-response optimization was suggested to maximize the joint strength, impact properties and minimize the residual stresses. Results were experimentally validated and the range of welding input parameters were recommended through overlay plots for industrial application.