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Dna Barcoding of the Ethnobotanically Important Plants of District Dera Ghazi Khan, Punjab

Thesis Info

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Author

Saadullah

Program

PhD

Institute

Government College University Lahore

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8189/1/Saad_Ullah_Botany_HSR_2017_GCU_Lahore_23.11.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725920191

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In the present research work, 665 plant specimens belonging to 247 species, 62 families of 27 different orders in 5 major classes of plant kingdom i.e. Mangolopsida (Dicot), Lilipopsida (Mononcot), Pinidae (Gymnosperms), Pteropsida and Bryopsida were collected from different parts of district Dera Ghazi Khan, Punjab, Pakistan. Herbarium sheets with mounted and identified specimens of all the species were deposited in Dr Sultan Ahmad herbarium, GC University, Lahore after posting voucher numbers. The ethnobotanical knowledge on the use of local plants was collected from four hundred pertinent people belonging to all walks of life through their interviews using a semi-structured questionnaire. The plants were found in use by locals as food, feed, medicine, source of fuel and furniture wood, etc. The leaves of the plants were found most frequent in use for treating various ailments, as compared to whole plant or other parts of the plant. Tissue sampling and DNA barcode sequencing was carried out at NIBGE, Faisalabad and Canadian Centre of DNA Barcoding (CCDB), University of Guelph, Canada, respectively. At Family level, 61/62 (98.39%) families were found having at least one sequence of rbcl while no matK sequence in 6/27 (22.22%) orders and 13/62 (19.67%) families. But 49/61(80.33%) families were found to have both rbcl and matK sequences. A total of 1015 barcodes were sequenced, with 630/665 (94.73%) barcodes having rbcl sequences of 235/247 (95.14%) species representing 176 genera and 62 families while 385/665 (57.90%) matK sequences of 185/247 (74.90%) species from 146 genera and 49 families. The data analysis was accomplished on the basis of three criteria; PCR Amplification and Sequencing Success of rbcl and matK; Barcode Gap Analysis (Nearest Neighbor (NN) Analysis) and Neighbor Joining (N/J) Cluster Analysis. PCR and sequencing success were found very high for the rbcl region, i.e. 94.73% (630 / 665 specimens) while matK had the lowest overall rate of recovery, i.e. 57.9% (385 / 665 specimens). The matK showed the highest sequence quality for almost all the recovered samples. Barcode Gap Analysis revealed that 95.22% sequences of matK and 96.63 % of rbcl exhibited no intra-specific variation. The inter-specific divergence varied from 0.0% to 19.06% for rbcl while 0.0% to 4.12% for matK. Out of 105 congeneric species with rbcl sequences, 69/105 (65.71%) were identified while in congeneric species with matK sequences 54/71 species (73.24%) were successfully differentiated. In pair wise divergence across all the species (non-congeneric), both xiv matK and rbcl sequences showed clear boundaries between the 84.21% (96/114) and 70.45% (93/133) of the species, respectively. In Barcode Gap Analysis, matK showed more discriminatory power than rbcl. Among taxa, patterns of sequence divergence was visualized by means of Neighbor-Joining (NJ) cluster on MEGA6. In all three trees of rbcl, matK and rbcl+matK, assignment of families within their respective orders was 83.61% (51/61), 79.59% (39/49) and 83.67% (41/49), respectively. In all three trees of rbcl, matK and rbcl+matK, species were identified on the basis of “Bootstrap Threshold Value” and “Monophyly”. In neighbor trees (N/J) of rbcl, matK and rbcl + matK, the number of monophyletic families were 49/61(80.33%), 40/49 (81.63%) and 40/47 (85.11%), respectively. In neighbor joining (N/J) trees of rbcl, matK and rbcl + matK, the number of monophyletic families were 49/61(80.33%), 40/49 (81.63%) and 40/47 (85.11%), respectively. Therefore, on the basis of monophyletic recovery and node support, all three neighbor joining trees were found best resolved monophyletic trees having more than 80% monophyletic families.
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ڈاکٹر علامہ محمد اقبالؒ

ڈاکٹر علامہ محمد اقبالؒ (۱۸۷۷ء ۔ ۱۹۳۸ء) سیالکوٹ کے محلہ چوڑی گراں میں پیدا ہوئے۔ ’’اسرارِ خودی‘‘ علامہ کی پہلی شعری تصنیف ہے جو ۱۲ ستمبر ۱۹۱۵ء میں شائع ہوئی۔ یہ کتاب فارسی زبان میں فلسفہ خودی کے موضوع پر لکھی گئی ہے۔ کیمبرج یونیورسٹی کے پروفیسر ڈاکٹر نکلسن نے اس کا انگریزی زبان میں ترجمہ کیا۔ دوسری کتاب رموز بے خودی ۱۰ اپریل ۱۹۱۸ء میں شائع ہوئی۔ یہ کتاب اسرارِ خودی ہی کی توسیع تھی اور تسلسلِ خیال۔ علامہ نے اسے اسرارِ خودی خودی کا حصہ دوم قرار دیا۔(۱۹۹) ’’پیامِ مشرق‘‘ علامہ اقبال کی تیسری تصنیف ہے۔ یہ شعری مجموعہ فارسی زبان میں ہے جو ۹ مئی ۱۹۲۳ء میں شائع ہوا۔ جرمنی کے شہرت یافتہ مستشرق ڈاکٹر ہانسی مائنکے نے اس شعری مجموعے کا جرمنی زبان میں ترجمہ کیا۔ اقبال کا چوتھا شعری مجموعہ ’’بانگ درا‘‘ اردو زبان میں ۳ ستمبر ۱۹۲۴ء میں شائع ہوا۔ ’’بانگِ درا‘‘ میں اقبال کا ابتدائی اردو کلام ہے۔ پہلے حصے میں ابتداء سے ۱۹۰۵ء تک کا کلام اور دوسرے حصے میں ۱۹۰۵ء سے ۱۹۰۸ء تک کا کلام ہے۔ ’’زبورِ عجم‘‘ اقبال کا پانچواں شعری مجموعہ ہے جو جون ۱۹۲۷ء میں شائع ہوا۔ یہ مجموعہ فارسی زبان میں ہے۔ ’’جاوید نامہ‘‘ اقبال کا چھٹا فارسی شعری مجموعہ ہے جو فروری ۱۹۳۲ء میں شائع ہوا۔ ’’مسافر‘‘ (مثنوی) کا آغاز اقبال کے سفرِ افغانستان سے واپسی پر ہوا۔ اس کی اشاعت ۱۹۳۴ء میں ہوئی۔ ’’بالِ جبریل‘‘ اقبال کا ساتواں اردو شعری مجموعہ ہے جو جنوری ۱۹۳۵ء میں شائع ہوا۔ یہ مجموعہ غزلیات اور مختلف عنوانات پر نظموں پر مشتمل ہے۔ اقبال کا آٹھواں شعری مجموعہ ’’ضربِ کلیم‘‘ جولائی ۱۹۳۲ء میں شائع ہوا۔ یہ مجموعہ مختلف عنوانات پر نظموں پر مشتمل ہے۔

نواں مجموعہ مثنوی ’’پس چہ باید کرداے اقوامِ مشرق‘‘ ہے جس کی اشاعت اکتوبر ۱۹۳۲ء کو ہوئی۔ ’’ارمغان حجاز‘‘ اقبال کا دسواں شعری مجموعہ جو فارسی اور اردو کلام پر مشتمل ہے۔ اس کی اشاعت نومبر ۱۹۳۸ء میں ہوئی۔

اقبال نے اپنی...

Devasahayam: The First Martyr For Jesus Christ In Travancore

Travancore was the first and foremost among the princely states of India to receive the message of Jesus Christ. According to tradition, St. Thomas the Apostle came to India in 52 A.D. He made many conversions along the west coast of India. It had to the beginning of the Christian Community in India from the early Christian era. He attained martyrdom in 72 A.D. At Calamina in St. Thomas mount, Madras. He was the first to be sacrificed for the sake of Christ in India. During the close of the second century A.D. The Gospel reached the people of southern most part of India, Travancore. Emperor Constantine deputed Theophilus to India in 354 A.D. To preach the Gospel. During this time the persecution of Christians in Persia seemed to have brought many Christian refugees to Malabar coast and after their arrival it strengthened the Christian community there. During the 4th century A.D. Thomas of Cana, a merchant from West Asia came to Malabar and converted many people. During the 6th century A.D. Theodore, a monk, visited India and reported the existence of a church and a few Christian groups at Mylapore and the monastery of St. Thomas in India. Joannes De Maringoly, Papal Legate who visited Malabar in 1348 has given evidence of the existence of a Latin Church at Quilon. Hosten noted many settlements from Karachi to Cape Comorin and from Cape Comorin to Mylapore. The Portuguese were the first European power to establish their power in India. Under the Portuguese, Christians experienced several changes in their general life and religion. Vas-co-da-gama reached Calicut on May 17, 1498. His arrival marked a new epoch in the history of Christianity in India. Many Syrian Catholics were brought into the Roman Catholic fold and made India, the most Catholic country in the East. Between 1535 to 1537 a group of Paravas were converted to Christianity by the Portuguese. In 1544 a group of fishermen were converted to Christian religion. St. Francis Xavier came to India in the year 1542. He is known as the second Apostle of India. He laid the foundation of Latin Christianity in Travancore. He could make many conversions. He is said to have baptized 30,000 people in South India. Roman Congregation of the propagation of Faith formed a Nemom Mission in 1622. The conversion of the Nairs was given much priority. As a result, several Nairs followed Christian faith particularly around Nemom about 8 k.m. South of Trivandrum. Ettuvitu pillaimars, the feudal chiefs began to persecute the Christians of the Nemom Mission. Martyr Devasahayam, belonged to the Nair community and was executed during the reign of Marthandavarma (1729-1758). It is an important chapter in the History of Christianity in South India in general, and of Travancore in particular.

Sustainable Management of Spotted Bollworms, Earias Vittella Fabricius and Earias Insulana Boisduval , Lepidoptera: Noctuidae on Okra, Abelmoschus Esculentus L. in Punjab, Pakistan

The studies were conducted to determine the resistant/susceptible responses based on fruit and shoot infestation (%) in various okra genotypes against Earias vittella (Fab.) and E. insulana (Boisd.) under field conditions during 2006 and 2007. From the preliminary screening trial during 2006, among 30 genotypes of okra Parbani kranti (18.93%), Pusa sawani (17.89%), and Ikra-1 (17.32%) appeared as susceptible while Diksha (8.17%) and Sabz pari (8.36%) appeared as resistant based on fruit infestation were selected for final screening trial during 2007. Almost similar results were observed regarding shoot infestation caused by Earias spp. in different genotypes of okra during 2006. Maximum fruit infestation during 2007 was 19.73 followed by 18.49 percent on Parbani kranti and minimum (10.46 percent) was observed on Diksha, whereas maximum shoot infestation was on Parbani kranti (35.43) and minimum was found on Diksha (12.74 percent). On an average basis of both years’ studies, the maximum HPSI based on fruit infestation was recorded to be 16 percent each on Parbani kranti and the genotype Diksha was proved to be comparatively resistant with minimum HPSI i.e., 7 percent. The genotype Diksha were again found to be resistant based on shoot infestation with minimum HPSI i.e. 6 percent while maximum HPSI (15 percent) was observed each in Parbani kranti, Pusa sawai and Ikra-1, (15 %) and appeared as comparatively resistant and susceptible genotypes, respectively. All the weather factors showed significant correlation with the fruit infestation during both year studies as well as on average basis except minimum temperature during 2006 which had non significant effect on the fruit infestation fluctuation. Maximum temperature, minimum temperature and average temperatures showed positive effect whereas relative humidity and rainfall exerted negative correlation on the fruit infestation during 2006 and 2007 separately as well as on cumulative basis. Multiple Linear Regression Models revealed that maximum temperature was the most important factor which contributed maximum for fruit and shoot infestation i.e. 60.50 and 53.20% for average of both the study years, respectively. The impact of all the factors on an average of both the study years when computed together was found to be 67.00 and 55.50%, for fruit and shoot infestation, respectively. Amongst various physio-morphic plant characters, ten fruit weight, fruit yield per plant and fruit length showed negative and significant correlation with the fruit infestationcaused by Earias spp. on okra, whereas hair density on midrib, hair length on vein, hair density on lamina and thickness of leaf lamina exerted positive and significant correlation on the fruit infestation. Plant height, number of primary branches, leaf moisture, hair length on midrib, hair density on vein, hair length on lamina, hair density and length on fruits, leaf area and fruit weight showed non significant correlation with the fruit infestation. Amongst chemical plant characters, crude protein, nitrogen, total lipids, reducing sugars, non reducing sugars, cellulose, lignin, NDF, total minerals, potassium, phosphorus, magnesium and copper had significant and positive correlation with the fruit infestation whereas manganese and zinc contents in the fruits of okra showed negative and significant correlation with the fruit infestation. Hemi cellulose and ADF showed non significant correlation with the fruit infestation. Multivariate linear regression models revealed that hair density on midrib was the most important physical character which showed significantly negative impact and contributed 42.7 % role in the fluctuation of fruit infestation followed by ten fruit weight, fruit yield per plant, hair density on lamina, hair length on vein and hair length on lamina with 31.4, 15.5, 0.8, 0.5, and 0.1 % contribution, respectively. The 100 R 2 values were observed to be 91.0 when the effect of all the physio-morphic plant characters analyzed together. Crude protein was the most important character which showed positive and significant impact in the fluctuation of fruit infestation and contributed 79.9% role followed by reducing sugars with 12.6 % role in fluctuation of fruit infestation caused by Earias spp. on okra. Various practices viz. release of Trichogramma, weeding/hoeing, neem extract, hand picking and spray of Tracer for the control of Earias spp. were applied singly and in their possible combinations in the research areas of PARS, UAF and ERI, Faisalabad during 2007 on a resistant genotype of Okra (Diksha). All the treatments showed significant difference with one another on fruit and shoot infestation caused by Earias spp. on okra. Hand picking and spray of Tracer was found to be the most effective resulted in minimum fruit infestation i.e. 7.87% followed by the spray of Tracer only with 8.37% fruit infestation as against 25.46% in control treatment. Spray of Tracer showed minimum shoot infestation i.e. 11.26% followed by T11 (hand picking + spray of Tracer) with 12.52% shoot infestation. Application of hand picking + neem extract and Tracer resulted in maximum increase in yield over control i.e. 56.61 kg/ha and found to be the most benefited to the farmers with actual CBR of 1:20.28.