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Home > Dna Based Identification of Aphid Species and Vector-Virus Association Analysis of Aphid Borne Luteovirus

Dna Based Identification of Aphid Species and Vector-Virus Association Analysis of Aphid Borne Luteovirus

Thesis Info

Access Option

External Link

Author

Naseem, Muhammad Tayyib.

Program

PhD

Institute

Pakistan Institute of Engineering and Applied Sciences

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9451/1/Complete%20thesis%20file%20final%20for%20PIEAS.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725920634

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Aphids (Hemiptera: Aphididae) are globally important crop pests and vectors of viral disease, but their identification is generally difficult because of their small size and inconspicuous morphological differences. Sequence variation in the mitochondrial cytochrome c oxidase I (COI-5ʹ) gene (DNA barcode) has proven effective for the identification of species in many groups of insect pests and also in aphids. In this research, 728 aphids were collected from various plant hosts in north-central Pakistan, and identified and analyzed at COI barcode. Three species (Periphyllus lyropictus, Aphis nasturtii and Aphis astragalina) were reported for the first time in Pakistan. The Barcode Index Number (BIN) system assigned the 728 sequences to 52 BINs, while the Automatic Barcode Gap Discovery detected 51 partitions, a count that coincided with the number of monophyletic lineages recognized by Neighbor-Joining analysis and Bayesian inference. Congeneric species showed an average of 7.26% sequence divergence (range = 0.2-10.32%) at COI, while intraspecific distances averaged 0.13 (ranged from 0-3.19%). BIN analysis suggested that specimens with intraspecific divergence >3.0% actually involved a species complex. In fact, sequences for three major pest species (Aphis gossypii, Sitobion avenae and Aphis craccivora) showed deep intraspecific divergences, providing evidence that each was a cryptic species complex. The study compiled the first barcode reference library for the aphids of Pakistan, and examined global haplotype diversity in six important Luteovirus vector aphid species. This study also examined the aphid-Luteovirus association considering the carrot mottle disease (CMD) which is caused by co-infection of Carrot red leaf virus (CtRLV), Carrot mottle virus (CMoV) and Carrot red leaf virus RNA (CtRLVaRNA), and transmitted by aphid, Cavariella aegopodii in a persistent, non-propagative manner. Myzus persicae is an important vector of many plant viruses but its involvement in the spread of CMD has not been documented. The current study determined the involvement of M. persicae in the transmission of virus complex, responsible for CMD, by transmission studies. M. persicae were exposed to the CMD-infected plants of chervil and then transferred to disease-free plants (chervil, fennel, celery, carrot, cilantro and parsley) of the family Apiaceae. Healthy plants infested with the xiii viruliferous M. persicae developed typical CMD symptoms. The transmission of respective viruses was confirmed through RT-PCR by amplifying the target genes with CtRLV-, CMoV- and CtRLVaRNA-specific primers. Sequence comparisons of the amplified virus genes with those in the GenBank showed high sequence diversity in the three components of the disease complex causing CMD both in amino acids and nucleotides.
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المبحث الخامس: الشوق للقاء الحبيب

المبحث الخامس: الشوق للقاء الحبيب

قصيدة "أشواق و أحزان" لنازك الملائكة([1])

أین منّي حرارۃُ الأمسِ ، والحا

ضرّ یمشي بین الأسی والخمود ؟

آسفاً للماضي الإلھيّ ھل ما

تت أغان في فؤادي الوحید ؟

آہِ یا شاعري لماذا تھاوی

تِ بعیداً وراءَ أمسي البعیدِ ؟

وأنا لم أزل صلاۃً لعینَی

کَ وإعصار لھفۃٍ وشرودِ

آہِ ھل غابَ عن ظلام حیاتي

کلّ ما کان لھفۃً وفتونا ؟

کیف ضاع الحبُّ الإلھيّ یا طا

ئري الحرّ فانفجرت ظنونا ؟

 

وأنا لم أزلّ فؤاداً علی الشو

قِ يداري([2] ) غرامہُ المدفونا([3])

لیتني کنت بحتُ یا حُلمَ الرو

حِ وأعلنتُ حبّي المکنونا([4] )

کیف مرّت أیّامنا کیف مرّت

بین فکّ الأشواقِ والأحزانِ ؟

ملء قلبي وقلبکُ الحبُّ والشّوْ

قُ ولکن نلوذ بالکتمان

کلّما حدّثتکَ عیناي بالحبّ

أعاقبُ عینيَّ بالحرمانِ

کیف یا شاعري کتمنا ولم یَع

ص کیوبیدَ قبلنا عاشقانِ؟

کیف ضاعتُ عواطفي؟ کیف أنسَو

کَ غرامي وحیرتي ووفاءي؟

ملأوا قلبک النبیل أباطي

ل وصاغوا كواذبَ الأنباء

وقضیتُ الأیّام أذرف إحسا

...

Price Environment for Gold and Silver in the Context of the Development of Covid-19

The precious metals market is an integral part of the overall financial market. This part of the financial market plays an important role in the redistribution of financial flows. The precious metals market is currently under the influence of factors related to the spread of the COVID-19 pandemic. We investigated the dynamics of gold and silver prices in the context of the current pandemic development. For this analysis, we use the wavelet ideology. The results can be used in exchange trading in precious metals, investing in gold and silver.

The Use of Effective Questioning to Enhance Students Active Participation in a Grade - Xi Chemistry Classroom of a Government Degree College in the Rural District of Jacobabad

The aim of this study was to explore the usage of effective questioning during lecture-based teaching in Grade XI chemistry classroom in order to enhance students’ active participation. Use of effective questioning is an integral part of the teaching-learning processes. Classroom questioning is an extensively researched subject. The high incidence of questioning as a teaching strategy and its consequent potential for influencing students’ participation in classroom activities have led many researchers to explore relationships between teaching via questioning method and students’ level of active participation. The current scenario of education in Pakistan suggests that the teacher-centered practices of teaching chemistry at higher secondary are dominant. Teachers only pass on factual knowledge to students. The students’ classroom participation is given less importance. Hence, this study explored the usage of effective questioning as a teaching strategy in enhancing students’ active participation in a Grade XI chemistry classroom, in a Government (Boys) Degree College, rural Sindh, Pakistan. Data about the impact of intervention has been collected in different stages of the study. At the pre-intervention stage, semi-structured interviews and classroom observations were conducted to explore existing practices of using effective questioning and level of students’ participation during lecture-based teaching. During the intervention, writing reflection and informal discussion were the tools to collect the data about the impact of using effective questioning for the enhancement of students’ active participation. The post-intervention stage identified the impact of the intervention. In addition, the reflective diary of the researcher, field notes and students’ works have been used as data collection sources. The research study describes that when effective questioning is employed in chemistry classroom, students actively participate in the teaching and learning processes. The study also highlighted some of the factors like planning in advance, asking higher order questions, friendly environment, increasing wait time, and giving proper feedback, are helpful in enhancing students’ active participation by using effective questioning. On the contrary, large class size, teacher’s professional capacity to bring change and his workload were the challenging factors which hindered the use of effective questions. To conclude, findings of the study support the use of effective questioning because it not only raises the level of students’ active participation but also develops interest among them for their learning. The findings might prove useful to further the understanding of teaching and learning practices in chemistry classroom in the context of the professional development of chemistry teachers. It also provides