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Home > Downstream Approaches for Pure Biofine Chemicals and Aroma Substances from Cannabis Sativa Essential Oil

Downstream Approaches for Pure Biofine Chemicals and Aroma Substances from Cannabis Sativa Essential Oil

Thesis Info

Access Option

External Link

Author

Naz, Saima

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9725/1/Saima_Naz_Chemistry_UAF_2016.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725925573

Similar


Essential oils are complex mixtures of biologically active substances used since along time as flavoring agents and constituents of number of commercial products. Currently, their importance is more highlighted due to their increasing demand for food,cosmetics and pharmaceutical industries. Recent scientific literature revealed theantimicrobial, antifungal, antioxidant and pharmacological potential of essential oils. In view of themultiple applications of essential oils, their characterization based on their chemicalprofiles, is of great importance.In the present study, indigenous specie of Cannabaceae family “Cannabis sativa” was screenedfor the isolation of essential oils. Essential oil components were fractioned, separated and isolated. The variations in the yield, chemical composition and biological activities of theessential oils and fractions were investigated in the present study with respect to temperature and technique of extraction.The hydro-distilled, steam distilled and supercritical fluid extracted essential oils were characterized fortheir physico-chemical and biological activities. It was tried to purify and separate the major constituents of Cannabis sativa essential oil by vacuum fractionation. GC-MS analysis of thechemical constituents of the isolated oils and fractions mainly revealed the presence of monoterpene hydrocarbons, sesquiterpene hydrocarbons, oxygenated monoterpenes, and oxygenated sesquiterpenes. The investigated essential oils and fractions exhibited good antioxidant, antimicrobial, cytotoxic, insecticidal, anti-inflammatory, antitumor, gastroprotective, nephroprotective, hepatoprotective activities as measured by different in- vivo and in-vitro assays and experimental models.
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المقدمة

الحمد لله رب العالمين الصلاة والسلام على سيدنا محمّد الأمين - صلى الله عليه وسلم- المبعوث رحمة للعالمين وعلى آله وأصحابه أجمعين ومن تبعهم بإحسان إلى يوم الدين.

 أما بعد:                    

فلا شكّ أنَّ البشرية، لم تعرف كتابًا حظي بالدرس، والتمحيص، واحتفاء الناس به، كما هو الحال مع (القرآن العظيم). فمنذ تنزل الوحي على قلب نبينا - صلّى الله عليه وسلّم - لم يتوقف تأثير هذا الكتاب على قـُلوب الناس، وعقولهم؛ وذلك لأنّه نزل مفصلاً، قيمًا، متصفـًا بمعجز النظم كما في قوله تعالى: ﴿لَا يَأْتِيهِ الْبَاطِلُ مِنْ بَيْنِ يَدَيهِ وَلَا مِنْ خَلْفِهِ تَنْزِيلٌ مِنْ حَكِيمٍ حَمِيدٍ﴾ (فصلت: 42). جعله الله متلوًا لا يمل على طول التلاوة، وعجيبًا لا تنقضي عجائبه.

وقد أنزل الله - عزّ وجلّ - القرآن الكريم بلسانٍ عربيّ مبين، وجعلهُ محكمًا فقال:﴿كِتَابٌ أُحْكِمَتْ آيَاتُهُ ثُمَّ فُصِّلَتْ مِنْ لَدُنْ حَكِيمٍ خَبِيرٍ﴾ (هود: 1). وجعله معجزًا؛ تحدى به العرب، أنْ يأتوا بمثله، أو بسورة من مثله فقال سبحانه وتعالى: ﴿فَأْتُوا بِسُورَةٍ مِنْ مِثْلِهِ وَادْعُوا شُهَداءَكُمْ مِنْ دُونِ اللَّهِ إِنْ كُنْتُمْ صَادِقِينَ﴾ (البقرة: 23). وحكم عليهم بالعجز أنْ يفعلوا ذلك في الماضي، أو الحاضر، أو المستقبل فقال: ﴿فَإِنْ لَمْ تَفْعَلُوا وَلَنْ تَفْعَلُوا فَاتَّقُوا النَّارَ الَّتِي وَقُودُهَا النَّاسُ وَالْحِجَارَةُ أُعِدَّتْ لِلْكَافِرِينَ﴾  [االبقرة: 24]. ولم يكن هذا التحدي للعرب الذين اشتهروا بالفصاحة وحدهم، بل كان للإنس والجنّ قاطبةً، يقول تعالى: ﴿قُلْ لَئِنِ اجْتَمَعَتِ الْإِنْسُ وَالْجِنُّ عَلَى أَنْ يَأْتُوا بِمِثْلِ هَذَا الْقُرْآنِ لَا يَأْتُونَ بِمِثْلِهِ وَلَوْ كَانَ بَعْضُهُمْ لِبَعْضٍ ظَهِيرًا﴾ [الإسراء: 88].

أما الفصاحة لهذه الايات الكريمة تنمي إلى أدب القرآن فالآداب لا تكون في الإنسان إلا شرائع، ولكن الإنسان إذا عَرِيَ من الأدب النفسي، فربما شرع لنفسه ما لا يصنع الشيطانُ أخبثَ منه بل ما يَركضُ فيه الشيطانُ ركضاً؛ وقلما انتفعَ مَن لا أدب له بشريعة من الشرائع وإن كانت في الغاية التي لا مذهبَ وراءها...

The Rise of Greek Philosophy in the Muslim World and Imam Ghazali’s Tahafat-ul-Falasafah (An Analytical Study)

Imam al-Ghazali has criticized not the logical principles but their misuse and incompetence of philosophers. In addition, Imam al-Ghazali's objective position on philosophy and science in the objectives of the philosopher is highlighted that he had no problem with mathematics and in his view the difference in logic is actually the terminology. Imam al-Ghazali's personality has been debated in various contexts in the context of religion, philosophy and science, and even today new aspects of it keep coming to the fore. The reason for this is on the one hand Imam Sahib's intricate personality and on the other hand Imam Sahib's versatility. Modern studies have made Imam al-Ghazali an enemy of philosophy and science. Along with Orientalist wisdom, modern Islamic scholars also studied Imam al-Ghazali on the methodology of Enlightenment, due to which they too came to the same conclusion that Imam al-Ghazali is the biggest obstacle in the way of philosophy and science in Islamic civilization. Following in his footsteps, our traditionalist scholars now present Imam al-Ghazali as the victorious philosopher. No, but absorbed it. In this article, we have tried to make it clear that integration is a prominent element of Ghazali's philosophy of thought. Rejection of philosophy has become the identity of Imam Ghazali while passion for philosophy is a sign of his greatness of thought. Imam Ghazali's critique of Tahafat-ul-Falasfa defines the nature and scope of this critique. The main target of Imam Sahib's criticism was the theological conclusions of Muslim philosophers which contradicted the basic tenets of Islam. In addition, Imam al-Ghazali's objective position on philosophy and science in Maqasid al-Falasfa highlights that he had no problem with mathematics and in his view the difference in logic is in fact terminology. There is truth and falsehood in physics. We have to think about it. Yes, there is a lot of falsehood in theology. Imam al-Ghazali is the guarantor of the life of philosophy in theology. This article examines in detail the nature of the relationship between Imam al-Ghazali and philosophy, and how philosophy has influenced Imam Sahib's overall thought. The influence of philosophy on his theology, ethics, theology and principles of jurisprudence has been highlighted. At the same time, the effects of Imam Ghazali on philosophers and philosophers on Imam Ghazali are also presented with examples.

Investigations on Chrysoperla Carnea Stephens Neuroptera: Chrysopidae As a Biological Control Agent Against Cotton Pests in Pakistan

Studies on effect of different hosts on biology of Chrysoperla carnea (Stephens) carried out under laboratory conditions at 26±2°C and 65±5% R. H. indicated that the incubation period of eggs of C. carnea females feeding on different hosts as larvae was significantly (P <0.001) different from each other. The order of larval period on different prey species was Sitotroga cerealella > Aphis gossypii > Phenacoccus solenopsis > mixed host diet > Pectinophora gossypiella > Helicoverpa armigera.The maximum (100%) and minimum (50%) survival to adult stage was recorded on S. cerealella and P.gossypiella as hosts. The highest fecundity per female (503.3±9.17) and fertility (85.61±0.68) of eggs were recorded for females reared on S. cerealella eggs as a larval diet. C. carnea larvae consumed maximum amount of food when feeding on S. cerealella eggs followed by A. gossypii. The insects reared on artificial diet laid more eggs which were significantly more fertile than the eggs of insects feeding on S. cerealella. Survival to adult stage was also significantly higher for those insects feeding on artificial diet as larvae. Larval density had significant (P <0.001) effect on larval and pupal duration, pupal weight and percent adult emergence. When C. carnea was reared on different temperature regimes, 26°C was found to be the optimum temperature regime with significantly higher pupal recovery, adult emergence, fecundity and fertility of eggs. Experiments conducted at lower temperature regimes for extending the shelf-life of C. carnea indicated that duration of storage and temperature significantly affected egg survival. No egg survived when stored at 5°C. Egg stored at 7 and 9°C had 66.0 and 71.52% survival and all eggs were killed when stored for more than one week. Lower temperature had negative effect on survival of first instar C. carnea. Second and third instar C. carnea larvae showed better survival at lower temperature during storage compared with first instar. Compared with eggs and larvae, pupal stage of C. carnea was more sensitive to lower temperature regimes. Duration of storage and temperature adversely affected fecundity of females and fertility of eggs. Larval mass-rearing methods indicated that rearing of individual larva in plastic tubes yielded highest larval survival and percent adult emergence compared with other two methods. Different concentrations of various proteins in the artificial adult diet of C. carnea had a significant (P <0.001) effect on fecundity and fertility of eggs. The highest fecundity (785.12±25.75) and fertility (89.23±0.36) of eggs was recorded for adults feeding on diet containing Nu lure (5.0 ml diet -1 ). Among all colours tested, C. carnea females preferred black colour as a substrate for egg-laying and laid the highest (91.00%) xieggs. C. carnea laid highest number of eggs when a sex ratio of 1: 3 (male: females) was maintained. Radiation of C. carnea larvae and pupae affected the biological parameters. Larval period were extended when larvae were irradiated as first, second and third instars at 5 Gy. At higher dose levels, the biological parameters were affected negatively. Toxicity of insecticides against eggs of C. carnea varied significantly. Spinosad was non-toxic causing no egg mortality, abamectin was slightly toxic causing 2.5% mortality, while methomyl was highly toxic insecticide causing 26.25% mortality at field application dose rate. When C. carnea larvae and adults were exposed to insecticides, methomyl was found the most toxic and spinosad the least toxic insecticides. C. carnea egg cards were released in cotton (cv. NIAB-78) field for population management of sucking insect pests. Aphid, Aphis gossypii population reduction in predator released treatments was significant (P <0.001). By the end of season predator was able to cause 76.13 and 75.02% population reduction of aphid during 2005 and 2006, respectively. Release of C. carnea cards in cotton crop significantly (P <0.001) reduced the population of jassid, Amrasca devastans; thrips, Scirtothrips dorsalis and whitefly, Bemisia tabaci. The level of population reduction varied between different pest species. Population reduction was 83.70 and 76.07% for jassid; 37.59 and 60.32% for thrips and 51.84 and 44.08% for whitefly during 2005 and 2006, respectively. Release of C. carnea egg cards also had a significant effect on resident population of C. carnea in predator released treatments compared with control treatments. When C. carnea larvae were released in field cage study, third instar larvae were significantly (P <0.001) more effective than first and second instars in reducing sucking insect pest population in cotton.