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Home > Dynamics of Osrglp1 Gene During Fungal Exposure in Transgenic Potato

Dynamics of Osrglp1 Gene During Fungal Exposure in Transgenic Potato

Thesis Info

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Author

Majeed, Nadia

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7342/1/Nadia_Majeed_Biochemistry_2016_HSR_PMSA_13.02.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725931861

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Germin like proteins (GLPs) are large group of related and ubiquitous plant proteins. These proteins are considered to be involved in most of the processes important for the development of plant and defense mechanism. Although multiple copies of this gene family have been reported in a number of species (wheat, barley, rice, soybean, mosses and liverwort), even then the up-regulated expression of GLP transgenes has demonstrated additional defense capabilities. Due to reported role of GLPs in conferring fungal resistance, there is a need to explore their antifungal activity. For this purpose potato was selected as experimental material. It is a vegetable crop and produces, on average, additional food energy and protein than cereals. Potato with immense nutritive value, large yield, and significant cash return to farmers, has become a major crop for both farmers and consumers in Pakistan. Potato is susceptible to many kinds of diseases, especially to fungal pathogens, therefore genetic engineering of potato for disease resistance is an important strategy study and apply disease resistance. GM technology can be an effective tool for crop improvement. The transgenic approach was pursued to introduce rice (Oryza sativa) germin like protein gene OsRGLP1 using two recombinant vectors pC:OsRGLP1 and pG:OsGLP1 via Agrobacterium mediated transformation. Confirmation of presence of gene was carried out by polymerase chain reaction (PCR). One-step reverse transcriptase PCR was used for transcriptional analysis and expression was quantified in real time in all PCR positive putative transgenic lines. Functional status of transgene was studied in selected high expression lines from two vectors. It was observed that OsRGLP1 possesses super oxide dismutase activity and it is heat resistant and is sensitive to H2O2. These characteristics make it a Fe like SOD. A high H2O2 level was detected in transgenic lines. Fungal assay with Fusarium oxysporum f. sp. tuberosi showed xvi enhanced foliar resistance in transgenic lines in comparison to untransformed control plants. OsRGLP1 may be used as source for nonspecific fungal resistance in plants and the antioxidant activity of heat resistant SOD may be explored for abiotic stress tolerance.
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دوسرا باب: موسیؑ سے ربیوں تک

دورِ خروج

باب دوم کے اہم نکات

  1. عہد خروج میں عبرانیوں کی حالت۔
  2. قاضیوں کے دور کی اہمیت۔
  3. داؤدؑ و سلیمانؑ کے ادوار۔
  4. متحدہ بنی اسرائیل کی تقسیم۔
  5. شمالی و جنوبی ریاست کی تباہی۔
  6. جلا وطنی میں گزرا بابلی و فارسی دور۔
  7. ہیکل کی تعمیر نو۔
  8. یونانی دور میں یہودیوں کے حالات۔
  9. مکابیوں کی مختصر حکومت۔
  10. رومی دور اور مسیحیت کا عروج۔
  11. تلمود کی تشکیل۔
  12. بابل میں ربیائی یہودیت کا استحکام۔

 

عبرانی بائیبل سے پتہ چلتا ہے کہ عبرانی قوم جب مصر میں آباد تھی تو ان کی تعداد میں کافی اضافہ ہو گیا جس پر مصر کا بادشاہ فکر مند ہوا۔ اس نے اپنے سپاہیوں کو حکم دیا کہ ہر نئے پیدا ہونے والے عبرانی لڑکے کو قتل کر دیا جائے تاکہ ان کی تعداد نہ بڑھ سکے۔ عبرانیوں کا چار سو سال فرعونوں کی غلامی میں رہنے کے بعد مصر سے نکل کر کنعان جانا خروج کہلاتا ہے۔ یہ دور تقریباً 1275- 1250 قبل مسیح پر محیط ہے۔ توریت کی دوسری کتاب بھی اسی نسبت سے "خروج" کہلاتی ہے۔

موسیؑ اور بنی اسرائیل کی تشکیل نو

رمسیس/رعمسیس/رامسیس/رمیسس دوم (1290–1224 ق۔ م) کے دور تک یہی روایت چلی آتی رہی کہ اسرائیلیوں کے بیٹوں کو قتل کر دیا جاتا اور ان کی بیٹیوں کو زندہ چھوڑ دیا جاتا تاکہ وہ مصریوں کی باندیاں بنیں۔ بنی اسرائیل میں عمرام/عمران اور یوکابد نامی میاں بیوی رہا کرتے تھے جن کے ہاں موسیؑ (1300-1271 ق۔ م) کی پیدائش ہوئی جو بعد میں اپنی قوم کے نجات دہندہ بنے۔ توریت کے بیان کے مطابق تین ماہ تک ان کی والدہ یوکابد نے ان کو چھپا کر رکھا تاکہ فرعون کے...

بلاغة القرآن الكريم وتحدت ترجمة المعاني القرآنية الثانية إلى اللغة الأردية

His research paper is meant to explain the difficulties of translation of rhetorical and implicit meanings of Qur᾽ān into Urdu language as the translation of such meanings of Qur᾽ān is seriously a difficult job for reasons including the tacit relationship of meanings with the words’ structure and because these meanings take effect of social and mental characteristics of a particular group of people speaking a common language and also for the reason that the meanings change their position with the incessantly changing condition of mind and culture. Given the above facts the writer put forth analysis of four Urdu translations of Sūrah Al-Ḍuḥā after having studied their rhetorical aspects and implicit meanings in interpretations of different interpreters. The translations are as under. 1. Translation of Shaykh Abdul Qādir (d. 1233 A. H) named as “Maudihul Qur᾽ān”. 2. Translation of Aḥmad Raḍa Khan Braylvi (d. 1340 A. H) named as “Kanz ul ᾽Īmān fi Tarjama Al-Qur᾽ān” 3. Translation of Ashraf ‘Alī Thānvi (d. 1362 AH) with the name “Bayān Al-Qur᾽ān”. 4. Translation of Abdul Mājid Daryā Abādī (d. 1977 A. D) which is in literary style with attractive language. The writer has highlighted the lapses and shortcomings of these translations in rhetorical perspectives of Qur᾽ān and its implicit meanings and has also underlined the difficulties faced by these translators in a descriptive table. Findings and suggestions are given at the end.

Development of New Image Fusion Techniques

Image fusion techniques merge the complementary information of several images (multi-focus, multi-exposure and multi-modal). Each of these scenarios poses different challenges for image fusion techniques, which are being extensively researched. However, most of these works assume that source images are preregistered, which is a less practical scenario. Both registered and unregistered image fusion algorithms are considered in this thesis. The registration involves the geometrical / spatial alignment of source images taken using different sensors or a sensor in different operating conditions. This research is concerned with the reliable fusion schemes of several scenario images (including muti-focus, Infra Red (IR) and visible, Computed Tomography (CT) and Magnetic Resonance (MR), and multi-exposure images) demonstrating high quality fused results without loss of useful information. The first scheme is a textural registration based multi-focus scheme involving the Gabor filtering (with specific frequency and orientation) for extracting texture features from the images. The filtered images are aligned/registered using affine transformation. Noise and blur play an important role in image fusion and need to be classified and treated for quality image fusion. The next two fusion schemes deal with multi-exposure noisy (real and synthetic both) and blur images. In the first algorithm, the noisy, blurry and clean images are classified using Laplacian filter and histogram spread. The noise is reduced in the frequency domain. Heavy weights are assigned to noise free pixels and the blur images are passed through the Wiener filter. In the second algorithm, a noise resistant image fusion scheme for multi-exposure sensors using color dissimilarity (for motion detection and removal), median and noise maps is proposed. A well exposed image is obtained as a result of weighted average of multi-exposure source images. Higher valued weights are assigned to pixels containing low values of noises, high values of color dissimilarity and median maps. The next work (two schemes) involve pre-registered visible and IR images. In the first one, a three stage image fusion scheme using Genetic Algorithm (GA) is presented. In the first stage, it segments the image into homogeneous regions and generates segmentation maps. In the second stage, the segmentation maps are combined by an adaptive weight adjustment procedure. The third stage fuses the input images and segmentation maps via GA based multi- objective optimization strategy. The second image fusion scheme uses Un-Decimated Dual Tree Complex Wavelet Transform (UDTCWT) for astronomical images. The UDTCWT reduces noise effects and improves object classification due to its inherited shift invariance property. Local standard deviation and distance transforms are used to extract useful information, especially small objects. In the medical (CT and MR) image fusion scheme, the source images are contrast enhanced using histogram equalization. It is a sparse decomposition based fusion technique that uses the dictionary learnt from input images and k-mean singular value decomposition algorithm. The scheme splits CT and MR images into texture and gradient images. The texture decomposition improves the overall performance of the sparse representation based fusion. The quantitative analysis performed using mutual information, structural similarity measure and edge dependent based performance metrics, yields improved results for proposed schemes, as compared to existing schemes.