یہ تصنیف 1932 ء میں منظر عام پر آئی ۔ یہ کتاب اطالوی شاعر ”دانتے “کی”ڈیوائن کامیڈی“ کے انداز پر لکھی گئی۔ اقبال نے مولانا جلال الدین رومی کے ہمراہ افلاک کی سیر کی ہے اور مختلف مذہبی رہنماؤں اور دانشوروں سے ملاقات کی ہے۔ آخر میں جاوید سے خطاب ہے جو دراصل تمام نو جوانوں کے لیے پیغام ہے۔ اس تصنیف سے اقبال کے مطالعہ کی وسعت کا اندازہ ہوتا ہے خاص طور پر ہندی فکرو فلسفہ کے حوالہ سے اقبال کا مطالعہ کس قدر وسیع تھا۔ ہندوستانی قوم کے ماضی کی جھلک پر نظر ڈالی جاسکتی ہے اور جو لوگ قوم اور وطن کے ساتھ غداری کرتے ہیں ان کا کیا انجام ہوتاہے۔ ان کی حالت بھی دکھائی گئی ہے۔
کہیں ابو جہل کا نوحہ ہے تو کہیں فرعون کی روح ہے۔ کہیں ایک عورت پیغمبری کا دعوئی کرتی نظر آتی ہے اور شوہروں سے آزادی کا پیغام دیتی ہے تو کہیں ابلیس انسان کی کمزوری اور اپنی آسان کامیابیوں پر کسی مرد حق کی خواہش کرتا نظر آتا ہے۔ کہیں غدار جعفر بنگالی اور صادق دکنی خونی قلزم کے عذاب میں پڑے نظر آتے ہیں۔
ایسے افکار و نظریات اقبال کے علاوہ کہیں اور دیکھنے کو نہیں ملتے ۔ جاوید نامہ اقبال کی شہرہ آفاق تصنیف ہے جس نے اقبال کا خون تک نچوڑ لیا تھا تب کہیں جا کر یہ مکمل ہوئی۔
Ahmad Showqi is an eminent poet in this era, and he is also leader in Egypt, Arabic World and Europe. He is pioneer in Modern Arabic History. He followed his mentor Mahmood Saami Al-Baroodi. He spent his life in grooming. Arabic thinking and was beginner and elevator of Arabic poetry. He highlighted great civilization of Arabs. This modern movement of Arabic literature is zeal and zest of this period. This article reveals a profound academic and research study of Ahmad Showqi’s poetry. The main focus of the study is the Words and Verses of Holy Quran used by Ahmad Showqi in his poetry. Ahmad Showqi has presented before us a very fine interpretation and true meanings of the Quranic words. Similarly through his poetry he has presented before us the Quranic verses and passages in an effective way. So we may describe his poetry as an interpretation of the Holy Quran.
Ajuga bracteosa is a valuable yet endangered medicinal plant containing many important compounds, including withanolides, phytoecdysteroids, neo-clerodane and iridoid glycosides. These compounds possess a broad spectrum of biological, pharmacological and medicinal properties, but their yield is very low in wild A. bracteosa and chemical synthesis is not viable. The increased demand of natural products for medicinal purposes coupled with the low product yields and supply concerns of plant harvest has renewed interest in large-scale in vitro plant culture technology. A.bracteosa plant was subjected to in vitro culturing with the aim to study the effect of plant growth regulators on somatic embryogenesis for mass production and uniform plantlets for further genetic transformation. Leaf explants cultured on B5 medium containing 1mg/L 1-naphthaleneaceatic acid (NAA) + 0.5 mg/L 6-Benzyl amine purines (BAP) proved to be the most effective combination for somatic embryo induction. Agrobacterium tumefaciens strain GV3101 harboring pPCV002-A and pPCV002-CaMVC was used to obtain transgenic A. bracteosa plants. Transformation efficiency of 38% for rolA gene and 31% for rolC was obtained. Molecular analysis such as PCR and Southern blotting confirmed the integration of the rolA and rolC genes in the putative transgenic lines. The transcripts of these two genes were monitored by semi-quantitative RT-PCR. Furthermore, a significant difference in phytochemistry and the pharmacological activities (antioxidant, antibacterial, antiproliferative and antidiabetic) was observed not only between the transgenic and control/untransformed plants but also among the transgenic lines themselves. The rolC transgenic lines in general showed better results compared to rolA. Quantitative analysis of Electro-spray ionization mass spectrometer (ESI-TOF) revealed relatively higher contents of 20 hydroxyecdysone (20-HE) in transgenic plant lines of rolA and rolC compared to control/untransformed plant. Effect of low temperature stress on 20-HE biosynthesis revealed relatively high 20-HE content at 15°C compared to control (25°C). Principal component analysis (PCA) of the metabolic profiles obtained with non-targeted (ESI-MS) approach successfully distinguished the control A. bracteosa from the transgenic rolA and rolC population. Defense related secondary metabolites (tryptophan, mannitol, Cinnamic acid, and Shikimic acid) were identified in transgenic A. bracteosa lines. Taken together, our study suggests that low temperature stress and transformation with rol genes can be used for increased, constant and permanent production of valuable secondary metabolites from A. bracteosa plant.