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Effect of Processing Methods and Antioxidants on the Reduction of Aflatoxins in Red Chillies Capsicum Annuum L.

Thesis Info

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Author

Iqbal, Shahzad Z.

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2011

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1885

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725989977

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Chili is an important spice and used as salad, complementary dish, medicine, industrial product, or as decoration. It is consumed as fresh, dry, powder, paste or as sauce. It enhances food palatability, inducing the consumption of other foods. It is a rich source of Vitamin A & C. In Asia, chili is an important component in the cropping system. In terms of area, it is ranked first in Asia and third in the world as most important vegetable. Chilies are subject to various pest and disease constraints to optimal production. The contamination of the crops with mycotoxins from the growth of Aspergillus flavus and/or Aspergillus parasiticus is one of the most serious problems. Mycotoxins are secondary metabolites of fungi. Due to the widespread distribution of fungi in the environment, mycotoxins are considered to be one of the most important contaminants of foods and feeds. Aflatoxins are important class of mycotoxins. The toxins can be produced in the field prior to harvest, or alternatively, can arise due to fungal growth under poor storage conditions. Mycotoxins can produce both in temperate and tropical regions of the world, depending on the species of fungi. The extraction, clean up and analysis methodology for aflatoxins in chilies were validated and found it efficient and good in respect of recovery, reproducibility, repeatability, resolution and cost-effective. The collected chili samples from Faisalabad Division, Pakistan exhibited high level of aflatoxins but after treatment with γ-irradiation, more than 95% reduction in AFB 1 and ≥ 97% in Total (∑AFB 1 + AFB 2 +AFG 1 + AFG 2 ) were observed at a dose of 6 kGy. Citric acid was effective for the reduction of AFs in chilies and reduced 89% AFB 1 and 90% total AFs in contaminated chilies. Propionic acid is also used for the reduction of AFs in red chilies and it reduced 80% AFB 1 and 76% total AFs in chilies. Antioxidants like butylated hydroxyl-anisole (BHA) and butylated hydroxyl-toluene (BHT) were effective to reduce fungal load and the of (20:20 mM) of BHA and BHT were inhibit 100% of fungal load in malt extract agar of chilies. From the results it is evident that among aflatoxin reduction techniques, γ-irradiation is a good technology to remove contaminants like AFs from chilies. The food quality of irradiated cereals was evaluated to study any chemical change in vitamin E and fungal biomass. The total biomass (CFU g -1 ) showed linear behavior as increasing the dose level of gamma irradiation. In some samples of chilies, no microflora (fungi) was observed after 72 h. of incubation. From the results however, it is revealed that with increasing γ-irradiation doses a decrease in vitamin E content was observed in chilies but this decrease is not significant (p > 0.05). In the present study, phenolics, flavonoids and antioxidant activity of chilies and selected spices (cloves, turmeric, oregano, black pepper and cinnamon) have been evaluated in the Department of Food Science, Cornell University, USA. From the results, it has been evident that chilies and spices are good source of antioxidants. Special attention is urgently needed to create awareness of the high level of AFs contamination in chilies, among farmers, consumers, and traders. Special emphasis should also be given to adopt GHP and GSP in order to minimize the toxin in chilie
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حسرت ہی رہی مجھ پہ و ہ احسان کرے گا

حسرت ہی رہی مجھ پہ وہ احسان کرے گا
دیدار سے ہر درد کا درمان کرے گا

ہے عید کا دن آج وہ نکلے گا سنور کے
وہ عید کے دن حشر کا سامان کرے گا

بے چین مرے دل نہ رقیبوں سے ہوا کر
دشمن ہے تو ہر حال میں نقصان کرے گا

بیمارِ محبت ہے نکل جائے مطب سے
یہ اور مریضوں کو پریشان کرے گا

تائبؔ سے کبھی جان کو تو مانگ کے تو دیکھ
سو بار ترے نام پہ قربان کرے گا

COMPARISON OF REGIONAL MANUAL THERAPY AND STANDARD PHYSICAL THERAPY INTERVENTION IN FEMALES WITH SACROILIAC JOINT PAIN

Background and Aim: Sacroiliac joint pain is localized in the region of sacroiliac joint which can be increased by stress and provocation tests of the joint. Aim of this study was to compare two interventions for reduction of sacroiliac joint pain. Methodology: Study design was randomized clinical trial. Study was conducted in bajwah hospital and children polyclinic Lahore. Duration of study was six months. The total sample size was 64 patients. Females of 20-50 years old with diagnosed sacroiliac joint pain were included in this study. Compression and distraction objective tests were performed for further confirmation of sacroiliac joint pain.  Purposive sampling technique was used. Numeric pain rating scale (NPRS) and Oswestry low back disability questionnaire (ODI) were used to collect the data. Exclusion criteria was females with fractures and other abnormalities of spine. Results: Results showed that both groups were equal when assessed on baseline by normality test colmogorov-smirnova. Independent t test was applied to compare the mean value of NPRS. Pretreatment mean of NPRS scale for both the regional treatment and standard treatment groups was 7.After 4 weeks NPRS of regional treatment group was 4 and of standard treatment group was 7. The mean value of pretreatment ODI for regional treatment group was 33 and for standard treatment group was 34.After 4 weeks ODI of regional treatment group was 24 and mean of standard treatment group was 27. Conclusion: It is concluded that after giving equal sessions to both groups when results were assessed regional treatment is more effective than standard treatment.

Nonlinear Electromagnetic Waves in Dense Plasmas

The formation and propagation of electromagnetic waves, specifically the magnetoacoustic waves are studied in this thesis for dense electron-ion magnetoplasmas. The linear and nonlinear waves such as shocks and solitons for small but finite amplitude perturbations are described for various plasma models by taking into account the quantum magnetohydrodynamic model. Further, the possible magnetoacoustic solitary wave interactions, i.e., overtaking and head-on interactions are discussed. The results are numerically analyzed by choosing the plasma parameters consistent with compact astrophysical systems. The properties of nonlinear fast magnetoacoustic waves in dense dissipative magnetoplasmas with degenerate electrons are studied theoretically. For this purpose, the quantum magnetohydrodynamic equations and the reductive perturbation technique are employed to derive the Khokhlov-Zabolotskaya- Kuznetsov (KZK) equation. The assumptions to obtain KZK equation in plasma system and the limiting cases have been identified clearly. Shock solutions of KZK equation are obtained by employing a method based upon Hirota and Clarkson-Kruskal approach. The magnetoacoustic shock waves have been examined numerically to ascertain how the plasma parameters such as ion kinematic viscosity, number density and magnetic field alter the characteristics and dynamics of shock waves. The propagation characteristics of magnetoacoustic shock waves are further investigated in a dense magnetoplasma with spin-1/2 electrons and geometrical effects by deriving the planar Korteweg-de Vries Burgers (KdVB) and cylindrical KdVB equations. Numerically, cylindrical KdVB equation is analyzed and it is observed that the number density, magnetic field and ion kinematic viscosity are the parameters that bring about significant modifications in the structure and propagation of magnetoacoustic shock waves. The spin effects are found to mitigate the phase speed of magnetoacoustic waves and the amplitude of shock structures in a dense magnetoplasma. The amplitude of the shock wave is observed to be greater for the case of cylindrical geometry and is found to propagate faster than that of planar shock waves. Furthermore, the numerical results are compared with the approximate analytical solution to show an excellent agreement of the results in the limit of earlier times. Overtaking interaction of fast magnetoacoustic solitons in dense magnetoplasmas is investigated. In this regard, one dimensional propagation of magnetoacoustic solitary waves in electron-ion plasmas with degenerate electrons is considered by deriving the Korteweg-de Vries (KdV) equation. Numerically, the characteristics of solitary waves are studied by varying the plasma parameters i.e., number density and magnetic field. Hirota bilinear formalism is applied to get the multi-soliton solutions and overtaking interaction of fast magnetoacoustic solitons is explored by utilizing them. It is observed that the values of the propagation vectors determine the interaction of solitary waves. The taller soliton being faster, overtakes the shorter soliton such that the amplitude of the respective solitary waves remain unchanged after the interaction, however they do experience a phase shift. Further, the head-on interaction of two magnetoacoustic solitons is studied in a dense magnetoplasma with spin-1/2 electrons and geometrical effects. The extended Poincaré- Lighthill-Kuo (PLK) technique and quantum magnetohydrodynamic equations are utilized to derive a pair of nonplanar Korteweg-de Vries equations. The PLK method is an analytical approach, which explicitly provides the relations of post collision trajectories and the phase shifts encountered by the magnetoacoustic solitons after interaction. The head-on interaction of two concentric ring solitons is numerically analysed. It is observed that the spin-1/2 effects, statistical pressure, displacement current and geometry of the system significantly modify the phase shifts encountered by the solitons. It is noticed that the increasing the number density decreases the phase shift of the colliding solitons. Furthermore, the cylindrical geometry is observed to decrease the phase shift by comparison with the planar geometry. The investigations carried out in this thesis shall hopefully equip us to comprehend the formation, propagation and interaction of magnetoacoustic solitons in dense magnetoplasmas which exist in compact astrophysical objects like white dwarfs and neutron stars.