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Home > Effect of Radiation on Varietal Improvement of Regenerated Plants of Lilium and Their Characterization Through Molecular Markers

Effect of Radiation on Varietal Improvement of Regenerated Plants of Lilium and Their Characterization Through Molecular Markers

Thesis Info

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Author

Farah Aslam

Program

PhD

Institute

Lahore College for Women University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12501/1/Farah%20Aslam_Botany_2016_LCWU_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725991396

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Lilium is one of the six major bulbous genera crops and also one of the leading cut flowers all over the world. It has three important markets: fresh-cut flowers, potted flowering plants and bulbs for domestic gardens and formal landscapes. So keeping in view the importance of this ornamental plant, the present study was conducted to induce mutation (in vivo and in vitro mutagenesis) in different cultivars of Lilium to get the new varieties having better morphological traits including plant height, leaf and flower size, flower shape and flower colour. For this purpose an efficient protocol has been optimized for micropropagation of ten cultivars present in four major groups (Oriental, Oriental x Trumpet, Longiflorum x Oriental and Longiflorum) of Lilium to get the shoots that were further used as plant material for in vitro mutagenesis. MS medium fortified with BAP 0.1 + Kin 0.1 + IAA 3.0 (mg/L) has been found to be best for shoot initiation. Effect of shaking cultures and sucrose concentration was also observed in the present study. It was observed that cultures inoculated in the same medium but in agitation position with 120 (rpm) showed higher regenerated frequency as compared to stationary cultures. Similarly moderate sucrose concentration (60 g/L) proved to be better as compared to 30 and 90 (g/L). Healthy regenerated plantlets were acclimatized in sterile mixture of sand, soil and cocopeat in the ratio of 1:1:1. Mutagenesis was induced in all the groups of Lilium including by treating the bulbs (in vivo mutagenesis) and regenerated shoots (in vitro mutagenesis) through plant tissue culture during the first phase of current study with different doses of gamma rays 2.0-10.0 (Gy). It was noticed that growth of plants in both in vivo and in vitro mutagenesis was affected by gamma rays. Except in Samur La pink cultivar of Lilium, higher doses have inhibitory effect on plant growth, but in Samur La pink of Oriental group of Lilium, stimulatory effect was observed at higher doses of gamma rays. Superior mutants were selected on the basis of change in flower morphology. Larger flowers with smooth petals were obtained at 10.0 (Gy) dose of gamma irradiation. Similarly changes in flower morphology were also noticed in Montezuma-O-red, Advantage La salmon, Golden tycon yellow and Easter lily cultivars of Lilium at different gamma irradiation doses. It is evident from the results of present investigation that mutagenesis combined with plant tissue technique lead to varietal improvement of different cultivars of Lilium. Further study was also carried out to make the molecular characterization of control and mutants of same cultivar to detect the genetic polymorphism and variations. Two different molecular marker systems including Random Amplified Polymorphic DNA (RAPDs) and Simple Sequence Repeats (SSRs) were selected for the purpose. Among the molecular markers, Randomly Amplified Polymorphic DNA (RAPDs) was used as a marker of choice in the present investigation. It was noticed that mutagenesis caused changes at genetic level and separated the control genotypes from the irradiated ones into different groups depending on the genetic variation occurred at different doses. Mostly control and mutants treated at lower doses of gamma rays were combined in one group while genotypes treated at higher doses were separated in another group. Other molecular marker system selected for the present investigations was Simple Sequence Repeats (SSRs) due to high level of polymorphism and reliability. Genetic polymorphism along with the respective allele sizes, PIC (Polymorphic Information Content) values and Hobs (Observed heterozygosity) of the cultivars was also recorded. These markers also separated the control and mutant genotypes on the basis of doses of gamma rays like RAPDs. It is evident from the above results that mutagenesis (in vivo and in vitro) caused genetic variations proved by RAPDs and SSRs which lead to production of new varieties having improved morphological traits.
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خان بہادر ظفرحسین خان

خان بہادر ظفر حسین خاں
دوسرا حادثہ بہادر ظفر حسین خاں کی وفات کا ہے، وہ بھی اس علمی بزم کی یادگار تھے، اس لیے ان کے تعلقات بھی دارالمصنفین سے دیرینہ تھے، وہ انسپکٹر آف اسکولس کے عہدہ پر ممتاز تھے، اس سے ریٹائر ہونے کے بعد کچھ دنوں تک شیعہ کالج کے پرنسپل رہے، ادھر عرصہ سے عزلت نشینی کی زندگی بسر کررہے تھے، مگر تالیف و تصنیف کا سلسلہ آخر تک جاری رہا، ان کا خاص موضوع فلسفہ تھا اور اس کی تاریخ پر ان کی نظر بہت وسیع تھی، اس موضوع پر ان کی کئی تصانیف ہیں، ان کی ایک تصنیف مقالہ روسو عرصہ ہوا اور دوسری مال و مشیت ابھی چند سال ہوئے دارالمصنفین سے شائع ہوئی تھی، آخرالذکر کتاب پر ان کو ساہتیہ اکیڈمی سے پانچ ہزار انعام ملا تھا، انجمن ترقی اردو ہند سے بھی ان کی بعض فلسفیانہ تصانیف شائع ہوئیں، اﷲ تعالیٰ دونوں خدام علم کو مغفرت فرمائے۔
(شاہ معین الدین ندوی، اگست ۱۹۶۱ء)

الحكمة في شعر محمود سامي البارودي

Muḥmood Sami al Barodi is a famous poet who was named the Resurrector of poetry in the early times when many poets of the old era were the cause of the decline in poetry. His poems had been studied from many aspects and by many scholars but no one ever spoke about al Barodi’s writings about wisdom. This article is focusing on the verses and poems that focus on the meaning of wisdom and everything that relate to it. He talked about the importance of wisdom in the poems as he encouraged the other poets to give attention to this meaning. Some published books and articles helped me write this article but I haven’t found any of them that gave this point enough significance though his poetry is full of verses about wisdom and so I chose to write about it.

Impact of Karakoram Highway on the Socio-Economic Development of Gilgit-Baltistan, Pakistan

This study attempts to evaluate the impact Karakoram Highway (KKH) on the socio-economic development of Gilgit-Baltistan, Pakistan. It is a vast, mountainous and remote area in the extreme north of Pakistan extend between 340 40’ to 370 0’ North latitude and 720 30’ to 780 0’ East longitude. Geographically, the study area (Gilgit-Baltistan) is bounded in the North-West by Wakhan Corridor of Afghanistan, China in the North-East, Jammu and Kashmir in the East, Azad Jammu and Kashmir in the South-East, whereas the province of Khyber Pakhtunkhwa of Pakistan in the south and west. The climate of the region is characterized by warm/hot summer and cool/cold winter. The total area of Gilgit-Baltistan is about 72,496 km2. For the purpose of administration, it has been divided into seven districts i.e. Gilgit, Diamer, Hunza-Nagar, Ghanche, Ghizer, Skardu and Astore. Significance of the area is evident from its geographical location as it is the juncture of Central Asia, China and South Asia. However, this important area remained cut off from the rest of the country (Pakistan) until the inception of Karakoram Highway (KKH) in 1978. Their only source of livelihood was local agricultural production which was insufficient for the whole population. Due to scarcity of victuals large number of people faced starvation during the snowy winter season. The idea of constructing this gigantic highway was proposed after the boundary settlement with China in 1963. Construction of KKH started in 1966 aiming at two main objectives; to link the vast area of Gilgit-Baltistan with the rest of Pakistan and to develop a land route to China. This study is an attempt to sort out impacts of KKH and to answer the question ‘what are the impacts of KKH on the socio-economic development of Gilgit-Baltistan, Pakistan’. To achieve the task four objectives were designed. Primarily, to explore the growth and development of KKH, secondly, to assess the impact of KKH on the socio-economic condition of Gilgit-Baltistan, thirdly, to find out the future prospects of KKH for the socio-economic development of the study area. To carry out this study, six variables including population, land use and agriculture, education, health, trade and tourism were selected and tested. Keeping in view purpose of the study, it was essential to collect all the necessary data for the achievement of objectives. Data were collected both from primary and secondary sources. Data pertaining to selected variables were collected pre and post KKH as available for the whole area. The collected data were analysed and presented in the form of maps, statistical diagrams and tables. The analysis reveals that after the completion of KKH the inhabitants of Gilgit-Baltistan took a sigh of relief and positive changes occurred in the overall socio-economic sectors. It was explored from the analysis that the population of the districts along KKH has increased manifold. The agriculture and livestock sector was a traditional source of livelihood, but after the construction of KKH a paradigm shift has been recorded by introducing modern agricultural techniques and motivation for the farmers to cultivate more rewarding cash crops. The analysis further revealed that during 1996 to 2016 land use land cover in the three sample districts located along the KKH has shown radical changes. It was found that area under built-up environment was increased, contrary to this area under farmland decreased. Similarly, positive change has been noted in area under forest cover. It was found from the analysis that after the construction of KKH a rapid progress in education and health sector has been registered. The literacy ratio was rapidly increased from 14.7% in 1981 to 37.8% in 1998 and further increased to 52.0 percent in 2012. However, a recorded twelve-time increase is registered in women education, from 1981 (3%) to 2012 (36%). Nevertheless, the women literacy ratio is still very low in Diamer and Astore districts. Similarly, in Gilgit-Baltistan, the number of Government health institutions has increased. However, the limited number of trained doctors is still an issue to be overcome. The analysis revealed that the performance of maternal and child health services in Gilgit-Baltistan lags behind and appears to have been weak. The analysis revealed that there is expanding trade and commence potentials due to KKH and greater retention of economic value in the local economy is expected to further boost-up. However, it needs special attention of National Highway Authority to take care of its maintenance and to mitigate frequent landslides along the KKH. The study explores that prior to the construction of KKH the latitudes of trade between Pakistan and China through ancient silk route was negligible. However, after the inception of KKH, the trade between Pakistan and China has been boost-up. The recent development in the form of China-Pakistan Economic Corridor (CPEC), where KKH is part of CPEC project provides a land route to China and Gilgit-Baltistan to warm-water of Arabian Sea and expected to further boost-up the trade between China and Pakistan in the years ahead. It was found from the analysis that KKH has opened avenues for the local population. It is high time that the ministry of tourism and culture needs to develop tourism sector on priority basis and un-earthen this precious treasury of Gilgit-Baltistan. It was found from the analysis that prior to the construction of KKH, poor road network, lack of transport facility, absence of hotels and restaurants had been hurdles in promoting tourism. This study, however, provides policy guidelines for promoting socio-economic development of this underdeveloped area.